Reviewed, UniProtKB/Swiss-Prot Q6P7Q4 (LGUL_RAT)

Names and origin

Protein names

Recommended name:
    Lactoylglutathione lyase
    EC=4.4.1.5
Alternative name(s):
    Methylglyoxalase
    Aldoketomutase
    Glyoxalase I
      Short name=Glx I
    Ketone-aldehyde mutase
    S-D-lactoylglutathione methylglyoxal lyase

Gene names

Name:

Glo1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione By similarity.
Catalytic activity	(R)-S-lactoylglutathione = glutathione + methylglyoxal.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the glyoxalase I family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Lyase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	anti-apoptosis Inferred from sequence or structural similarity. Source: UniProtKB carbohydrate metabolic process Inferred from direct assay. Source: RGD glutathione metabolic process Inferred from direct assay. Source: RGD methylglyoxal metabolic process Inferred from direct assay. Source: RGD
Cellular component	soluble fraction Inferred from direct assay. Source: RGD
Molecular function	lactoylglutathione lyase activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 184	183	Lactoylglutathione lyase		PRO_0000168079
Sites
	Metal binding	34	1	Zinc By similarity
	Metal binding	100	1	Zinc By similarity
	Metal binding	127	1	Zinc By similarity
	Metal binding	173	1	Zinc By similarity
Amino acid modifications
	Modified residue	148	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6P7Q4-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 1834FA5E9871A1C7	FASTA	184	20,820
	10 20 30 40 50 60 MAEPQPASSG LTDEAALSCC SDPDPSTKDF LLQQTMLRIK DPKKSLDFYT RVLGLTLLQK 70 80 90 100 110 120 LDFPSMKFSL YFLAYEDKND IPKDKTERTA WAFSRKATLE LTHNWGTEDD ETQSYHNGNS 130 140 150 160 170 180 DPRGFGHIGI AVPDVYEACK RFEELGVKFV KKPDDGKMKG LAFVQDPDGY WIEILNPNKM ATII

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
[2]	Lubec G., Afjehi-Sadat L., Chen W.-Q. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 29-38; 44-83; 89-95 AND 124-148, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus and Spinal cord.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC061570 mRNA. Translation: AAH61570.1.
IPI	IPI00188304.
RefSeq	NP_997477.1.
UniGene	Rn.108014
3D structure databases
SMR	Q6P7Q4. Positions 1-183, 2-184.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6P7Q4.
Proteomic databases
PRIDE	Q6P7Q4.
Genome annotation databases
Ensembl	ENSRNOT00000000650; ENSRNOP00000000650; ENSRNOG00000000541; Rattus norvegicus. [Genome view]
GeneID	294320.
KEGG	rno:294320.
NMPDR	fig|10116.3.peg.17657.
UCSC	NM_207594. rat.
Organism-specific databases
CTD	294320.
RGD	2702. Glo1.
Phylogenomic databases
HOVERGEN	Q6P7Q4.
OMA	FAYHNGN.
Enzyme and pathway databases
BRENDA	4.4.1.5. 248.
Gene expression databases
ArrayExpress	Q6P7Q4.
Genevestigator	Q6P7Q4.
GermOnline	ENSRNOG00000000541. Rattus norvegicus.
Family and domain databases
InterPro	IPR004360. Glyas_bleo-R_dOase. IPR004361. Glyoxalase_1. IPR018146. Glyoxalase_1_CS. [Graphical view]
Pfam	PF00903. Glyoxalase. 1 hit. [Graphical view]
ProDom	PD002334. Gly_diox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00068. glyox_I. 1 hit.
PROSITE	PS00934. GLYOXALASE_I_1. 1 hit. PS00935. GLYOXALASE_I_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	637974.

Entry information

Entry name

LGUL_RAT

Accession

Primary (citable) accession number: Q6P7Q4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 52 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)