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Protein

Lysosomal alpha-glucosidase

Gene

Gaa

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Essential for the degradation of glygogen to glucose in lysosomes.By similarity

Catalytic activityi

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei518 – 5181NucleophilePROSITE-ProRule annotation
Active sitei521 – 5211By similarity

GO - Molecular functioni

  • alpha-1,4-glucosidase activity Source: RGD
  • carbohydrate binding Source: RGD
  • maltose alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  • glycogen catabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

SABIO-RKQ6P7A9.

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal alpha-glucosidase (EC:3.2.1.20)
Alternative name(s):
Acid maltase
Gene namesi
Name:Gaa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi735227. Gaa.

Subcellular locationi

GO - Cellular componenti

  • lysosomal membrane Source: UniProtKB-SubCell
  • lysosome Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Propeptidei28 – 6942By similarityPRO_0000260442Add
BLAST
Chaini70 – 953884Lysosomal alpha-glucosidasePRO_0000260443Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi140 – 1401N-linked (GlcNAc...)Sequence Analysis
Glycosylationi233 – 2331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi390 – 3901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi470 – 4701N-linked (GlcNAc...)Sequence Analysis
Glycosylationi652 – 6521N-linked (GlcNAc...)Sequence Analysis
Glycosylationi883 – 8831N-linked (GlcNAc...)Sequence Analysis
Glycosylationi926 – 9261N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ6P7A9.

Expressioni

Gene expression databases

GenevisibleiQ6P7A9. RN.

Interactioni

Protein-protein interaction databases

BioGridi266559. 1 interaction.
IntActiQ6P7A9. 1 interaction.
STRINGi10116.ENSRNOP00000064500.

Structurei

3D structure databases

ProteinModelPortaliQ6P7A9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 13152P-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 31 family.Curated
Contains 1 P-type (trefoil) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG006297.
InParanoidiQ6P7A9.
KOiK12316.
PhylomeDBiQ6P7A9.

Family and domain databases

Gene3Di4.10.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR030458. Glyco_hydro_31_AS.
IPR030459. Glyco_hydro_31_CS.
IPR025887. Glyco_hydro_31_N_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR000519. P_trefoil.
IPR017957. P_trefoil_CS.
[Graphical view]
PfamiPF13802. Gal_mutarotas_2. 1 hit.
PF01055. Glyco_hydro_31. 1 hit.
PF00088. Trefoil. 1 hit.
[Graphical view]
SMARTiSM00018. PD. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
PS00025. P_TREFOIL_1. 1 hit.
PS51448. P_TREFOIL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6P7A9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIRKPLCSN SVVGACTLVS LTTAVILGHL MLRELMLLPQ DLHESSSGLW
60 70 80 90 100
KTYRPHHQES YEPAPLHIQE HAEQLRAVPT QCDVTPNSRF DCAPDKGITQ
110 120 130 140 150
EQCEARGCCW VPAGQVLNGP VMGQPWCFFP PSYPSYRLEN LSSTESGYTA
160 170 180 190 200
TLTRTSPTFF PKDVLTLQLE VLMETDSRLH FMIKDPTSKR YEVPLETPRV
210 220 230 240 250
LSQAPSPLYS VEFSEEPFGV IVRRKLGGRV LLNTTVAPLF FADQFLQLST
260 270 280 290 300
SLPSQHIAGL GEHLSPLMLS TEWTRITLWN RDVAPSQGVN LYGSHPFYLA
310 320 330 340 350
LEDGGLAHGV FLLNSNAMDV VLQPSPALTW RSTGGILDVY VFLGPEPKSV
360 370 380 390 400
VQQYLDVVGY PFMPPYWGLG FHLCRWGYSS TAIVRQVVEN MTRTHFPLDV
410 420 430 440 450
QWNDLDYMDA RRDFTFNQDG FADFPDMVHE LHQGGRRYMM IVDPAISSSG
460 470 480 490 500
PAGSYRPYDE GLRRGVFITN ETGQPLIGKV WPGSTAFPDF TNPETLDWWQ
510 520 530 540 550
DMVSEFHAQV PFDGMWIDMN EPSNFIRGSQ QGCPDNELEN PPYVPGVVGG
560 570 580 590 600
ALQAATICAS SHQFLSTHYN LHNLYGLTEA IASSRALVKT RGTRPFVISR
610 620 630 640 650
STFAGHGRYA GHWTGDVWSS WEHLAYSVPE ILQFNLLGVP LVGADICGFQ
660 670 680 690 700
GNTTEELCVR WTQLGAFYPF MRNHNDLNSL PQEPYRFSET AQQAMRKAFT
710 720 730 740 750
LRYALLPYLY TLFHGAHVKG DTVARPLFLE FPEDPSTWSV DRQLLWGPAL
760 770 780 790 800
LITPVLEPGK TDVTGYFPKG MWYNLQMVPV ETLGSLPSSS PASSFRSIVH
810 820 830 840 850
SKGQWLTLEA PLDTINVHLR AGYIIPLQGP SLTTTESRKQ PMALAVALTE
860 870 880 890 900
SGEASGELFW DDGESLGVLE RGAYTLVTFS AKNNTIVNKL VHVTKEGGEL
910 920 930 940 950
QLREVTILGV TTAPTQVLSN GISVSNFTYS PDDKSLSIPV SLLMGERFQI

DWS
Length:953
Mass (Da):106,207
Last modified:July 5, 2004 - v1
Checksum:iD99723779AD41E05
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC061753 mRNA. Translation: AAH61753.1.
RefSeqiNP_954549.1. NM_199118.1.
UniGeneiRn.162368.

Genome annotation databases

GeneIDi367562.
KEGGirno:367562.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC061753 mRNA. Translation: AAH61753.1.
RefSeqiNP_954549.1. NM_199118.1.
UniGeneiRn.162368.

3D structure databases

ProteinModelPortaliQ6P7A9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi266559. 1 interaction.
IntActiQ6P7A9. 1 interaction.
STRINGi10116.ENSRNOP00000064500.

Chemistry

BindingDBiQ6P7A9.
ChEMBLiCHEMBL3513.

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Proteomic databases

PRIDEiQ6P7A9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi367562.
KEGGirno:367562.

Organism-specific databases

CTDi2548.
RGDi735227. Gaa.

Phylogenomic databases

HOVERGENiHBG006297.
InParanoidiQ6P7A9.
KOiK12316.
PhylomeDBiQ6P7A9.

Enzyme and pathway databases

SABIO-RKQ6P7A9.

Miscellaneous databases

NextBioi691355.
PROiQ6P7A9.

Gene expression databases

GenevisibleiQ6P7A9. RN.

Family and domain databases

Gene3Di4.10.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR030458. Glyco_hydro_31_AS.
IPR030459. Glyco_hydro_31_CS.
IPR025887. Glyco_hydro_31_N_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR000519. P_trefoil.
IPR017957. P_trefoil_CS.
[Graphical view]
PfamiPF13802. Gal_mutarotas_2. 1 hit.
PF01055. Glyco_hydro_31. 1 hit.
PF00088. Trefoil. 1 hit.
[Graphical view]
SMARTiSM00018. PD. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
PS00025. P_TREFOIL_1. 1 hit.
PS51448. P_TREFOIL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.

Entry informationi

Entry nameiLYAG_RAT
AccessioniPrimary (citable) accession number: Q6P7A9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: July 5, 2004
Last modified: June 24, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.