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Protein

Pancreatic triacylglycerol lipase

Gene

Pnlip

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in fat metabolism. It preferentially splits the esters of long-chain fatty acids at positions 1 and 3, producing mainly 2-monoacylglycerol and free fatty acids, and shows considerably higher activity against insoluble emulsified substrates than against soluble ones (By similarity).By similarity

Catalytic activityi

Triacylglycerol + H2O = diacylglycerol + a carboxylate.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei169 – 1691NucleophileBy similarity
Active sitei193 – 1931Charge relay systemPROSITE-ProRule annotation
Metal bindingi204 – 2041Calcium; via carbonyl oxygenBy similarity
Metal bindingi207 – 2071Calcium; via carbonyl oxygenBy similarity
Metal bindingi209 – 2091CalciumBy similarity
Metal bindingi212 – 2121CalciumBy similarity
Active sitei280 – 2801Charge relay systemPROSITE-ProRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. triglyceride lipase activity Source: UniProtKB-EC

GO - Biological processi

  1. intestinal cholesterol absorption Source: MGI
  2. lipid catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_198569. Retinoid metabolism and transport.
REACT_204917. Digestion of dietary lipid.

Names & Taxonomyi

Protein namesi
Recommended name:
Pancreatic triacylglycerol lipase (EC:3.1.1.3)
Short name:
PL
Short name:
PTL
Short name:
Pancreatic lipase
Gene namesi
Name:Pnlip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:97722. Pnlip.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence AnalysisAdd
BLAST
Chaini17 – 465449Pancreatic triacylglycerol lipasePRO_0000401139Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi20 ↔ 26PROSITE-ProRule annotation
Disulfide bondi107 ↔ 118PROSITE-ProRule annotation
Disulfide bondi254 ↔ 278PROSITE-ProRule annotation
Disulfide bondi302 ↔ 313PROSITE-ProRule annotation
Disulfide bondi316 ↔ 321PROSITE-ProRule annotation
Disulfide bondi449 ↔ 465PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiQ6P8U6.
PaxDbiQ6P8U6.
PRIDEiQ6P8U6.

PTM databases

PhosphoSiteiQ6P8U6.

Expressioni

Tissue specificityi

Pancreas.1 Publication

Inductioni

By colipase/CLPS in the presence of bile salts.

Gene expression databases

BgeeiQ6P8U6.
GenevestigatoriQ6P8U6.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000056377.

Structurei

3D structure databases

ProteinModelPortaliQ6P8U6.
SMRiQ6P8U6. Positions 18-465.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini355 – 465111PLATPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.Curated
Contains 1 PLAT domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG40923.
GeneTreeiENSGT00760000119069.
HOGENOMiHOG000038552.
HOVERGENiHBG003243.
InParanoidiQ6P8U6.
KOiK14073.
OMAiAGKEVCF.
OrthoDBiEOG7KSX8B.
PhylomeDBiQ6P8U6.
TreeFamiTF324997.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR016272. Lipase_LIPH.
IPR008976. Lipase_LipOase.
IPR013818. Lipase_N.
IPR002331. Lipase_panc.
IPR001024. PLAT/LH2_dom.
IPR000734. TAG_lipase.
[Graphical view]
PANTHERiPTHR11610. PTHR11610. 1 hit.
PfamiPF00151. Lipase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PIRSFiPIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
PRINTSiPR00823. PANCLIPASE.
PR00821. TAGLIPASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF49723. SSF49723. 1 hit.
SSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6P8U6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLMLWTFAVL LGAVAGREVC FDKLGCFSDD APWSGTLDRP LKALPWSPAQ
60 70 80 90 100
INTRFLLYTN ENPDNYQLIT SDASNIRNSN FRTNRKTRII IHGFIDKGEE
110 120 130 140 150
NWLSDMCKNM FRVESVNCIC VDWKGGSRTT YTQATQNVRV VGAEVALLVN
160 170 180 190 200
VLQSDLGYSL NNVHLIGHSL GSHIAGEAGK RTFGAIGRIT GLDPAEPYFQ
210 220 230 240 250
GTPEEVRLDP TDAQFVDAIH TDAGPIIPNL GFGMSQTVGH LDFFPNGGIE
260 270 280 290 300
MPGCQKNILS QIVDIDGIWE GTRNFAACNH LRSYKFYTDS IVNPTGFAGF
310 320 330 340 350
SCSSYSLFTA NKCFPCGSGG CPQMGHYADR YPGKTSRLYQ TFYLNTGDKS
360 370 380 390 400
NFARWRYQVT VTLSGQKVTG HILVSLFGNG GNSKQYEVFK GSLQPGTSHV
410 420 430 440 450
NEFDSDVDVG DLQKVKFIWY NNVINPTLPK VGASRITVER NDGRVFNFCS
460
QETVREDVLL TLSPC
Length:465
Mass (Da):51,428
Last modified:July 5, 2004 - v1
Checksum:i5CD39E8ABDF43A64
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY387690 mRNA. Translation: AAQ90020.1.
CH466585 Genomic DNA. Translation: EDL01803.1.
BC061061 mRNA. Translation: AAH61061.1.
CCDSiCCDS29930.1.
RefSeqiNP_081201.2. NM_026925.3.
UniGeneiMm.20407.

Genome annotation databases

EnsembliENSMUST00000057270; ENSMUSP00000056377; ENSMUSG00000046008.
GeneIDi69060.
KEGGimmu:69060.
UCSCiuc008iaq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY387690 mRNA. Translation: AAQ90020.1.
CH466585 Genomic DNA. Translation: EDL01803.1.
BC061061 mRNA. Translation: AAH61061.1.
CCDSiCCDS29930.1.
RefSeqiNP_081201.2. NM_026925.3.
UniGeneiMm.20407.

3D structure databases

ProteinModelPortaliQ6P8U6.
SMRiQ6P8U6. Positions 18-465.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000056377.

PTM databases

PhosphoSiteiQ6P8U6.

Proteomic databases

MaxQBiQ6P8U6.
PaxDbiQ6P8U6.
PRIDEiQ6P8U6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000057270; ENSMUSP00000056377; ENSMUSG00000046008.
GeneIDi69060.
KEGGimmu:69060.
UCSCiuc008iaq.1. mouse.

Organism-specific databases

CTDi5406.
MGIiMGI:97722. Pnlip.

Phylogenomic databases

eggNOGiNOG40923.
GeneTreeiENSGT00760000119069.
HOGENOMiHOG000038552.
HOVERGENiHBG003243.
InParanoidiQ6P8U6.
KOiK14073.
OMAiAGKEVCF.
OrthoDBiEOG7KSX8B.
PhylomeDBiQ6P8U6.
TreeFamiTF324997.

Enzyme and pathway databases

ReactomeiREACT_198569. Retinoid metabolism and transport.
REACT_204917. Digestion of dietary lipid.

Miscellaneous databases

ChiTaRSiPnlip. mouse.
NextBioi328500.
PROiQ6P8U6.
SOURCEiSearch...

Gene expression databases

BgeeiQ6P8U6.
GenevestigatoriQ6P8U6.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR016272. Lipase_LIPH.
IPR008976. Lipase_LipOase.
IPR013818. Lipase_N.
IPR002331. Lipase_panc.
IPR001024. PLAT/LH2_dom.
IPR000734. TAG_lipase.
[Graphical view]
PANTHERiPTHR11610. PTHR11610. 1 hit.
PfamiPF00151. Lipase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PIRSFiPIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
PRINTSiPR00823. PANCLIPASE.
PR00821. TAGLIPASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF49723. SSF49723. 1 hit.
SSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Feeding activates protein synthesis in mouse pancreas at the translational level without increase in mRNA."
    Sans M.D., Lee S.H., D'Alecy L.G., Williams J.A.
    Am. J. Physiol. 287:G667-G675(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ICR.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  4. "Hydrolysis of retinyl esters by pancreatic triglyceride lipase."
    van Bennekum A.M., Fisher E.A., Blaner W.S., Harrison E.H.
    Biochemistry 39:4900-4906(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, TISSUE SPECIFICITY.

Entry informationi

Entry nameiLIPP_MOUSE
AccessioniPrimary (citable) accession number: Q6P8U6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: July 5, 2004
Last modified: January 7, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.