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Protein

Echinoderm microtubule-associated protein-like 2

Gene

Eml2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Tubulin binding protein that inhibits microtubule nucleation and growth, resulting in shorter microtubules.By similarity

GO - Molecular functioni

  • catalytic activity Source: InterPro
  • microtubule binding Source: UniProtKB
  • protein C-terminus binding Source: RGD
  • receptor binding Source: RGD
  • tubulin binding Source: UniProtKB

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Echinoderm microtubule-associated protein-like 2
Short name:
EMAP-2
Gene namesi
Name:Eml2
Synonyms:Emap2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi621066 Eml2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002843891 – 649Echinoderm microtubule-associated protein-like 2Add BLAST649

Proteomic databases

PaxDbiQ6P6T4
PRIDEiQ6P6T4

PTM databases

iPTMnetiQ6P6T4
PhosphoSitePlusiQ6P6T4

Expressioni

Tissue specificityi

Widely expressed in both brain and peripheral tissues, including brainstem and enrichment in the postsynaptic density, PSD.1 Publication

Gene expression databases

BgeeiENSRNOG00000030127
GenevisibleiQ6P6T4 RN

Interactioni

Subunit structurei

Binds unpolymerized tubulins via its WD repeat region (By similarity). Interacts with GRID2 and may also interact with GRID1.By similarity1 Publication

GO - Molecular functioni

  • microtubule binding Source: UniProtKB
  • protein C-terminus binding Source: RGD
  • receptor binding Source: RGD
  • tubulin binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ6P6T4, 1 interactor
STRINGi10116.ENSRNOP00000046691

Structurei

3D structure databases

ProteinModelPortaliQ6P6T4
SMRiQ6P6T4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati56 – 93WD 1Add BLAST38
Repeati97 – 144WD 2Add BLAST48
Repeati151 – 192WD 3Add BLAST42
Repeati195 – 234WD 4Add BLAST40
Repeati241 – 280WD 5Add BLAST40
Repeati285 – 323WD 6Add BLAST39
Repeati369 – 406WD 7Add BLAST38
Repeati410 – 447WD 8Add BLAST38
Repeati452 – 489WD 9Add BLAST38
Repeati495 – 535WD 10Add BLAST41
Repeati564 – 602WD 11Add BLAST39
Repeati609 – 648WD 12Add BLAST40

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni10 – 649Tandem atypical propeller in EMLsBy similarityAdd BLAST640

Domaini

Contains a tandem atypical propeller in EMLs (TAPE) domain. The N-terminal beta-propeller is formed by canonical WD repeats; in contrast, the second beta-propeller contains one blade that is formed by discontinuous parts of the polypeptide chain (By similarity).By similarity

Sequence similaritiesi

Belongs to the WD repeat EMAP family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2106 Eukaryota
ENOG410XNTQ LUCA
GeneTreeiENSGT00550000074369
HOGENOMiHOG000294061
HOVERGENiHBG051470
InParanoidiQ6P6T4
KOiK18595
OMAiWATSTCT
PhylomeDBiQ6P6T4

Family and domain databases

Gene3Di2.130.10.10, 3 hits
InterProiView protein in InterPro
IPR005108 HELP
IPR011041 Quinoprot_gluc/sorb_DH
IPR011047 Quinoprotein_ADH-like_supfam
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
PfamiView protein in Pfam
PF03451 HELP, 1 hit
PF00400 WD40, 6 hits
SMARTiView protein in SMART
SM00320 WD40, 11 hits
SUPFAMiSSF50952 SSF50952, 1 hit
SSF50998 SSF50998, 1 hit
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 5 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6P6T4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSFGTGKTK EVIFSMEEGS VKMFLRGRPV PMLIPDELAP TYSLDTRSEL
60 70 80 90 100
PSSRLKLDWV YGYRGRDCRA NLYLLPTGEV VYFVASVAVL YSVEEQRQRH
110 120 130 140 150
YLGHNDDIKC LAVHPDMVTI ATGQVAGTTK EGKPLPPHVR VWDSVSLSTL
160 170 180 190 200
HVLGLGVFDR AVCCVAFSKS NGGNLLCAVD ESNDHVLSVW DWAKESKVVD
210 220 230 240 250
SKCSNEAVLV ATFHPTDPNL LITCGKSHIY FWSLEGGNLS KRQGLFEKHE
260 270 280 290 300
KPKYVLCVTF LEGGDVVTGD SGGNLYVWGK GGNRITQEVL GAHDGGVFAL
310 320 330 340 350
CALRDGTLVS GGGRDRRVVL WGSDYSKVQE VEVPEDFGPV RTVAEGRGDT
360 370 380 390 400
LYVGTTRNSI LLGSVHTGFS LLVQGHVEEL WGLATHPSRA QFVSCGQDKL
410 420 430 440 450
VHLWSSETHQ PVWSRSIEDP ARSAGFHPSG SVLAVGTVTG RWLLLDTDTR
460 470 480 490 500
DLVAIHTDGN EQISVVSFSP DGAYLAVGSH DNLVYVYTVD QGGRKVSRLG
510 520 530 540 550
KCSGHSSFIT HLDWAQDSTC FVTNSGDYEI LYWDAATCKQ ITSADTVRNV
560 570 580 590 600
QWATATCVLG FGVFGIWPEG ADGTDINAVA RSHDGNLLVS ADDFGKVHLF
610 620 630 640
SYPCCQPRAL SHKYGGHSSH VTNVAFLWDD SMVLTTGGKD TSVLQWRVA
Length:649
Mass (Da):70,711
Last modified:July 5, 2004 - v1
Checksum:iBCAEEA4699EEB9A9
GO
Isoform 2 (identifier: Q6P6T4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: Missing.

Note: No experimental confirmation available.
Show »
Length:634
Mass (Da):69,110
Checksum:i499137428649435D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti311 – 312GG → VW in AAL33537 (PubMed:11829466).Curated2
Sequence conflicti491 – 492QG → HC in AAL33537 (PubMed:11829466).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0244811 – 15Missing in isoform 2. 1 PublicationAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF335571 mRNA Translation: AAL33537.1
BC062038 mRNA Translation: AAH62038.1
PIRiJC7808
RefSeqiNP_620276.1, NM_138921.1
UniGeneiRn.76362

Genome annotation databases

EnsembliENSRNOT00000041215; ENSRNOP00000046691; ENSRNOG00000030127 [Q6P6T4-2]
ENSRNOT00000078336; ENSRNOP00000075212; ENSRNOG00000030127 [Q6P6T4-1]
GeneIDi192360
KEGGirno:192360

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiEMAL2_RAT
AccessioniPrimary (citable) accession number: Q6P6T4
Secondary accession number(s): Q8VIM8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: July 5, 2004
Last modified: March 28, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health