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Q6P6V0 (G6PI_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucose-6-phosphate isomerase

Short name=GPI
EC=5.3.1.9
Alternative name(s):
Autocrine motility factor
Short name=AMF
Neuroleukin
Short name=NLK
Phosphoglucose isomerase
Short name=PGI
Phosphohexose isomerase
Short name=PHI
Gene names
Name:Gpi
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length558 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons By similarity. HAMAP-Rule MF_00473

Catalytic activity

D-glucose 6-phosphate = D-fructose 6-phosphate. HAMAP-Rule MF_00473

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4. HAMAP-Rule MF_00473

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00473

Subcellular location

Secreted By similarity. Cytoplasm Ref.2.

Post-translational modification

ISGylated By similarity. HAMAP-Rule MF_00473

Sequence similarities

Belongs to the GPI family.

Ontologies

Keywords
   Biological processAngiogenesis
Gluconeogenesis
Glycolysis
   Cellular componentCytoplasm
Secreted
   Molecular functionCytokine
Growth factor
Isomerase
   PTMAcetylation
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaldehyde catabolic process

Inferred from mutant phenotype PubMed 16583131. Source: RGD

angiogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

carbohydrate metabolic process

Inferred from direct assay PubMed 1834654. Source: RGD

gluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

glucose 6-phosphate metabolic process

Inferred from direct assay PubMed 2709006. Source: RGD

glycolysis

Inferred from electronic annotation. Source: UniProtKB-UniPathway

learning or memory

Inferred from expression pattern PubMed 11902125. Source: RGD

methylglyoxal biosynthetic process

Inferred from mutant phenotype PubMed 16583131. Source: RGD

negative regulation of cysteine-type endopeptidase activity involved in apoptotic process

Inferred from mutant phenotype PubMed 12615053. Source: RGD

negative regulation of neuron apoptotic process

Inferred from mutant phenotype PubMed 12615053. Source: RGD

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 16583131. Source: RGD

cytosol

Inferred from direct assay PubMed 1834654. Source: RGD

extracellular space

Inferred from electronic annotation. Source: UniProtKB-KW

neuron projection

Inferred from direct assay PubMed 2990810. Source: RGD

nucleus

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionglucose-6-phosphate isomerase activity

Inferred from direct assay PubMed 1834654PubMed 2709006. Source: RGD

intramolecular transferase activity

Inferred from mutant phenotype PubMed 16583131. Source: RGD

monosaccharide binding

Inferred from physical interaction PubMed 2709006. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 558557Glucose-6-phosphate isomerase HAMAP-Rule MF_00473
PRO_0000349123

Sites

Active site3581Proton donor By similarity
Active site3891 By similarity
Active site5191 By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue121N6-acetyllysine By similarity
Modified residue1421N6-acetyllysine By similarity
Modified residue1851Phosphoserine; by CK2 By similarity
Modified residue4541N6-malonyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6P6V0 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 2550ACB874E0302C

FASTA55862,827
        10         20         30         40         50         60 
MAALTRNPEF QKLLEWHRAN SANLKLRELF EADPERFNHF SLNLNTNHGH ILLDYSKNLV 

        70         80         90        100        110        120 
NKEVLHMLVD LAKSRGVEAA RDNMFSGLKI NSTEDRAVLH VALRNRSNRS IMMDGKDVMP 

       130        140        150        160        170        180 
EVNKVLDKMK SFCQRVRSGD WKGYTGKAIT DIINIGIGGS DLGPLMVTEA LKPYSKGGPR 

       190        200        210        220        230        240 
VWFVSNIDGT HIAKTLANLN PESSLFIIAS KTFTTQETIT NAETAKEWFL QAAKDPSAVA 

       250        260        270        280        290        300 
KHFVALSTNT DKVKEFGIDP KNMFEFWDWV GGRYSLWSAI GLSIALHVGF DHFEQLLSGA 

       310        320        330        340        350        360 
HWMDQHFMKT PLDKNAPVLL ALLGIWYINF YGCETHAMLP YDQYMHRFAA YFQQGDMESN 

       370        380        390        400        410        420 
GKYITKSGAR VDYQTGPIVW GEPGTNGQHA FYQLIHQGTK MIPCDFLIPV QTQHPIRNGL 

       430        440        450        460        470        480 
HHKILLANFL AQTEALMKGK SPEEARKELQ AAGKSPEELE KLLPHKVFEG NRPTNSIVFT 

       490        500        510        520        530        540 
KLTPFILGAL IAMYEHKIFV QGIIWDINSF DQWGVELGKQ LAKKIEPELD GSSAVTSHDS 

       550 
STNGLIGFIK LQRDTKID 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.
[2]"Proteome profile of the mature rat olfactory bulb."
Maurya D.K., Sundaram C.S., Bhargava P.
Proteomics 9:2593-2599(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC062005 mRNA. Translation: AAH62005.1.
RefSeqNP_997475.1. NM_207592.1.
UniGeneRn.84435.

3D structure databases

ProteinModelPortalQ6P6V0.
SMRQ6P6V0. Positions 1-557.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid253982. 1 interaction.
IntActQ6P6V0. 2 interactions.
STRING10116.ENSRNOP00000029515.

PTM databases

PhosphoSiteQ6P6V0.

2D gel databases

World-2DPAGE0004:Q6P6V0.

Proteomic databases

PaxDbQ6P6V0.
PRIDEQ6P6V0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000032613; ENSRNOP00000029515; ENSRNOG00000023150.
GeneID292804.
KEGGrno:292804.
UCSCRGD:2727. rat.

Organism-specific databases

CTD2821.
RGD2727. Gpi.

Phylogenomic databases

eggNOGCOG0166.
GeneTreeENSGT00390000000707.
HOGENOMHOG000261370.
HOVERGENHBG002877.
InParanoidQ6P6V0.
KOK01810.
OMALKMHFVS.
OrthoDBEOG7FXZXV.
PhylomeDBQ6P6V0.
TreeFamTF300436.

Enzyme and pathway databases

SABIO-RKQ6P6V0.
UniPathwayUPA00109; UER00181.

Gene expression databases

GenevestigatorQ6P6V0.

Family and domain databases

Gene3D1.10.1390.10. 1 hit.
HAMAPMF_00473. G6P_isomerase.
InterProIPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERPTHR11469. PTHR11469. 1 hit.
PfamPF00342. PGI. 1 hit.
[Graphical view]
PRINTSPR00662. G6PISOMERASE.
PROSITEPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio634825.
PROQ6P6V0.

Entry information

Entry nameG6PI_RAT
AccessionPrimary (citable) accession number: Q6P6V0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways