Q6P7Q4 (LGUL_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lactoylglutathione lyase
EC=4.4.1.5

Alternative name(s):
Aldoketomutase
Glyoxalase I
Short name=Glx I
Ketone-aldehyde mutase
Methylglyoxalase
S-D-lactoylglutathione methylglyoxal lyase

Gene names

Name:

Glo1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B By similarity.
Catalytic activity	(R)-S-lactoylglutathione = glutathione + methylglyoxal.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
Subunit structure	Homodimer By similarity.
Post-translational modification	Glutathionylation at Cys-139 inhibits enzyme activity By similarity. Phosphorylated at Thr-107 in the presence of CaMK2. However, this is a consensus site for phosphorylation by CK2 so phosphorylation may be mediated by CK2 rather than CaMK2. Phosphorylation is induced by TNF and suppresses the TNF-induced transcriptional activity of NF-kappa-B By similarity. Exists in a nitric oxide (NO)-modified form. The exact nature of the modification is unknown, but it suppresses the TNF-induced transcriptional activity of NF-kappa-B By similarity.
Sequence similarities	Belongs to the glyoxalase I family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Lyase
PTM	Acetylation Disulfide bond Glutathionylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from direct assay PubMed 8719777PubMed 8903102. Source: RGD glutathione metabolic process Inferred from direct assay PubMed 8719777PubMed 8903102. Source: RGD methylglyoxal metabolic process Inferred from direct assay PubMed 10712823PubMed 8719777PubMed 8903102. Source: RGD negative regulation of apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara
Molecular_function	lactoylglutathione lyase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 184	183	Lactoylglutathione lyase		PRO_0000168079
Sites
	Metal binding	34	1	Zinc By similarity
	Metal binding	100	1	Zinc By similarity
	Metal binding	127	1	Zinc By similarity
	Metal binding	173	1	Zinc By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	107	1	Phosphothreonine By similarity
	Modified residue	139	1	S-glutathionyl cysteine By similarity
	Modified residue	148	1	N6-acetyllysine By similarity
	Disulfide bond	19 ↔ 20		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6P7Q4 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 1834FA5E9871A1C7	FASTA	184	20,820
	10 20 30 40 50 60 MAEPQPASSG LTDEAALSCC SDPDPSTKDF LLQQTMLRIK DPKKSLDFYT RVLGLTLLQK 70 80 90 100 110 120 LDFPSMKFSL YFLAYEDKND IPKDKTERTA WAFSRKATLE LTHNWGTEDD ETQSYHNGNS 130 140 150 160 170 180 DPRGFGHIGI AVPDVYEACK RFEELGVKFV KKPDDGKMKG LAFVQDPDGY WIEILNPNKM ATII

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
[2]	Lubec G., Afjehi-Sadat L., Chen W.-Q. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 29-38; 44-83; 89-95 AND 124-148, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus and Spinal cord.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC061570 mRNA. Translation: AAH61570.1.
IPI	IPI00188304.
RefSeq	NP_997477.1. NM_207594.2.
UniGene	Rn.108014.
3D structure databases
ProteinModelPortal	Q6P7Q4.
SMR	Q6P7Q4. Positions 4-183.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000000650.
Proteomic databases
PaxDb	Q6P7Q4.
PRIDE	Q6P7Q4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000000650; ENSRNOP00000000650; ENSRNOG00000000541.
GeneID	294320.
KEGG	rno:294320.
UCSC	RGD:2702. rat.
Organism-specific databases
CTD	2739.
RGD	2702. Glo1.
Phylogenomic databases
eggNOG	COG0346.
GeneTree	ENSGT00390000009312.
HOGENOM	HOG000232011.
HOVERGEN	HBG025852.
InParanoid	Q6P7Q4.
KO	K01759.
OMA	WALSRKA.
OrthoDB	EOG4TQMB3.
Enzyme and pathway databases
SABIO-RK	Q6P7Q4.
UniPathway	UPA00619; UER00675.
Gene expression databases
Genevestigator	Q6P7Q4.
GermOnline	ENSRNOG00000000541. Rattus norvegicus.
Family and domain databases
InterPro	IPR004360. Glyas_Fos-R_dOase_dom. IPR004361. Glyoxalase_1. IPR018146. Glyoxalase_1_CS. [Graphical view]
Pfam	PF00903. Glyoxalase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00068. glyox_I. 1 hit.
PROSITE	PS00934. GLYOXALASE_I_1. 1 hit. PS00935. GLYOXALASE_I_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL2306.
NextBio	637974.

Entry information

Entry name

LGUL_RAT

Accession

Primary (citable) accession number: Q6P7Q4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 80 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program