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Q6P768

- B4GT3_RAT

UniProt

Q6P768 - B4GT3_RAT

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Protein
Beta-1,4-galactosyltransferase 3
Gene
B4galt3
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids By similarity.

Catalytic activityi

UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.
UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

Cofactori

Manganese By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi199 – 1991Manganese By similarity
Binding sitei228 – 2281UDP-alpha-D-galactose By similarity
Binding sitei260 – 2601UDP-alpha-D-galactose By similarity
Metal bindingi293 – 2931Manganese; via tele nitrogen By similarity
Binding sitei305 – 3051N-acetyl-D-glucosamine By similarity

GO - Molecular functioni

  1. N-acetyllactosamine synthase activity Source: UniProtKB-EC
  2. beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_196475. Keratan sulfate biosynthesis.
REACT_199227. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 3 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 3
Short name:
Beta4Gal-T3
Short name:
b4Gal-T3
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3
Including the following 3 domains:
N-acetyllactosamine synthase (EC:2.4.1.90)
Alternative name(s):
Nal synthase
Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC:2.4.1.38)
Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC:2.4.1.-)
Gene namesi
Name:B4galt3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 13

Organism-specific databases

RGDi1359221. B4galt3.

Subcellular locationi

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein By similarity
Note: Trans cisternae of Golgi stack By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1010Cytoplasmic Reviewed prediction
Transmembranei11 – 3121Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini32 – 395364Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi cisterna membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 395395Beta-1,4-galactosyltransferase 3
PRO_0000080540Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi57 – 571N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi79 ↔ 121 By similarity
Glycosylationi168 – 1681N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi192 ↔ 211 By similarity
Glycosylationi339 – 3391N-linked (GlcNAc...) Reviewed prediction
Glycosylationi387 – 3871N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

PTM databases

PhosphoSiteiQ6P768.

Expressioni

Gene expression databases

GenevestigatoriQ6P768.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004735.

Structurei

3D structure databases

ProteinModelPortaliQ6P768.
SMRiQ6P768. Positions 76-346.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni132 – 1365UDP-alpha-D-galactose binding By similarity
Regioni171 – 1733UDP-alpha-D-galactose binding By similarity
Regioni198 – 1992UDP-alpha-D-galactose binding By similarity
Regioni262 – 2654N-acetyl-D-glucosamine binding By similarity
Regioni293 – 2953UDP-alpha-D-galactose binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG327897.
GeneTreeiENSGT00750000117412.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiQ6P768.
KOiK07968.
OMAiALFGREQ.
OrthoDBiEOG7060R0.
PhylomeDBiQ6P768.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6P768-1 [UniParc]FASTAAdd to Basket

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MLRRLLERPC TLALLVGSQL AVMMYLSLGG FRSLSALFGR DPGPTFDYSH    50
PHDVYSNLSH LPGAPGAAGA PLAQVLPDCP ERSPFLVGPV SVSFSPVPSL 100
AEIVERNPRV ESGGRYRPAG CEPRSRTAII VPHRAREHHL RLLLYHLHPF 150
LQRQQLAYGI YVIHQAGNGT FNRAKLLNVG VREALRDEEW DCLFLHDVDL 200
LPENDHNLYV CDPRGPRHVA VAMNKFGYSL PYPQYFGGVS ALTPDQYLKM 250
NGFPNEYWGW GGEDDDIATR VRLAGMKISR PPTSVGHYKM VKHRGDKGNE 300
ENPHRFDLLV RTQNSWTQDG MNSLTYRLLA RELGPLYTNI TADIGTDPRG 350
PRAPSGPRYP PGSSQAFRQE MLQRRPPARP GPLPTANHTA PHGSH 395
Length:395
Mass (Da):44,031
Last modified:July 5, 2004 - v1
Checksum:i72BF0E9C7366ED2C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC061812 mRNA. Translation: AAH61812.1.
RefSeqiNP_001009539.1. NM_001009539.1.
XP_006250315.1. XM_006250253.1.
XP_006250316.1. XM_006250254.1.
UniGeneiRn.91751.

Genome annotation databases

EnsembliENSRNOT00000004735; ENSRNOP00000004735; ENSRNOG00000003551.
GeneIDi494342.
KEGGirno:494342.
UCSCiRGD:1359221. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC061812 mRNA. Translation: AAH61812.1 .
RefSeqi NP_001009539.1. NM_001009539.1.
XP_006250315.1. XM_006250253.1.
XP_006250316.1. XM_006250254.1.
UniGenei Rn.91751.

3D structure databases

ProteinModelPortali Q6P768.
SMRi Q6P768. Positions 76-346.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000004735.

Protein family/group databases

CAZyi GT7. Glycosyltransferase Family 7.

PTM databases

PhosphoSitei Q6P768.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000004735 ; ENSRNOP00000004735 ; ENSRNOG00000003551 .
GeneIDi 494342.
KEGGi rno:494342.
UCSCi RGD:1359221. rat.

Organism-specific databases

CTDi 8703.
RGDi 1359221. B4galt3.

Phylogenomic databases

eggNOGi NOG327897.
GeneTreei ENSGT00750000117412.
HOGENOMi HOG000231027.
HOVERGENi HBG058334.
InParanoidi Q6P768.
KOi K07968.
OMAi ALFGREQ.
OrthoDBi EOG7060R0.
PhylomeDBi Q6P768.
TreeFami TF312834.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_196475. Keratan sulfate biosynthesis.
REACT_199227. N-Glycan antennae elongation.

Miscellaneous databases

NextBioi 697612.
PROi Q6P768.

Gene expression databases

Genevestigatori Q6P768.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR19300. PTHR19300. 1 hit.
Pfami PF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view ]
PRINTSi PR02050. B14GALTRFASE.
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.

Entry informationi

Entry nameiB4GT3_RAT
AccessioniPrimary (citable) accession number: Q6P768
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: July 5, 2004
Last modified: September 3, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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