ID   HMGB4_MOUSE             Reviewed;         181 AA.
AC   Q6P8W9; Q9DAR4;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   24-JAN-2024, entry version 130.
DE   RecName: Full=High mobility group protein B4;
GN   Name=Hmgb4;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Testis;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND SUBCELLULAR LOCATION.
RX   PubMed=25609838; DOI=10.1095/biolreprod.114.126250;
RA   Petit F.G., Kervarrec C., Jamin S.P., Smagulova F., Hao C., Becker E.,
RA   Jegou B., Chalmel F., Primig M.;
RT   "Combining RNA and protein profiling data with network interactions
RT   identifies genes associated with spermatogenesis in mouse and human.";
RL   Biol. Reprod. 92:71-71(2015).
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:25609838}. Chromosome
CC       {ECO:0000269|PubMed:25609838}. Note=Interacts specifically with the sex
CC       chromosomes. {ECO:0000269|PubMed:25609838}.
CC   -!- TISSUE SPECIFICITY: Expressed in adult germ cells (at protein level).
CC       {ECO:0000269|PubMed:25609838}.
CC   -!- DEVELOPMENTAL STAGE: Strongly expressed in adult germ cells and weakly
CC       expressed embryonic gonads (PubMed:25609838). In meiotic germ cells,
CC       begins to accumulate during the leptotene stage, before its
CC       concentration increases further during the pachytene and diplotene
CC       stages, where it localizes to the sex body (PubMed:25609838).
CC       {ECO:0000269|PubMed:25609838}.
CC   -!- SIMILARITY: Belongs to the HMGB family. {ECO:0000305}.
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DR   EMBL; AK005588; BAB24141.1; -; mRNA.
DR   EMBL; BC061030; AAH61030.1; -; mRNA.
DR   CCDS; CCDS18671.1; -.
DR   RefSeq; NP_081312.2; NM_027036.3.
DR   AlphaFoldDB; Q6P8W9; -.
DR   SMR; Q6P8W9; -.
DR   STRING; 10090.ENSMUSP00000059625; -.
DR   PhosphoSitePlus; Q6P8W9; -.
DR   PaxDb; 10090-ENSMUSP00000059625; -.
DR   ProteomicsDB; 267053; -.
DR   Antibodypedia; 17205; 284 antibodies from 28 providers.
DR   DNASU; 69317; -.
DR   Ensembl; ENSMUST00000053830.5; ENSMUSP00000059625.4; ENSMUSG00000048686.5.
DR   GeneID; 69317; -.
DR   KEGG; mmu:69317; -.
DR   UCSC; uc008uva.1; mouse.
DR   AGR; MGI:1916567; -.
DR   CTD; 127540; -.
DR   MGI; MGI:1916567; Hmgb4.
DR   VEuPathDB; HostDB:ENSMUSG00000048686; -.
DR   eggNOG; KOG0381; Eukaryota.
DR   GeneTree; ENSGT00940000162735; -.
DR   HOGENOM; CLU_082854_0_4_1; -.
DR   InParanoid; Q6P8W9; -.
DR   OMA; MWSAKTD; -.
DR   OrthoDB; 1222485at2759; -.
DR   PhylomeDB; Q6P8W9; -.
DR   TreeFam; TF105371; -.
DR   BioGRID-ORCS; 69317; 3 hits in 75 CRISPR screens.
DR   ChiTaRS; Hmgb4; mouse.
DR   PRO; PR:Q6P8W9; -.
DR   Proteomes; UP000000589; Chromosome 4.
DR   RNAct; Q6P8W9; Protein.
DR   Bgee; ENSMUSG00000048686; Expressed in seminiferous tubule of testis and 13 other cell types or tissues.
DR   GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0008301; F:DNA binding, bending; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd21978; HMG-box_HMGB_rpt1; 1.
DR   CDD; cd21979; HMG-box_HMGB_rpt2; 1.
DR   Gene3D; 1.10.30.10; High mobility group box domain; 2.
DR   InterPro; IPR009071; HMG_box_dom.
DR   InterPro; IPR036910; HMG_box_dom_sf.
DR   PANTHER; PTHR48112:SF7; HIGH MOBILITY GROUP PROTEIN B4; 1.
DR   PANTHER; PTHR48112; HIGH MOBILITY GROUP PROTEIN DSP1; 1.
DR   Pfam; PF00505; HMG_box; 1.
DR   Pfam; PF09011; HMG_box_2; 1.
DR   PRINTS; PR00886; HIGHMOBLTY12.
DR   SMART; SM00398; HMG; 2.
DR   SUPFAM; SSF47095; HMG-box; 2.
DR   PROSITE; PS50118; HMG_BOX_2; 2.
DR   Genevisible; Q6P8W9; MM.
PE   1: Evidence at protein level;
KW   Chromosome; DNA-binding; Nucleus; Reference proteome; Repeat.
FT   CHAIN           1..181
FT                   /note="High mobility group protein B4"
FT                   /id="PRO_0000269181"
FT   DNA_BIND        9..79
FT                   /note="HMG box 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT   DNA_BIND        93..161
FT                   /note="HMG box 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT   REGION          80..100
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        66
FT                   /note="L -> H (in Ref. 1; BAB24141)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   181 AA;  21604 MW;  A41FDE8F08413DBA CRC64;
     MGEKDQLRPK VNVSSYIHFM LNFRNKFKEQ QPNTYLGFKE FSRKCSEKWR SISKHEKAKY
     EALAELDKAR YQQEMMNYIG KRRKRRKRDP KAPRKPPSSF LLFSRDHYAM LKQENPDWTV
     VQVAKAAGKM WSTTDEAEKK PYEQKAALMR AKYFEEQEAY RNQCQGRKGN FLESAKTSLK
     Q
//