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Q6P768 (B4GT3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-1,4-galactosyltransferase 3

Short name=Beta-1,4-GalTase 3
Short name=Beta4Gal-T3
Short name=b4Gal-T3
EC=2.4.1.-
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3

Including the following 3 domains:

  1. N-acetyllactosamine synthase
    EC=2.4.1.90
    Alternative name(s):
    Nal synthase
  2. Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase
    EC=2.4.1.38
  3. Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
    EC=2.4.1.-
Gene names
Name:B4galt3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length395 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids By similarity.

Catalytic activity

UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.

UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

Cofactor

Manganese By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein By similarity. Note: Trans cisternae of Golgi stack By similarity.

Sequence similarities

Belongs to the glycosyltransferase 7 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 395395Beta-1,4-galactosyltransferase 3
PRO_0000080540

Regions

Topological domain1 – 1010Cytoplasmic Potential
Transmembrane11 – 3121Helical; Signal-anchor for type II membrane protein; Potential
Topological domain32 – 395364Lumenal Potential
Region132 – 1365UDP-alpha-D-galactose binding By similarity
Region171 – 1733UDP-alpha-D-galactose binding By similarity
Region198 – 1992UDP-alpha-D-galactose binding By similarity
Region262 – 2654N-acetyl-D-glucosamine binding By similarity
Region293 – 2953UDP-alpha-D-galactose binding By similarity

Sites

Metal binding1991Manganese By similarity
Metal binding2931Manganese; via tele nitrogen By similarity
Binding site2281UDP-alpha-D-galactose By similarity
Binding site2601UDP-alpha-D-galactose By similarity
Binding site3051N-acetyl-D-glucosamine By similarity

Amino acid modifications

Glycosylation571N-linked (GlcNAc...) Potential
Glycosylation1681N-linked (GlcNAc...) Potential
Glycosylation3391N-linked (GlcNAc...) Potential
Glycosylation3871N-linked (GlcNAc...) Potential
Disulfide bond79 ↔ 121 By similarity
Disulfide bond192 ↔ 211 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6P768 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 72BF0E9C7366ED2C

FASTA39544,031
        10         20         30         40         50         60 
MLRRLLERPC TLALLVGSQL AVMMYLSLGG FRSLSALFGR DPGPTFDYSH PHDVYSNLSH 

        70         80         90        100        110        120 
LPGAPGAAGA PLAQVLPDCP ERSPFLVGPV SVSFSPVPSL AEIVERNPRV ESGGRYRPAG 

       130        140        150        160        170        180 
CEPRSRTAII VPHRAREHHL RLLLYHLHPF LQRQQLAYGI YVIHQAGNGT FNRAKLLNVG 

       190        200        210        220        230        240 
VREALRDEEW DCLFLHDVDL LPENDHNLYV CDPRGPRHVA VAMNKFGYSL PYPQYFGGVS 

       250        260        270        280        290        300 
ALTPDQYLKM NGFPNEYWGW GGEDDDIATR VRLAGMKISR PPTSVGHYKM VKHRGDKGNE 

       310        320        330        340        350        360 
ENPHRFDLLV RTQNSWTQDG MNSLTYRLLA RELGPLYTNI TADIGTDPRG PRAPSGPRYP 

       370        380        390 
PGSSQAFRQE MLQRRPPARP GPLPTANHTA PHGSH 

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC061812 mRNA. Translation: AAH61812.1.
RefSeqNP_001009539.1. NM_001009539.1.
XP_006250315.1. XM_006250253.1.
XP_006250316.1. XM_006250254.1.
UniGeneRn.91751.

3D structure databases

ProteinModelPortalQ6P768.
SMRQ6P768. Positions 76-346.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000004735.

Protein family/group databases

CAZyGT7. Glycosyltransferase Family 7.

PTM databases

PhosphoSiteQ6P768.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000004735; ENSRNOP00000004735; ENSRNOG00000003551.
GeneID494342.
KEGGrno:494342.
UCSCRGD:1359221. rat.

Organism-specific databases

CTD8703.
RGD1359221. B4galt3.

Phylogenomic databases

eggNOGNOG327897.
GeneTreeENSGT00750000117412.
HOGENOMHOG000231027.
HOVERGENHBG058334.
InParanoidQ6P768.
KOK07968.
OMAALFGREQ.
OrthoDBEOG7060R0.
PhylomeDBQ6P768.
TreeFamTF312834.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

GenevestigatorQ6P768.

Family and domain databases

Gene3D3.90.550.10. 1 hit.
InterProIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERPTHR19300. PTHR19300. 1 hit.
PfamPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSPR02050. B14GALTRFASE.
SUPFAMSSF53448. SSF53448. 1 hit.
ProtoNetSearch...

Other

NextBio697612.
PROQ6P768.

Entry information

Entry nameB4GT3_RAT
AccessionPrimary (citable) accession number: Q6P768
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways