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Protein

Sentrin-specific protease 6

Gene

Senp6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. Processes preferentially poly-SUMO2 and poly-SUMO3 chains, but does not efficiently process SUMO1, SUMO2 and SUMO3 precursors. Deconjugates SUMO1 from RXRA, leading to transcriptional activation. Involved in chromosome alignment and spindle assembly, by regulating the kinetochore CENPH-CENPI-CENPK complex. Desumoylates PML and CENPI, protecting them from degradation by the ubiquitin ligase RNF4, which targets polysumoylated proteins for proteasomal degradation. Desumoylates also RPA1, thus preventing recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination.

Catalytic activityi

Hydrolysis of the alpha-linked peptide bond in the sequence Gly-Gly-|-Ala-Thr-Tyr at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptide, Smt3, leading to the mature form of the protein. A second reaction involves the cleavage of an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein.

Pathwayi: protein sumoylation

This protein is involved in the pathway protein sumoylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei785By similarity1
Active sitei936By similarity1
Active sitei1049By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00886.

Protein family/group databases

MEROPSiC48.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Sentrin-specific protease 6 (EC:3.4.22.68)
Alternative name(s):
SUMO-1-specific protease 1
Sentrin/SUMO-specific protease SENP6
Gene namesi
Name:Senp6
Synonyms:Kiaa0797, Susp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1922075. Senp6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002676081 – 1132Sentrin-specific protease 6Add BLAST1132

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei41PhosphoserineBy similarity1
Modified residuei355PhosphoserineCombined sources1
Modified residuei356PhosphoserineCombined sources1
Modified residuei371PhosphoserineBy similarity1
Modified residuei373PhosphoserineBy similarity1
Modified residuei436PhosphothreonineBy similarity1
Modified residuei938PhosphoserineBy similarity1
Modified residuei1131PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6P7W0.
PeptideAtlasiQ6P7W0.
PRIDEiQ6P7W0.

PTM databases

iPTMnetiQ6P7W0.
PhosphoSitePlusiQ6P7W0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000034252.
CleanExiMM_SENP6.
ExpressionAtlasiQ6P7W0. baseline and differential.
GenevisibleiQ6P7W0. MM.

Interactioni

Subunit structurei

Interacts with RXRA. Forms a complex with KAT5-TIP60 and UBE2I in response to UV irradiation. Interacts with RPA1 to maintain it in hyposumoylated state during S phase preventing DNA repair initiation (By similarity).By similarity

Protein-protein interaction databases

BioGridi229616. 2 interactors.
IntActiQ6P7W0. 2 interactors.
MINTiMINT-8368418.
STRINGi10090.ENSMUSP00000047220.

Structurei

3D structure databases

ProteinModelPortaliQ6P7W0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni686 – 1132ProteaseAdd BLAST447

Sequence similaritiesi

Belongs to the peptidase C48 family.Curated

Phylogenomic databases

eggNOGiKOG0779. Eukaryota.
COG5160. LUCA.
GeneTreeiENSGT00530000063531.
HOVERGENiHBG059746.
InParanoidiQ6P7W0.
KOiK08595.
TreeFamiTF350136.

Family and domain databases

InterProiIPR003653. Peptidase_C48_C.
[Graphical view]
PfamiPF02902. Peptidase_C48. 2 hits.
[Graphical view]
PROSITEiPS50600. ULP_PROTEASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6P7W0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAGKSGGSA GALFLKALDR SESKRDGGFK NNWSFDHEEE SEGDADKDGA
60 70 80 90 100
NLLSVEDEDS EISKGKKLNR RSEIVATSSG DFILKTYVRR SKTDGFKTLK
110 120 130 140 150
GNPIGLNMLS NNKKLSESTA GTALCSGTVV HGRRFHHAHS QTPGIRTAAQ
160 170 180 190 200
RKEYPPYVHK AENSPVMLSH GQGGDHIMKK TEESESYVES EIKRKVQQKR
210 220 230 240 250
HCSTYQLSPL SPASKKCLTH LEVSEQREYC PKCGKEKENQ TKCQSCGIVF
260 270 280 290 300
HNDLQRNCRQ AVTLNEPTGP LLRTSIHQNS GGQKSQNTGL TAKKFYGNSV
310 320 330 340 350
DKIPIDILVT CDDSRHNYIQ TNGKVILPGG KIPKLTNPKE RKISVSDLND
360 370 380 390 400
PIILSSDDDD DDDDRTKRRE STSPKPADSA CSSPVPSTGK VEAALNADAC
410 420 430 440 450
RAEQEPRSSP AEPELNTIVI PRKARMKDQL GNSISTPLKR RKVNSHAAFI
460 470 480 490 500
HPMSLSCQNF ESVILNCRSI RVGTLFRLLV EPVIFSLESI TIHLDGPESD
510 520 530 540 550
PVDIILNTSD LTKCEWCNVR KLPVVFLQAI PAVYQKLSMQ LQMSKEDKVW
560 570 580 590 600
NDCKGINRIT SLEEQYIILI FQTGLDHQAE VVFESIITDI GIRNNVPNFF
610 620 630 640 650
AKILFDEANS RLVACTRSYE ESIKGNCAQK ENKVKTVSFE SKIQLRSKQE
660 670 680 690 700
LQFFDDDEEA GESHTIFIGP VEKLIVYPPP PAKGGISVTN EDLHCLSEGE
710 720 730 740 750
FLNDVIIDFY LKYLVLEKLK KEDADRIHIF SSFFYKRLNQ RERRNPETTN
760 770 780 790 800
LSIQQKRHGR VKTWTRHVDI FEKDFIFVPL NEAAHWFLAV VCFPGLEKPK
810 820 830 840 850
YEPNPHYHEN AVMQKTPSAE DSCVSSASEM GACSQNSAAK PVIKKMLNRK
860 870 880 890 900
HCLAVTDSSA AQEESEPCYR RNAYSVKCSM KKKNHAINEN EEPSNGESTC
910 920 930 940 950
QDICDRTQSE NGLRDECFSS VHHPDALSKI RLNYGDQSAD GGKLLEDELI
960 970 980 990 1000
DFSEDQDDPD DSSDDGLLAD ENYSSEIGQW HLKPTVCKQP CILLMDSLRG
1010 1020 1030 1040 1050
PSRSNVVKIL REYLEVEWEV KKGSKRSFSK DVMKGSNPKV PQQNNFSDCG
1060 1070 1080 1090 1100
VYVLQYVESF FENPVLNFEL PMNLMNWFPP PRMKTKREEI RNIILKLQES
1110 1120 1130
QSKDKKLLKD SLAETSLGDG AEQYASASGG SE
Length:1,132
Mass (Da):127,013
Last modified:July 27, 2011 - v3
Checksum:i7D9BE2AE1202ECFF
GO
Isoform 2 (identifier: Q6P7W0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-166: Missing.

Note: No experimental confirmation available.
Show »
Length:966
Mass (Da):109,037
Checksum:iABA14523EB9875B0
GO

Sequence cautioni

The sequence AAH61480 differs from that shown. Reason: Frameshift at position 815.Curated
The sequence BAC35583 differs from that shown. Probable cloning artifact.Curated
The sequence BAC98029 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti121G → A in AAH61480 (PubMed:15489334).Curated1
Sequence conflicti375K → Q in BAC98029 (PubMed:14621295).Curated1
Sequence conflicti375K → Q in AAH61480 (PubMed:15489334).Curated1
Sequence conflicti747E → D in BAC98029 (PubMed:14621295).Curated1
Sequence conflicti747E → D in AAH61480 (PubMed:15489334).Curated1
Sequence conflicti1075M → V in BAC98029 (PubMed:14621295).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0219431 – 166Missing in isoform 2. 1 PublicationAdd BLAST166

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129219 mRNA. Translation: BAC98029.1. Different initiation.
AC142111 Genomic DNA. No translation available.
BC061480 mRNA. Translation: AAH61480.1. Frameshift.
AK053904 mRNA. Translation: BAC35583.1. Sequence problems.
AK083377 mRNA. Translation: BAC38892.2.
CCDSiCCDS40705.1. [Q6P7W0-1]
RefSeqiNP_001298039.1. NM_001311110.1.
NP_666115.2. NM_146003.2. [Q6P7W0-1]
UniGeneiMm.28232.

Genome annotation databases

EnsembliENSMUST00000037484; ENSMUSP00000047220; ENSMUSG00000034252. [Q6P7W0-1]
ENSMUST00000164859; ENSMUSP00000128918; ENSMUSG00000034252. [Q6P7W0-2]
GeneIDi215351.
KEGGimmu:215351.
UCSCiuc009qvb.1. mouse. [Q6P7W0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129219 mRNA. Translation: BAC98029.1. Different initiation.
AC142111 Genomic DNA. No translation available.
BC061480 mRNA. Translation: AAH61480.1. Frameshift.
AK053904 mRNA. Translation: BAC35583.1. Sequence problems.
AK083377 mRNA. Translation: BAC38892.2.
CCDSiCCDS40705.1. [Q6P7W0-1]
RefSeqiNP_001298039.1. NM_001311110.1.
NP_666115.2. NM_146003.2. [Q6P7W0-1]
UniGeneiMm.28232.

3D structure databases

ProteinModelPortaliQ6P7W0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229616. 2 interactors.
IntActiQ6P7W0. 2 interactors.
MINTiMINT-8368418.
STRINGi10090.ENSMUSP00000047220.

Protein family/group databases

MEROPSiC48.004.

PTM databases

iPTMnetiQ6P7W0.
PhosphoSitePlusiQ6P7W0.

Proteomic databases

PaxDbiQ6P7W0.
PeptideAtlasiQ6P7W0.
PRIDEiQ6P7W0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037484; ENSMUSP00000047220; ENSMUSG00000034252. [Q6P7W0-1]
ENSMUST00000164859; ENSMUSP00000128918; ENSMUSG00000034252. [Q6P7W0-2]
GeneIDi215351.
KEGGimmu:215351.
UCSCiuc009qvb.1. mouse. [Q6P7W0-1]

Organism-specific databases

CTDi26054.
MGIiMGI:1922075. Senp6.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0779. Eukaryota.
COG5160. LUCA.
GeneTreeiENSGT00530000063531.
HOVERGENiHBG059746.
InParanoidiQ6P7W0.
KOiK08595.
TreeFamiTF350136.

Enzyme and pathway databases

UniPathwayiUPA00886.

Miscellaneous databases

ChiTaRSiSenp6. mouse.
PROiQ6P7W0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034252.
CleanExiMM_SENP6.
ExpressionAtlasiQ6P7W0. baseline and differential.
GenevisibleiQ6P7W0. MM.

Family and domain databases

InterProiIPR003653. Peptidase_C48_C.
[Graphical view]
PfamiPF02902. Peptidase_C48. 2 hits.
[Graphical view]
PROSITEiPS50600. ULP_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSENP6_MOUSE
AccessioniPrimary (citable) accession number: Q6P7W0
Secondary accession number(s): E9QN09
, Q6ZQ43, Q8BK94, Q8BUL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.