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Protein

Protrudin

Gene

Zfyve27

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Key regulator of RAB11-dependent vesicular trafficking during neurite extension through polarized membrane transport (PubMed:17082457). Promotes axonal elongation and contributes to the establishment of neuronal cell polarity. Involved in nerve growth factor-induced neurite formation in VAPA-dependent manner. Contributes to both the formation and stabilization of the tubular ER network. Involved in ER morphogenesis by regulating the sheet-to-tubule balance and possibly the density of tubule interconnections. Acts as an adapter protein that facilitates the interaction of KIF5A with VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 and the ZFYVE27-KIF5A complex contributes to the transport of these proteins in neurons. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a KIF5A/B-dependent manner (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri337 – 403FYVE-typePROSITE-ProRule annotationAdd BLAST67

GO - Molecular functioni

GO - Biological processi

  • endoplasmic reticulum tubular network assembly Source: UniProtKB
  • neuron projection development Source: UniProtKB
  • neurotrophin TRK receptor signaling pathway Source: UniProtKB
  • positive regulation of axon extension Source: UniProtKB
  • protein localization to plasma membrane Source: UniProtKB
  • vesicle-mediated transport Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protrudin
Alternative name(s):
Zinc finger FYVE domain-containing protein 27
Gene namesi
Name:Zfyve27
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi735177. Zfyve27.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 63CytoplasmicBy similarityAdd BLAST63
Transmembranei64 – 85HelicalSequence analysisAdd BLAST22
Topological domaini86 – 90LumenalBy similarity5
Transmembranei91 – 109HelicalSequence analysisAdd BLAST19
Topological domaini110 – 187CytoplasmicBy similarityAdd BLAST78
Transmembranei188 – 208HelicalSequence analysisAdd BLAST21
Topological domaini209 – 404ExtracellularBy similarityAdd BLAST196

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Endoplasmic reticulum, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004103501 – 404ProtrudinAdd BLAST404

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi209N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Phosphorylated. Phosphorylation is induced by NGF through the MAPK/ERK pathway and modulates interaction with RAB11A (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ6P7B7.
PRIDEiQ6P7B7.

Expressioni

Gene expression databases

BgeeiENSRNOG00000014903.
ExpressionAtlasiQ6P7B7. baseline and differential.

Interactioni

Subunit structurei

Can form homooligomers (monomers, dimers and tetramers). Interacts with FKBP8; may negatively regulate ZFYVE27 phosphorylation. Interacts with VAPA (via MSP domain); may regulate ZFYVE27 retention in the endoplasmic reticulum and its function in cell projections formation. Interacts with VAPB (via MSP domain) (By similarity). Interacts with RAB11A (GDP-bound form); regulates RAB11A (PubMed:17082457). Interacts with RAB11B (GDP-bound form), REEP1, REEP5, ATL1, ATL2, ATL3, SPAST, SURF4, KIF5A, KIF5B, KIF5C and RTN3 (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ6P7B7. 1 interactor.
STRINGi10116.ENSRNOP00000051399.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 205Sufficient for localization to endoplasmic reticulum tubular network and for interactions with REEP1, REEP5, ATL1, ATL2, ATL3 and SPASTBy similarityAdd BLAST205
Regioni1 – 92Sufficient for homooligomerizationBy similarityAdd BLAST92
Regioni51 – 64Necessary for interaction with RAB11A and function in neurite outgrowthBy similarityAdd BLAST14
Regioni271 – 354Necessary for interaction with KIF5ABy similarityAdd BLAST84
Regioni286 – 292Necessary for interaction with VAPABy similarity7

Sequence similaritiesi

Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri337 – 403FYVE-typePROSITE-ProRule annotationAdd BLAST67

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IJBT. Eukaryota.
ENOG4111KCR. LUCA.
GeneTreeiENSGT00390000013298.
HOGENOMiHOG000155785.
HOVERGENiHBG054907.
InParanoidiQ6P7B7.
KOiK19368.
OrthoDBiEOG091G0QCA.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6P7B7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSSDRDLSG PEASPSVMPE VLSECSPAPT KSTAFDLFNL VLSYKRLEIY
60 70 80 90 100
LEPLKDAGDG VRYLLRWQMP LCSLLTCLGL NILFLTLNEG AWYSVGALII
110 120 130 140 150
SVPALLGYLQ EVCRAQLPES ELMRRKYHSV RQEDLQRVRL SRPEAVAEVK
160 170 180 190 200
SFLIRLEAFL ARLCYTCESA YRVLHWENPV VSSQFYGALL GMVCMLYLLP
210 220 230 240 250
LCWVLALLNS TLFLGNGEFF RVVSEYRACL QRRMSPKQEE CVCEGSALQD
260 270 280 290 300
AGGRAVVLDS TPAPTPTEDL TPGSVEEAEE AEPDEEFKDA IEEDDEGTPC
310 320 330 340 350
PAEDELTMQD NGFLSKNEVL RSKVSKLTER LRKRYPTNNF GNCAGCAATF
360 370 380 390 400
SVLKKRRSCS NCGNSFCSRC CSFKVPKSSM GATAPEAQRE TVFVCASCNQ

TLSK
Length:404
Mass (Da):44,948
Last modified:June 28, 2011 - v2
Checksum:i6D63A2ECB90264FC
GO

Sequence cautioni

The sequence AAH61741 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC061741 mRNA. Translation: AAH61741.1. Different initiation.
RefSeqiNP_954535.2. NM_199104.2.
UniGeneiRn.8961.

Genome annotation databases

EnsembliENSRNOT00000083287; ENSRNOP00000074613; ENSRNOG00000014903.
GeneIDi309376.
KEGGirno:309376.
UCSCiRGD:735177. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC061741 mRNA. Translation: AAH61741.1. Different initiation.
RefSeqiNP_954535.2. NM_199104.2.
UniGeneiRn.8961.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6P7B7. 1 interactor.
STRINGi10116.ENSRNOP00000051399.

Proteomic databases

PaxDbiQ6P7B7.
PRIDEiQ6P7B7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000083287; ENSRNOP00000074613; ENSRNOG00000014903.
GeneIDi309376.
KEGGirno:309376.
UCSCiRGD:735177. rat.

Organism-specific databases

CTDi118813.
RGDi735177. Zfyve27.

Phylogenomic databases

eggNOGiENOG410IJBT. Eukaryota.
ENOG4111KCR. LUCA.
GeneTreeiENSGT00390000013298.
HOGENOMiHOG000155785.
HOVERGENiHBG054907.
InParanoidiQ6P7B7.
KOiK19368.
OrthoDBiEOG091G0QCA.

Miscellaneous databases

PROiQ6P7B7.

Gene expression databases

BgeeiENSRNOG00000014903.
ExpressionAtlasiQ6P7B7. baseline and differential.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZFY27_RAT
AccessioniPrimary (citable) accession number: Q6P7B7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: November 30, 2016
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.