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Protein

tRNA-splicing endonuclease subunit Sen2

Gene

Tsen2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei364By similarity1
Active sitei372By similarity1
Active sitei411By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processmRNA processing, tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease subunit Sen2 (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease Sen2
Gene namesi
Name:Tsen2
Synonyms:Sen2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2141599 Tsen2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094531 – 460tRNA-splicing endonuclease subunit Sen2Add BLAST460

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei403PhosphoserineCombined sources1
Modified residuei406PhosphoserineCombined sources1
Modified residuei410PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6P7W5
MaxQBiQ6P7W5
PaxDbiQ6P7W5
PeptideAtlasiQ6P7W5
PRIDEiQ6P7W5

PTM databases

iPTMnetiQ6P7W5
PhosphoSitePlusiQ6P7W5

Expressioni

Gene expression databases

BgeeiENSMUSG00000042389
ExpressionAtlasiQ6P7W5 baseline and differential
GenevisibleiQ6P7W5 MM

Interactioni

Subunit structurei

tRNA splicing endonuclease is a heterotetramer composed of TSEN2, TSEN15, TSEN34/LENG5 and TSEN54. tRNA splicing endonuclease complex also contains proteins of the pre-mRNA 3'-end processing machinery such as CLP1, CPSF1, CPSF4 and CSTF2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038211

Structurei

3D structure databases

ProteinModelPortaliQ6P7W5
SMRiQ6P7W5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family.Curated

Phylogenomic databases

eggNOGiKOG4685 Eukaryota
COG1676 LUCA
GeneTreeiENSGT00390000013266
HOGENOMiHOG000154285
HOVERGENiHBG056610
InParanoidiQ6P7W5
KOiK15322
OMAiTFEETFF
OrthoDBiEOG091G0FMD
PhylomeDBiQ6P7W5
TreeFamiTF314679

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006678 tRNA_intron_Endonuc_N
IPR006676 tRNA_splic
IPR016589 tRNA_splic_SEN2
PANTHERiPTHR21227 PTHR21227, 1 hit
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 1 hit
PF02778 tRNA_int_endo_N, 1 hit
PIRSFiPIRSF011789 tRNA_splic_SEN2, 1 hit
SUPFAMiSSF53032 SSF53032, 1 hit

Sequencei

Sequence statusi: Complete.

Q6P7W5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEAVFRAPK RKRRVYESYE SPLPIPFGQD QGPRKEFRIF QAEMISNNVV
60 70 80 90 100
VRGTEDMEQL YGKGYFGKGI LSRSRPNFTI ANPTLAARWK GVQTDMPIIT
110 120 130 140 150
SEKYQHRVEW ARDFLRRQGH DESTVQKILT DYTEPLELPC REEKEETPQH
160 170 180 190 200
EPLSSKADSS LEGRVEKDEL PVTPGGAGQS DDLPGLGTHS DCLQEGPGHA
210 220 230 240 250
TLAAASPSSH NGHVAEDPEV LPQETLVPQG GLWPEASSQA AGEKRAAHEY
260 270 280 290 300
VLIEEELCGA QEEEAAAASD EKLLKRKKLV CRRNPYRIFE YLQLSLEEAF
310 320 330 340 350
FLAYALGCLS IYYEKEPLTI VKLWQAFTAV QPTFRTTYMA YHYFRSKGWV
360 370 380 390 400
PKVGLKYGTD LLLYRKGPPF YHASYSVIIE LLDDNYEGSL RRPFSWKSLA
410 420 430 440 450
ALSRVSGNVS KELMLCYLIK PSTMTAEDME TPECMKRIQV QEVILSRWVS
460
SRERSDQDEL
Length:460
Mass (Da):52,214
Last modified:July 5, 2004 - v1
Checksum:i1FC32E811F488184
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK134697 mRNA Translation: BAE22246.1
BC061473 mRNA Translation: AAH61473.1
CCDSiCCDS20440.1
RefSeqiNP_950198.1, NM_199033.1
UniGeneiMm.291208

Genome annotation databases

EnsembliENSMUST00000040234; ENSMUSP00000038211; ENSMUSG00000042389
GeneIDi381802
KEGGimmu:381802
UCSCiuc009diu.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSEN2_MOUSE
AccessioniPrimary (citable) accession number: Q6P7W5
Secondary accession number(s): Q3UYG6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 5, 2004
Last modified: March 28, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health