Reviewed, UniProtKB/Swiss-Prot Q6P6V1 (GLT11_RAT)

Names and origin

Protein names

Recommended name:
    Polypeptide N-acetylgalactosaminyltransferase 11
    EC=2.4.1.41
Alternative name(s):
    Polypeptide GalNAc transferase 11
      Short name=pp-GaNTase 11
      Short name=GalNAc-T11
    Protein-UDP acetylgalactosaminyltransferase 11
    UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 11

Gene names

Name:

Galnt11

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	608 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Displays the same enzyme activity toward Muc1, Muc4.1, and EA2 than GALNT1. Does not appear to be involved in glycosylation of erythropoietin By similarity.
Catalytic activity	UDP-N-acetyl-D-galactosamine + polypeptide = UDP + N-acetyl-D-galactosaminyl-polypeptide.
Cofactor	Manganese By similarity. Calcium By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Domain	There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding By similarity. The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity By similarity.
Sequence similarities	Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. Contains 1 ricin B-type lectin domain.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Calcium Lectin Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond
Gene Ontology (GO)
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW polypeptide N-acetylgalactosaminyltransferase activity Inferred from electronic annotation. Source: EC sugar binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 608	608	Polypeptide N-acetylgalactosaminyltransferase 11		PRO_0000059127
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 29	23	Signal-anchor for type II membrane protein Potential
	Topological domain	30 – 608	579	Lumenal Potential
	Domain	476 – 607	132	Ricin B-type lectin
	Region	150 – 261	112	Catalytic subdomain A
	Region	319 – 381	63	Catalytic subdomain B
Amino acid modifications
	Disulfide bond	493 ↔ 512		By similarity
	Disulfide bond	536 ↔ 553		By similarity
	Disulfide bond	578 ↔ 596		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6P6V1-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: ED1295AC1EB35751	FASTA	608	69,039
	10 20 30 40 50 60 MGSVTIRYFC YGCLFTSATW TVLLFIYFNF SEVTQPLRNV PIKGSGPHGP FPKKFYPRFT 70 80 90 100 110 120 RGPGRVLEPQ FKANRMDDLM NNNIEDPDKG LSKSSSELGM IFNERDQELR DLGYQKHAFN 130 140 150 160 170 180 MLISNRLGYH RDVPDTRNAE CRGKSYPTDL PTASVVICFY NEAFSALLRT VHSVVDRTPA 190 200 210 220 230 240 HLLHEIILVD DSSDFDDLKG ELDEYIQRYL PAKVKVIRNM KREGLIRGRM IGAAHATGEV 250 260 270 280 290 300 LVFLDSHCEV NVMWLQPLLA IILEDPHTVV CPVIDIISAD TLAYSSSPVV RGGFNWGLHF 310 320 330 340 350 360 KWDLVPVSDL GGADSATAPI RSPTMAGGLF AMNRQYFNDL GQYDSGMDIW GGENLEISFR 370 380 390 400 410 420 IWMCGGKLFI IPCSRVGHIF RKRRPYGSPE GQDTMTHNSL RLAHVWLDEY KEQYFSLRPD 430 440 450 460 470 480 LKTKSFGNIS ERVELRKKLG CQSFKWYLDN VYPEMQVSGP KARLQQPVFI NRGPKRPRVL 490 500 510 520 530 540 LRGRLYHLQT NKCLVAQGRS SQKGGLVLLK ACDYGDPTQV WIYNEEHELI LNNLLCLDMS 550 560 570 580 590 600 ETRSSDPPRL MKCHGSGGSQ QWTFGKNNRL YQVSVGQCLR VVDQMDQKGY VGMAICDGSS SQQWRLEG

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC062004 mRNA. Translation: AAH62004.1.
IPI	IPI00400738.
RefSeq	NP_955425.1.
UniGene	Rn.211885
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	CBM13. Carbohydrate-Binding Module Family 13. GT27. Glycosyltransferase Family 27.
Proteomic databases
PRIDE	Q6P6V1.
Genome annotation databases
Ensembl	ENSRNOT00000011814; ENSRNOP00000011815; ENSRNOG00000008117; Rattus norvegicus. [Genome view]
GeneID	311952.
KEGG	rno:311952.
UCSC	NM_199393. rat.
Organism-specific databases
CTD	311952.
RGD	735097. Galnt11.
Phylogenomic databases
HOVERGEN	Q6P6V1.
OMA	GMIFNER.
Enzyme and pathway databases
BRENDA	2.4.1.41. 248.
Gene expression databases
ArrayExpress	Q6P6V1.
Genevestigator	Q6P6V1.
GermOnline	ENSRNOG00000008117. Rattus norvegicus.
Family and domain databases
InterPro	IPR001173. Glyco_trans_2. IPR000772. Ricin_B_lectin. [Graphical view]
Pfam	PF00535. Glycos_transf_2. 1 hit. PF00652. Ricin_B_lectin. 1 hit. [Graphical view]
SMART	SM00458. RICIN. 1 hit. [Graphical view]
PROSITE	PS50231. RICIN_B_LECTIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	664505.

Entry information

Entry name

GLT11_RAT

Accession

Primary (citable) accession number: Q6P6V1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)