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Q6P8H8 (ALG8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase

EC=2.4.1.265
Alternative name(s):
Asparagine-linked glycosylation protein 8 homolog
Dol-P-Glc:Glc(1)Man(9)GlcNAc(2)-PP-dolichyl alpha-1,3-glucosyltransferase
Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase
Gene names
Name:Alg8
Synonyms:Gm1089
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length526 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc1Man9GlcNAc(2)-PP-Dol By similarity.

Catalytic activity

Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the ALG6/ALG8 glucosyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 526526Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase
PRO_0000174163

Regions

Transmembrane9 – 2921Helical; Potential
Transmembrane108 – 12821Helical; Potential
Transmembrane143 – 16321Helical; Potential
Transmembrane188 – 20821Helical; Potential
Transmembrane238 – 25821Helical; Potential
Transmembrane334 – 35421Helical; Potential
Transmembrane361 – 38020Helical; Potential
Transmembrane400 – 42223Helical; Potential
Transmembrane427 – 44923Helical; Potential
Transmembrane461 – 48121Helical; Potential
Transmembrane487 – 50721Helical; Potential

Experimental info

Sequence conflict91A → G in AAH61244. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q6P8H8 [UniParc].

Last modified June 28, 2011. Version 2.
Checksum: 5AB213946EF09D11

FASTA52659,535
        10         20         30         40         50         60 
MAASGSATAG GHWFSALALG VTLLKCLLIP TYHSTDFEVH RNWLAITHSL PISQWYYEAT 

        70         80         90        100        110        120 
SEWTLDYPPF FAWFEYALSH IAKYFDQEML NIHNLNYYSS RTLLFQRFSV ILTDALFVYA 

       130        140        150        160        170        180 
VHECCKCIDG KRTGKDLTEK PKFILSVLLL WNFGLLIVDH IHFQYNGFLS GLLLLSIARL 

       190        200        210        220        230        240 
FQKRHIEGAL LFAVLLHLKH IYLYVAPAYG VYLLRSYCFT ASKPDGSVRW SSFSVVRVTS 

       250        260        270        280        290        300 
LGLIVFLVSA LSLGPFLALN QLPQVFSRLF PFKRGLCHAY WAPNFWALYN ALDKVLSVIG 

       310        320        330        340        350        360 
LKLKLLDPSQ IPRASMTSGL VQQFQHTVLP SVSPLATLIC TLIAILPSVF CLWFKPQGPR 

       370        380        390        400        410        420 
GFLRCLVLCA LSSFMFGWHV HEKAILLAIL PMSLLSVEKA GDATVFLILA TTGHYSLFPL 

       430        440        450        460        470        480 
LFTAPELPIK ILLMLLFTVY SISSLKTLFR KEKPLFNWME TVYLLGLGPL EVCCEFLLPF 

       490        500        510        520 
TSWKLKYPFI PLLLTSVYCA VGITYAWTRL YASVLTGSLV SKTKKH 

« Hide

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pituitary.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK166755 mRNA. Translation: BAE38996.1.
AK166897 mRNA. Translation: BAE39100.1.
AC155933 Genomic DNA. No translation available.
CH466531 Genomic DNA. Translation: EDL16291.1.
BC061244 mRNA. Translation: AAH61244.1.
RefSeqNP_950200.2. NM_199035.2.
UniGeneMm.279683.
Mm.407368.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT57. Glycosyltransferase Family 57.

PTM databases

PhosphoSiteQ6P8H8.

Proteomic databases

PaxDbQ6P8H8.
PRIDEQ6P8H8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000098300; ENSMUSP00000095901; ENSMUSG00000035704.
GeneID381903.
KEGGmmu:381903.
UCSCuc009ijc.2. mouse.

Organism-specific databases

CTD79053.
MGIMGI:2141959. Alg8.

Phylogenomic databases

eggNOGNOG277067.
GeneTreeENSGT00550000074967.
HOGENOMHOG000168174.
HOVERGENHBG024332.
InParanoidQ3TKP5.
KOK03849.
OMAALTLCGY.
OrthoDBEOG7NW691.
TreeFamTF315002.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

BgeeQ6P8H8.
GenevestigatorQ6P8H8.

Family and domain databases

InterProIPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
PANTHERPTHR12413. PTHR12413. 1 hit.
PfamPF03155. Alg6_Alg8. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSALG8. mouse.
NextBio402733.
PROQ6P8H8.
SOURCESearch...

Entry information

Entry nameALG8_MOUSE
AccessionPrimary (citable) accession number: Q6P8H8
Secondary accession number(s): E9Q3H5, Q3TKP5
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: June 28, 2011
Last modified: April 16, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot