Q6P6V0 (G6PI_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucose-6-phosphate isomerase
Short name=GPI
EC=5.3.1.9

Alternative name(s):
Autocrine motility factor
Short name=AMF
Neuroleukin
Short name=NLK
Phosphoglucose isomerase
Short name=PGI
Phosphohexose isomerase
Short name=PHI

Gene names

Name:

Gpi

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons By similarity.
Catalytic activity	D-glucose 6-phosphate = D-fructose 6-phosphate. UniProtKB P06745
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4. UniProtKB P06745
Subunit structure	Homodimer By similarity. UniProtKB P06745
Subcellular location	Secreted By similarity. Cytoplasm Ref.2.
Post-translational modification	ISGylated By similarity.
Sequence similarities	Belongs to the GPI family.

Ontologies

Keywords
Biological process	Angiogenesis Gluconeogenesis Glycolysis
Cellular component	Cytoplasm Secreted
Molecular function	Cytokine Growth factor Isomerase
PTM	Acetylation Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aldehyde catabolic process Inferred from mutant phenotype. Source: RGD angiogenesis Inferred from electronic annotation. Source: UniProtKB-KW gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glucose 6-phosphate metabolic process Inferred from direct assay. Source: RGD glycolysis Inferred from electronic annotation. Source: UniProtKB-KW learning or memory Inferred from expression pattern. Source: RGD methylglyoxal biosynthetic process Inferred from mutant phenotype. Source: RGD negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from mutant phenotype. Source: RGD negative regulation of neuron apoptosis Inferred from mutant phenotype. Source: RGD
Cellular component	cytosol Inferred from direct assay. Source: RGD extracellular space Inferred from electronic annotation. Source: UniProtKB-KW soluble fraction Inferred from direct assay. Source: RGD synaptosome Inferred from direct assay. Source: RGD
Molecular function	cytokine activity Inferred from electronic annotation. Source: UniProtKB-KW glucose-6-phosphate isomerase activity Inferred from direct assay. Source: RGD growth factor activity Inferred from electronic annotation. Source: UniProtKB-KW intramolecular transferase activity Inferred from mutant phenotype. Source: RGD monosaccharide binding Inferred from physical interaction. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity UniProtKB P06744
	Chain	2 – 558	557	Glucose-6-phosphate isomerase UniProtKB P06744		PRO_0000349123
Sites
	Active site	358	1	Proton donor By similarity
	Active site	389	1	By similarity UniProtKB P06745
	Active site	519	1	By similarity UniProtKB P06745
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity UniProtKB P06744
	Modified residue	12	1	N6-acetyllysine By similarity
	Modified residue	142	1	N6-acetyllysine By similarity
	Modified residue	185	1	Phosphoserine; by CK2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6P6V0 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 2550ACB874E0302C	FASTA	558	62,827
	10 20 30 40 50 60 MAALTRNPEF QKLLEWHRAN SANLKLRELF EADPERFNHF SLNLNTNHGH ILLDYSKNLV 70 80 90 100 110 120 NKEVLHMLVD LAKSRGVEAA RDNMFSGLKI NSTEDRAVLH VALRNRSNRS IMMDGKDVMP 130 140 150 160 170 180 EVNKVLDKMK SFCQRVRSGD WKGYTGKAIT DIINIGIGGS DLGPLMVTEA LKPYSKGGPR 190 200 210 220 230 240 VWFVSNIDGT HIAKTLANLN PESSLFIIAS KTFTTQETIT NAETAKEWFL QAAKDPSAVA 250 260 270 280 290 300 KHFVALSTNT DKVKEFGIDP KNMFEFWDWV GGRYSLWSAI GLSIALHVGF DHFEQLLSGA 310 320 330 340 350 360 HWMDQHFMKT PLDKNAPVLL ALLGIWYINF YGCETHAMLP YDQYMHRFAA YFQQGDMESN 370 380 390 400 410 420 GKYITKSGAR VDYQTGPIVW GEPGTNGQHA FYQLIHQGTK MIPCDFLIPV QTQHPIRNGL 430 440 450 460 470 480 HHKILLANFL AQTEALMKGK SPEEARKELQ AAGKSPEELE KLLPHKVFEG NRPTNSIVFT 490 500 510 520 530 540 KLTPFILGAL IAMYEHKIFV QGIIWDINSF DQWGVELGKQ LAKKIEPELD GSSAVTSHDS 550 STNGLIGFIK LQRDTKID

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[2]	"Proteome profile of the mature rat olfactory bulb." Maurya D.K., Sundaram C.S., Bhargava P. Proteomics 9:2593-2599(2009) [PubMed: 19343716] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC062005 mRNA. Translation: AAH62005.1.
IPI	IPI00364311.
RefSeq	NP_997475.1. NM_207592.1.
UniGene	Rn.84435.
3D structure databases
HSSP	HSSP built from PDB template 2CXN based on UniProtKB Q3TEE7.
ProteinModelPortal	Q6P6V0.
SMR	Q6P6V0. Positions 1-557.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6P6V0. 1 interaction.
STRING	Q6P6V0.
PTM databases
PhosphoSite	Q6P6V0.
2D gel databases
World-2DPAGE	0004:Q6P6V0.
Proteomic databases
PRIDE	Q6P6V0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000032613; ENSRNOP00000029515; ENSRNOG00000023150.
GeneID	292804.
KEGG	rno:292804.
NMPDR	fig|10116.3.peg.1584.
UCSC	NM_207592. rat.
Organism-specific databases
CTD	2821.
RGD	2727. Gpi.
Phylogenomic databases
eggNOG	roNOG05642.
GeneTree	ENSGT00390000000707.
HOVERGEN	HBG002877.
InParanoid	Q6P6V0.
OMA	GPKIVSQ.
OrthoDB	EOG44MXRS.
PhylomeDB	Q6P6V0.
Gene expression databases
ArrayExpress	Q6P6V0.
Genevestigator	Q6P6V0.
Family and domain databases
InterPro	IPR001672. G6P_Isomerase. IPR023096. G6P_Isomerase_C. IPR018189. Phosphoglucose_isomerase_CS. [Graphical view]
Gene3D	G3DSA:1.10.1390.10. G6P_Isomerase_C. 1 hit.
KO	K01810.
PANTHER	PTHR11469. G6P_Isomerase. 1 hit.
Pfam	PF00342. PGI. 1 hit. [Graphical view]
PRINTS	PR00662. G6PISOMERASE.
PROSITE	PS00765. P_GLUCOSE_ISOMERASE_1. 1 hit. PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit. PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	634825.

Entry information

Entry name

G6PI_RAT

Accession

Primary (citable) accession number: Q6P6V0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program