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Protein

Lysosomal alpha-glucosidase

Gene

Gaa

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Essential for the degradation of glycogen in lysosomes. Has highest activity on alpha-1,4-linked glycosidic linkages, but can also hydrolyze alpha-1,6-linked glucans.By similarity

Catalytic activityi

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei404SubstrateBy similarity1
Active sitei518NucleophilePROSITE-ProRule annotation1
Active sitei521By similarity1
Binding sitei600SubstrateBy similarity1
Binding sitei616SubstrateBy similarity1
Binding sitei674SubstrateBy similarity1

GO - Molecular functioni

  • alpha-1,4-glucosidase activity Source: RGD
  • carbohydrate binding Source: RGD
  • maltose alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  • glycogen catabolic process Source: RGD

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

SABIO-RKiQ6P7A9

Protein family/group databases

CAZyiGH31 Glycoside Hydrolase Family 31

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal alpha-glucosidase (EC:3.2.1.20By similarity)
Alternative name(s):
Acid maltase
Gene namesi
Name:Gaa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi735227 Gaa

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3513

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
PropeptideiPRO_000026044228 – 69By similarityAdd BLAST42
ChainiPRO_000026044370 – 953Lysosomal alpha-glucosidaseAdd BLAST884

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi82 ↔ 109PROSITE-ProRule annotation
Disulfide bondi92 ↔ 108PROSITE-ProRule annotation
Disulfide bondi103 ↔ 127PROSITE-ProRule annotation
Glycosylationi140N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi233N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi390N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi470N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi533 ↔ 558By similarity
Disulfide bondi647 ↔ 658By similarity
Glycosylationi652N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi883N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi926N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ6P7A9
PRIDEiQ6P7A9

PTM databases

iPTMnetiQ6P7A9
PhosphoSitePlusiQ6P7A9
UniCarbKBiQ6P7A9

Interactioni

Protein-protein interaction databases

BioGridi266559, 1 interactor
IntActiQ6P7A9, 1 interactor
STRINGi10116.ENSRNOP00000064500

Chemistry databases

BindingDBiQ6P7A9

Structurei

3D structure databases

ProteinModelPortaliQ6P7A9
SMRiQ6P7A9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini80 – 131P-typePROSITE-ProRule annotationAdd BLAST52

Sequence similaritiesi

Belongs to the glycosyl hydrolase 31 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1065 Eukaryota
COG1501 LUCA
HOVERGENiHBG006297
InParanoidiQ6P7A9
KOiK12316
PhylomeDBiQ6P7A9

Family and domain databases

CDDicd00111 Trefoil, 1 hit
Gene3Di2.60.40.1180, 2 hits
InterProiView protein in InterPro
IPR031727 Gal_mutarotase_N
IPR011013 Gal_mutarotase_sf_dom
IPR000322 Glyco_hydro_31
IPR030458 Glyco_hydro_31_AS
IPR030459 Glyco_hydro_31_CS
IPR025887 Glyco_hydro_31_N_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR017957 P_trefoil_CS
IPR000519 P_trefoil_dom
PfamiView protein in Pfam
PF13802 Gal_mutarotas_2, 1 hit
PF01055 Glyco_hydro_31, 1 hit
PF16863 NtCtMGAM_N, 1 hit
PF00088 Trefoil, 1 hit
SMARTiView protein in SMART
SM00018 PD, 1 hit
SUPFAMiSSF51445 SSF51445, 2 hits
SSF74650 SSF74650, 1 hit
PROSITEiView protein in PROSITE
PS00129 GLYCOSYL_HYDROL_F31_1, 1 hit
PS00707 GLYCOSYL_HYDROL_F31_2, 1 hit
PS00025 P_TREFOIL_1, 1 hit
PS51448 P_TREFOIL_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6P7A9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIRKPLCSN SVVGACTLVS LTTAVILGHL MLRELMLLPQ DLHESSSGLW
60 70 80 90 100
KTYRPHHQES YEPAPLHIQE HAEQLRAVPT QCDVTPNSRF DCAPDKGITQ
110 120 130 140 150
EQCEARGCCW VPAGQVLNGP VMGQPWCFFP PSYPSYRLEN LSSTESGYTA
160 170 180 190 200
TLTRTSPTFF PKDVLTLQLE VLMETDSRLH FMIKDPTSKR YEVPLETPRV
210 220 230 240 250
LSQAPSPLYS VEFSEEPFGV IVRRKLGGRV LLNTTVAPLF FADQFLQLST
260 270 280 290 300
SLPSQHIAGL GEHLSPLMLS TEWTRITLWN RDVAPSQGVN LYGSHPFYLA
310 320 330 340 350
LEDGGLAHGV FLLNSNAMDV VLQPSPALTW RSTGGILDVY VFLGPEPKSV
360 370 380 390 400
VQQYLDVVGY PFMPPYWGLG FHLCRWGYSS TAIVRQVVEN MTRTHFPLDV
410 420 430 440 450
QWNDLDYMDA RRDFTFNQDG FADFPDMVHE LHQGGRRYMM IVDPAISSSG
460 470 480 490 500
PAGSYRPYDE GLRRGVFITN ETGQPLIGKV WPGSTAFPDF TNPETLDWWQ
510 520 530 540 550
DMVSEFHAQV PFDGMWIDMN EPSNFIRGSQ QGCPDNELEN PPYVPGVVGG
560 570 580 590 600
ALQAATICAS SHQFLSTHYN LHNLYGLTEA IASSRALVKT RGTRPFVISR
610 620 630 640 650
STFAGHGRYA GHWTGDVWSS WEHLAYSVPE ILQFNLLGVP LVGADICGFQ
660 670 680 690 700
GNTTEELCVR WTQLGAFYPF MRNHNDLNSL PQEPYRFSET AQQAMRKAFT
710 720 730 740 750
LRYALLPYLY TLFHGAHVKG DTVARPLFLE FPEDPSTWSV DRQLLWGPAL
760 770 780 790 800
LITPVLEPGK TDVTGYFPKG MWYNLQMVPV ETLGSLPSSS PASSFRSIVH
810 820 830 840 850
SKGQWLTLEA PLDTINVHLR AGYIIPLQGP SLTTTESRKQ PMALAVALTE
860 870 880 890 900
SGEASGELFW DDGESLGVLE RGAYTLVTFS AKNNTIVNKL VHVTKEGGEL
910 920 930 940 950
QLREVTILGV TTAPTQVLSN GISVSNFTYS PDDKSLSIPV SLLMGERFQI

DWS
Length:953
Mass (Da):106,207
Last modified:July 5, 2004 - v1
Checksum:iD99723779AD41E05
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC061753 mRNA Translation: AAH61753.1
RefSeqiNP_954549.1, NM_199118.1
UniGeneiRn.162368

Genome annotation databases

GeneIDi367562
KEGGirno:367562

Similar proteinsi

Entry informationi

Entry nameiLYAG_RAT
AccessioniPrimary (citable) accession number: Q6P7A9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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