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Protein

LARGE xylosyl- and glucuronyltransferase 2

Gene

Large2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide. Has a higher activity toward alpha-dystroglycan than LARGE.By similarity

Cofactori

Mn2+By similarityNote: Binds 2 Mn2+ ions per subunit. The xylosyltransferase part binds one Mn2+ and the beta-1,3-glucuronyltransferase part binds one Mn2+.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi171Manganese 1By similarity1
Metal bindingi173Manganese 1By similarity1
Metal bindingi491Manganese 2By similarity1
Metal bindingi493Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-5173105. O-linked glycosylation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT49. Glycosyltransferase Family 49.
GT8. Glycosyltransferase Family 8.

Names & Taxonomyi

Protein namesi
Recommended name:
LARGE xylosyl- and glucuronyltransferase 2Imported (EC:2.4.-.-)
Alternative name(s):
Glycosyltransferase-like 1B
Including the following 2 domains:
Xylosyltransferase LARGE2Curated (EC:2.4.2.-)
Beta-1,3-glucuronyltransferase LARGE2Curated (EC:2.4.1.-)
Gene namesi
Name:Large2Imported
Synonyms:Gyltl1b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi735214. Large2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 28Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini29 – 690LumenalSequence analysisAdd BLAST662

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002268131 – 690LARGE xylosyl- and glucuronyltransferase 2Add BLAST690

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi50N-linked (GlcNAc...)Sequence analysis1
Glycosylationi77N-linked (GlcNAc...)Sequence analysis1
Glycosylationi201N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ6P7A1.
PRIDEiQ6P7A1.

Expressioni

Gene expression databases

BgeeiENSRNOG00000006353.
GenevisibleiQ6P7A1. RN.

Interactioni

Subunit structurei

Interacts with B3GNT1/B4GAT1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000041797.

Structurei

3D structure databases

ProteinModelPortaliQ6P7A1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni67 – 342Xylosyltransferase activityBy similarityAdd BLAST276
Regioni343 – 686Glucuronyltransferase activityBy similarityAdd BLAST344

Sequence similaritiesi

In the C-terminal section; belongs to the glycosyltransferase 49 family.Curated
In the N-terminal section; belongs to the glycosyltransferase 8 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3765. Eukaryota.
ENOG410XRNY. LUCA.
GeneTreeiENSGT00530000063165.
HOGENOMiHOG000231467.
HOVERGENiHBG052308.
InParanoidiQ6P7A1.
KOiK09668.
OMAiPQPHDVT.
OrthoDBiEOG091G034V.
PhylomeDBiQ6P7A1.
TreeFamiTF319168.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR002495. Glyco_trans_8.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01501. Glyco_transf_8. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6P7A1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPRGRPRAL GAALLLLLLL VVGFFLFGRD PEYGLGTTAT LDGDPYGSRN
60 70 80 90 100
RSTSSLQLLL PPKCEMLHVA IVCAGYNSSR EIITLMKSVL FYRKNPLHLH
110 120 130 140 150
LITDAVARNI LETLFRTWMV PAVVVSFYDA EELKPLVSWI PNKHYSGLYG
160 170 180 190 200
LMKLVLPSVL PLSLARVIVL DTDVTFSSDI MELWALFGHF SDKQVVGLVE
210 220 230 240 250
NQSDWYLGNL WKNHRPWPAL GRGFNTGVIL LWLDRLQQIG WEQMWKLTAK
260 270 280 290 300
RELLTLTATS LADQDIFNAV IKEHPELVHP LPCVWNVQLS DHTLAERCYL
310 320 330 340 350
EAADLKVIHW NSPKKLRVKN KHAEFFRDLH LTFLGFDGKL LCRELFGCPN
360 370 380 390 400
QFPPGVEQLQ QALAQLDEEE PCFEFRQQQL TVHRVHITFL SHQPPPPRPH
410 420 430 440 450
DVTLVAQLSM DRLQMLEALC RHWRGPMSLA LYLTDAEAQQ FLRFVETSPV
460 470 480 490 500
LSARKDVAYH VVYRDGPLYP VNQLRNVALA QALTPYVFLS DIDFLPAYSL
510 520 530 540 550
YDYLRASIEQ LALGRRQRKA ALVVPAFETL HYRFSFPNSK AELLTLLDAG
560 570 580 590 600
SLHTFRYHEW PQGHASTDYT RWREAQAPYR VQWSADYEPY VVVPRDCPRY
610 620 630 640 650
DPRFVGFGWN KVAHIIELDA QEYEFLVLPE AFSIHLPHAP SLDISRFRSS
660 670 680 690
PTYRDCLQAL KEEFHQDLSR RYGSAALKYL TALQQSRSRA
Length:690
Mass (Da):79,309
Last modified:July 5, 2004 - v1
Checksum:i79C609E33EDD33E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC061762 mRNA. Translation: AAH61762.1.
RefSeqiNP_954538.1. NM_199107.1.
UniGeneiRn.205174.

Genome annotation databases

EnsembliENSRNOT00000051258; ENSRNOP00000041797; ENSRNOG00000006353.
GeneIDi311202.
KEGGirno:311202.
UCSCiRGD:735214. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC061762 mRNA. Translation: AAH61762.1.
RefSeqiNP_954538.1. NM_199107.1.
UniGeneiRn.205174.

3D structure databases

ProteinModelPortaliQ6P7A1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000041797.

Protein family/group databases

CAZyiGT49. Glycosyltransferase Family 49.
GT8. Glycosyltransferase Family 8.

Proteomic databases

PaxDbiQ6P7A1.
PRIDEiQ6P7A1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000051258; ENSRNOP00000041797; ENSRNOG00000006353.
GeneIDi311202.
KEGGirno:311202.
UCSCiRGD:735214. rat.

Organism-specific databases

CTDi120071.
RGDi735214. Large2.

Phylogenomic databases

eggNOGiKOG3765. Eukaryota.
ENOG410XRNY. LUCA.
GeneTreeiENSGT00530000063165.
HOGENOMiHOG000231467.
HOVERGENiHBG052308.
InParanoidiQ6P7A1.
KOiK09668.
OMAiPQPHDVT.
OrthoDBiEOG091G034V.
PhylomeDBiQ6P7A1.
TreeFamiTF319168.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-RNO-5173105. O-linked glycosylation.

Miscellaneous databases

PROiQ6P7A1.

Gene expression databases

BgeeiENSRNOG00000006353.
GenevisibleiQ6P7A1. RN.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR002495. Glyco_trans_8.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01501. Glyco_transf_8. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLARG2_RAT
AccessioniPrimary (citable) accession number: Q6P7A1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.