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Protein

Glucose-6-phosphate isomerase

Gene

Gpi

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons (By similarity).By similarity

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.By similarity

Pathway:iglycolysis

This protein is involved in step 2 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.By similarity
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (Gpi)
  3. ATP-dependent 6-phosphofructokinase (Pfkm), ATP-dependent 6-phosphofructokinase, liver type (Pfkl), ATP-dependent 6-phosphofructokinase, platelet type (Pfkp), ATP-dependent 6-phosphofructokinase (Pfkp), ATP-dependent 6-phosphofructokinase (Pfkl), ATP-dependent 6-phosphofructokinase, muscle type (Pfkm), ATP-dependent 6-phosphofructokinase (Pfkp)
  4. Fructose-bisphosphate aldolase (LOC100912010), Fructose-bisphosphate aldolase, Fructose-bisphosphate aldolase, Fructose-bisphosphate aldolase, Fructose-bisphosphate aldolase (Aldoart2), Fructose-bisphosphate aldolase A (Aldoa), Fructose-bisphosphate aldolase B (Aldob), Fructose-bisphosphate aldolase C (Aldoc), Fructose-bisphosphate aldolase (Aldob)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei358 – 3581Proton donorBy similarity
Active sitei389 – 3891By similarity
Active sitei519 – 5191By similarity

GO - Molecular functioni

  • glucose-6-phosphate isomerase activity Source: RGD
  • intramolecular transferase activity Source: RGD
  • monosaccharide binding Source: RGD

GO - Biological processi

  • aldehyde catabolic process Source: RGD
  • angiogenesis Source: UniProtKB-KW
  • carbohydrate metabolic process Source: RGD
  • gluconeogenesis Source: UniProtKB-KW
  • glucose 6-phosphate metabolic process Source: RGD
  • glycolytic process Source: UniProtKB-UniPathway
  • learning or memory Source: RGD
  • methylglyoxal biosynthetic process Source: RGD
  • negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: RGD
  • negative regulation of neuron apoptotic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Growth factor, Isomerase

Keywords - Biological processi

Angiogenesis, Gluconeogenesis, Glycolysis

Enzyme and pathway databases

ReactomeiREACT_331968. Gluconeogenesis.
REACT_342181. Glycolysis.
REACT_358301. TP53 Regulates Metabolic Genes.
SABIO-RKQ6P6V0.
UniPathwayiUPA00109; UER00181.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate isomeraseBy similarity (EC:5.3.1.9)
Short name:
GPIBy similarity
Alternative name(s):
Autocrine motility factor
Short name:
AMF
NeuroleukinBy similarity
Short name:
NLKBy similarity
Phosphoglucose isomeraseBy similarity
Short name:
PGIBy similarity
Phosphohexose isomeraseBy similarity
Short name:
PHIBy similarity
Gene namesi
Name:GpiImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi2727. Gpi.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytosol Source: RGD
  • extracellular exosome Source: Ensembl
  • extracellular space Source: UniProtKB-KW
  • neuron projection Source: RGD
  • nucleoplasm Source: Ensembl
  • plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 558557Glucose-6-phosphate isomeraseBy similarityPRO_0000349123Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei12 – 121N6-acetyllysineBy similarity
Modified residuei142 – 1421N6-acetyllysineBy similarity
Modified residuei185 – 1851Phosphoserine; by CK2By similarity
Modified residuei454 – 4541N6-malonyllysineBy similarity

Post-translational modificationi

ISGylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6P6V0.
PRIDEiQ6P6V0.

2D gel databases

World-2DPAGE0004:Q6P6V0.

PTM databases

PhosphoSiteiQ6P6V0.

Expressioni

Gene expression databases

GenevisibleiQ6P6V0. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi253982. 1 interaction.
IntActiQ6P6V0. 2 interactions.
STRINGi10116.ENSRNOP00000029515.

Structurei

3D structure databases

ProteinModelPortaliQ6P6V0.
SMRiQ6P6V0. Positions 1-557.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GPI family.Sequence Analysis

Phylogenomic databases

eggNOGiCOG0166.
GeneTreeiENSGT00390000000707.
HOGENOMiHOG000261370.
HOVERGENiHBG002877.
InParanoidiQ6P6V0.
KOiK01810.
OMAiCETQAML.
OrthoDBiEOG7FXZXV.
PhylomeDBiQ6P6V0.
TreeFamiTF300436.

Family and domain databases

Gene3Di1.10.1390.10. 1 hit.
HAMAPiMF_00473. G6P_isomerase.
InterProiIPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 1 hit.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6P6V0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALTRNPEF QKLLEWHRAN SANLKLRELF EADPERFNHF SLNLNTNHGH
60 70 80 90 100
ILLDYSKNLV NKEVLHMLVD LAKSRGVEAA RDNMFSGLKI NSTEDRAVLH
110 120 130 140 150
VALRNRSNRS IMMDGKDVMP EVNKVLDKMK SFCQRVRSGD WKGYTGKAIT
160 170 180 190 200
DIINIGIGGS DLGPLMVTEA LKPYSKGGPR VWFVSNIDGT HIAKTLANLN
210 220 230 240 250
PESSLFIIAS KTFTTQETIT NAETAKEWFL QAAKDPSAVA KHFVALSTNT
260 270 280 290 300
DKVKEFGIDP KNMFEFWDWV GGRYSLWSAI GLSIALHVGF DHFEQLLSGA
310 320 330 340 350
HWMDQHFMKT PLDKNAPVLL ALLGIWYINF YGCETHAMLP YDQYMHRFAA
360 370 380 390 400
YFQQGDMESN GKYITKSGAR VDYQTGPIVW GEPGTNGQHA FYQLIHQGTK
410 420 430 440 450
MIPCDFLIPV QTQHPIRNGL HHKILLANFL AQTEALMKGK SPEEARKELQ
460 470 480 490 500
AAGKSPEELE KLLPHKVFEG NRPTNSIVFT KLTPFILGAL IAMYEHKIFV
510 520 530 540 550
QGIIWDINSF DQWGVELGKQ LAKKIEPELD GSSAVTSHDS STNGLIGFIK

LQRDTKID
Length:558
Mass (Da):62,827
Last modified:July 5, 2004 - v1
Checksum:i2550ACB874E0302C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC062005 mRNA. Translation: AAH62005.1.
RefSeqiNP_997475.1. NM_207592.1.
UniGeneiRn.84435.

Genome annotation databases

EnsembliENSRNOT00000032613; ENSRNOP00000029515; ENSRNOG00000023150.
GeneIDi292804.
KEGGirno:292804.
UCSCiRGD:2727. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC062005 mRNA. Translation: AAH62005.1.
RefSeqiNP_997475.1. NM_207592.1.
UniGeneiRn.84435.

3D structure databases

ProteinModelPortaliQ6P6V0.
SMRiQ6P6V0. Positions 1-557.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi253982. 1 interaction.
IntActiQ6P6V0. 2 interactions.
STRINGi10116.ENSRNOP00000029515.

PTM databases

PhosphoSiteiQ6P6V0.

2D gel databases

World-2DPAGE0004:Q6P6V0.

Proteomic databases

PaxDbiQ6P6V0.
PRIDEiQ6P6V0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000032613; ENSRNOP00000029515; ENSRNOG00000023150.
GeneIDi292804.
KEGGirno:292804.
UCSCiRGD:2727. rat.

Organism-specific databases

CTDi2821.
RGDi2727. Gpi.

Phylogenomic databases

eggNOGiCOG0166.
GeneTreeiENSGT00390000000707.
HOGENOMiHOG000261370.
HOVERGENiHBG002877.
InParanoidiQ6P6V0.
KOiK01810.
OMAiCETQAML.
OrthoDBiEOG7FXZXV.
PhylomeDBiQ6P6V0.
TreeFamiTF300436.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00181.
ReactomeiREACT_331968. Gluconeogenesis.
REACT_342181. Glycolysis.
REACT_358301. TP53 Regulates Metabolic Genes.
SABIO-RKQ6P6V0.

Miscellaneous databases

NextBioi634825.
PROiQ6P6V0.

Gene expression databases

GenevisibleiQ6P6V0. RN.

Family and domain databases

Gene3Di1.10.1390.10. 1 hit.
HAMAPiMF_00473. G6P_isomerase.
InterProiIPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 1 hit.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: ProstateImported.
  2. "Proteome profile of the mature rat olfactory bulb."
    Maurya D.K., Sundaram C.S., Bhargava P.
    Proteomics 9:2593-2599(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiG6PI_RAT
AccessioniPrimary (citable) accession number: Q6P6V0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: July 5, 2004
Last modified: June 24, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.