Reviewed, UniProtKB/Swiss-Prot Q6P8J2 (SAT2_MOUSE)

Names and origin

Protein names

Recommended name:
    Diamine acetyltransferase 2
    EC=2.3.1.57
Alternative name(s):
    Spermidine/spermine N(1)-acetyltransferase 2
    Polyamine N-acetyltransferase 2

Gene names

Name:	Sat2
Synonyms:	Ssat2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine > spermidine = spermine >> N1acetylspermine = putrescine By similarity.
Catalytic activity	Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine.
Pathway	Amine and polyamine degradation; putrescine degradation; N-acetylputrescine from putrescine: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Note: Intracellular organelles By similarity.
Sequence similarities	Belongs to the acetyltransferase family. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Molecular function	Acyltransferase Transferase
PTM	Acetylation
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	diamine N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 170	170	Diamine acetyltransferase 2		PRO_0000074599
Regions
	Domain	4 – 166	163	N-acetyltransferase
	Region	27 – 28	2	Substrate binding By similarity
	Region	94 – 96	3	Acetyl-CoA binding By similarity
	Region	102 – 107	6	Acetyl-CoA binding By similarity
	Region	133 – 135	3	Acetyl-CoA binding By similarity
Sites
	Binding site	92	1	Substrate; via carbonyl oxygen By similarity
	Binding site	140	1	Acetyl-CoA By similarity
	Binding site	152	1	Substrate By similarity
Amino acid modifications
	Modified residue	29	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6P8J2-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 5D82F98DAD0619AA	FASTA	170	19,305
	10 20 30 40 50 60 MASTRIREAR ESDCGDIMRM IRELAEFEKL SHQVKISEEA LRADGFGENP FFHCLVAEII 70 80 90 100 110 120 PAPGESQGSL VVGYGLYYFI YSTWTGRNVY LEDIYVMPQY RGQGIGTKII KKVAEVALNK 130 140 150 160 170 GCSQFRLAVL DWNKKAVNLY KFLGAQDLTE SEGWLSFRFE GEAMRELAGR

References

[1]

"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.

[2]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL603707, AL731687 Genomic DNA. Translation: CAI51952.1. BC061227 mRNA. Translation: AAH61227.1.
IPI	IPI00347376.
RefSeq	NP_081267.1.
UniGene	Mm.340410
3D structure databases
SMR	Q6P8J2. Positions 2-170.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6P8J2.
Proteomic databases
PRIDE	Q6P8J2.
Genome annotation databases
Ensembl	ENSMUST00000092969; ENSMUSP00000090647; ENSMUSG00000069835; Mus musculus. [Genome view] ENSMUST00000108656; ENSMUSP00000104296; ENSMUSG00000069835; Mus musculus. [Genome view]
GeneID	69215.
KEGG	mmu:69215.
UCSC	uc007jqq.1. mouse.
Organism-specific databases
CTD	69215.
MGI	MGI:1916465. Sat2.
Phylogenomic databases
HOGENOM	Q6P8J2.
HOVERGEN	Q6P8J2.
OMA	ITAMIRE.
Enzyme and pathway databases
BRENDA	2.3.1.57. 244.
Gene expression databases
ArrayExpress	Q6P8J2.
Bgee	Q6P8J2.
CleanEx	MM_SAT2.
Genevestigator	Q6P8J2.
GermOnline	ENSMUSG00000069835. Mus musculus.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000182. GCN5-rel_AcTrfase. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	328895.
SOURCE	Search...

Entry information

Entry name

SAT2_MOUSE

Accession

Primary (citable) accession number: Q6P8J2
Secondary accession number(s): Q5F299

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)