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Protein

Adaptin ear-binding coat-associated protein 2

Gene

Necap2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in endocytosis.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Endocytosis, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Adaptin ear-binding coat-associated protein 2
Short name:
NECAP-2
Gene namesi
Name:Necap2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi735063. Necap2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 263263Adaptin ear-binding coat-associated protein 2PRO_0000213073Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei181 – 1811PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6P756.
PRIDEiQ6P756.

PTM databases

iPTMnetiQ6P756.
PhosphoSiteiQ6P756.

Expressioni

Tissue specificityi

Expressed in brain, heart, kidney, liver and lung (at protein level).1 Publication

Gene expression databases

GenevisibleiQ6P756. RN.

Interactioni

Subunit structurei

Interacts with AP1G1 and AP2A1 components of the adapter protein complexes AP-1 and AP-2. Interacts with the GAE domain proteins GGA1, GGA2 and GGA3 (By similarity).By similarity

Protein-protein interaction databases

BioGridi255956. 5 interactions.
STRINGi10116.ENSRNOP00000011394.

Structurei

3D structure databases

ProteinModelPortaliQ6P756.
SMRiQ6P756. Positions 3-132.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi218 – 2214WXXF motif 1
Motifi238 – 2414WXXF motif 2

Domaini

The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself (By similarity).By similarity

Sequence similaritiesi

Belongs to the NECAP family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2500. Eukaryota.
ENOG41113PS. LUCA.
GeneTreeiENSGT00390000009359.
HOGENOMiHOG000231188.
HOVERGENiHBG060621.
InParanoidiQ6P756.
KOiK20069.
OMAiIQFEDAT.
OrthoDBiEOG76X61J.
PhylomeDBiQ6P756.
TreeFamiTF314482.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR012466. NECAP-1_N.
IPR011993. PH_dom-like.
[Graphical view]
PfamiPF07933. DUF1681. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6P756-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEESEYESVL CVKPEVHVYR IPPRATNRGY RASEWQLDQP SWSGRLRITA
60 70 80 90 100
KGKVAYIKLE DRTSGELFAQ APVDQFPGTA VESVTDSSRY FVIRIEDGNG
110 120 130 140 150
RRAFIGIGFG DRGDAFDFNV ALQDHFKWVK QQCEFAKQAQ NPDEGPKLDL
160 170 180 190 200
GFKEGQTIKI NIANMRKKEG AAGAPRTRPA SAGGLSLLPP PPGGKMSTLI
210 220 230 240 250
PPSGEQFSGG SLVQPVSGSG GATELWPQSK PAAAATADIW GDFTKSTGSP
260
SSQSQPGTGW VQF
Length:263
Mass (Da):28,405
Last modified:March 29, 2005 - v2
Checksum:iD9210ED9003CCDD1
GO
Isoform 2 (identifier: Q6P756-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: MEESEYESVL...DRTSGELFAQ → MSTASRRGPP...PTSSWRTGPL

Show »
Length:241
Mass (Da):25,324
Checksum:i4E829A73C42C1E52
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7070MEESE…ELFAQ → MSTASRRGPPTVVTGPQNGN WTSHPGVAGCGSLRKGRWPT SSWRTGPL in isoform 2. 1 PublicationVSP_013237Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC061826 mRNA. Translation: AAH61826.1.
CK366762 mRNA. No translation available.
CB724981 mRNA. No translation available.
RefSeqiNP_954527.1. NM_199096.1.
XP_006239241.1. XM_006239179.2. [Q6P756-1]
UniGeneiRn.56102.

Genome annotation databases

EnsembliENSRNOT00000011394; ENSRNOP00000011394; ENSRNOG00000008427. [Q6P756-1]
GeneIDi298598.
KEGGirno:298598.
UCSCiRGD:735063. rat. [Q6P756-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC061826 mRNA. Translation: AAH61826.1.
CK366762 mRNA. No translation available.
CB724981 mRNA. No translation available.
RefSeqiNP_954527.1. NM_199096.1.
XP_006239241.1. XM_006239179.2. [Q6P756-1]
UniGeneiRn.56102.

3D structure databases

ProteinModelPortaliQ6P756.
SMRiQ6P756. Positions 3-132.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi255956. 5 interactions.
STRINGi10116.ENSRNOP00000011394.

PTM databases

iPTMnetiQ6P756.
PhosphoSiteiQ6P756.

Proteomic databases

PaxDbiQ6P756.
PRIDEiQ6P756.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011394; ENSRNOP00000011394; ENSRNOG00000008427. [Q6P756-1]
GeneIDi298598.
KEGGirno:298598.
UCSCiRGD:735063. rat. [Q6P756-1]

Organism-specific databases

CTDi55707.
RGDi735063. Necap2.

Phylogenomic databases

eggNOGiKOG2500. Eukaryota.
ENOG41113PS. LUCA.
GeneTreeiENSGT00390000009359.
HOGENOMiHOG000231188.
HOVERGENiHBG060621.
InParanoidiQ6P756.
KOiK20069.
OMAiIQFEDAT.
OrthoDBiEOG76X61J.
PhylomeDBiQ6P756.
TreeFamiTF314482.

Miscellaneous databases

PROiQ6P756.

Gene expression databases

GenevisibleiQ6P756. RN.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR012466. NECAP-1_N.
IPR011993. PH_dom-like.
[Graphical view]
PfamiPF07933. DUF1681. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Prostate.
  2. "Identification and analysis of plasticity-induced late-response genes."
    Hong S.J., Li H., Becker K.G., Dawson V.L., Dawson T.M.
    Proc. Natl. Acad. Sci. U.S.A. 101:2145-2150(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-182 (ISOFORM 1).
  3. "Identification of a family of endocytic proteins that define a new alpha-adaptin ear-binding motif."
    Ritter B., Philie J., Girard M., Tung E.C., Blondeau F., McPherson P.S.
    EMBO Rep. 4:1089-1095(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiNECP2_RAT
AccessioniPrimary (citable) accession number: Q6P756
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: July 6, 2016
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.