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Protein

Echinoderm microtubule-associated protein-like 2

Gene

Eml2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Tubulin binding protein that inhibits microtubule nucleation and growth, resulting in shorter microtubules.By similarity

GO - Molecular functioni

  • catalytic activity Source: InterPro
  • microtubule binding Source: UniProtKB
  • protein C-terminus binding Source: RGD
  • receptor binding Source: RGD
  • tubulin binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Echinoderm microtubule-associated protein-like 2
Short name:
EMAP-2
Gene namesi
Name:Eml2
Synonyms:Emap2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi621066. Eml2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002843891 – 649Echinoderm microtubule-associated protein-like 2Add BLAST649

Proteomic databases

PaxDbiQ6P6T4.
PRIDEiQ6P6T4.

PTM databases

iPTMnetiQ6P6T4.
PhosphoSitePlusiQ6P6T4.

Expressioni

Tissue specificityi

Widely expressed in both brain and peripheral tissues, including brainstem and enrichment in the postsynaptic density, PSD.1 Publication

Gene expression databases

BgeeiENSRNOG00000030127.
GenevisibleiQ6P6T4. RN.

Interactioni

Subunit structurei

Binds unpolymerized tubulins via its WD repeat region (By similarity). Interacts with GRID2 and may also interact with GRID1.By similarity1 Publication

GO - Molecular functioni

  • microtubule binding Source: UniProtKB
  • protein C-terminus binding Source: RGD
  • receptor binding Source: RGD
  • tubulin binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000046691.

Structurei

3D structure databases

ProteinModelPortaliQ6P6T4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati103 – 152WD 1Add BLAST50
Repeati157 – 200WD 2Add BLAST44
Repeati203 – 242WD 3Add BLAST40
Repeati250 – 288WD 4Add BLAST39
Repeati292 – 331WD 5Add BLAST40
Repeati375 – 414WD 6Add BLAST40
Repeati416 – 455WD 7Add BLAST40
Repeati458 – 497WD 8Add BLAST40
Repeati504 – 543WD 9Add BLAST40
Repeati571 – 610WD 10Add BLAST40
Repeati616 – 649WD 11Add BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni10 – 649Tandem atypical propeller in EMLsBy similarityAdd BLAST640

Domaini

Contains a tandem atypical propeller in EMLs (TAPE) domain. The N-terminal beta-propeller is formed by canonical WD repeats; in contrast, the second beta-propeller contains one blade that is formed by discontinuous parts of the polypeptide chain (By similarity).By similarity

Sequence similaritiesi

Belongs to the WD repeat EMAP family.Curated
Contains 11 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2106. Eukaryota.
ENOG410XNTQ. LUCA.
GeneTreeiENSGT00550000074369.
HOGENOMiHOG000294061.
HOVERGENiHBG051470.
InParanoidiQ6P6T4.
KOiK18595.
OMAiDDANDHI.
PhylomeDBiQ6P6T4.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR033016. EML2.
IPR005108. HELP.
IPR011041. Quinoprot_gluc/sorb_DH.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR13720:SF32. PTHR13720:SF32. 1 hit.
PfamiPF03451. HELP. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view]
SMARTiSM00320. WD40. 11 hits.
[Graphical view]
SUPFAMiSSF50952. SSF50952. 1 hit.
SSF50998. SSF50998. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6P6T4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSFGTGKTK EVIFSMEEGS VKMFLRGRPV PMLIPDELAP TYSLDTRSEL
60 70 80 90 100
PSSRLKLDWV YGYRGRDCRA NLYLLPTGEV VYFVASVAVL YSVEEQRQRH
110 120 130 140 150
YLGHNDDIKC LAVHPDMVTI ATGQVAGTTK EGKPLPPHVR VWDSVSLSTL
160 170 180 190 200
HVLGLGVFDR AVCCVAFSKS NGGNLLCAVD ESNDHVLSVW DWAKESKVVD
210 220 230 240 250
SKCSNEAVLV ATFHPTDPNL LITCGKSHIY FWSLEGGNLS KRQGLFEKHE
260 270 280 290 300
KPKYVLCVTF LEGGDVVTGD SGGNLYVWGK GGNRITQEVL GAHDGGVFAL
310 320 330 340 350
CALRDGTLVS GGGRDRRVVL WGSDYSKVQE VEVPEDFGPV RTVAEGRGDT
360 370 380 390 400
LYVGTTRNSI LLGSVHTGFS LLVQGHVEEL WGLATHPSRA QFVSCGQDKL
410 420 430 440 450
VHLWSSETHQ PVWSRSIEDP ARSAGFHPSG SVLAVGTVTG RWLLLDTDTR
460 470 480 490 500
DLVAIHTDGN EQISVVSFSP DGAYLAVGSH DNLVYVYTVD QGGRKVSRLG
510 520 530 540 550
KCSGHSSFIT HLDWAQDSTC FVTNSGDYEI LYWDAATCKQ ITSADTVRNV
560 570 580 590 600
QWATATCVLG FGVFGIWPEG ADGTDINAVA RSHDGNLLVS ADDFGKVHLF
610 620 630 640
SYPCCQPRAL SHKYGGHSSH VTNVAFLWDD SMVLTTGGKD TSVLQWRVA
Length:649
Mass (Da):70,711
Last modified:July 5, 2004 - v1
Checksum:iBCAEEA4699EEB9A9
GO
Isoform 2 (identifier: Q6P6T4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: Missing.

Note: No experimental confirmation available.
Show »
Length:634
Mass (Da):69,110
Checksum:i499137428649435D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti311 – 312GG → VW in AAL33537 (PubMed:11829466).Curated2
Sequence conflicti491 – 492QG → HC in AAL33537 (PubMed:11829466).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0244811 – 15Missing in isoform 2. 1 PublicationAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF335571 mRNA. Translation: AAL33537.1.
BC062038 mRNA. Translation: AAH62038.1.
PIRiJC7808.
RefSeqiNP_620276.1. NM_138921.1.
UniGeneiRn.76362.

Genome annotation databases

EnsembliENSRNOT00000041215; ENSRNOP00000046691; ENSRNOG00000030127. [Q6P6T4-2]
ENSRNOT00000078336; ENSRNOP00000075212; ENSRNOG00000030127. [Q6P6T4-1]
GeneIDi192360.
KEGGirno:192360.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF335571 mRNA. Translation: AAL33537.1.
BC062038 mRNA. Translation: AAH62038.1.
PIRiJC7808.
RefSeqiNP_620276.1. NM_138921.1.
UniGeneiRn.76362.

3D structure databases

ProteinModelPortaliQ6P6T4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000046691.

PTM databases

iPTMnetiQ6P6T4.
PhosphoSitePlusiQ6P6T4.

Proteomic databases

PaxDbiQ6P6T4.
PRIDEiQ6P6T4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000041215; ENSRNOP00000046691; ENSRNOG00000030127. [Q6P6T4-2]
ENSRNOT00000078336; ENSRNOP00000075212; ENSRNOG00000030127. [Q6P6T4-1]
GeneIDi192360.
KEGGirno:192360.

Organism-specific databases

CTDi24139.
RGDi621066. Eml2.

Phylogenomic databases

eggNOGiKOG2106. Eukaryota.
ENOG410XNTQ. LUCA.
GeneTreeiENSGT00550000074369.
HOGENOMiHOG000294061.
HOVERGENiHBG051470.
InParanoidiQ6P6T4.
KOiK18595.
OMAiDDANDHI.
PhylomeDBiQ6P6T4.

Miscellaneous databases

PROiQ6P6T4.

Gene expression databases

BgeeiENSRNOG00000030127.
GenevisibleiQ6P6T4. RN.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR033016. EML2.
IPR005108. HELP.
IPR011041. Quinoprot_gluc/sorb_DH.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR13720:SF32. PTHR13720:SF32. 1 hit.
PfamiPF03451. HELP. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view]
SMARTiSM00320. WD40. 11 hits.
[Graphical view]
SUPFAMiSSF50952. SSF50952. 1 hit.
SSF50998. SSF50998. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEMAL2_RAT
AccessioniPrimary (citable) accession number: Q6P6T4
Secondary accession number(s): Q8VIM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.