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Protein

Serine--tRNA ligase, cytoplasmic

Gene

Sars

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) (By similarity).By similarity

Catalytic activityi

ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser).
ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec).

Pathwayi: selenocysteinyl-tRNA(Sec) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-seryl-tRNA(Sec) from L-serine and tRNA(Sec).
Proteins known to be involved in this subpathway in this organism are:
  1. Serine--tRNA ligase, cytoplasmic (Sars)
This subpathway is part of the pathway selenocysteinyl-tRNA(Sec) biosynthesis, which is itself part of Aminoacyl-tRNA biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-seryl-tRNA(Sec) from L-serine and tRNA(Sec), the pathway selenocysteinyl-tRNA(Sec) biosynthesis and in Aminoacyl-tRNA biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei318ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei325SerineBy similarity1
Binding sitei429SerineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi302 – 304ATPBy similarity3
Nucleotide bindingi391 – 394ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00906; UER00895.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine--tRNA ligase, cytoplasmic (EC:6.1.1.11)
Alternative name(s):
Seryl-tRNA synthetase
Short name:
SerRS
Seryl-tRNA(Ser/Sec) synthetase
Gene namesi
Name:Sars
Synonyms:Sars1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi628813. Sars.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002707651 – 512Serine--tRNA ligase, cytoplasmicAdd BLAST512

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei241PhosphoserineBy similarity1
Modified residuei323N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ6P799.
PRIDEiQ6P799.

PTM databases

iPTMnetiQ6P799.
PhosphoSitePlusiQ6P799.

Expressioni

Gene expression databases

BgeeiENSRNOG00000020255.
ExpressionAtlasiQ6P799. baseline and differential.
GenevisibleiQ6P799. RN.

Interactioni

Subunit structurei

Homodimer. The tRNA molecule binds across the dimer (By similarity).By similarity

Protein-protein interaction databases

IntActiQ6P799. 1 interactor.
STRINGi10116.ENSRNOP00000038448.

Structurei

3D structure databases

ProteinModelPortaliQ6P799.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni271 – 273Serine bindingBy similarity3

Domaini

Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2509. Eukaryota.
COG0172. LUCA.
GeneTreeiENSGT00790000123098.
HOGENOMiHOG000035937.
HOVERGENiHBG023172.
InParanoidiQ6P799.
KOiK01875.
PhylomeDBiQ6P799.

Family and domain databases

CDDicd00770. SerRS_core. 1 hit.
Gene3Di1.10.287.40. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR002317. Ser-tRNA-ligase_type_1.
IPR015866. Ser-tRNA-synth_1_N.
IPR033729. SerRS_core.
IPR010978. tRNA-bd_arm.
[Graphical view]
PANTHERiPTHR11778. PTHR11778. 1 hit.
PfamiPF02403. Seryl_tRNA_N. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR00981. TRNASYNTHSER.
SUPFAMiSSF46589. SSF46589. 1 hit.
TIGRFAMsiTIGR00414. serS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6P799-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLDLDLFRV DKGGDPALIR ETQEKRFKDP GLVDQLVKAD SEWRRCRFRA
60 70 80 90 100
DNLNKLKNLC SKTIGEKMKK KEPVGEDESI PEDVLNFDDL TADTLAALKV
110 120 130 140 150
SQIKKVRLLV DEAIQKCDGE RVKLEAERFE NLREIGNLLH PSVPISNDED
160 170 180 190 200
ADNKVERIWG DCTVRKKYSH VDLVVMVDGF EGEKGAVVAG SRGYFLKGVL
210 220 230 240 250
VFLEQALIQY ALRTLGSRGY TPIYTPFFMR KEVMQEVAQL SQFDEELYKV
260 270 280 290 300
IGKGSEKSDD SSYDEKYLIA TSEQPIAALH RDEWLRPEDL PIKYAGFSTC
310 320 330 340 350
FRQEVGSHGR DTRGIFRVHQ FEKIEQFVYS SPHDNKSWEM FDEMITTAEE
360 370 380 390 400
FYQSLGIPYH IVNIVSGSLN HAASKKLDLE AWFPGSGAFR ELVSCSNCTD
410 420 430 440 450
YQARRLRIRY GQTKKMMDKV EFVHMLNATM CATTRTICAI LENYQTEKGI
460 470 480 490 500
VVPEKLREFM PPGLQELIPF VKPAPIDQEP SKKQKKQHEG SKKKAKEVTL
510
ENQLQNMEVT EA
Length:512
Mass (Da):58,588
Last modified:January 23, 2007 - v3
Checksum:i960A30015F1F4A37
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC061765 mRNA. Translation: AAH61765.1.
AY145052 mRNA. Translation: AAN52758.1.
RefSeqiNP_001007607.1. NM_001007606.1.
UniGeneiRn.73067.

Genome annotation databases

EnsembliENSRNOT00000033015; ENSRNOP00000038448; ENSRNOG00000020255.
GeneIDi266975.
KEGGirno:266975.
UCSCiRGD:628813. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC061765 mRNA. Translation: AAH61765.1.
AY145052 mRNA. Translation: AAN52758.1.
RefSeqiNP_001007607.1. NM_001007606.1.
UniGeneiRn.73067.

3D structure databases

ProteinModelPortaliQ6P799.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6P799. 1 interactor.
STRINGi10116.ENSRNOP00000038448.

PTM databases

iPTMnetiQ6P799.
PhosphoSitePlusiQ6P799.

Proteomic databases

PaxDbiQ6P799.
PRIDEiQ6P799.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000033015; ENSRNOP00000038448; ENSRNOG00000020255.
GeneIDi266975.
KEGGirno:266975.
UCSCiRGD:628813. rat.

Organism-specific databases

CTDi6301.
RGDi628813. Sars.

Phylogenomic databases

eggNOGiKOG2509. Eukaryota.
COG0172. LUCA.
GeneTreeiENSGT00790000123098.
HOGENOMiHOG000035937.
HOVERGENiHBG023172.
InParanoidiQ6P799.
KOiK01875.
PhylomeDBiQ6P799.

Enzyme and pathway databases

UniPathwayiUPA00906; UER00895.

Miscellaneous databases

PROiQ6P799.

Gene expression databases

BgeeiENSRNOG00000020255.
ExpressionAtlasiQ6P799. baseline and differential.
GenevisibleiQ6P799. RN.

Family and domain databases

CDDicd00770. SerRS_core. 1 hit.
Gene3Di1.10.287.40. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR002317. Ser-tRNA-ligase_type_1.
IPR015866. Ser-tRNA-synth_1_N.
IPR033729. SerRS_core.
IPR010978. tRNA-bd_arm.
[Graphical view]
PANTHERiPTHR11778. PTHR11778. 1 hit.
PfamiPF02403. Seryl_tRNA_N. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR00981. TRNASYNTHSER.
SUPFAMiSSF46589. SSF46589. 1 hit.
TIGRFAMsiTIGR00414. serS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYSC_RAT
AccessioniPrimary (citable) accession number: Q6P799
Secondary accession number(s): Q8CIQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.