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Q6P768

- B4GT3_RAT

UniProt

Q6P768 - B4GT3_RAT

Protein

Beta-1,4-galactosyltransferase 3

Gene

B4galt3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.By similarity

    Catalytic activityi

    UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.
    UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

    Cofactori

    Manganese.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi199 – 1991ManganeseBy similarity
    Binding sitei228 – 2281UDP-alpha-D-galactoseBy similarity
    Binding sitei260 – 2601UDP-alpha-D-galactoseBy similarity
    Metal bindingi293 – 2931Manganese; via tele nitrogenBy similarity
    Binding sitei305 – 3051N-acetyl-D-glucosamineBy similarity

    GO - Molecular functioni

    1. beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW
    3. N-acetyllactosamine synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_196475. Keratan sulfate biosynthesis.
    REACT_199227. N-Glycan antennae elongation.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT7. Glycosyltransferase Family 7.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,4-galactosyltransferase 3 (EC:2.4.1.-)
    Short name:
    Beta-1,4-GalTase 3
    Short name:
    Beta4Gal-T3
    Short name:
    b4Gal-T3
    Alternative name(s):
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3
    Including the following 3 domains:
    N-acetyllactosamine synthase (EC:2.4.1.90)
    Alternative name(s):
    Nal synthase
    Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC:2.4.1.38)
    Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC:2.4.1.-)
    Gene namesi
    Name:B4galt3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 13

    Organism-specific databases

    RGDi1359221. B4galt3.

    Subcellular locationi

    Golgi apparatusGolgi stack membrane By similarity; Single-pass type II membrane protein By similarity
    Note: Trans cisternae of Golgi stack.By similarity

    GO - Cellular componenti

    1. Golgi cisterna membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 395395Beta-1,4-galactosyltransferase 3PRO_0000080540Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi57 – 571N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi79 ↔ 121By similarity
    Glycosylationi168 – 1681N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi192 ↔ 211By similarity
    Glycosylationi339 – 3391N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi387 – 3871N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    PTM databases

    PhosphoSiteiQ6P768.

    Expressioni

    Gene expression databases

    GenevestigatoriQ6P768.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000004735.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6P768.
    SMRiQ6P768. Positions 76-346.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1010CytoplasmicSequence Analysis
    Topological domaini32 – 395364LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei11 – 3121Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni132 – 1365UDP-alpha-D-galactose bindingBy similarity
    Regioni171 – 1733UDP-alpha-D-galactose bindingBy similarity
    Regioni198 – 1992UDP-alpha-D-galactose bindingBy similarity
    Regioni262 – 2654N-acetyl-D-glucosamine bindingBy similarity
    Regioni293 – 2953UDP-alpha-D-galactose bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 7 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG327897.
    GeneTreeiENSGT00750000117412.
    HOGENOMiHOG000231027.
    HOVERGENiHBG058334.
    InParanoidiQ6P768.
    KOiK07968.
    OMAiALFGREQ.
    OrthoDBiEOG7060R0.
    PhylomeDBiQ6P768.
    TreeFamiTF312834.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR19300. PTHR19300. 1 hit.
    PfamiPF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view]
    PRINTSiPR02050. B14GALTRFASE.
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q6P768-1 [UniParc]FASTAAdd to Basket

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    MLRRLLERPC TLALLVGSQL AVMMYLSLGG FRSLSALFGR DPGPTFDYSH    50
    PHDVYSNLSH LPGAPGAAGA PLAQVLPDCP ERSPFLVGPV SVSFSPVPSL 100
    AEIVERNPRV ESGGRYRPAG CEPRSRTAII VPHRAREHHL RLLLYHLHPF 150
    LQRQQLAYGI YVIHQAGNGT FNRAKLLNVG VREALRDEEW DCLFLHDVDL 200
    LPENDHNLYV CDPRGPRHVA VAMNKFGYSL PYPQYFGGVS ALTPDQYLKM 250
    NGFPNEYWGW GGEDDDIATR VRLAGMKISR PPTSVGHYKM VKHRGDKGNE 300
    ENPHRFDLLV RTQNSWTQDG MNSLTYRLLA RELGPLYTNI TADIGTDPRG 350
    PRAPSGPRYP PGSSQAFRQE MLQRRPPARP GPLPTANHTA PHGSH 395
    Length:395
    Mass (Da):44,031
    Last modified:July 5, 2004 - v1
    Checksum:i72BF0E9C7366ED2C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC061812 mRNA. Translation: AAH61812.1.
    RefSeqiNP_001009539.1. NM_001009539.1.
    XP_006250315.1. XM_006250253.1.
    XP_006250316.1. XM_006250254.1.
    UniGeneiRn.91751.

    Genome annotation databases

    EnsembliENSRNOT00000004735; ENSRNOP00000004735; ENSRNOG00000003551.
    GeneIDi494342.
    KEGGirno:494342.
    UCSCiRGD:1359221. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC061812 mRNA. Translation: AAH61812.1 .
    RefSeqi NP_001009539.1. NM_001009539.1.
    XP_006250315.1. XM_006250253.1.
    XP_006250316.1. XM_006250254.1.
    UniGenei Rn.91751.

    3D structure databases

    ProteinModelPortali Q6P768.
    SMRi Q6P768. Positions 76-346.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000004735.

    Protein family/group databases

    CAZyi GT7. Glycosyltransferase Family 7.

    PTM databases

    PhosphoSitei Q6P768.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000004735 ; ENSRNOP00000004735 ; ENSRNOG00000003551 .
    GeneIDi 494342.
    KEGGi rno:494342.
    UCSCi RGD:1359221. rat.

    Organism-specific databases

    CTDi 8703.
    RGDi 1359221. B4galt3.

    Phylogenomic databases

    eggNOGi NOG327897.
    GeneTreei ENSGT00750000117412.
    HOGENOMi HOG000231027.
    HOVERGENi HBG058334.
    InParanoidi Q6P768.
    KOi K07968.
    OMAi ALFGREQ.
    OrthoDBi EOG7060R0.
    PhylomeDBi Q6P768.
    TreeFami TF312834.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_196475. Keratan sulfate biosynthesis.
    REACT_199227. N-Glycan antennae elongation.

    Miscellaneous databases

    NextBioi 697612.
    PROi Q6P768.

    Gene expression databases

    Genevestigatori Q6P768.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR19300. PTHR19300. 1 hit.
    Pfami PF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view ]
    PRINTSi PR02050. B14GALTRFASE.
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Prostate.

    Entry informationi

    Entry nameiB4GT3_RAT
    AccessioniPrimary (citable) accession number: Q6P768
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 21, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3