Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q6P8H8

- ALG8_MOUSE

UniProt

Q6P8H8 - ALG8_MOUSE

Protein

Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase

Gene

Alg8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 2 (28 Jun 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc1Man9GlcNAc(2)-PP-Dol By similarity.By similarity

    Catalytic activityi

    Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

    Pathwayi

    GO - Molecular functioni

    1. alpha-1,3-mannosyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. mannosylation Source: GOC
    2. protein N-linked glycosylation Source: UniProtKB

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT57. Glycosyltransferase Family 57.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC:2.4.1.265)
    Alternative name(s):
    Asparagine-linked glycosylation protein 8 homolog
    Dol-P-Glc:Glc(1)Man(9)GlcNAc(2)-PP-dolichyl alpha-1,3-glucosyltransferase
    Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase
    Gene namesi
    Name:Alg8
    Synonyms:Gm1089
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:2141959. Alg8.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 526526Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferasePRO_0000174163Add
    BLAST

    Proteomic databases

    MaxQBiQ6P8H8.
    PaxDbiQ6P8H8.
    PRIDEiQ6P8H8.

    PTM databases

    PhosphoSiteiQ6P8H8.

    Expressioni

    Gene expression databases

    BgeeiQ6P8H8.
    GenevestigatoriQ6P8H8.

    Structurei

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei9 – 2921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei108 – 12821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei143 – 16321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei188 – 20821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei238 – 25821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei334 – 35421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei361 – 38020HelicalSequence AnalysisAdd
    BLAST
    Transmembranei400 – 42223HelicalSequence AnalysisAdd
    BLAST
    Transmembranei427 – 44923HelicalSequence AnalysisAdd
    BLAST
    Transmembranei461 – 48121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei487 – 50721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG277067.
    GeneTreeiENSGT00550000074967.
    HOGENOMiHOG000168174.
    HOVERGENiHBG024332.
    InParanoidiQ3TKP5.
    KOiK03849.
    OMAiALTLCGY.
    OrthoDBiEOG7NW691.
    TreeFamiTF315002.

    Family and domain databases

    InterProiIPR004856. Glyco_trans_ALG6/ALG8.
    [Graphical view]
    PANTHERiPTHR12413. PTHR12413. 1 hit.
    PfamiPF03155. Alg6_Alg8. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6P8H8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAASGSATAG GHWFSALALG VTLLKCLLIP TYHSTDFEVH RNWLAITHSL    50
    PISQWYYEAT SEWTLDYPPF FAWFEYALSH IAKYFDQEML NIHNLNYYSS 100
    RTLLFQRFSV ILTDALFVYA VHECCKCIDG KRTGKDLTEK PKFILSVLLL 150
    WNFGLLIVDH IHFQYNGFLS GLLLLSIARL FQKRHIEGAL LFAVLLHLKH 200
    IYLYVAPAYG VYLLRSYCFT ASKPDGSVRW SSFSVVRVTS LGLIVFLVSA 250
    LSLGPFLALN QLPQVFSRLF PFKRGLCHAY WAPNFWALYN ALDKVLSVIG 300
    LKLKLLDPSQ IPRASMTSGL VQQFQHTVLP SVSPLATLIC TLIAILPSVF 350
    CLWFKPQGPR GFLRCLVLCA LSSFMFGWHV HEKAILLAIL PMSLLSVEKA 400
    GDATVFLILA TTGHYSLFPL LFTAPELPIK ILLMLLFTVY SISSLKTLFR 450
    KEKPLFNWME TVYLLGLGPL EVCCEFLLPF TSWKLKYPFI PLLLTSVYCA 500
    VGITYAWTRL YASVLTGSLV SKTKKH 526
    Length:526
    Mass (Da):59,535
    Last modified:June 28, 2011 - v2
    Checksum:i5AB213946EF09D11
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti9 – 91A → G in AAH61244. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK166755 mRNA. Translation: BAE38996.1.
    AK166897 mRNA. Translation: BAE39100.1.
    AC155933 Genomic DNA. No translation available.
    CH466531 Genomic DNA. Translation: EDL16291.1.
    BC061244 mRNA. Translation: AAH61244.1.
    CCDSiCCDS52313.1.
    RefSeqiNP_950200.2. NM_199035.2.
    UniGeneiMm.279683.
    Mm.407368.

    Genome annotation databases

    EnsembliENSMUST00000098300; ENSMUSP00000095901; ENSMUSG00000035704.
    GeneIDi381903.
    KEGGimmu:381903.
    UCSCiuc009ijc.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK166755 mRNA. Translation: BAE38996.1 .
    AK166897 mRNA. Translation: BAE39100.1 .
    AC155933 Genomic DNA. No translation available.
    CH466531 Genomic DNA. Translation: EDL16291.1 .
    BC061244 mRNA. Translation: AAH61244.1 .
    CCDSi CCDS52313.1.
    RefSeqi NP_950200.2. NM_199035.2.
    UniGenei Mm.279683.
    Mm.407368.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT57. Glycosyltransferase Family 57.

    PTM databases

    PhosphoSitei Q6P8H8.

    Proteomic databases

    MaxQBi Q6P8H8.
    PaxDbi Q6P8H8.
    PRIDEi Q6P8H8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000098300 ; ENSMUSP00000095901 ; ENSMUSG00000035704 .
    GeneIDi 381903.
    KEGGi mmu:381903.
    UCSCi uc009ijc.2. mouse.

    Organism-specific databases

    CTDi 79053.
    MGIi MGI:2141959. Alg8.

    Phylogenomic databases

    eggNOGi NOG277067.
    GeneTreei ENSGT00550000074967.
    HOGENOMi HOG000168174.
    HOVERGENi HBG024332.
    InParanoidi Q3TKP5.
    KOi K03849.
    OMAi ALTLCGY.
    OrthoDBi EOG7NW691.
    TreeFami TF315002.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    ChiTaRSi ALG8. mouse.
    NextBioi 402733.
    PROi Q6P8H8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q6P8H8.
    Genevestigatori Q6P8H8.

    Family and domain databases

    InterProi IPR004856. Glyco_trans_ALG6/ALG8.
    [Graphical view ]
    PANTHERi PTHR12413. PTHR12413. 1 hit.
    Pfami PF03155. Alg6_Alg8. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pituitary.

    Entry informationi

    Entry nameiALG8_MOUSE
    AccessioniPrimary (citable) accession number: Q6P8H8
    Secondary accession number(s): E9Q3H5, Q3TKP5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 78 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3