Sars

Functionⁱ

Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction: serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser). Is probably also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec). In the nucleus, binds to the VEGFA core promoter and prevents MYC binding and transcriptional activation by MYC. Recruits SIRT2 to the VEGFA promoter, promoting deacetylation of histone H4 at 'Lys-16' (H4K16). Thereby, inhibits the production of VEGFA and sprouting angiogenesis mediated by VEGFA.By similarity

Catalytic activityⁱ

ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser).By similarity

ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec).By similarity

Pathwayⁱ: selenocysteinyl-tRNA(Sec) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-seryl-tRNA(Sec) from L-serine and tRNA(Sec).
Proteins known to be involved in this subpathway in this organism are:

Serine--tRNA ligase, cytoplasmic (Sars)

This subpathway is part of the pathway selenocysteinyl-tRNA(Sec) biosynthesis, which is itself part of Aminoacyl-tRNA biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-seryl-tRNA(Sec) from L-serine and tRNA(Sec), the pathway selenocysteinyl-tRNA(Sec) biosynthesis and in Aminoacyl-tRNA biosynthesis.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	271	L-serineBy similarity	1
Binding siteⁱ	302	L-serineBy similarity	1
Binding siteⁱ	325	L-serineBy similarity	1
Binding siteⁱ	427	L-serineBy similarity	1
Binding siteⁱ	429	Important for serine bindingBy similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Length
Nucleotide bindingⁱ	302 – 304	ATPBy similarity	3
Nucleotide bindingⁱ	318 – 321	ATPBy similarity	4
Nucleotide bindingⁱ	391 – 394	ATPBy similarity	4

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
core promoter sequence-specific DNA binding Source: UniProtKB
protein homodimerization activity
Source: RGD
Inferred from sequence orthologyⁱ
- 26433229
serine-tRNA ligase activity Source: UniProtKB

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

negative regulation of angiogenesis Source: UniProtKB
negative regulation of transcription by RNA polymerase II Source: UniProtKB
negative regulation of vascular endothelial growth factor production Source: UniProtKB
selenocysteinyl-tRNA(Sec) biosynthetic process Source: UniProtKB-UniPathway
seryl-tRNA aminoacylation Source: UniProtKB

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Aminoacyl-tRNA synthetase, DNA-binding, Ligase
Biological process	Protein biosynthesis
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayⁱ	UPA00906; UER00895.

UniPathwayⁱ

UPA00906; UER00895.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Serine--tRNA ligase, cytoplasmic (EC:6.1.1.11By similarity) Alternative name(s): Seryl-tRNA synthetase Short name: SerRS Seryl-tRNA(Ser/Sec) synthetase
Gene namesⁱ	Name:Sars Synonyms:Sars1
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Chromosome 2

Organism-specific databases

Rat genome database More...RGDⁱ	628813. Sars.

RGDⁱ

628813. Sars.

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000270765	1 – 512	Serine--tRNA ligase, cytoplasmicAdd BLAST		512

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	1	N-acetylmethionineBy similarity	1
Modified residueⁱ	241	PhosphoserineBy similarity	1
Modified residueⁱ	323	N6-acetyllysineBy similarity	1

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

PaxDbⁱ	Q6P799.
PRIDEⁱ	Q6P799.

PTM databases

iPTMnetⁱ	Q6P799.
PhosphoSitePlusⁱ	Q6P799.

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSRNOG00000020255.
ExpressionAtlasⁱ	Q6P799. baseline and differential.
Genevisibleⁱ	Q6P799. RN.

Interactionⁱ

Subunit structureⁱ

Homodimer. The tRNA molecule may bind across the dimer. Interacts with SIRT2.By similarity

GO - Molecular functionⁱ

protein homodimerization activity
Source: RGD
Inferred from sequence orthologyⁱ
- 26433229

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

IntActⁱ	Q6P799. 1 interactor.
STRINGⁱ	10116.ENSRNOP00000038448.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q6P799.
SMRⁱ	Q6P799.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	9 – 61	Interaction with tRNABy similarityAdd BLAST		53

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	482 – 494	Nuclear localization signalBy similarityAdd BLAST		13

Domainⁱ

Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.By similarity

Sequence similaritiesⁱ

Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.Curated

Phylogenomic databases

eggNOGⁱ	KOG2509. Eukaryota. COG0172. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00790000123098.
HOGENOMⁱ	HOG000035937.
HOVERGENⁱ	HBG023172.
InParanoidⁱ	Q6P799.
KEGG Orthology (KO) More...KOⁱ	K01875.
PhylomeDBⁱ	Q6P799.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00770. SerRS_core. 1 hit.
InterProⁱ	View protein in InterPro IPR002314. aa-tRNA-synt_IIb. IPR006195. aa-tRNA-synth_II. IPR002317. Ser-tRNA-ligase_type_1. IPR015866. Ser-tRNA-synth_1_N. IPR033729. SerRS_core. IPR010978. tRNA-bd_arm.
PANTHERⁱ	PTHR11778. PTHR11778. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02403. Seryl_tRNA_N. 1 hit. PF00587. tRNA-synt_2b. 1 hit.
PIRSFⁱ	PIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTSⁱ	PR00981. TRNASYNTHSER.
SUPFAMⁱ	SSF46589. SSF46589. 1 hit.
TIGRFAMsⁱ	TIGR00414. serS. 1 hit.
PROSITEⁱ	View protein in PROSITE PS50862. AA_TRNA_LIGASE_II. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q6P799-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MVLDLDLFRV DKGGDPALIR ETQEKRFKDP GLVDQLVKAD SEWRRCRFRA 
        60         70         80         90        100
DNLNKLKNLC SKTIGEKMKK KEPVGEDESI PEDVLNFDDL TADTLAALKV 
       110        120        130        140        150
SQIKKVRLLV DEAIQKCDGE RVKLEAERFE NLREIGNLLH PSVPISNDED 
       160        170        180        190        200
ADNKVERIWG DCTVRKKYSH VDLVVMVDGF EGEKGAVVAG SRGYFLKGVL 
       210        220        230        240        250
VFLEQALIQY ALRTLGSRGY TPIYTPFFMR KEVMQEVAQL SQFDEELYKV 
       260        270        280        290        300
IGKGSEKSDD SSYDEKYLIA TSEQPIAALH RDEWLRPEDL PIKYAGFSTC 
       310        320        330        340        350
FRQEVGSHGR DTRGIFRVHQ FEKIEQFVYS SPHDNKSWEM FDEMITTAEE 
       360        370        380        390        400
FYQSLGIPYH IVNIVSGSLN HAASKKLDLE AWFPGSGAFR ELVSCSNCTD 
       410        420        430        440        450
YQARRLRIRY GQTKKMMDKV EFVHMLNATM CATTRTICAI LENYQTEKGI 
       460        470        480        490        500
VVPEKLREFM PPGLQELIPF VKPAPIDQEP SKKQKKQHEG SKKKAKEVTL 
       510 
ENQLQNMEVT EA

Length:512

Mass (Da):58,588

Last modified:January 23, 2007 - v3

Checksum:ⁱ960A30015F1F4A37

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BC061765 mRNA. Translation: AAH61765.1. AY145052 mRNA. Translation: AAN52758.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_001007607.1. NM_001007606.1.
UniGeneⁱ	Rn.73067.

Genome annotation databases

Ensemblⁱ	ENSRNOT00000033015; ENSRNOP00000038448; ENSRNOG00000020255.
GeneIDⁱ	266975.
KEGGⁱ	rno:266975.
UCSC genome browser More...UCSCⁱ	RGD:628813. rat.

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q6P799	P49591 UPI00025213CF UPI0003AFF94B A0A1U7TLA9 A0A2K5F7P5 H9FY84 UPI000C29E4CE A0A2K6AQK2 H0X989 UPI0004D089D4 +50	Homo sapiens (Human) Tarsius syrichta (Philippine tarsier) Aotus nancymaae (Ma's night monkey) Macaca mulatta (Rhesus macaque) Piliocolobus tephrosceles (Ugandan red Colobus) Macaca nemestrina (Pig-tailed macaque) Otolemur garnettii (Small-eared galago) (Garnett's greater bushbaby) Galeopterus variegatus (Malayan flying lemur) (Cynocephalus variegatus) Cavia porcellus (Guinea pig) Microcebus murinus (Lesser mouse lemur) (Gray Mouse Lemur) And more	514	UniRef90_P49591	Cluster: Serine--tRNA ligase, cytoplasmic	61

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q6P799	P49591 UPI00025213CF UPI0003AFF94B A0A1U7TLA9 A0A2K5F7P5 H0X989 K7BPF4 Q53HA4 UPI00045D6608 A0A286XG63 +79	Homo sapiens (Human) Tarsius syrichta (Philippine tarsier) Aotus nancymaae (Ma's night monkey) Otolemur garnettii (Small-eared galago) (Garnett's greater bushbaby) Pan troglodytes (Chimpanzee) Orycteropus afer afer Cavia porcellus (Guinea pig) Nomascus leucogenys (Northern white-cheeked gibbon) (Hylobates leucogenys) Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) Oryctolagus cuniculus (Rabbit) And more	514	UniRef50_P49591	Cluster: Serine--tRNA ligase, cytoplasmic	90

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BC061765 mRNA. Translation: AAH61765.1. AY145052 mRNA. Translation: AAN52758.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_001007607.1. NM_001007606.1.
UniGeneⁱ	Rn.73067.

3D structure databases

ProteinModelPortalⁱ	Q6P799.
SMRⁱ	Q6P799.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	Q6P799. 1 interactor.
STRINGⁱ	10116.ENSRNOP00000038448.

PTM databases

iPTMnetⁱ	Q6P799.
PhosphoSitePlusⁱ	Q6P799.

Proteomic databases

PaxDbⁱ	Q6P799.
PRIDEⁱ	Q6P799.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSRNOT00000033015; ENSRNOP00000038448; ENSRNOG00000020255.
GeneIDⁱ	266975.
KEGGⁱ	rno:266975.
UCSC genome browser More...UCSCⁱ	RGD:628813. rat.

Organism-specific databases

CTDⁱ	6301.
Rat genome database More...RGDⁱ	628813. Sars.

Phylogenomic databases

eggNOGⁱ	KOG2509. Eukaryota. COG0172. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00790000123098.
HOGENOMⁱ	HOG000035937.
HOVERGENⁱ	HBG023172.
InParanoidⁱ	Q6P799.
KEGG Orthology (KO) More...KOⁱ	K01875.
PhylomeDBⁱ	Q6P799.

Enzyme and pathway databases

UniPathwayⁱ	UPA00906; UER00895.

UniPathwayⁱ

UPA00906; UER00895.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q6P799.

PROⁱ

PR:Q6P799.

Gene expression databases

Bgeeⁱ	ENSRNOG00000020255.
ExpressionAtlasⁱ	Q6P799. baseline and differential.
Genevisibleⁱ	Q6P799. RN.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00770. SerRS_core. 1 hit.
InterProⁱ	View protein in InterPro IPR002314. aa-tRNA-synt_IIb. IPR006195. aa-tRNA-synth_II. IPR002317. Ser-tRNA-ligase_type_1. IPR015866. Ser-tRNA-synth_1_N. IPR033729. SerRS_core. IPR010978. tRNA-bd_arm.
PANTHERⁱ	PTHR11778. PTHR11778. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02403. Seryl_tRNA_N. 1 hit. PF00587. tRNA-synt_2b. 1 hit.
PIRSFⁱ	PIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTSⁱ	PR00981. TRNASYNTHSER.
SUPFAMⁱ	SSF46589. SSF46589. 1 hit.
TIGRFAMsⁱ	TIGR00414. serS. 1 hit.
PROSITEⁱ	View protein in PROSITE PS50862. AA_TRNA_LIGASE_II. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SYSC_RAT
Accessionⁱ	Q6P799Primary (citable) accession number: Q6P799 Secondary accession number(s): Q8CIQ8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
	Last sequence update:	January 23, 2007
	Last modified:	March 28, 2018
	This is version 119 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q6P799 (SYSC_RAT)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Serine--tRNA ligase, cytoplasmic

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: selenocysteinyl-tRNA(Sec) biosynthesis

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Extracellular region or secreted

Mitochondrion

Nucleus

Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Your basket is currently empty. ⁱ

Functionⁱ

Pathwayⁱ: selenocysteinyl-tRNA(Sec) biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ