Reviewed, UniProtKB/Swiss-Prot Q6P8J7 (KCRS_MOUSE)

Names and origin

Protein names

Recommended name:
    Creatine kinase, sarcomeric mitochondrial
    EC=2.7.3.2
Alternative name(s):
    S-MtCK

Gene names

Name:

Ckmt2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa By similarity.
Catalytic activity	ATP + creatine = ADP + phosphocreatine.
Subunit structure	Exists as an octamer composed of four CKMT2 homodimers By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side By similarity.
Miscellaneous	Mitochondrial creatine kinase binds cardiolipin.
Sequence similarities	Belongs to the ATP:guanido phosphotransferase family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	phosphocreatine metabolic process Traceable author statement. Source: MGI
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW creatine kinase activity Traceable author statement. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 39	39	Mitochondrion
	Chain	40 – 419	380	Creatine kinase, sarcomeric mitochondrial		PRO_0000016595
Regions
	Nucleotide binding	162 – 166	5	ATP By similarity
	Nucleotide binding	354 – 359	6	ATP By similarity
	Region	40 – 64	25	Cardiolipin-binding By similarity
Sites
	Binding site	225	1	ATP By similarity
	Binding site	270	1	ATP By similarity
	Binding site	326	1	ATP By similarity
	Binding site	369	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6P8J7-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: E9083E4F689903EF	FASTA	419	47,473
	10 20 30 40 50 60 MASAFSKLLT GRNASLLFTT LGTSALTTGY LLNRQKVSAD AREQHKLFPP SADYPDLRKH 70 80 90 100 110 120 NNCMAECLTP TIYAKLRNKM TPSGYTLDQC IQTGVDNPGH PFIKTVGMVA GDEESYEVFA 130 140 150 160 170 180 DLFDPVIKLR HNGYDPRVMK HPTDLDASKI THGQFDERYV LSSRVRTGRS IRGLSLPPAC 190 200 210 220 230 240 SRAERREVEN VAITALEGLK GDLAGRYYKL SEMTEQDQQR LIDDHFLFDK PVSPLLTCAG 250 260 270 280 290 300 MARDWPDARG IWHNYDKTFL IWINEEDHTR VISMEKGGNM KRVFERFCRG LKEVERLIQE 310 320 330 340 350 360 RGWEFMWNER LGYILTCPSN LGTGLRAGVH VRIPKLSKDP RFSKILENLR LQKRGTGGVD 370 380 390 400 410 TAAVADVYDI SNIDRIGRSE VELVQIVIDG VNYLVDCEKK LERGQDIKVP PPLPQFGRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart and Lung.
[2]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 311-326, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC061221 mRNA. Translation: AAH61221.1.
IPI	IPI00120076.
RefSeq	NP_940807.1.
UniGene	Mm.316438
3D structure databases
SMR	Q6P8J7. Positions 47-413.
ModBase	Search...
Proteomic databases
PRIDE	Q6P8J7.
Genome annotation databases
Ensembl	ENSMUSG00000021622. Mus musculus. [Contig view]
GeneID	76722.
KEGG	mmu:76722.
Organism-specific databases
MGI	MGI:1923972. Ckmt2.
Phylogenomic databases
HOGENOM	Q6P8J7.
HOVERGEN	Q6P8J7.
OMA	Q6P8J7. TPSIYAK.
Enzyme and pathway databases
BRENDA	2.7.3.2. 244.
Gene expression databases
ArrayExpress	Q6P8J7.
Bgee	Q6P8J7.
CleanEx	MM_CKMT2.
GermOnline	ENSMUSG00000021622. Mus musculus.
Family and domain databases
InterPro	IPR000749. ATP-guanido_PTrfase. IPR014746. Gln_synth/guanido_kin_cat. [Graphical view]
Gene3D	G3DSA:1.10.135.10. ATP-gua_Ptrans. 1 hit. G3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit.
PANTHER	PTHR11547. ATP-gua_Ptrans. 1 hit.
Pfam	PF00217. ATP-gua_Ptrans. 1 hit. PF02807. ATP-gua_PtransN. 1 hit. [Graphical view]
PROSITE	PS00112. GUANIDO_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	345699.
SOURCE	Search...

Entry information

Entry name

KCRS_MOUSE

Accession

Primary (citable) accession number: Q6P8J7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)