Reviewed, UniProtKB/Swiss-Prot Q6P7S1 (ASAH1_RAT)

Names and origin

Protein names

Recommended name:
    Acid ceramidase
      Short name=AC
    EC=3.5.1.23
Alternative name(s):
    Acylsphingosine deacylase
    N-acylsphingosine amidohydrolase

Gene names

Name:

Asah1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid By similarity.
Catalytic activity	N-acylsphingosine + H2O = a carboxylate + sphingosine.
Subunit structure	Heterodimer of one alpha and one beta subunit By similarity.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the acid ceramidase family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	lipid metabolic process Inferred from electronic annotation. Source: InterPro lung development Inferred from direct assay. Source: RGD response to organic substance Inferred from direct assay. Source: RGD
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ceramidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 394	374	Acid ceramidase		PRO_0000378102
Amino acid modifications
	Glycosylation	194	1	N-linked (GlcNAc...) Potential
	Glycosylation	258	1	N-linked (GlcNAc...) Potential
	Glycosylation	285	1	N-linked (GlcNAc...) Potential
	Glycosylation	341	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	375	1	V → G in AAG43956. Ref.1
	Sequence conflict	379	1	Q → P in AAG43956. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q6P7S1-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 27608539E1E9ED20	FASTA	394	44,443
	10 20 30 40 50 60 MLGRSLLTWV LAAAVTCAQA QQVPPWTEDC RKSTYPPSGP TYRGPVPWYT INLDLPPYKR 70 80 90 100 110 120 WHELLAHKAP VLRTLVNSIS NLVNAFVPSG KIMQMVDEKL PGLIGSIPGP FGEEMRGIAD 130 140 150 160 170 180 VTGIPLGEII SFNIFYELFT MCTSIITEDG KGHLLHGRNM DFGIFLGWNI NNNTWVVTEE 190 200 210 220 230 240 LKPLTVNLDF QRNNKTVFKA TSFAGYVGML TGFKPGLLSL TLNERFSLNG GYLGILEWMF 250 260 270 280 290 300 GKKNAQWVGF ITRSVLENST SYEEAKNILT KTKITAPAYF ILGGNQSGEG CVITRERKES 310 320 330 340 350 360 LDVYELDPKH GRWYVVQTNY DRWKNTLFLD DRRTPAKKCL NHTTQKNLSF ATIYDVLSTK 370 380 390 PVLNKLTVFT TLIDVTKDQF ESHLRDCPDP CIGW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete coding sequence of rat ceramidase." Frenck J., Sol-Church K., McKay C., Mason R. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF214647 mRNA. Translation: AAG43956.1. BC061540 mRNA. Translation: AAH61540.1.
IPI	IPI00421601.
RefSeq	NP_445859.2.
UniGene	Rn.127798
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6P7S1.
Proteomic databases
PRIDE	Q6P7S1.
Genome annotation databases
Ensembl	ENSRNOT00000013463; ENSRNOP00000013463; ENSRNOG00000010034; Rattus norvegicus. [Genome view]
GeneID	84431.
KEGG	rno:84431.
NMPDR	fig|10116.3.peg.12488.
UCSC	NM_053407. rat.
Organism-specific databases
CTD	84431.
RGD	621568. Asah1.
Phylogenomic databases
HOVERGEN	Q6P7S1.
OMA	NYDRWKN.
Gene expression databases
ArrayExpress	Q6P7S1.
Genevestigator	Q6P7S1.
Family and domain databases
InterPro	IPR016699. Acid_ceramidase-like. IPR003199. Chologlycine_hydro. [Graphical view]
Pfam	PF02275. CBAH. 1 hit. [Graphical view]
PIRSF	PIRSF017632. Acid_ceramidase-like. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	616940.

Entry information

Entry name

ASAH1_RAT

Accession

Primary (citable) accession number: Q6P7S1
Secondary accession number(s): Q9EQJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)