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Protein

Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase

Gene

Alg8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc1Man9GlcNAc(2)-PP-Dol (By similarity).By similarity

Catalytic activityi

Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathwayi

GO - Molecular functioni

  1. alpha-1,3-mannosyltransferase activity Source: UniProtKB
  2. dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity Source: GO_Central

GO - Biological processi

  1. mannosylation Source: MGI
  2. oligosaccharide-lipid intermediate biosynthetic process Source: GO_Central
  3. protein N-linked glycosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_320215. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT57. Glycosyltransferase Family 57.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC:2.4.1.265)
Alternative name(s):
Asparagine-linked glycosylation protein 8 homolog
Dol-P-Glc:Glc(1)Man(9)GlcNAc(2)-PP-dolichyl alpha-1,3-glucosyltransferase
Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase
Gene namesi
Name:Alg8
Synonyms:Gm1089
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2141959. Alg8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei9 – 2921HelicalSequence AnalysisAdd
BLAST
Transmembranei108 – 12821HelicalSequence AnalysisAdd
BLAST
Transmembranei143 – 16321HelicalSequence AnalysisAdd
BLAST
Transmembranei188 – 20821HelicalSequence AnalysisAdd
BLAST
Transmembranei238 – 25821HelicalSequence AnalysisAdd
BLAST
Transmembranei334 – 35421HelicalSequence AnalysisAdd
BLAST
Transmembranei361 – 38020HelicalSequence AnalysisAdd
BLAST
Transmembranei400 – 42223HelicalSequence AnalysisAdd
BLAST
Transmembranei427 – 44923HelicalSequence AnalysisAdd
BLAST
Transmembranei461 – 48121HelicalSequence AnalysisAdd
BLAST
Transmembranei487 – 50721HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: GO_Central
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 526526Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferasePRO_0000174163Add
BLAST

Proteomic databases

MaxQBiQ6P8H8.
PaxDbiQ6P8H8.
PRIDEiQ6P8H8.

PTM databases

PhosphoSiteiQ6P8H8.

Expressioni

Gene expression databases

BgeeiQ6P8H8.
GenevestigatoriQ6P8H8.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG277067.
GeneTreeiENSGT00550000074967.
HOGENOMiHOG000168174.
HOVERGENiHBG024332.
InParanoidiQ6P8H8.
KOiK03849.
OMAiWKLKYPF.
OrthoDBiEOG7NW691.
TreeFamiTF315002.

Family and domain databases

InterProiIPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
PANTHERiPTHR12413. PTHR12413. 1 hit.
PfamiPF03155. Alg6_Alg8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6P8H8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASGSATAG GHWFSALALG VTLLKCLLIP TYHSTDFEVH RNWLAITHSL
60 70 80 90 100
PISQWYYEAT SEWTLDYPPF FAWFEYALSH IAKYFDQEML NIHNLNYYSS
110 120 130 140 150
RTLLFQRFSV ILTDALFVYA VHECCKCIDG KRTGKDLTEK PKFILSVLLL
160 170 180 190 200
WNFGLLIVDH IHFQYNGFLS GLLLLSIARL FQKRHIEGAL LFAVLLHLKH
210 220 230 240 250
IYLYVAPAYG VYLLRSYCFT ASKPDGSVRW SSFSVVRVTS LGLIVFLVSA
260 270 280 290 300
LSLGPFLALN QLPQVFSRLF PFKRGLCHAY WAPNFWALYN ALDKVLSVIG
310 320 330 340 350
LKLKLLDPSQ IPRASMTSGL VQQFQHTVLP SVSPLATLIC TLIAILPSVF
360 370 380 390 400
CLWFKPQGPR GFLRCLVLCA LSSFMFGWHV HEKAILLAIL PMSLLSVEKA
410 420 430 440 450
GDATVFLILA TTGHYSLFPL LFTAPELPIK ILLMLLFTVY SISSLKTLFR
460 470 480 490 500
KEKPLFNWME TVYLLGLGPL EVCCEFLLPF TSWKLKYPFI PLLLTSVYCA
510 520
VGITYAWTRL YASVLTGSLV SKTKKH
Length:526
Mass (Da):59,535
Last modified:June 27, 2011 - v2
Checksum:i5AB213946EF09D11
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91A → G in AAH61244 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK166755 mRNA. Translation: BAE38996.1.
AK166897 mRNA. Translation: BAE39100.1.
AC155933 Genomic DNA. No translation available.
CH466531 Genomic DNA. Translation: EDL16291.1.
BC061244 mRNA. Translation: AAH61244.1.
CCDSiCCDS52313.1.
RefSeqiNP_950200.2. NM_199035.2.
UniGeneiMm.279683.
Mm.407368.

Genome annotation databases

EnsembliENSMUST00000098300; ENSMUSP00000095901; ENSMUSG00000035704.
GeneIDi381903.
KEGGimmu:381903.
UCSCiuc009ijc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK166755 mRNA. Translation: BAE38996.1.
AK166897 mRNA. Translation: BAE39100.1.
AC155933 Genomic DNA. No translation available.
CH466531 Genomic DNA. Translation: EDL16291.1.
BC061244 mRNA. Translation: AAH61244.1.
CCDSiCCDS52313.1.
RefSeqiNP_950200.2. NM_199035.2.
UniGeneiMm.279683.
Mm.407368.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT57. Glycosyltransferase Family 57.

PTM databases

PhosphoSiteiQ6P8H8.

Proteomic databases

MaxQBiQ6P8H8.
PaxDbiQ6P8H8.
PRIDEiQ6P8H8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000098300; ENSMUSP00000095901; ENSMUSG00000035704.
GeneIDi381903.
KEGGimmu:381903.
UCSCiuc009ijc.2. mouse.

Organism-specific databases

CTDi79053.
MGIiMGI:2141959. Alg8.

Phylogenomic databases

eggNOGiNOG277067.
GeneTreeiENSGT00550000074967.
HOGENOMiHOG000168174.
HOVERGENiHBG024332.
InParanoidiQ6P8H8.
KOiK03849.
OMAiWKLKYPF.
OrthoDBiEOG7NW691.
TreeFamiTF315002.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_320215. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

ChiTaRSiAlg8. mouse.
NextBioi402733.
PROiQ6P8H8.
SOURCEiSearch...

Gene expression databases

BgeeiQ6P8H8.
GenevestigatoriQ6P8H8.

Family and domain databases

InterProiIPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
PANTHERiPTHR12413. PTHR12413. 1 hit.
PfamiPF03155. Alg6_Alg8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.

Entry informationi

Entry nameiALG8_MOUSE
AccessioniPrimary (citable) accession number: Q6P8H8
Secondary accession number(s): E9Q3H5, Q3TKP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 12, 2005
Last sequence update: June 27, 2011
Last modified: March 31, 2015
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.