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Protein

Glycosyltransferase-like protein LARGE2

Gene

Gyltl1b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide. Has a higher activity toward alpha-dystroglycan than LARGE.By similarity

Cofactori

Mn2+By similarityNote: Binds 2 Mn2+ ions per subunit. The xylosyltransferase part binds one Mn2+ and the beta-1,3-glucuronyltransferase part binds one Mn2+.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi171 – 1711Manganese 1By similarity
Metal bindingi173 – 1731Manganese 1By similarity
Metal bindingi491 – 4911Manganese 2By similarity
Metal bindingi493 – 4931Manganese 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-5173105. O-linked glycosylation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT49. Glycosyltransferase Family 49.
GT8. Glycosyltransferase Family 8.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycosyltransferase-like protein LARGE2 (EC:2.4.-.-)
Alternative name(s):
Glycosyltransferase-like 1B
Including the following 2 domains:
Xylosyltransferase LARGE2Curated (EC:2.4.2.-)
Beta-1,3-glucuronyltransferase LARGE2Curated (EC:2.4.1.-)
Gene namesi
Name:Gyltl1b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi735214. Gyltl1b.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence analysis
Transmembranei8 – 2821Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini29 – 690662LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 690690Glycosyltransferase-like protein LARGE2PRO_0000226813Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi50 – 501N-linked (GlcNAc...)Sequence analysis
Glycosylationi77 – 771N-linked (GlcNAc...)Sequence analysis
Glycosylationi201 – 2011N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ6P7A1.
PRIDEiQ6P7A1.

Expressioni

Gene expression databases

GenevisibleiQ6P7A1. RN.

Interactioni

Subunit structurei

Interacts with B3GNT1/B4GAT1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000041797.

Structurei

3D structure databases

ProteinModelPortaliQ6P7A1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni67 – 342276Xylosyltransferase activityBy similarityAdd
BLAST
Regioni343 – 686344Glucuronyltransferase activityBy similarityAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the glycosyltransferase 49 family.Curated
In the N-terminal section; belongs to the glycosyltransferase 8 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3765. Eukaryota.
ENOG410XRNY. LUCA.
GeneTreeiENSGT00530000063165.
HOGENOMiHOG000231467.
HOVERGENiHBG052308.
InParanoidiQ6P7A1.
KOiK09668.
OMAiAPGCFPG.
OrthoDBiEOG7RJPQV.
PhylomeDBiQ6P7A1.
TreeFamiTF319168.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR002495. Glyco_trans_8.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01501. Glyco_transf_8. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6P7A1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPRGRPRAL GAALLLLLLL VVGFFLFGRD PEYGLGTTAT LDGDPYGSRN
60 70 80 90 100
RSTSSLQLLL PPKCEMLHVA IVCAGYNSSR EIITLMKSVL FYRKNPLHLH
110 120 130 140 150
LITDAVARNI LETLFRTWMV PAVVVSFYDA EELKPLVSWI PNKHYSGLYG
160 170 180 190 200
LMKLVLPSVL PLSLARVIVL DTDVTFSSDI MELWALFGHF SDKQVVGLVE
210 220 230 240 250
NQSDWYLGNL WKNHRPWPAL GRGFNTGVIL LWLDRLQQIG WEQMWKLTAK
260 270 280 290 300
RELLTLTATS LADQDIFNAV IKEHPELVHP LPCVWNVQLS DHTLAERCYL
310 320 330 340 350
EAADLKVIHW NSPKKLRVKN KHAEFFRDLH LTFLGFDGKL LCRELFGCPN
360 370 380 390 400
QFPPGVEQLQ QALAQLDEEE PCFEFRQQQL TVHRVHITFL SHQPPPPRPH
410 420 430 440 450
DVTLVAQLSM DRLQMLEALC RHWRGPMSLA LYLTDAEAQQ FLRFVETSPV
460 470 480 490 500
LSARKDVAYH VVYRDGPLYP VNQLRNVALA QALTPYVFLS DIDFLPAYSL
510 520 530 540 550
YDYLRASIEQ LALGRRQRKA ALVVPAFETL HYRFSFPNSK AELLTLLDAG
560 570 580 590 600
SLHTFRYHEW PQGHASTDYT RWREAQAPYR VQWSADYEPY VVVPRDCPRY
610 620 630 640 650
DPRFVGFGWN KVAHIIELDA QEYEFLVLPE AFSIHLPHAP SLDISRFRSS
660 670 680 690
PTYRDCLQAL KEEFHQDLSR RYGSAALKYL TALQQSRSRA
Length:690
Mass (Da):79,309
Last modified:July 5, 2004 - v1
Checksum:i79C609E33EDD33E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC061762 mRNA. Translation: AAH61762.1.
RefSeqiNP_954538.1. NM_199107.1.
UniGeneiRn.205174.

Genome annotation databases

EnsembliENSRNOT00000051258; ENSRNOP00000041797; ENSRNOG00000006353.
GeneIDi311202.
KEGGirno:311202.
UCSCiRGD:735214. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC061762 mRNA. Translation: AAH61762.1.
RefSeqiNP_954538.1. NM_199107.1.
UniGeneiRn.205174.

3D structure databases

ProteinModelPortaliQ6P7A1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000041797.

Protein family/group databases

CAZyiGT49. Glycosyltransferase Family 49.
GT8. Glycosyltransferase Family 8.

Proteomic databases

PaxDbiQ6P7A1.
PRIDEiQ6P7A1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000051258; ENSRNOP00000041797; ENSRNOG00000006353.
GeneIDi311202.
KEGGirno:311202.
UCSCiRGD:735214. rat.

Organism-specific databases

CTDi120071.
RGDi735214. Gyltl1b.

Phylogenomic databases

eggNOGiKOG3765. Eukaryota.
ENOG410XRNY. LUCA.
GeneTreeiENSGT00530000063165.
HOGENOMiHOG000231467.
HOVERGENiHBG052308.
InParanoidiQ6P7A1.
KOiK09668.
OMAiAPGCFPG.
OrthoDBiEOG7RJPQV.
PhylomeDBiQ6P7A1.
TreeFamiTF319168.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-RNO-5173105. O-linked glycosylation.

Miscellaneous databases

PROiQ6P7A1.

Gene expression databases

GenevisibleiQ6P7A1. RN.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR002495. Glyco_trans_8.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01501. Glyco_transf_8. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.

Entry informationi

Entry nameiLARG2_RAT
AccessioniPrimary (citable) accession number: Q6P7A1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: July 5, 2004
Last modified: June 8, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.