Reviewed,
UniProtKB/Swiss-Prot Q6P7R8 (DHB12_RAT)
Last modified
November 25, 2008.
Version 41.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Estradiol 17-beta-dehydrogenase 12 EC=1.1.1.62 Alternative name(s): 17-beta-hydroxysteroid dehydrogenase 12 Short name=17-beta-HSD 12 3-ketoacyl-CoA reductase Short name=KAR EC=1.3.1.- | ||
| Gene names |
| ||
| Organism | Rattus norvegicus (Rat) | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 312 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation. Its strong expression in ovary and mammary gland suggest that it may constitute the major enzyme responsible for the conversion of E1 to E2 in females. Also has 3-ketoacyl-CoA reductase activity, reducing both long chain 3-ketoacyl-CoAs and long chain fatty acyl-CoAs, suggesting a role in long fatty acid elongation By similarity. |
| Catalytic activity | Estradiol-17-beta + NAD(P)(+) = estrone + NAD(P)H. |
| Pathway | |
| Subcellular location | Endoplasmic reticulum membrane; Multi-pass membrane proteinBy similarity. |
| Domain | The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins By similarity. |
| Sequence similarities | Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily. |
| Sequence caution | The sequence AAD00504.1 differs from that shown. Reason: Frameshift at position 272. |
Ontologies
Keywords | |
|---|---|
| Biological process | Lipid synthesis Steroid biosynthesis |
| Cellular component | Endoplasmic reticulum Membrane |
| Domain | Transmembrane |
| Ligand | NADP |
| Molecular function | Oxidoreductase |
Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | binding Inferred from electronic annotation. Source: InterPro estradiol 17-beta-dehydrogenase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 312 | 312 | Estradiol 17-beta-dehydrogenase 12 | PRO_0000248370 | |||||
Regions | |||||||||
| Transmembrane | 4 – 24 | 21 | Potential | ||||||
| Transmembrane | 182 – 202 | 21 | Potential | ||||||
| Transmembrane | 271 – 291 | 21 | Potential | ||||||
| Nucleotide binding | 50 – 79 | 30 | NADP By similarity | ||||||
| Motif | 308 – 312 | 5 | Di-lysine motif By similarity | ||||||
Sites | |||||||||
| Active site | 202 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 189 | 1 | Substrate By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 236 | 1 | A → S in AAD00504. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | Trzyna W.C., Gabbeta V., McHugh K.M. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary anterior lobe. |
Cross-references
Sequence databases | |
|---|---|
| U81186 mRNA. Translation: AAD00504.1. Frameshift. BC061543 mRNA. Translation: AAH61543.1. | |
| RefSeq | NP_114455.1. |
| UniGene | Rn.203283 |
3D structure databases | |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | Q6P7R8. |
Genome annotation databases | |
| Ensembl | ENSRNOG00000009630. Rattus norvegicus. [Contig view] |
| GeneID | 84013. |
| KEGG | rno:84013. |
| NMPDR | fig|10116.3.peg.19242. |
Organism-specific databases | |
| RGD | 708367. Hsd17b12. |
Phylogenomic databases | |
| HOVERGEN | Q6P7R8. |
Gene expression databases | |
| ArrayExpress | Q6P7R8. |
| GermOnline | ENSRNOG00000009630. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR002198. DHase_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DHase. IPR016040. NAD(P)-bd. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| PANTHER | PTHR19410. ADH_short_C2. 1 hit. |
| Pfam | PF00106. adh_short. 1 hit. [Graphical view] |
| PRINTS | PR00081. GDHRDH. PR00080. SDRFAMILY. |
| PROSITE | PS00061. ADH_SHORT. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 616547. |
Entry information
| Entry name | DHB12_RAT | ||||||||
| Accession | Primary (citable) accession number: Q6P7R8 Secondary accession number(s): Q9Z1B9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


