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Protein

Occludin

Gene

Ocln

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. May be involved in the organization of actin in endothelial cells.1 Publication

GO - Biological processi

  • bicellular tight junction assembly Source: Ensembl
  • cellular response to tumor necrosis factor Source: RGD
  • response to interleukin-18 Source: RGD
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Occludin
Gene namesi
Name:Ocln
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi620089. Ocln.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein Sequence analysis
  • Cell junctiontight junction By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 66CytoplasmicSequence analysisAdd BLAST66
Transmembranei67 – 87HelicalSequence analysisAdd BLAST21
Topological domaini88 – 108ExtracellularSequence analysisAdd BLAST21
Transmembranei109 – 129HelicalSequence analysisAdd BLAST21
Topological domaini130 – 140CytoplasmicSequence analysisAdd BLAST11
Transmembranei141 – 161HelicalSequence analysisAdd BLAST21
Topological domaini162 – 173ExtracellularSequence analysisAdd BLAST12
Transmembranei174 – 194HelicalSequence analysisAdd BLAST21
Topological domaini195 – 244CytoplasmicSequence analysisAdd BLAST50
Transmembranei245 – 265HelicalSequence analysisAdd BLAST21
Topological domaini266 – 523ExtracellularSequence analysisAdd BLAST258

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • apicolateral plasma membrane Source: Ensembl
  • bicellular tight junction Source: RGD
  • endocytic vesicle Source: Ensembl
  • integral component of membrane Source: UniProtKB-KW
  • lateral plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002361031 – 523OccludinAdd BLAST523

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei302PhosphoserineBy similarity1
Modified residuei305PhosphothreonineBy similarity1
Modified residuei313PhosphoserineBy similarity1
Modified residuei321PhosphoserineBy similarity1
Modified residuei340PhosphoserineBy similarity1
Modified residuei360PhosphoserineBy similarity1
Modified residuei369PhosphotyrosineBy similarity1
Modified residuei370PhosphoserineBy similarity1
Modified residuei371PhosphoserineCombined sources1
Modified residuei399PhosphotyrosineBy similarity1
Modified residuei403PhosphotyrosineBy similarity1
Modified residuei404Phosphothreonine; by PKC/PRKCHBy similarity1
Modified residuei405Phosphothreonine; by PKC/PRKCHBy similarity1
Modified residuei409PhosphoserineBy similarity1
Modified residuei491PhosphoserineBy similarity1

Post-translational modificationi

Dephosphorylated by PTPRJ.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6P6T5.
PRIDEiQ6P6T5.

PTM databases

iPTMnetiQ6P6T5.
PhosphoSitePlusiQ6P6T5.

Expressioni

Gene expression databases

BgeeiENSRNOG00000018297.
GenevisibleiQ6P6T5. RN.

Interactioni

Subunit structurei

Interacts with TJP1/ZO1 and with VAPA.By similarity

Protein-protein interaction databases

BioGridi249716. 2 interactors.
DIPiDIP-48891N.
STRINGi10116.ENSRNOP00000024674.

Structurei

3D structure databases

ProteinModelPortaliQ6P6T5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini60 – 269MARVELPROSITE-ProRule annotationAdd BLAST210

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili433 – 489Sequence analysisAdd BLAST57

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi309 – 312Poly-Pro4

Domaini

The C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity).By similarity

Sequence similaritiesi

Belongs to the ELL/occludin family.Curated
Contains 1 MARVEL domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IGK8. Eukaryota.
ENOG4110E0E. LUCA.
GeneTreeiENSGT00730000110989.
HOGENOMiHOG000233490.
HOVERGENiHBG004523.
InParanoidiQ6P6T5.
KOiK06088.
OMAiPERILWE.
TreeFamiTF326161.

Family and domain databases

InterProiIPR031176. ELL/occludin.
IPR008253. Marvel.
IPR002958. Occludin.
IPR010844. Occludin_ELL.
[Graphical view]
PANTHERiPTHR23288. PTHR23288. 1 hit.
PTHR23288:SF4. PTHR23288:SF4. 1 hit.
PfamiPF01284. MARVEL. 1 hit.
PF07303. Occludin_ELL. 1 hit.
[Graphical view]
PIRSFiPIRSF005993. Occludin. 1 hit.
PRINTSiPR01258. OCCLUDIN.
PROSITEiPS51225. MARVEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6P6T5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVRPFESPP PYRPDEFKPN HYAPSNDMYG GEMHVRPMLS QPAYSFYPED
60 70 80 90 100
EILHFYKWTS PPGVIRILSM LVIVMCIAVF ACVASTLAWD RAYGTGIFGG
110 120 130 140 150
SMNYPYGSGF GSYGGGFGGY GYGYGYGYGG YTDPRAAKGF LLAMAAFCFI
160 170 180 190 200
ASLVIFVTSV IRSGMSRTRR YYLIVIIVSA ILGIMVFIAT IVYIMGVNPT
210 220 230 240 250
AQASGSMYGS QIYTICSQFY TPGGTGLYVD QYLYHYCVVD PQEAIAIVLG
260 270 280 290 300
FMIIVAFALI IVFAVKTRRK MDRYDKSNIL WDKEHIYDEQ PPNVEEWVKN
310 320 330 340 350
VSAGTQDMPP PPSDYAERVD SPMAYSSNGK VNGKRSYPDS LYKSPPLVPE
360 370 380 390 400
VAQEIPLTLS VDDFRQPRYS SNDNLETPSK RTPTKGKAGK AKRTDPDHYE
410 420 430 440 450
TDYTTGGESC DELEEDWLRE YPPITSDQQR QLYKRNFDAG LQEYKSLLAE
460 470 480 490 500
LDEVNKELSR LDRELDDYRE ESEEYMAAAD EYNRLKQVKG SADYKSKKNY
510 520
CKQLKSKLSH IKRMVGDYDR RKT
Length:523
Mass (Da):59,186
Last modified:July 5, 2004 - v1
Checksum:iA31E007B58AEA5B5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti276K → R in BAA36681 (PubMed:11810420).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016425 mRNA. Translation: BAA36681.1.
BC062037 mRNA. Translation: AAH62037.1.
RefSeqiNP_112619.2. NM_031329.2.
XP_006231915.1. XM_006231853.3.
XP_006231916.1. XM_006231854.3.
XP_006231917.1. XM_006231855.3.
UniGeneiRn.31429.

Genome annotation databases

EnsembliENSRNOT00000024674; ENSRNOP00000024674; ENSRNOG00000018297.
ENSRNOT00000084667; ENSRNOP00000068691; ENSRNOG00000018297.
GeneIDi83497.
KEGGirno:83497.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016425 mRNA. Translation: BAA36681.1.
BC062037 mRNA. Translation: AAH62037.1.
RefSeqiNP_112619.2. NM_031329.2.
XP_006231915.1. XM_006231853.3.
XP_006231916.1. XM_006231854.3.
XP_006231917.1. XM_006231855.3.
UniGeneiRn.31429.

3D structure databases

ProteinModelPortaliQ6P6T5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249716. 2 interactors.
DIPiDIP-48891N.
STRINGi10116.ENSRNOP00000024674.

PTM databases

iPTMnetiQ6P6T5.
PhosphoSitePlusiQ6P6T5.

Proteomic databases

PaxDbiQ6P6T5.
PRIDEiQ6P6T5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024674; ENSRNOP00000024674; ENSRNOG00000018297.
ENSRNOT00000084667; ENSRNOP00000068691; ENSRNOG00000018297.
GeneIDi83497.
KEGGirno:83497.

Organism-specific databases

CTDi100506658.
RGDi620089. Ocln.

Phylogenomic databases

eggNOGiENOG410IGK8. Eukaryota.
ENOG4110E0E. LUCA.
GeneTreeiENSGT00730000110989.
HOGENOMiHOG000233490.
HOVERGENiHBG004523.
InParanoidiQ6P6T5.
KOiK06088.
OMAiPERILWE.
TreeFamiTF326161.

Miscellaneous databases

PROiQ6P6T5.

Gene expression databases

BgeeiENSRNOG00000018297.
GenevisibleiQ6P6T5. RN.

Family and domain databases

InterProiIPR031176. ELL/occludin.
IPR008253. Marvel.
IPR002958. Occludin.
IPR010844. Occludin_ELL.
[Graphical view]
PANTHERiPTHR23288. PTHR23288. 1 hit.
PTHR23288:SF4. PTHR23288:SF4. 1 hit.
PfamiPF01284. MARVEL. 1 hit.
PF07303. Occludin_ELL. 1 hit.
[Graphical view]
PIRSFiPIRSF005993. Occludin. 1 hit.
PRINTSiPR01258. OCCLUDIN.
PROSITEiPS51225. MARVEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOCLN_RAT
AccessioniPrimary (citable) accession number: Q6P6T5
Secondary accession number(s): Q9Z303
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.