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Protein

LARGE xylosyl- and glucuronyltransferase 2

Gene

Large2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide. Has a higher activity toward alpha-dystroglycan than LARGE.By similarity

Cofactori

Mn2+By similarityNote: Binds 2 Mn2+ ions per subunit. The xylosyltransferase part binds one Mn2+ and the beta-1,3-glucuronyltransferase part binds one Mn2+.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi171Manganese 1By similarity1
Metal bindingi173Manganese 1By similarity1
Metal bindingi491Manganese 2By similarity1
Metal bindingi493Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Multifunctional enzyme, Transferase
LigandManganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-5173105 O-linked glycosylation
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT49 Glycosyltransferase Family 49
GT8 Glycosyltransferase Family 8

Names & Taxonomyi

Protein namesi
Recommended name:
LARGE xylosyl- and glucuronyltransferase 2Imported (EC:2.4.-.-)
Alternative name(s):
Glycosyltransferase-like 1B
Including the following 2 domains:
Xylosyltransferase LARGE2Curated (EC:2.4.2.-)
Beta-1,3-glucuronyltransferase LARGE2Curated (EC:2.4.1.-)
Gene namesi
Name:Large2Imported
Synonyms:Gyltl1b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi735214 Large2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 28Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini29 – 690LumenalSequence analysisAdd BLAST662

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002268131 – 690LARGE xylosyl- and glucuronyltransferase 2Add BLAST690

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi50N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi77N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi201N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ6P7A1
PRIDEiQ6P7A1

Expressioni

Gene expression databases

BgeeiENSRNOG00000006353
GenevisibleiQ6P7A1 RN

Interactioni

Subunit structurei

Interacts with B3GNT1/B4GAT1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000041797

Structurei

3D structure databases

ProteinModelPortaliQ6P7A1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni67 – 342Xylosyltransferase activityBy similarityAdd BLAST276
Regioni343 – 686Glucuronyltransferase activityBy similarityAdd BLAST344

Sequence similaritiesi

In the C-terminal section; belongs to the glycosyltransferase 49 family.Curated
In the N-terminal section; belongs to the glycosyltransferase 8 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3765 Eukaryota
ENOG410XRNY LUCA
GeneTreeiENSGT00530000063165
HOGENOMiHOG000231467
HOVERGENiHBG052308
InParanoidiQ6P7A1
KOiK09668
OMAiLHYRFSF
OrthoDBiEOG091G034V
PhylomeDBiQ6P7A1
TreeFamiTF319168

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR002495 Glyco_trans_8
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF01501 Glyco_transf_8, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

Q6P7A1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPRGRPRAL GAALLLLLLL VVGFFLFGRD PEYGLGTTAT LDGDPYGSRN
60 70 80 90 100
RSTSSLQLLL PPKCEMLHVA IVCAGYNSSR EIITLMKSVL FYRKNPLHLH
110 120 130 140 150
LITDAVARNI LETLFRTWMV PAVVVSFYDA EELKPLVSWI PNKHYSGLYG
160 170 180 190 200
LMKLVLPSVL PLSLARVIVL DTDVTFSSDI MELWALFGHF SDKQVVGLVE
210 220 230 240 250
NQSDWYLGNL WKNHRPWPAL GRGFNTGVIL LWLDRLQQIG WEQMWKLTAK
260 270 280 290 300
RELLTLTATS LADQDIFNAV IKEHPELVHP LPCVWNVQLS DHTLAERCYL
310 320 330 340 350
EAADLKVIHW NSPKKLRVKN KHAEFFRDLH LTFLGFDGKL LCRELFGCPN
360 370 380 390 400
QFPPGVEQLQ QALAQLDEEE PCFEFRQQQL TVHRVHITFL SHQPPPPRPH
410 420 430 440 450
DVTLVAQLSM DRLQMLEALC RHWRGPMSLA LYLTDAEAQQ FLRFVETSPV
460 470 480 490 500
LSARKDVAYH VVYRDGPLYP VNQLRNVALA QALTPYVFLS DIDFLPAYSL
510 520 530 540 550
YDYLRASIEQ LALGRRQRKA ALVVPAFETL HYRFSFPNSK AELLTLLDAG
560 570 580 590 600
SLHTFRYHEW PQGHASTDYT RWREAQAPYR VQWSADYEPY VVVPRDCPRY
610 620 630 640 650
DPRFVGFGWN KVAHIIELDA QEYEFLVLPE AFSIHLPHAP SLDISRFRSS
660 670 680 690
PTYRDCLQAL KEEFHQDLSR RYGSAALKYL TALQQSRSRA
Length:690
Mass (Da):79,309
Last modified:July 5, 2004 - v1
Checksum:i79C609E33EDD33E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC061762 mRNA Translation: AAH61762.1
RefSeqiNP_954538.1, NM_199107.1
UniGeneiRn.205174

Genome annotation databases

EnsembliENSRNOT00000051258; ENSRNOP00000041797; ENSRNOG00000006353
GeneIDi311202
KEGGirno:311202
UCSCiRGD:735214 rat

Similar proteinsi

Entry informationi

Entry nameiLARG2_RAT
AccessioniPrimary (citable) accession number: Q6P7A1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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