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Q6P8X6

- UBP50_MOUSE

UniProt

Q6P8X6 - UBP50_MOUSE

Protein

Putative ubiquitin carboxyl-terminal hydrolase 50

Gene

Usp50

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei54 – 541NucleophilePROSITE-ProRule annotation
    Active sitei321 – 3211Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. ubiquitinyl hydrolase activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC19.058.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative ubiquitin carboxyl-terminal hydrolase 50 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 50
    Ubiquitin thioesterase 50
    Ubiquitin-specific-processing protease 50
    Gene namesi
    Name:Usp50
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1922333. Usp50.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 390390Putative ubiquitin carboxyl-terminal hydrolase 50PRO_0000249529Add
    BLAST

    Proteomic databases

    PRIDEiQ6P8X6.

    PTM databases

    PhosphoSiteiQ6P8X6.

    Expressioni

    Gene expression databases

    BgeeiQ6P8X6.
    CleanExiMM_USP50.
    GenevestigatoriQ6P8X6.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6P8X6.
    SMRiQ6P8X6. Positions 43-361.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini45 – 363319USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5533.
    GeneTreeiENSGT00750000117363.
    HOGENOMiHOG000231498.
    HOVERGENiHBG098592.
    InParanoidiQ6P8X6.
    OMAiECSLQDC.
    PhylomeDBiQ6P8X6.
    TreeFamiTF106277.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6P8X6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MCFIDMASHR PVPADDFGAY YNLAECADYD SLPESKTQPH FQGVTGLRNL    50
    GNTCYMNAIL QCLCSVSPLV EYFLSGKYIT ALKKDCSEVT TAFAYLMTDM 100
    WLGDSDCVSP EIFLSAVGSL YPAFLKKTQQ DAQEFLIYVL NELHEALKKH 150
    CRRRVNEKRT GQSCCRKVPA QETSIITRLF EGQLSYSITC LKCESCTHKN 200
    EVFTILSLPI PSDYECSLQD CLQCFFQQDT LTWSNQIYCS FCEIKQEAAV 250
    RTTISKVPKI IVFHFKRFDI QGTVKRKLRT DIHYPLTNLD LTPYICPVFR 300
    KHPMYNLCAV VNHFGDLDGG HYTAFCKNSV TQAWYSFDDT RVSEIPDTSV 350
    QTATAYLLFY SCQPFSIPAQ KRKSQDSTPD HCKQAIRKWP 390
    Length:390
    Mass (Da):44,543
    Last modified:July 5, 2004 - v1
    Checksum:i37D9FC61FF818E88
    GO
    Isoform 2 (identifier: Q6P8X6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-55: Missing.
         220-232: DCLQCFFQQDTLT → VGSPQLLNIFSCF

    Note: No experimental confirmation available.

    Show »
    Length:335
    Mass (Da):38,292
    Checksum:i0334E33D5C9B5566
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti173 – 1731T → I in BAB29966. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 5555Missing in isoform 2. 1 PublicationVSP_020486Add
    BLAST
    Alternative sequencei220 – 23213DCLQC…QDTLT → VGSPQLLNIFSCF in isoform 2. 1 PublicationVSP_020487Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK007018 mRNA. Translation: BAB24831.1.
    AK015766 mRNA. Translation: BAB29966.1.
    BC061020 mRNA. Translation: AAH61020.1.
    CCDSiCCDS16688.1. [Q6P8X6-1]
    RefSeqiNP_083439.2. NM_029163.3. [Q6P8X6-1]
    UniGeneiMm.389373.

    Genome annotation databases

    EnsembliENSMUST00000028842; ENSMUSP00000028842; ENSMUSG00000027364. [Q6P8X6-1]
    GeneIDi75083.
    KEGGimmu:75083.
    UCSCiuc008mee.2. mouse. [Q6P8X6-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK007018 mRNA. Translation: BAB24831.1 .
    AK015766 mRNA. Translation: BAB29966.1 .
    BC061020 mRNA. Translation: AAH61020.1 .
    CCDSi CCDS16688.1. [Q6P8X6-1 ]
    RefSeqi NP_083439.2. NM_029163.3. [Q6P8X6-1 ]
    UniGenei Mm.389373.

    3D structure databases

    ProteinModelPortali Q6P8X6.
    SMRi Q6P8X6. Positions 43-361.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C19.058.

    PTM databases

    PhosphoSitei Q6P8X6.

    Proteomic databases

    PRIDEi Q6P8X6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028842 ; ENSMUSP00000028842 ; ENSMUSG00000027364 . [Q6P8X6-1 ]
    GeneIDi 75083.
    KEGGi mmu:75083.
    UCSCi uc008mee.2. mouse. [Q6P8X6-1 ]

    Organism-specific databases

    CTDi 373509.
    MGIi MGI:1922333. Usp50.

    Phylogenomic databases

    eggNOGi COG5533.
    GeneTreei ENSGT00750000117363.
    HOGENOMi HOG000231498.
    HOVERGENi HBG098592.
    InParanoidi Q6P8X6.
    OMAi ECSLQDC.
    PhylomeDBi Q6P8X6.
    TreeFami TF106277.

    Miscellaneous databases

    NextBioi 342171.
    PROi Q6P8X6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q6P8X6.
    CleanExi MM_USP50.
    Genevestigatori Q6P8X6.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Testis.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Testis.

    Entry informationi

    Entry nameiUBP50_MOUSE
    AccessioniPrimary (citable) accession number: Q6P8X6
    Secondary accession number(s): Q9D558, Q9D9E7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2006
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The human ortholog is inactive.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3