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Protein

O-phosphoseryl-tRNA(Sec) selenium transferase

Gene

Sepsecs

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis.By similarity

Catalytic activityi

O-phospho-L-seryl-tRNA(Sec) + selenophosphate + H2O = L-selenocysteinyl-tRNA(Sec) + 2 phosphate.By similarity

Cofactori

pyridoxal 5'-phosphate1 Publication

Pathwayi: selenocysteinyl-tRNA(Sec) biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route).By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. L-seryl-tRNA(Sec) kinase (Pstk)
  2. O-phosphoseryl-tRNA(Sec) selenium transferase (Sepsecs), O-phosphoseryl-tRNA(Sec) selenium transferase (Sepsecs)
This subpathway is part of the pathway selenocysteinyl-tRNA(Sec) biosynthesis, which is itself part of Aminoacyl-tRNA biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route), the pathway selenocysteinyl-tRNA(Sec) biosynthesis and in Aminoacyl-tRNA biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei74May act as a substrate filter by repelling compounds with a negatively charged alpha-carboxylate1 Publication1
Binding sitei75Pyridoxal phosphate1 Publication1
Binding sitei97Substrate1 Publication1
Binding sitei98Substrate1 Publication1
Binding sitei105Substrate1 Publication1
Binding sitei271tRNA variable arm1 Publication1
Binding sitei313Substrate1 Publication1
Binding sitei398tRNA discriminator base1 Publication1
Binding sitei463tRNA acceptor arm1 Publication1

GO - Molecular functioni

GO - Biological processi

  • selenocysteine incorporation Source: HGNC
  • selenocysteinyl-tRNA(Sec) biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

Pyridoxal phosphate, RNA-binding, Selenium, tRNA-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14964.
UniPathwayiUPA00906; UER00898.

Names & Taxonomyi

Protein namesi
Recommended name:
O-phosphoseryl-tRNA(Sec) selenium transferase (EC:2.9.1.2By similarity)
Alternative name(s):
Selenocysteine synthase
Short name:
Sec synthase
Selenocysteinyl-tRNA(Sec) synthase
Sep-tRNA:Sec-tRNA synthase
Short name:
SepSecS
UGA suppressor tRNA-associated protein
Gene namesi
Name:Sepsecs
Synonyms:D5Ertd135e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1098791. Sepsecs.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

  • cytoplasm Source: HGNC
  • nucleus Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi105Q → E: 50% of wild-type activity. 1 Publication1
Mutagenesisi313R → E: Virtually inactive. 1 Publication1
Mutagenesisi313R → S: 30% of wild-type activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002198761 – 504O-phosphoseryl-tRNA(Sec) selenium transferaseAdd BLAST504

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineBy similarity1
Modified residuei284N6-(pyridoxal phosphate)lysine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6P6M7.
MaxQBiQ6P6M7.
PaxDbiQ6P6M7.
PeptideAtlasiQ6P6M7.
PRIDEiQ6P6M7.

PTM databases

iPTMnetiQ6P6M7.
PhosphoSitePlusiQ6P6M7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000029173.
ExpressionAtlasiQ6P6M7. baseline and differential.
GenevisibleiQ6P6M7. MM.

Interactioni

Subunit structurei

Homotetramer formed by a catalytic dimer and a non-catalytic dimer serving as a binding platform that orients tRNASec for catalysis. Each tetramer binds the CCA ends of two tRNAs which point to the active sites of the catalytic dimer (By similarity).By similarity1 Publication

Protein-protein interaction databases

MINTiMINT-1857229.
STRINGi10090.ENSMUSP00000031069.

Structurei

Secondary structure

1504
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi24 – 38Combined sources15
Helixi48 – 60Combined sources13
Helixi63 – 65Combined sources3
Helixi82 – 87Combined sources6
Turni88 – 90Combined sources3
Beta strandi95 – 97Combined sources3
Helixi109 – 129Combined sources21
Beta strandi136 – 142Combined sources7
Helixi144 – 158Combined sources15
Beta strandi164 – 168Combined sources5
Helixi173 – 181Combined sources9
Beta strandi185 – 189Combined sources5
Beta strandi192 – 194Combined sources3
Beta strandi197 – 199Combined sources3
Helixi202 – 212Combined sources11
Helixi214 – 216Combined sources3
Beta strandi217 – 225Combined sources9
Helixi235 – 245Combined sources11
Beta strandi249 – 252Combined sources4
Turni254 – 258Combined sources5
Helixi260 – 272Combined sources13
Beta strandi277 – 281Combined sources5
Helixi282 – 286Combined sources5
Beta strandi293 – 298Combined sources6
Helixi300 – 309Combined sources10
Helixi317 – 358Combined sources42
Beta strandi370 – 376Combined sources7
Turni378 – 380Combined sources3
Beta strandi381 – 385Combined sources5
Helixi387 – 397Combined sources11
Beta strandi404 – 406Combined sources3
Beta strandi411 – 414Combined sources4
Beta strandi417 – 420Combined sources4
Turni421 – 425Combined sources5
Beta strandi433 – 437Combined sources5
Helixi444 – 465Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BC8X-ray1.65A19-468[»]
3BCAX-ray2.25A19-468[»]
3BCBX-ray1.85A19-468[»]
ProteinModelPortaliQ6P6M7.
SMRiQ6P6M7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6P6M7.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 44Tetramerization1 PublicationAdd BLAST44
Regioni96 – 106Phosphate loop (P-loop)1 PublicationAdd BLAST11

Sequence similaritiesi

Belongs to the SepSecS family.Curated

Phylogenomic databases

eggNOGiKOG3843. Eukaryota.
COG0076. LUCA.
GeneTreeiENSGT00390000007332.
HOGENOMiHOG000254245.
HOVERGENiHBG061363.
InParanoidiQ6P6M7.
KOiK03341.
OMAiPCPYLNA.
OrthoDBiEOG091G071Q.
PhylomeDBiQ6P6M7.
TreeFamiTF314381.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR019872. Sec-tRNA_Se_transferase.
IPR008829. SepSecS/SepCysS.
[Graphical view]
PANTHERiPTHR12944. PTHR12944. 1 hit.
PfamiPF05889. SepSecS. 1 hit.
[Graphical view]
PIRSFiPIRSF017689. SepSecS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR03531. selenium_SpcS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6P6M7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPESFAAGE RRVSPAYVRQ GCEARRAHEH LIRLLLEQGK CPEDGWDEST
60 70 80 90 100
LELFLHELAV MDSNNFLGNC GVGEREGRVA SALVARRHYR FIHGIGRSGD
110 120 130 140 150
ISAVQPKAAG SSLLNKITNS LVLNVIKLAG VHSVASCFVV PMATGMSLTL
160 170 180 190 200
CFLTLRHKRP KAKYIIWPRI DQKSCFKSMV TAGFEPVVIE NVLEGDELRT
210 220 230 240 250
DLKAVEAKIQ ELGPEHILCL HSTTACFAPR VPDRLEELAV ICANYDIPHV
260 270 280 290 300
VNNAYGLQSS KCMHLIQQGA RVGRIDAFVQ SLDKNFMVPV GGAIIAGFNE
310 320 330 340 350
PFIQDISKMY PGRASASPSL DVLITLLSLG CSGYRKLLKE RKEMFVYLST
360 370 380 390 400
QLKKLAEAHN ERLLQTPHNP ISLAMTLKTI DGHHDKAVTQ LGSMLFTRQV
410 420 430 440 450
SGARAVPLGN VQTVSGHTFR GFMSHADNYP CAYLNAAAAI GMKMQDVDLF
460 470 480 490 500
IKRLDKCLNI VRKEQTRASV VSGADRNKAE DADIEEMALK LDDVLGDVGQ

GPAL
Length:504
Mass (Da):55,326
Last modified:March 29, 2005 - v2
Checksum:iE106674A0C80D98B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti300E → D in AAH62133 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033654 mRNA. Translation: BAC28410.1.
BC062133 mRNA. Translation: AAH62133.1.
CCDSiCCDS19286.1.
RefSeqiNP_766078.1. NM_172490.3.
UniGeneiMm.244719.

Genome annotation databases

EnsembliENSMUST00000031069; ENSMUSP00000031069; ENSMUSG00000029173.
GeneIDi211006.
KEGGimmu:211006.
UCSCiuc008xko.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033654 mRNA. Translation: BAC28410.1.
BC062133 mRNA. Translation: AAH62133.1.
CCDSiCCDS19286.1.
RefSeqiNP_766078.1. NM_172490.3.
UniGeneiMm.244719.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BC8X-ray1.65A19-468[»]
3BCAX-ray2.25A19-468[»]
3BCBX-ray1.85A19-468[»]
ProteinModelPortaliQ6P6M7.
SMRiQ6P6M7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1857229.
STRINGi10090.ENSMUSP00000031069.

PTM databases

iPTMnetiQ6P6M7.
PhosphoSitePlusiQ6P6M7.

Proteomic databases

EPDiQ6P6M7.
MaxQBiQ6P6M7.
PaxDbiQ6P6M7.
PeptideAtlasiQ6P6M7.
PRIDEiQ6P6M7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031069; ENSMUSP00000031069; ENSMUSG00000029173.
GeneIDi211006.
KEGGimmu:211006.
UCSCiuc008xko.1. mouse.

Organism-specific databases

CTDi51091.
MGIiMGI:1098791. Sepsecs.

Phylogenomic databases

eggNOGiKOG3843. Eukaryota.
COG0076. LUCA.
GeneTreeiENSGT00390000007332.
HOGENOMiHOG000254245.
HOVERGENiHBG061363.
InParanoidiQ6P6M7.
KOiK03341.
OMAiPCPYLNA.
OrthoDBiEOG091G071Q.
PhylomeDBiQ6P6M7.
TreeFamiTF314381.

Enzyme and pathway databases

UniPathwayiUPA00906; UER00898.
BioCyciMetaCyc:MONOMER-14964.

Miscellaneous databases

EvolutionaryTraceiQ6P6M7.
PROiQ6P6M7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029173.
ExpressionAtlasiQ6P6M7. baseline and differential.
GenevisibleiQ6P6M7. MM.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR019872. Sec-tRNA_Se_transferase.
IPR008829. SepSecS/SepCysS.
[Graphical view]
PANTHERiPTHR12944. PTHR12944. 1 hit.
PfamiPF05889. SepSecS. 1 hit.
[Graphical view]
PIRSFiPIRSF017689. SepSecS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR03531. selenium_SpcS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSPCS_MOUSE
AccessioniPrimary (citable) accession number: Q6P6M7
Secondary accession number(s): Q8BZS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: November 2, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.