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Protein

Ectonucleoside triphosphate diphosphohydrolase 5

Gene

Entpd5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. The nucleotide hydrolyzing preference is GDP > IDP > UDP, but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling (By similarity).By similarity

Catalytic activityi

GDP + H2O = GMP + phosphate.
A nucleoside diphosphate + H2O = a nucleoside phosphate + phosphate.

Cofactori

Ca2+By similarity, Mg2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei171 – 1711Proton acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, Magnesium

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleoside triphosphate diphosphohydrolase 5 (EC:3.6.1.6)
Short name:
NTPDase 5
Alternative name(s):
Guanosine-diphosphatase ENTPD5 (EC:3.6.1.42)
Short name:
GDPase ENTPD5
Uridine-diphosphatase ENTPD5
Short name:
UDPase ENTPD5
Gene namesi
Name:Entpd5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi735219. Entpd5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 427403Ectonucleoside triphosphate diphosphohydrolase 5PRO_0000404542Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi231 – 2311N-linked (GlcNAc...)Sequence analysis
Disulfide bondi271 ↔ 302By similarity
Disulfide bondi362 ↔ 376By similarity

Post-translational modificationi

N-glycosylated; high-mannose type.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ6P6S9.
PRIDEiQ6P6S9.

Interactioni

Subunit structurei

Exists both as a monomer and a disulfide-linked homodimer, the dimers are enzymatically inactive.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000041026.

Structurei

3D structure databases

ProteinModelPortaliQ6P6S9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1385. Eukaryota.
COG5371. LUCA.
HOGENOMiHOG000220904.
HOVERGENiHBG018208.
InParanoidiQ6P6S9.
KOiK01511.
PhylomeDBiQ6P6S9.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6P6S9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATSWASVFL LIIAYVGGTV FYREQQTWFE GVFLSSMCPV NVSAGTFYGI
60 70 80 90 100
MFDAGSTGTR IHVYTFVQRT SGQLPFLEGE IFDSVKPGLS AFVDQPKQGA
110 120 130 140 150
ETVQELLEVA KDSIPRSHWK RTPVVLKATA GLRLLPEQKA QTLLLEVEEI
160 170 180 190 200
FKNSPFLVPD DSVSIMDGSY EGILAWVTVN FLTGQLHGRG QETVGTLDLG
210 220 230 240 250
GASTQITFLP QLEKTLEQTP KGYLASFEMF NSTFKLYTHS YLGFGLKAAR
260 270 280 290 300
LATLGALEAE GTDGHTFRSA CLPRWLEAEW IFGGVKYQYG GNQEGEMGFE
310 320 330 340 350
PCYAEVRRVV QGKLHQPEEI RGSSFYAFSY YYDRAAETHL IDYEKGGVLK
360 370 380 390 400
VEDFERKARE VCDNLESFSS GSPFLCMDLS YITALLKDGF GFEDGTLLQL
410 420
TKKVNNIETG WALGATFHLL QSLGITS
Length:427
Mass (Da):47,373
Last modified:July 5, 2004 - v1
Checksum:i6CBBC44D96A60211
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC062044 mRNA. Translation: AAH62044.1.
RefSeqiNP_955426.1. NM_199394.1.
UniGeneiRn.63533.

Genome annotation databases

GeneIDi314312.
KEGGirno:314312.
UCSCiRGD:735219. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC062044 mRNA. Translation: AAH62044.1.
RefSeqiNP_955426.1. NM_199394.1.
UniGeneiRn.63533.

3D structure databases

ProteinModelPortaliQ6P6S9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000041026.

Proteomic databases

PaxDbiQ6P6S9.
PRIDEiQ6P6S9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi314312.
KEGGirno:314312.
UCSCiRGD:735219. rat.

Organism-specific databases

CTDi957.
RGDi735219. Entpd5.

Phylogenomic databases

eggNOGiKOG1385. Eukaryota.
COG5371. LUCA.
HOGENOMiHOG000220904.
HOVERGENiHBG018208.
InParanoidiQ6P6S9.
KOiK01511.
PhylomeDBiQ6P6S9.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

NextBioi667451.
PROiQ6P6S9.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.

Entry informationi

Entry nameiENTP5_RAT
AccessioniPrimary (citable) accession number: Q6P6S9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: July 5, 2004
Last modified: December 9, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.