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Protein

Putative ubiquitin carboxyl-terminal hydrolase 50

Gene

Usp50

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei54 – 541NucleophilePROSITE-ProRule annotation
Active sitei321 – 3211Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.058.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative ubiquitin carboxyl-terminal hydrolase 50 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 50
Ubiquitin thioesterase 50
Ubiquitin-specific-processing protease 50
Gene namesi
Name:Usp50
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1922333. Usp50.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 390390Putative ubiquitin carboxyl-terminal hydrolase 50PRO_0000249529Add
BLAST

Proteomic databases

PRIDEiQ6P8X6.

PTM databases

PhosphoSiteiQ6P8X6.

Expressioni

Gene expression databases

BgeeiQ6P8X6.
CleanExiMM_USP50.
ExpressionAtlasiQ6P8X6. baseline.
GenevestigatoriQ6P8X6.

Structurei

3D structure databases

ProteinModelPortaliQ6P8X6.
SMRiQ6P8X6. Positions 43-361.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 363319USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5533.
GeneTreeiENSGT00790000122967.
HOGENOMiHOG000231498.
HOVERGENiHBG098592.
InParanoidiQ6P8X6.
OMAiECSLQDC.
PhylomeDBiQ6P8X6.
TreeFamiTF106277.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6P8X6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCFIDMASHR PVPADDFGAY YNLAECADYD SLPESKTQPH FQGVTGLRNL
60 70 80 90 100
GNTCYMNAIL QCLCSVSPLV EYFLSGKYIT ALKKDCSEVT TAFAYLMTDM
110 120 130 140 150
WLGDSDCVSP EIFLSAVGSL YPAFLKKTQQ DAQEFLIYVL NELHEALKKH
160 170 180 190 200
CRRRVNEKRT GQSCCRKVPA QETSIITRLF EGQLSYSITC LKCESCTHKN
210 220 230 240 250
EVFTILSLPI PSDYECSLQD CLQCFFQQDT LTWSNQIYCS FCEIKQEAAV
260 270 280 290 300
RTTISKVPKI IVFHFKRFDI QGTVKRKLRT DIHYPLTNLD LTPYICPVFR
310 320 330 340 350
KHPMYNLCAV VNHFGDLDGG HYTAFCKNSV TQAWYSFDDT RVSEIPDTSV
360 370 380 390
QTATAYLLFY SCQPFSIPAQ KRKSQDSTPD HCKQAIRKWP
Length:390
Mass (Da):44,543
Last modified:July 5, 2004 - v1
Checksum:i37D9FC61FF818E88
GO
Isoform 2 (identifier: Q6P8X6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: Missing.
     220-232: DCLQCFFQQDTLT → VGSPQLLNIFSCF

Note: No experimental confirmation available.

Show »
Length:335
Mass (Da):38,292
Checksum:i0334E33D5C9B5566
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti173 – 1731T → I in BAB29966 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5555Missing in isoform 2. 1 PublicationVSP_020486Add
BLAST
Alternative sequencei220 – 23213DCLQC…QDTLT → VGSPQLLNIFSCF in isoform 2. 1 PublicationVSP_020487Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007018 mRNA. Translation: BAB24831.1.
AK015766 mRNA. Translation: BAB29966.1.
BC061020 mRNA. Translation: AAH61020.1.
CCDSiCCDS16688.1. [Q6P8X6-1]
RefSeqiNP_083439.2. NM_029163.3. [Q6P8X6-1]
UniGeneiMm.389373.

Genome annotation databases

EnsembliENSMUST00000028842; ENSMUSP00000028842; ENSMUSG00000027364. [Q6P8X6-1]
GeneIDi75083.
KEGGimmu:75083.
UCSCiuc008mee.2. mouse. [Q6P8X6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007018 mRNA. Translation: BAB24831.1.
AK015766 mRNA. Translation: BAB29966.1.
BC061020 mRNA. Translation: AAH61020.1.
CCDSiCCDS16688.1. [Q6P8X6-1]
RefSeqiNP_083439.2. NM_029163.3. [Q6P8X6-1]
UniGeneiMm.389373.

3D structure databases

ProteinModelPortaliQ6P8X6.
SMRiQ6P8X6. Positions 43-361.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC19.058.

PTM databases

PhosphoSiteiQ6P8X6.

Proteomic databases

PRIDEiQ6P8X6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028842; ENSMUSP00000028842; ENSMUSG00000027364. [Q6P8X6-1]
GeneIDi75083.
KEGGimmu:75083.
UCSCiuc008mee.2. mouse. [Q6P8X6-1]

Organism-specific databases

CTDi373509.
MGIiMGI:1922333. Usp50.

Phylogenomic databases

eggNOGiCOG5533.
GeneTreeiENSGT00790000122967.
HOGENOMiHOG000231498.
HOVERGENiHBG098592.
InParanoidiQ6P8X6.
OMAiECSLQDC.
PhylomeDBiQ6P8X6.
TreeFamiTF106277.

Miscellaneous databases

NextBioi342171.
PROiQ6P8X6.
SOURCEiSearch...

Gene expression databases

BgeeiQ6P8X6.
CleanExiMM_USP50.
ExpressionAtlasiQ6P8X6. baseline.
GenevestigatoriQ6P8X6.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Testis.

Entry informationi

Entry nameiUBP50_MOUSE
AccessioniPrimary (citable) accession number: Q6P8X6
Secondary accession number(s): Q9D558, Q9D9E7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: July 5, 2004
Last modified: May 27, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The human ortholog is inactive.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.