Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q6P8X6 (UBP50_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative ubiquitin carboxyl-terminal hydrolase 50
EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 50
Ubiquitin thioesterase 50
Ubiquitin-specific-processing protease 50
Gene names
Name:Usp50
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length390 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Miscellaneous

The human ortholog is inactive.

Sequence similarities

Belongs to the peptidase C19 family.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Coding sequence diversityAlternative splicing
   Molecular functionHydrolase
Protease
Thiol protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncysteine-type peptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin thiolesterase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6P8X6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6P8X6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: Missing.
     220-232: DCLQCFFQQDTLT → VGSPQLLNIFSCF
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 390390Putative ubiquitin carboxyl-terminal hydrolase 50
PRO_0000249529

Sites

Active site541Nucleophile By similarity
Active site3211Proton acceptor By similarity

Natural variations

Alternative sequence1 – 5555Missing in isoform 2.
VSP_020486
Alternative sequence220 – 23213DCLQC…QDTLT → VGSPQLLNIFSCF in isoform 2.
VSP_020487

Experimental info

Sequence conflict1731T → I in BAB29966. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 37D9FC61FF818E88

FASTA39044,543
        10         20         30         40         50         60 
MCFIDMASHR PVPADDFGAY YNLAECADYD SLPESKTQPH FQGVTGLRNL GNTCYMNAIL 

        70         80         90        100        110        120 
QCLCSVSPLV EYFLSGKYIT ALKKDCSEVT TAFAYLMTDM WLGDSDCVSP EIFLSAVGSL 

       130        140        150        160        170        180 
YPAFLKKTQQ DAQEFLIYVL NELHEALKKH CRRRVNEKRT GQSCCRKVPA QETSIITRLF 

       190        200        210        220        230        240 
EGQLSYSITC LKCESCTHKN EVFTILSLPI PSDYECSLQD CLQCFFQQDT LTWSNQIYCS 

       250        260        270        280        290        300 
FCEIKQEAAV RTTISKVPKI IVFHFKRFDI QGTVKRKLRT DIHYPLTNLD LTPYICPVFR 

       310        320        330        340        350        360 
KHPMYNLCAV VNHFGDLDGG HYTAFCKNSV TQAWYSFDDT RVSEIPDTSV QTATAYLLFY 

       370        380        390 
SCQPFSIPAQ KRKSQDSTPD HCKQAIRKWP

« Hide

Isoform 2 [UniParc].

Checksum: 0334E33D5C9B5566
Show »

FASTA33538,292

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Testis.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AK007018 mRNA. Translation: BAB24831.1.
AK015766 mRNA. Translation: BAB29966.1.
BC061020 mRNA. Translation: AAH61020.1.
IPIIPI00420445.
IPI00461643.
RefSeqNP_083439.2. NM_029163.3.
UniGeneMm.389373.

3D structure databases

HSSPHSSP built from PDB template 2AYN based on UniProtKB P54578.
ProteinModelPortalQ6P8X6.
SMRQ6P8X6. Positions 43-361.
ModBaseSearch...

Protein family/group databases

MEROPSC19.058.

PTM databases

PhosphoSiteQ6P8X6.

Proteomic databases

PRIDEQ6P8X6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028842; ENSMUSP00000028842; ENSMUSG00000027364.
GeneID75083.
KEGGmmu:75083.

Organism-specific databases

CTD373509.
MGIMGI:1922333. Usp50.

Phylogenomic databases

eggNOGCOG5533.
GeneTreeENSGT00670000097591.
HOGENOMHOG000231498.
HOVERGENHBG098592.
InParanoidQ6P8X6.
OMAHFQGVTG.

Gene expression databases

ArrayExpressQ6P8X6.
BgeeQ6P8X6.
CleanExMM_USP50.
GenevestigatorQ6P8X6.
GermOnlineENSMUSG00000027364. Mus musculus.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
PROSITEPS00972. UCH_2_1. 1 hit.
PS00973. UCH_2_2. 1 hit.
PS50235. UCH_2_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio342171.
SOURCESearch...

Entry information

Entry nameUBP50_MOUSE
AccessionPrimary (citable) accession number: Q6P8X6
Secondary accession number(s): Q9D558, Q9D9E7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: July 5, 2004
Last modified: May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents