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Protein

Cyclic AMP-dependent transcription factor ATF-5

Gene

Atf5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4 (By similarity) (PubMed:19531563, PubMed:21212266). Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate (By similarity) (PubMed:12805299). Participates in the pathways by which SHH promotes cerebellar granule neuron progenitor cells proliferation. Critical for survival of mature olfactory sensory neurons (OSN), directs expression of OSN-specific genes (By similarity). May be involved in osteogenic differentiation. Promotes cell proliferation and survival by inducing the expression of EGR1 sinergistically with ELK1. Once acetylated by EP300, binds to ARE sequences on target genes promoters, such as BCL2 and EGR1 (By similarity) (PubMed:21791614). Plays an anti-apoptotic role through the transcriptional regulation of BCL2, this function seems to be cell type-dependent (By similarity). Cooperates with NR1I3/CAR in the transcriptional activation of CYP2B6 in liver. In hepatic cells, represses CRE-dependent transcription and inhibits proliferation by blocking at G2/M phase. May act as a negative regulator of IL1B transduction pathway in liver. Upon IL1B stimulus, cooperates with NLK to activate the transactivation activity of C/EBP subfamily members. Besides its function of transcription factor, acts as a cofactor of CEBPB to activate CEBPA and promote adipocyte differentiation. Regulates centrosome dynamics in a cell-cycle- and centriole-age-dependent manner. Forms 9-foci symmetrical ring scaffold around the mother centriole to control centrosome function and the interaction between centrioles and pericentriolar material (By similarity).By similarity4 Publications

GO - Molecular functioni

  • chromatin binding Source: UniProtKB
  • heat shock protein binding Source: RGD
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: GO_Central
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: GO_Central
  • sequence-specific DNA binding Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: UniProtKB
  • transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic AMP-dependent transcription factor ATF-5
Short name:
cAMP-dependent transcription factor ATF-5
Alternative name(s):
Activating transcription factor 5
Transcription factor ATFx
Gene namesi
Name:Atf5
Synonyms:Atfx
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628902. Atf5.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus PROSITE-ProRule annotationBy similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity

  • Note: Actively transported to the centrosome and accumulated in the pericentriolar material (PCM) during G1 to M phase via a microtubule-dependent mechanism. During late telophase and cytokinesis, translocates from the centrosome to the midbody.By similarity

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi29K → R: Not acetylated by EP300. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000765881 – 281Cyclic AMP-dependent transcription factor ATF-5Add BLAST281

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei29N6-acetyllysine; by EP3001 Publication1
Modified residuei255PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated by CDC34 and UBE2B in order to be degraded by the proteasome.By similarity
Acetylated at Lys-29 by EP300, the acetylation enhances the interaction with CEBPB, DNA-binding and transactivation activity.1 Publication
Ubiquitinated by CDC34 and UBE2B in order to be degraded by the proteasome. Cisplatin inhibits ubiquitination and proteasome-mediated degradation by inhibiting the interaction with CDC34. Ubiquitination and degradation by the proteasome are inhibited by NLK in a kinase-independent manner.By similarity
Phosphorylated by NLK, probably at Ser-92 and Ser-126.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6P788.

PTM databases

PhosphoSitePlusiQ6P788.

Expressioni

Developmental stagei

Expressed in embryonic nasal epithelium, dorsal root, trigeminal ganglia, brain and liver. Within the brain, expression is highest in the ventricular zone and decreased in structures containing migrating and postmitotic neurons (at protein level).1 Publication

Inductioni

Down-regulated during neuronal differentiation. Down-regulated by pro-apoptotic stimuli (PubMed:21212266).2 Publications

Gene expression databases

BgeeiENSRNOG00000020060.

Interactioni

Subunit structurei

Binds DNA as a dimer. Interacts with PTP4A1/PRL-1 (By similarity). Interacts with CCND3, but not with CCND1 or CCND2. Interacts with HSPA1A or HSPA1B; the interaction protects ATF5 from degradation via proteasome-dependent and caspase-dependent processes. Interacts (via C-terminal region) with NPM1 (via C-terminal region); the interaction leads to loss of association between HSPA1A or HSPA1B and ATF5 and promotes ATF5 degradation via proteasome-dependent and caspase-dependent processes. Interacts with NLK; the interaction stabilizes ATF5 at the protein level in a kinase-independent manner. Interacts with alpha-tubulin, gamma-tubulin members TUBGCP2 and TUBGCP4, PCNT; the ATF5:PCNT:polyglutamylated tubulin (PGT) tripartite unites the mother centriole and the pericentriolar material (PCM) in the centrosome (By similarity). Interacts with CEBPB and EP300; EP300 is required for ATF5 and CEBPB interaction and DNA binding (By similarity) (PubMed:21791614).By similarity1 Publication

GO - Molecular functioni

  • heat shock protein binding Source: RGD

Protein-protein interaction databases

BioGridi251873. 1 interactor.
STRINGi10116.ENSRNOP00000027260.

Structurei

3D structure databases

ProteinModelPortaliQ6P788.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini207 – 270bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 21Required for protein stabilization induced by IL1BBy similarityAdd BLAST21
Regioni119 – 216Interaction with PTP4A1By similarityAdd BLAST98
Regioni209 – 229Basic motifPROSITE-ProRule annotationAdd BLAST21
Regioni235 – 249Leucine-zipperPROSITE-ProRule annotationAdd BLAST15

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi123 – 135Poly-ProAdd BLAST13

Sequence similaritiesi

Belongs to the bZIP family.Curated
Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4571. Eukaryota.
ENOG4111ZXT. LUCA.
HOGENOMiHOG000004844.
HOVERGENiHBG003535.
InParanoidiQ6P788.
KOiK09044.
PhylomeDBiQ6P788.
TreeFamiTF316136.

Family and domain databases

InterProiIPR029855. ATF-5.
IPR004827. bZIP.
[Graphical view]
PANTHERiPTHR13044:SF3. PTHR13044:SF3. 1 hit.
PfamiPF00170. bZIP_1. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6P788-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLLATLGLE LDRALLPASG LGWLVDYGKL PLAPAPLGPY EVLGGALEGG
60 70 80 90 100
LPGGGEPLAG DGFSDWMTER VDFTALLPLE APLPPGTLPP PSPAPPDLEA
110 120 130 140 150
MASLLKKELE QMEDFFLDAP LLPPPSPPPP PPPAPSLPLP LPLPTFDLPQ
160 170 180 190 200
PPTLDTLDLL AVYCRSEAGP GDSGLTTLPV PQQPPPLAPL PSLSRPAPYP
210 220 230 240 250
SPASTRGDRK QKKRDQNKSA ALRYRQRKRA EGEALEGECQ GLEARNRELR
260 270 280
ERAESVEREI QYVKDLLIEV YKARSQRTRS A
Length:281
Mass (Da):30,205
Last modified:July 5, 2004 - v1
Checksum:iFBBF68FB44F9C601
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti193L → P in AAM92263 (PubMed:12805299).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY123225 mRNA. Translation: AAM92263.1.
BC061786 mRNA. Translation: AAH61786.1.
RefSeqiNP_758839.3. NM_172336.3.
XP_006229042.1. XM_006228980.2.
XP_017444334.1. XM_017588845.1.
UniGeneiRn.229186.

Genome annotation databases

GeneIDi282840.
KEGGirno:282840.
UCSCiRGD:628902. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY123225 mRNA. Translation: AAM92263.1.
BC061786 mRNA. Translation: AAH61786.1.
RefSeqiNP_758839.3. NM_172336.3.
XP_006229042.1. XM_006228980.2.
XP_017444334.1. XM_017588845.1.
UniGeneiRn.229186.

3D structure databases

ProteinModelPortaliQ6P788.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251873. 1 interactor.
STRINGi10116.ENSRNOP00000027260.

PTM databases

PhosphoSitePlusiQ6P788.

Proteomic databases

PaxDbiQ6P788.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi282840.
KEGGirno:282840.
UCSCiRGD:628902. rat.

Organism-specific databases

CTDi22809.
RGDi628902. Atf5.

Phylogenomic databases

eggNOGiKOG4571. Eukaryota.
ENOG4111ZXT. LUCA.
HOGENOMiHOG000004844.
HOVERGENiHBG003535.
InParanoidiQ6P788.
KOiK09044.
PhylomeDBiQ6P788.
TreeFamiTF316136.

Miscellaneous databases

PROiQ6P788.

Gene expression databases

BgeeiENSRNOG00000020060.

Family and domain databases

InterProiIPR029855. ATF-5.
IPR004827. bZIP.
[Graphical view]
PANTHERiPTHR13044:SF3. PTHR13044:SF3. 1 hit.
PfamiPF00170. bZIP_1. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATF5_RAT
AccessioniPrimary (citable) accession number: Q6P788
Secondary accession number(s): Q8CIT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.