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Protein

Adaptin ear-binding coat-associated protein 2

Gene

Necap2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in endocytosis.By similarity

GO - Biological processi

Keywordsi

Biological processEndocytosis, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-8856825 Cargo recognition for clathrin-mediated endocytosis
R-RNO-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Adaptin ear-binding coat-associated protein 2
Short name:
NECAP-2
Gene namesi
Name:Necap2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi735063 Necap2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002130731 – 263Adaptin ear-binding coat-associated protein 2Add BLAST263

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei181PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6P756
PRIDEiQ6P756

PTM databases

iPTMnetiQ6P756
PhosphoSitePlusiQ6P756

Expressioni

Tissue specificityi

Expressed in brain, heart, kidney, liver and lung (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000008427
GenevisibleiQ6P756 RN

Interactioni

Subunit structurei

Interacts with AP1G1 and AP2A1 components of the adapter protein complexes AP-1 and AP-2. Interacts with the GAE domain proteins GGA1, GGA2 and GGA3 (By similarity).By similarity

Protein-protein interaction databases

BioGridi255956, 5 interactors
STRINGi10116.ENSRNOP00000011394

Structurei

3D structure databases

ProteinModelPortaliQ6P756
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi218 – 221WXXF motif 14
Motifi238 – 241WXXF motif 24

Domaini

The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself (By similarity).By similarity

Sequence similaritiesi

Belongs to the NECAP family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2500 Eukaryota
ENOG41113PS LUCA
GeneTreeiENSGT00390000009359
HOGENOMiHOG000231188
HOVERGENiHBG060621
InParanoidiQ6P756
KOiK20069
OMAiVSWPQPK
OrthoDBiEOG091G0PZV
PhylomeDBiQ6P756
TreeFamiTF314482

Family and domain databases

CDDicd13228 PHear_NECAP, 1 hit
Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR012466 NECAP_PHear
IPR011993 PH-like_dom_sf
PfamiView protein in Pfam
PF07933 DUF1681, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6P756-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEESEYESVL CVKPEVHVYR IPPRATNRGY RASEWQLDQP SWSGRLRITA
60 70 80 90 100
KGKVAYIKLE DRTSGELFAQ APVDQFPGTA VESVTDSSRY FVIRIEDGNG
110 120 130 140 150
RRAFIGIGFG DRGDAFDFNV ALQDHFKWVK QQCEFAKQAQ NPDEGPKLDL
160 170 180 190 200
GFKEGQTIKI NIANMRKKEG AAGAPRTRPA SAGGLSLLPP PPGGKMSTLI
210 220 230 240 250
PPSGEQFSGG SLVQPVSGSG GATELWPQSK PAAAATADIW GDFTKSTGSP
260
SSQSQPGTGW VQF
Length:263
Mass (Da):28,405
Last modified:March 29, 2005 - v2
Checksum:iD9210ED9003CCDD1
GO
Isoform 2 (identifier: Q6P756-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: MEESEYESVL...DRTSGELFAQ → MSTASRRGPP...PTSSWRTGPL

Show »
Length:241
Mass (Da):25,324
Checksum:i4E829A73C42C1E52
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0132371 – 70MEESE…ELFAQ → MSTASRRGPPTVVTGPQNGN WTSHPGVAGCGSLRKGRWPT SSWRTGPL in isoform 2. 1 PublicationAdd BLAST70

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC061826 mRNA Translation: AAH61826.1
CK366762 mRNA No translation available.
CB724981 mRNA No translation available.
RefSeqiNP_954527.1, NM_199096.1
XP_006239241.1, XM_006239179.3 [Q6P756-1]
UniGeneiRn.56102

Genome annotation databases

EnsembliENSRNOT00000011394; ENSRNOP00000011394; ENSRNOG00000008427 [Q6P756-1]
GeneIDi298598
KEGGirno:298598
UCSCiRGD:735063 rat [Q6P756-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNECP2_RAT
AccessioniPrimary (citable) accession number: Q6P756
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: March 28, 2018
This is version 94 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health