Reviewed, UniProtKB/Swiss-Prot Q6P768 (B4GT3_RAT)

Names and origin

Protein names

Recommended name:
    Beta-1,4-galactosyltransferase 3
      Short name=Beta-1,4-GalTase 3
      Short name=Beta4Gal-T3
      Short name=b4Gal-T3
    EC=2.4.1.-
Alternative name(s):
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3
Including the following 3 domains:
    1- Recommended name:
            N-acetyllactosamine synthase
              EC=2.4.1.90
        Alternative name(s):
            Nal synthetase
    2- Recommended name:
            Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase
              EC=2.4.1.38
    3- Recommended name:
            Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
              EC=2.4.1.-

Gene names

Name:

B4galt3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids By similarity.
Catalytic activity	UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide. UDP-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Trans cisternae of Golgi stack By similarity.
Sequence similarities	Belongs to the glycosyltransferase 7 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetyllactosamine synthase activity Inferred from electronic annotation. Source: EC beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Beta-1,4-galactosyltransferase 3		PRO_0000080540
Regions
	Topological domain	1 – 10	10	Cytoplasmic Potential
	Transmembrane	11 – 31	21	Signal-anchor for type II membrane protein Potential
	Topological domain	32 – 395	364	Lumenal Potential
Sites
	Metal binding	199	1	Manganese By similarity
	Metal binding	293	1	Manganese By similarity
Amino acid modifications
	Glycosylation	57	1	N-linked (GlcNAc...) Potential
	Glycosylation	168	1	N-linked (GlcNAc...) Potential
	Glycosylation	339	1	N-linked (GlcNAc...) Potential
	Glycosylation	387	1	N-linked (GlcNAc...) Potential
	Disulfide bond	79 ↔ 121		By similarity
	Disulfide bond	192 ↔ 211		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6P768-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 72BF0E9C7366ED2C	FASTA	395	44,031
	10 20 30 40 50 60 MLRRLLERPC TLALLVGSQL AVMMYLSLGG FRSLSALFGR DPGPTFDYSH PHDVYSNLSH 70 80 90 100 110 120 LPGAPGAAGA PLAQVLPDCP ERSPFLVGPV SVSFSPVPSL AEIVERNPRV ESGGRYRPAG 130 140 150 160 170 180 CEPRSRTAII VPHRAREHHL RLLLYHLHPF LQRQQLAYGI YVIHQAGNGT FNRAKLLNVG 190 200 210 220 230 240 VREALRDEEW DCLFLHDVDL LPENDHNLYV CDPRGPRHVA VAMNKFGYSL PYPQYFGGVS 250 260 270 280 290 300 ALTPDQYLKM NGFPNEYWGW GGEDDDIATR VRLAGMKISR PPTSVGHYKM VKHRGDKGNE 310 320 330 340 350 360 ENPHRFDLLV RTQNSWTQDG MNSLTYRLLA RELGPLYTNI TADIGTDPRG PRAPSGPRYP 370 380 390 PGSSQAFRQE MLQRRPPARP GPLPTANHTA PHGSH

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC061812 mRNA. Translation: AAH61812.1.
IPI	IPI00421551.
RefSeq	NP_001009539.1.
UniGene	Rn.91751
3D structure databases
SMR	Q6P768. Positions 76-346.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6P768.
Protein family/group databases
CAZy	GT7. Glycosyltransferase Family 7.
Genome annotation databases
Ensembl	ENSRNOT00000004735; ENSRNOP00000004735; ENSRNOG00000003551; Rattus norvegicus. [Genome view]
GeneID	494342.
KEGG	rno:494342.
Organism-specific databases
CTD	494342.
RGD	1359221. B4galt3.
Phylogenomic databases
eggNOG	roNOG07738.
HOVERGEN	Q6P768.
InParanoid	Q6P768.
Enzyme and pathway databases
BRENDA	2.4.1.38. 248. 2.4.1.90. 248.
Gene expression databases
ArrayExpress	Q6P768.
Genevestigator	Q6P768.
GermOnline	ENSRNOG00000003551. Rattus norvegicus.
Family and domain databases
InterPro	IPR003859. Galactosyl_T_2. [Graphical view]
PANTHER	PTHR19300. Galactosyl_T_2. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	697612.

Entry information

Entry name

B4GT3_RAT

Accession

Primary (citable) accession number: Q6P768

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)