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Q6P768

- B4GT3_RAT

UniProt

Q6P768 - B4GT3_RAT

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Protein

Beta-1,4-galactosyltransferase 3

Gene

B4galt3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.By similarity

Catalytic activityi

UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.
UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

Cofactori

Mn2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi199 – 1991ManganeseBy similarity
Binding sitei228 – 2281UDP-alpha-D-galactoseBy similarity
Binding sitei260 – 2601UDP-alpha-D-galactoseBy similarity
Metal bindingi293 – 2931Manganese; via tele nitrogenBy similarity
Binding sitei305 – 3051N-acetyl-D-glucosamineBy similarity

GO - Molecular functioni

  1. beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. N-acetyllactosamine synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_196475. Keratan sulfate biosynthesis.
REACT_199227. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 3 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 3
Short name:
Beta4Gal-T3
Short name:
b4Gal-T3
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3
Including the following 3 domains:
N-acetyllactosamine synthase (EC:2.4.1.90)
Alternative name(s):
Nal synthase
Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC:2.4.1.38)
Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC:2.4.1.-)
Gene namesi
Name:B4galt3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 13

Organism-specific databases

RGDi1359221. B4galt3.

Subcellular locationi

Golgi apparatusGolgi stack membrane By similarity; Single-pass type II membrane protein By similarity
Note: Trans cisternae of Golgi stack.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1010CytoplasmicSequence Analysis
Transmembranei11 – 3121Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini32 – 395364LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. Golgi apparatus Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 395395Beta-1,4-galactosyltransferase 3PRO_0000080540Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi57 – 571N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi79 ↔ 121By similarity
Glycosylationi168 – 1681N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi192 ↔ 211By similarity
Glycosylationi339 – 3391N-linked (GlcNAc...)Sequence Analysis
Glycosylationi387 – 3871N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

PTM databases

PhosphoSiteiQ6P768.

Expressioni

Gene expression databases

GenevestigatoriQ6P768.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004735.

Structurei

3D structure databases

ProteinModelPortaliQ6P768.
SMRiQ6P768. Positions 76-346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni132 – 1365UDP-alpha-D-galactose bindingBy similarity
Regioni171 – 1733UDP-alpha-D-galactose bindingBy similarity
Regioni198 – 1992UDP-alpha-D-galactose bindingBy similarity
Regioni262 – 2654N-acetyl-D-glucosamine bindingBy similarity
Regioni293 – 2953UDP-alpha-D-galactose bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG327897.
GeneTreeiENSGT00760000119140.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiQ6P768.
KOiK07968.
OMAiALFGREQ.
OrthoDBiEOG7060R0.
PhylomeDBiQ6P768.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6P768-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLRRLLERPC TLALLVGSQL AVMMYLSLGG FRSLSALFGR DPGPTFDYSH
60 70 80 90 100
PHDVYSNLSH LPGAPGAAGA PLAQVLPDCP ERSPFLVGPV SVSFSPVPSL
110 120 130 140 150
AEIVERNPRV ESGGRYRPAG CEPRSRTAII VPHRAREHHL RLLLYHLHPF
160 170 180 190 200
LQRQQLAYGI YVIHQAGNGT FNRAKLLNVG VREALRDEEW DCLFLHDVDL
210 220 230 240 250
LPENDHNLYV CDPRGPRHVA VAMNKFGYSL PYPQYFGGVS ALTPDQYLKM
260 270 280 290 300
NGFPNEYWGW GGEDDDIATR VRLAGMKISR PPTSVGHYKM VKHRGDKGNE
310 320 330 340 350
ENPHRFDLLV RTQNSWTQDG MNSLTYRLLA RELGPLYTNI TADIGTDPRG
360 370 380 390
PRAPSGPRYP PGSSQAFRQE MLQRRPPARP GPLPTANHTA PHGSH
Length:395
Mass (Da):44,031
Last modified:July 5, 2004 - v1
Checksum:i72BF0E9C7366ED2C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC061812 mRNA. Translation: AAH61812.1.
RefSeqiNP_001009539.1. NM_001009539.1.
XP_006250315.1. XM_006250253.2.
XP_006250316.1. XM_006250254.2.
UniGeneiRn.91751.

Genome annotation databases

EnsembliENSRNOT00000004735; ENSRNOP00000004735; ENSRNOG00000003551.
GeneIDi494342.
KEGGirno:494342.
UCSCiRGD:1359221. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC061812 mRNA. Translation: AAH61812.1 .
RefSeqi NP_001009539.1. NM_001009539.1.
XP_006250315.1. XM_006250253.2.
XP_006250316.1. XM_006250254.2.
UniGenei Rn.91751.

3D structure databases

ProteinModelPortali Q6P768.
SMRi Q6P768. Positions 76-346.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000004735.

Protein family/group databases

CAZyi GT7. Glycosyltransferase Family 7.

PTM databases

PhosphoSitei Q6P768.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000004735 ; ENSRNOP00000004735 ; ENSRNOG00000003551 .
GeneIDi 494342.
KEGGi rno:494342.
UCSCi RGD:1359221. rat.

Organism-specific databases

CTDi 8703.
RGDi 1359221. B4galt3.

Phylogenomic databases

eggNOGi NOG327897.
GeneTreei ENSGT00760000119140.
HOGENOMi HOG000231027.
HOVERGENi HBG058334.
InParanoidi Q6P768.
KOi K07968.
OMAi ALFGREQ.
OrthoDBi EOG7060R0.
PhylomeDBi Q6P768.
TreeFami TF312834.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_196475. Keratan sulfate biosynthesis.
REACT_199227. N-Glycan antennae elongation.

Miscellaneous databases

NextBioi 697612.
PROi Q6P768.

Gene expression databases

Genevestigatori Q6P768.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR19300. PTHR19300. 1 hit.
Pfami PF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view ]
PRINTSi PR02050. B14GALTRFASE.
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.

Entry informationi

Entry nameiB4GT3_RAT
AccessioniPrimary (citable) accession number: Q6P768
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: July 5, 2004
Last modified: November 26, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3