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Q6P7W5

- SEN2_MOUSE

UniProt

Q6P7W5 - SEN2_MOUSE

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Protein

tRNA-splicing endonuclease subunit Sen2

Gene

Tsen2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei364 – 3641By similarity
Active sitei372 – 3721By similarity
Active sitei411 – 4111By similarity

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: InterPro

GO - Biological processi

  1. mRNA processing Source: UniProtKB-KW
  2. tRNA splicing, via endonucleolytic cleavage and ligation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

mRNA processing, tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease subunit Sen2 (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease Sen2
Gene namesi
Name:Tsen2
Synonyms:Sen2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:2141599. Tsen2.

Subcellular locationi

Nucleus By similarity. Nucleusnucleolus By similarity
Note: May be transiently localized in the nucleolus.By similarity

GO - Cellular componenti

  1. centrosome Source: Ensembl
  2. cytoplasm Source: Ensembl
  3. tRNA-intron endonuclease complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 460460tRNA-splicing endonuclease subunit Sen2PRO_0000109453Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei403 – 4031Phosphoserine1 Publication
Modified residuei406 – 4061Phosphoserine1 Publication
Modified residuei410 – 4101Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6P7W5.
PRIDEiQ6P7W5.

PTM databases

PhosphoSiteiQ6P7W5.

Expressioni

Gene expression databases

BgeeiQ6P7W5.
GenevestigatoriQ6P7W5.

Interactioni

Subunit structurei

tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54. tRNA splicing endonuclease complex also contains proteins of the pre-mRNA 3'-end processing machinery such as CLP1, CPSF1, CPSF4 and CSTF2 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ6P7W5.
SMRiQ6P7W5. Positions 292-415.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family.Curated

Phylogenomic databases

eggNOGiCOG1676.
GeneTreeiENSGT00390000013266.
HOGENOMiHOG000154285.
HOVERGENiHBG056610.
InParanoidiQ6P7W5.
KOiK15322.
OMAiKLVCRRN.
OrthoDBiEOG76X623.
PhylomeDBiQ6P7W5.
TreeFamiTF314679.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR016589. tRNA_splic_SEN2.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view]
PIRSFiPIRSF011789. tRNA_splic_SEN2. 1 hit.
SUPFAMiSSF53032. SSF53032. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6P7W5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAEAVFRAPK RKRRVYESYE SPLPIPFGQD QGPRKEFRIF QAEMISNNVV
60 70 80 90 100
VRGTEDMEQL YGKGYFGKGI LSRSRPNFTI ANPTLAARWK GVQTDMPIIT
110 120 130 140 150
SEKYQHRVEW ARDFLRRQGH DESTVQKILT DYTEPLELPC REEKEETPQH
160 170 180 190 200
EPLSSKADSS LEGRVEKDEL PVTPGGAGQS DDLPGLGTHS DCLQEGPGHA
210 220 230 240 250
TLAAASPSSH NGHVAEDPEV LPQETLVPQG GLWPEASSQA AGEKRAAHEY
260 270 280 290 300
VLIEEELCGA QEEEAAAASD EKLLKRKKLV CRRNPYRIFE YLQLSLEEAF
310 320 330 340 350
FLAYALGCLS IYYEKEPLTI VKLWQAFTAV QPTFRTTYMA YHYFRSKGWV
360 370 380 390 400
PKVGLKYGTD LLLYRKGPPF YHASYSVIIE LLDDNYEGSL RRPFSWKSLA
410 420 430 440 450
ALSRVSGNVS KELMLCYLIK PSTMTAEDME TPECMKRIQV QEVILSRWVS
460
SRERSDQDEL
Length:460
Mass (Da):52,214
Last modified:July 5, 2004 - v1
Checksum:i1FC32E811F488184
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK134697 mRNA. Translation: BAE22246.1.
BC061473 mRNA. Translation: AAH61473.1.
CCDSiCCDS20440.1.
RefSeqiNP_950198.1. NM_199033.1.
UniGeneiMm.291208.

Genome annotation databases

EnsembliENSMUST00000040234; ENSMUSP00000038211; ENSMUSG00000042389.
GeneIDi381802.
KEGGimmu:381802.
UCSCiuc009diu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK134697 mRNA. Translation: BAE22246.1 .
BC061473 mRNA. Translation: AAH61473.1 .
CCDSi CCDS20440.1.
RefSeqi NP_950198.1. NM_199033.1.
UniGenei Mm.291208.

3D structure databases

ProteinModelPortali Q6P7W5.
SMRi Q6P7W5. Positions 292-415.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q6P7W5.

Proteomic databases

MaxQBi Q6P7W5.
PRIDEi Q6P7W5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000040234 ; ENSMUSP00000038211 ; ENSMUSG00000042389 .
GeneIDi 381802.
KEGGi mmu:381802.
UCSCi uc009diu.1. mouse.

Organism-specific databases

CTDi 80746.
MGIi MGI:2141599. Tsen2.

Phylogenomic databases

eggNOGi COG1676.
GeneTreei ENSGT00390000013266.
HOGENOMi HOG000154285.
HOVERGENi HBG056610.
InParanoidi Q6P7W5.
KOi K15322.
OMAi KLVCRRN.
OrthoDBi EOG76X623.
PhylomeDBi Q6P7W5.
TreeFami TF314679.

Miscellaneous databases

NextBioi 402575.
PROi Q6P7W5.
SOURCEi Search...

Gene expression databases

Bgeei Q6P7W5.
Genevestigatori Q6P7W5.

Family and domain databases

Gene3Di 3.40.1350.10. 1 hit.
InterProi IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR016589. tRNA_splic_SEN2.
[Graphical view ]
Pfami PF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF011789. tRNA_splic_SEN2. 1 hit.
SUPFAMi SSF53032. SSF53032. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Medulla oblongata.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-403; SER-406 AND SER-410, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.

Entry informationi

Entry nameiSEN2_MOUSE
AccessioniPrimary (citable) accession number: Q6P7W5
Secondary accession number(s): Q3UYG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3