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Q6P8H8

- ALG8_MOUSE

UniProt

Q6P8H8 - ALG8_MOUSE

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Protein
Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase
Gene
Alg8, Gm1089
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc1Man9GlcNAc(2)-PP-Dol By similarity.

Catalytic activityi

Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathwayi

GO - Molecular functioni

  1. alpha-1,3-mannosyltransferase activity Source: UniProtKB

GO - Biological processi

  1. mannosylation Source: GOC
  2. protein N-linked glycosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT57. Glycosyltransferase Family 57.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC:2.4.1.265)
Alternative name(s):
Asparagine-linked glycosylation protein 8 homolog
Dol-P-Glc:Glc(1)Man(9)GlcNAc(2)-PP-dolichyl alpha-1,3-glucosyltransferase
Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase
Gene namesi
Name:Alg8
Synonyms:Gm1089
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:2141959. Alg8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei9 – 2921Helical; Reviewed prediction
Add
BLAST
Transmembranei108 – 12821Helical; Reviewed prediction
Add
BLAST
Transmembranei143 – 16321Helical; Reviewed prediction
Add
BLAST
Transmembranei188 – 20821Helical; Reviewed prediction
Add
BLAST
Transmembranei238 – 25821Helical; Reviewed prediction
Add
BLAST
Transmembranei334 – 35421Helical; Reviewed prediction
Add
BLAST
Transmembranei361 – 38020Helical; Reviewed prediction
Add
BLAST
Transmembranei400 – 42223Helical; Reviewed prediction
Add
BLAST
Transmembranei427 – 44923Helical; Reviewed prediction
Add
BLAST
Transmembranei461 – 48121Helical; Reviewed prediction
Add
BLAST
Transmembranei487 – 50721Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 526526Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase
PRO_0000174163Add
BLAST

Proteomic databases

MaxQBiQ6P8H8.
PaxDbiQ6P8H8.
PRIDEiQ6P8H8.

PTM databases

PhosphoSiteiQ6P8H8.

Expressioni

Gene expression databases

BgeeiQ6P8H8.
GenevestigatoriQ6P8H8.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG277067.
GeneTreeiENSGT00550000074967.
HOGENOMiHOG000168174.
HOVERGENiHBG024332.
InParanoidiQ3TKP5.
KOiK03849.
OMAiALTLCGY.
OrthoDBiEOG7NW691.
TreeFamiTF315002.

Family and domain databases

InterProiIPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
PANTHERiPTHR12413. PTHR12413. 1 hit.
PfamiPF03155. Alg6_Alg8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6P8H8-1 [UniParc]FASTAAdd to Basket

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MAASGSATAG GHWFSALALG VTLLKCLLIP TYHSTDFEVH RNWLAITHSL    50
PISQWYYEAT SEWTLDYPPF FAWFEYALSH IAKYFDQEML NIHNLNYYSS 100
RTLLFQRFSV ILTDALFVYA VHECCKCIDG KRTGKDLTEK PKFILSVLLL 150
WNFGLLIVDH IHFQYNGFLS GLLLLSIARL FQKRHIEGAL LFAVLLHLKH 200
IYLYVAPAYG VYLLRSYCFT ASKPDGSVRW SSFSVVRVTS LGLIVFLVSA 250
LSLGPFLALN QLPQVFSRLF PFKRGLCHAY WAPNFWALYN ALDKVLSVIG 300
LKLKLLDPSQ IPRASMTSGL VQQFQHTVLP SVSPLATLIC TLIAILPSVF 350
CLWFKPQGPR GFLRCLVLCA LSSFMFGWHV HEKAILLAIL PMSLLSVEKA 400
GDATVFLILA TTGHYSLFPL LFTAPELPIK ILLMLLFTVY SISSLKTLFR 450
KEKPLFNWME TVYLLGLGPL EVCCEFLLPF TSWKLKYPFI PLLLTSVYCA 500
VGITYAWTRL YASVLTGSLV SKTKKH 526
Length:526
Mass (Da):59,535
Last modified:June 28, 2011 - v2
Checksum:i5AB213946EF09D11
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91A → G in AAH61244. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK166755 mRNA. Translation: BAE38996.1.
AK166897 mRNA. Translation: BAE39100.1.
AC155933 Genomic DNA. No translation available.
CH466531 Genomic DNA. Translation: EDL16291.1.
BC061244 mRNA. Translation: AAH61244.1.
CCDSiCCDS52313.1.
RefSeqiNP_950200.2. NM_199035.2.
UniGeneiMm.279683.
Mm.407368.

Genome annotation databases

EnsembliENSMUST00000098300; ENSMUSP00000095901; ENSMUSG00000035704.
GeneIDi381903.
KEGGimmu:381903.
UCSCiuc009ijc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK166755 mRNA. Translation: BAE38996.1 .
AK166897 mRNA. Translation: BAE39100.1 .
AC155933 Genomic DNA. No translation available.
CH466531 Genomic DNA. Translation: EDL16291.1 .
BC061244 mRNA. Translation: AAH61244.1 .
CCDSi CCDS52313.1.
RefSeqi NP_950200.2. NM_199035.2.
UniGenei Mm.279683.
Mm.407368.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT57. Glycosyltransferase Family 57.

PTM databases

PhosphoSitei Q6P8H8.

Proteomic databases

MaxQBi Q6P8H8.
PaxDbi Q6P8H8.
PRIDEi Q6P8H8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000098300 ; ENSMUSP00000095901 ; ENSMUSG00000035704 .
GeneIDi 381903.
KEGGi mmu:381903.
UCSCi uc009ijc.2. mouse.

Organism-specific databases

CTDi 79053.
MGIi MGI:2141959. Alg8.

Phylogenomic databases

eggNOGi NOG277067.
GeneTreei ENSGT00550000074967.
HOGENOMi HOG000168174.
HOVERGENi HBG024332.
InParanoidi Q3TKP5.
KOi K03849.
OMAi ALTLCGY.
OrthoDBi EOG7NW691.
TreeFami TF315002.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Miscellaneous databases

ChiTaRSi ALG8. mouse.
NextBioi 402733.
PROi Q6P8H8.
SOURCEi Search...

Gene expression databases

Bgeei Q6P8H8.
Genevestigatori Q6P8H8.

Family and domain databases

InterProi IPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view ]
PANTHERi PTHR12413. PTHR12413. 1 hit.
Pfami PF03155. Alg6_Alg8. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.

Entry informationi

Entry nameiALG8_MOUSE
AccessioniPrimary (citable) accession number: Q6P8H8
Secondary accession number(s): E9Q3H5, Q3TKP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: June 28, 2011
Last modified: July 9, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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