Reviewed, UniProtKB/Swiss-Prot Q6P7A9 (LYAG_RAT)

Names and origin

Protein names

Recommended name:
    Lysosomal alpha-glucosidase
    EC=3.2.1.20
Alternative name(s):
    Acid maltase

Gene names

Name:

Gaa

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	953 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Essential for the degradation of glygogen to glucose in lysosomes By similarity.
Catalytic activity	Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 31 family. Contains 1 P-type (trefoil) domain.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	glycogen catabolic process Inferred from direct assay. Source: RGD
Cellular component	lysosome Inferred from direct assay. Source: RGD soluble fraction Inferred from direct assay. Source: RGD
Molecular function	alpha-glucosidase activity Inferred from direct assay. Source: RGD sugar binding Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Propeptide	28 – 69	42	By similarity		PRO_0000260442
	Chain	70 – 953	884	Lysosomal alpha-glucosidase		PRO_0000260443
Regions
	Domain	80 – 131	52	P-type
Sites
	Active site	518	1	Nucleophile By similarity
	Active site	521	1	By similarity
Amino acid modifications
	Glycosylation	140	1	N-linked (GlcNAc...) Potential
	Glycosylation	233	1	N-linked (GlcNAc...) Potential
	Glycosylation	390	1	N-linked (GlcNAc...) Potential
	Glycosylation	470	1	N-linked (GlcNAc...) Potential
	Glycosylation	652	1	N-linked (GlcNAc...) Potential
	Glycosylation	883	1	N-linked (GlcNAc...) Potential
	Glycosylation	926	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6P7A9-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: D99723779AD41E05	FASTA	953	106,207
	10 20 30 40 50 60 MNIRKPLCSN SVVGACTLVS LTTAVILGHL MLRELMLLPQ DLHESSSGLW KTYRPHHQES 70 80 90 100 110 120 YEPAPLHIQE HAEQLRAVPT QCDVTPNSRF DCAPDKGITQ EQCEARGCCW VPAGQVLNGP 130 140 150 160 170 180 VMGQPWCFFP PSYPSYRLEN LSSTESGYTA TLTRTSPTFF PKDVLTLQLE VLMETDSRLH 190 200 210 220 230 240 FMIKDPTSKR YEVPLETPRV LSQAPSPLYS VEFSEEPFGV IVRRKLGGRV LLNTTVAPLF 250 260 270 280 290 300 FADQFLQLST SLPSQHIAGL GEHLSPLMLS TEWTRITLWN RDVAPSQGVN LYGSHPFYLA 310 320 330 340 350 360 LEDGGLAHGV FLLNSNAMDV VLQPSPALTW RSTGGILDVY VFLGPEPKSV VQQYLDVVGY 370 380 390 400 410 420 PFMPPYWGLG FHLCRWGYSS TAIVRQVVEN MTRTHFPLDV QWNDLDYMDA RRDFTFNQDG 430 440 450 460 470 480 FADFPDMVHE LHQGGRRYMM IVDPAISSSG PAGSYRPYDE GLRRGVFITN ETGQPLIGKV 490 500 510 520 530 540 WPGSTAFPDF TNPETLDWWQ DMVSEFHAQV PFDGMWIDMN EPSNFIRGSQ QGCPDNELEN 550 560 570 580 590 600 PPYVPGVVGG ALQAATICAS SHQFLSTHYN LHNLYGLTEA IASSRALVKT RGTRPFVISR 610 620 630 640 650 660 STFAGHGRYA GHWTGDVWSS WEHLAYSVPE ILQFNLLGVP LVGADICGFQ GNTTEELCVR 670 680 690 700 710 720 WTQLGAFYPF MRNHNDLNSL PQEPYRFSET AQQAMRKAFT LRYALLPYLY TLFHGAHVKG 730 740 750 760 770 780 DTVARPLFLE FPEDPSTWSV DRQLLWGPAL LITPVLEPGK TDVTGYFPKG MWYNLQMVPV 790 800 810 820 830 840 ETLGSLPSSS PASSFRSIVH SKGQWLTLEA PLDTINVHLR AGYIIPLQGP SLTTTESRKQ 850 860 870 880 890 900 PMALAVALTE SGEASGELFW DDGESLGVLE RGAYTLVTFS AKNNTIVNKL VHVTKEGGEL 910 920 930 940 950 QLREVTILGV TTAPTQVLSN GISVSNFTYS PDDKSLSIPV SLLMGERFQI DWS

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC061753 mRNA. Translation: AAH61753.1.
IPI	IPI00400579.
RefSeq	NP_954549.1.
UniGene	Rn.162368
3D structure databases
HSSP	HSSP built from PDB template 1HI7 based on UniProtKB P04155.
SMR	Q6P7A9. Positions 85-953.
ModBase	Search...
Protein family/group databases
CAZy	GH31. Glycoside Hydrolase Family 31.
Proteomic databases
PRIDE	Q6P7A9.
Genome annotation databases
GeneID	367562.
KEGG	rno:367562.
Organism-specific databases
CTD	367562.
RGD	735227. Gaa.
Phylogenomic databases
eggNOG	maNOG15498.
HOVERGEN	Q6P7A9.
PhylomeDB	Q6P7A9.
Enzyme and pathway databases
BRENDA	3.2.1.20. 248.
Gene expression databases
Genevestigator	Q6P7A9.
Family and domain databases
InterPro	IPR000322. Glyco_hydro_31. IPR017853. Glyco_hydro_catalytic_core. IPR000519. P_trefoil. IPR017957. P_trefoil_CS. [Graphical view]
PANTHER	PTHR22762. Glyco_hydro_31. 1 hit.
Pfam	PF01055. Glyco_hydro_31. 1 hit. PF00088. Trefoil. 1 hit. [Graphical view]
SMART	SM00018. PD. 1 hit. [Graphical view]
PROSITE	PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit. PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit. PS00025. P_TREFOIL_1. 1 hit. PS51448. P_TREFOIL_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	691355.

Entry information

Entry name

LYAG_RAT

Accession

Primary (citable) accession number: Q6P7A9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	July 5, 2004
Last modified:	January 19, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)