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Protein

SH3KBP1-binding protein 1

Gene

Shkbp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits CBL-SH3KBP1 complex mediated down-regulation of EGFR signaling by sequestration of SH3KBP1. Binds to SH3KBP1 and prevents its interaction with CBL and inhibits translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation (PubMed:21830225).1 Publication

GO - Biological processi

  • positive regulation of epidermal growth factor receptor signaling pathway Source: UniProtKB
  • protein homooligomerization Source: InterPro
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
SH3KBP1-binding protein 1
Alternative name(s):
SETA-binding protein 1
Gene namesi
Name:Shkbp1
Synonyms:Sb1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2385803. Shkbp1.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi623 – 6231R → A: No loss of interaction with SH3KBP1, loss of translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation, strong delay in the degradation of EGFR upon EGF activation and enhancement of EGFR signaling induced serum response element (SRE) transcriptional reporter activity. Loss of interaction with SH3KBP1, no loss of translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation, no effect on the degradation of EGFR upon EGF activation and no effect on EGFR signaling induced serum response element (SRE) transcriptional reporter activity; when associated with A-683. 1 Publication
Mutagenesisi683 – 6831R → A: No loss of interaction with SH3KBP1, loss of translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation, slight delay in the degradation of EGFR upon EGF activation and enhancement of EGFR signaling induced serum response element (SRE) transcriptional reporter activity. Loss of interaction with SH3KBP1, no loss of translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation, no effect on the degradation of EGFR upon EGF activation and no effect on EGFR signaling induced serum response element (SRE) transcriptional reporter activity; when associated with A-623. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 704703SH3KBP1-binding protein 1PRO_0000307933Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei163 – 1631PhosphothreonineBy similarity
Modified residuei644 – 6441PhosphoserineBy similarity
Modified residuei646 – 6461PhosphoserineBy similarity
Modified residuei693 – 6931PhosphothreonineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ6P7W2.
PaxDbiQ6P7W2.
PeptideAtlasiQ6P7W2.
PRIDEiQ6P7W2.

PTM databases

iPTMnetiQ6P7W2.
PhosphoSiteiQ6P7W2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000089832.
CleanExiMM_SHKBP1.
GenevisibleiQ6P7W2. MM.

Interactioni

Subunit structurei

Interacts (via PXXXPR motifs) with SH3KBP1 (via SH3 domains).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Pdzk1Q9JJ407EBI-7713890,EBI-7713572From a different organism.

Protein-protein interaction databases

BioGridi228669. 1 interaction.
IntActiQ6P7W2. 1 interaction.
MINTiMINT-1893516.
STRINGi10090.ENSMUSP00000003857.

Structurei

3D structure databases

ProteinModelPortaliQ6P7W2.
SMRiQ6P7W2. Positions 18-120, 257-316, 429-457.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 8870BTBPROSITE-ProRule annotationAdd
BLAST
Repeati233 – 28048WD 1Add
BLAST
Repeati283 – 32240WD 2Add
BLAST
Repeati324 – 35936WD 3Add
BLAST
Repeati428 – 46639WD 4Add
BLAST
Repeati548 – 58639WD 5Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi618 – 6236PXXXPR1 Publication
Motifi678 – 6836PXXXPR1 Publication

Sequence similaritiesi

Belongs to the KCTD3 family.Curated
Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 5 WD repeats.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2714. Eukaryota.
ENOG410XSWI. LUCA.
GeneTreeiENSGT00530000062980.
HOGENOMiHOG000006656.
HOVERGENiHBG061001.
InParanoidiQ6P7W2.
OMAiIQKVVPN.
OrthoDBiEOG091G0391.
PhylomeDBiQ6P7W2.
TreeFamiTF313754.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF02214. BTB_2. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
SSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6P7W2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVATTAVEG VPSRGAPGEV IHLNVGGKRF STSRQTLTWI PDSFFSSLLS
60 70 80 90 100
GRISTLKDET GAIFIDRDPT VFAPILNFLR TKELDPRGVH GSSLLHEAQF
110 120 130 140 150
YGLTPLVRRL QVREELDRSS CGNVLFNGYL PPPVFPVKRR NRHSLVGPQQ
160 170 180 190 200
IGGRPAPVRR SNTMPPNLGN AGLLGRMLDE RAPPSPSGQP EEPGMVRLVC
210 220 230 240 250
GHHNWIAVAY THFLVCYRLK EASGWQLAFS SPRLDWPIER LALAARVLGG
260 270 280 290 300
APGEHDKMVA AATGSEILLW ALQAQGGGSE IGVFHLGVPV EALFFVGNQL
310 320 330 340 350
IATSHTGRIG VWNAVTKHWQ VQEVQPITSY DAAGSFLLLG CSNGSIYYVD
360 370 380 390 400
VQKFPLRMKD NDLLVSELYR DPAEDGVTAL SVYLTPKTSD SGNWIEIAYG
410 420 430 440 450
TSSGVVRVIV QHPETVGSGP QLFQTFSVHR SPVTKIMLSE KHLISVCADN
460 470 480 490 500
NHVRTWSVTR FRGMISTQPG STPLASFKIL ALESVDGLGG CSAGNDIGPY
510 520 530 540 550
GERDDQQVFI QKVVPNASQL FVRLSSTGQR VCSVRSVDGS PTTAFTVLEC
560 570 580 590 600
EGSRRLGSRP RRYLLTGQAN GSLAMWDLTT AMDGLGQTPA GGLTEEELMD
610 620 630 640 650
QLEQCELSPL ASSRGSFPSP SPRTSLTSLH SASSNTSLYG PRGSPSPPQA
660 670 680 690 700
EARRRGAGSF VDRCQELARG APELRWPPTP APRPSTSLGN PLTALKKTLN

ETSF
Length:704
Mass (Da):76,103
Last modified:July 5, 2004 - v1
Checksum:iE6AB971825347A8C
GO
Isoform 2 (identifier: Q6P7W2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-163: Missing.
     590-590: Missing.

Note: No experimental confirmation available.
Show »
Length:540
Mass (Da):58,106
Checksum:i7469D2ECDC0E3AD4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41A → P in AAG13656 (PubMed:11152963).Curated
Sequence conflicti12 – 121P → R in AAG13656 (PubMed:11152963).Curated
Sequence conflicti430 – 4301R → S in BAE35933 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 163163Missing in isoform 2. 1 PublicationVSP_028875Add
BLAST
Alternative sequencei590 – 5901Missing in isoform 2. 1 PublicationVSP_028876

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF246218 mRNA. Translation: AAG13656.1.
AK160641 mRNA. Translation: BAE35933.1.
BC061477 mRNA. Translation: AAH61477.1.
CCDSiCCDS21018.1. [Q6P7W2-1]
RefSeqiNP_619617.2. NM_138676.2. [Q6P7W2-1]
UniGeneiMm.21297.
Mm.470999.

Genome annotation databases

EnsembliENSMUST00000003857; ENSMUSP00000003857; ENSMUSG00000089832. [Q6P7W2-1]
GeneIDi192192.
KEGGimmu:192192.
UCSCiuc009fvv.1. mouse. [Q6P7W2-1]
uc009fvy.1. mouse. [Q6P7W2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF246218 mRNA. Translation: AAG13656.1.
AK160641 mRNA. Translation: BAE35933.1.
BC061477 mRNA. Translation: AAH61477.1.
CCDSiCCDS21018.1. [Q6P7W2-1]
RefSeqiNP_619617.2. NM_138676.2. [Q6P7W2-1]
UniGeneiMm.21297.
Mm.470999.

3D structure databases

ProteinModelPortaliQ6P7W2.
SMRiQ6P7W2. Positions 18-120, 257-316, 429-457.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228669. 1 interaction.
IntActiQ6P7W2. 1 interaction.
MINTiMINT-1893516.
STRINGi10090.ENSMUSP00000003857.

PTM databases

iPTMnetiQ6P7W2.
PhosphoSiteiQ6P7W2.

Proteomic databases

MaxQBiQ6P7W2.
PaxDbiQ6P7W2.
PeptideAtlasiQ6P7W2.
PRIDEiQ6P7W2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003857; ENSMUSP00000003857; ENSMUSG00000089832. [Q6P7W2-1]
GeneIDi192192.
KEGGimmu:192192.
UCSCiuc009fvv.1. mouse. [Q6P7W2-1]
uc009fvy.1. mouse. [Q6P7W2-2]

Organism-specific databases

CTDi92799.
MGIiMGI:2385803. Shkbp1.

Phylogenomic databases

eggNOGiKOG2714. Eukaryota.
ENOG410XSWI. LUCA.
GeneTreeiENSGT00530000062980.
HOGENOMiHOG000006656.
HOVERGENiHBG061001.
InParanoidiQ6P7W2.
OMAiIQKVVPN.
OrthoDBiEOG091G0391.
PhylomeDBiQ6P7W2.
TreeFamiTF313754.

Miscellaneous databases

ChiTaRSiShkbp1. mouse.
PROiQ6P7W2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000089832.
CleanExiMM_SHKBP1.
GenevisibleiQ6P7W2. MM.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF02214. BTB_2. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
SSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSHKB1_MOUSE
AccessioniPrimary (citable) accession number: Q6P7W2
Secondary accession number(s): Q3TUN8, Q9ES31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.