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Q6P6M7

- SPCS_MOUSE

UniProt

Q6P6M7 - SPCS_MOUSE

Protein

O-phosphoseryl-tRNA(Sec) selenium transferase

Gene

Sepsecs

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 2 (29 Mar 2005)
      Previous versions | rss
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    Functioni

    Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis.By similarity

    Catalytic activityi

    O-phospho-L-seryl-tRNA(Sec) + selenophosphate + H2O = L-selenocysteinyl-tRNA(Sec) + 2 phosphate.

    Cofactori

    Pyridoxal phosphate.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei74 – 741May act as a substrate filter by repelling compounds with a negatively charged alpha-carboxylate
    Binding sitei75 – 751PLP
    Binding sitei97 – 971Substrate
    Binding sitei98 – 981Substrate
    Binding sitei105 – 1051Substrate
    Binding sitei271 – 2711tRNA variable armBy similarity
    Binding sitei313 – 3131Substrate
    Binding sitei398 – 3981tRNA discriminator baseBy similarity

    GO - Molecular functioni

    1. protein binding Source: HGNC
    2. pyridoxal phosphate binding Source: InterPro
    3. transferase activity, transferring selenium-containing groups Source: InterPro
    4. tRNA binding Source: HGNC

    GO - Biological processi

    1. selenocysteine incorporation Source: HGNC
    2. selenocysteinyl-tRNA(Sec) biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate, RNA-binding, Selenium, tRNA-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14964.
    UniPathwayiUPA00906; UER00898.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    O-phosphoseryl-tRNA(Sec) selenium transferase (EC:2.9.1.2)
    Alternative name(s):
    Selenocysteine synthase
    Short name:
    Sec synthase
    Selenocysteinyl-tRNA(Sec) synthase
    Sep-tRNA:Sec-tRNA synthase
    Short name:
    SepSecS
    UGA suppressor tRNA-associated protein
    Gene namesi
    Name:Sepsecs
    Synonyms:D5Ertd135e
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1098791. Sepsecs.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: HGNC
    2. nucleus Source: HGNC

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi105 – 1051Q → E: 50% of wild-type activity. 1 Publication
    Mutagenesisi313 – 3131R → E: Virtually inactive. 1 Publication
    Mutagenesisi313 – 3131R → S: 30% of wild-type activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 504504O-phosphoseryl-tRNA(Sec) selenium transferasePRO_0000219876Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei284 – 2841N6-(pyridoxal phosphate)lysine

    Proteomic databases

    MaxQBiQ6P6M7.
    PaxDbiQ6P6M7.
    PRIDEiQ6P6M7.

    PTM databases

    PhosphoSiteiQ6P6M7.

    Expressioni

    Gene expression databases

    ArrayExpressiQ6P6M7.
    BgeeiQ6P6M7.
    GenevestigatoriQ6P6M7.

    Interactioni

    Subunit structurei

    Homotetramer formed by a catalytic dimer and a non-catalytic dimer serving as a binding platform that orients tRNASec for catalysis.1 Publication

    Protein-protein interaction databases

    MINTiMINT-1857229.
    STRINGi10090.ENSMUSP00000031069.

    Structurei

    Secondary structure

    1
    504
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi24 – 3815
    Helixi48 – 6013
    Helixi63 – 653
    Helixi82 – 876
    Turni88 – 903
    Beta strandi95 – 973
    Helixi109 – 12921
    Beta strandi136 – 1427
    Helixi144 – 15815
    Beta strandi164 – 1685
    Helixi173 – 1819
    Beta strandi185 – 1895
    Beta strandi192 – 1943
    Beta strandi197 – 1993
    Helixi202 – 21211
    Helixi214 – 2163
    Beta strandi217 – 2259
    Helixi235 – 24511
    Beta strandi249 – 2524
    Turni254 – 2585
    Helixi260 – 27213
    Beta strandi277 – 2815
    Helixi282 – 2865
    Beta strandi293 – 2986
    Helixi300 – 30910
    Helixi317 – 35842
    Beta strandi370 – 3767
    Turni378 – 3803
    Beta strandi381 – 3855
    Helixi387 – 39711
    Beta strandi404 – 4063
    Beta strandi411 – 4144
    Beta strandi417 – 4204
    Turni421 – 4255
    Beta strandi433 – 4375
    Helixi444 – 46522

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3BC8X-ray1.65A19-468[»]
    3BCAX-ray2.25A19-468[»]
    3BCBX-ray1.85A19-468[»]
    ProteinModelPortaliQ6P6M7.
    SMRiQ6P6M7. Positions 23-467.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ6P6M7.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 4444TetramerizationAdd
    BLAST
    Regioni96 – 10611Phosphate loop (P-loop)Add
    BLAST

    Sequence similaritiesi

    Belongs to the SepSecS family.Curated

    Phylogenomic databases

    eggNOGiCOG0076.
    GeneTreeiENSGT00390000007332.
    HOGENOMiHOG000254245.
    HOVERGENiHBG061363.
    InParanoidiQ6P6M7.
    KOiK03341.
    OMAiNDYPCAY.
    OrthoDBiEOG71G9TX.
    PhylomeDBiQ6P6M7.
    TreeFamiTF314381.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    InterProiIPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR019872. Sec-tRNA_Se_transferase.
    IPR008829. SLA/LP_auto_ag.
    [Graphical view]
    PANTHERiPTHR12944. PTHR12944. 1 hit.
    PfamiPF05889. SLA_LP_auto_ag. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017689. SepSecS. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR03531. selenium_SpcS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q6P6M7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNPESFAAGE RRVSPAYVRQ GCEARRAHEH LIRLLLEQGK CPEDGWDEST    50
    LELFLHELAV MDSNNFLGNC GVGEREGRVA SALVARRHYR FIHGIGRSGD 100
    ISAVQPKAAG SSLLNKITNS LVLNVIKLAG VHSVASCFVV PMATGMSLTL 150
    CFLTLRHKRP KAKYIIWPRI DQKSCFKSMV TAGFEPVVIE NVLEGDELRT 200
    DLKAVEAKIQ ELGPEHILCL HSTTACFAPR VPDRLEELAV ICANYDIPHV 250
    VNNAYGLQSS KCMHLIQQGA RVGRIDAFVQ SLDKNFMVPV GGAIIAGFNE 300
    PFIQDISKMY PGRASASPSL DVLITLLSLG CSGYRKLLKE RKEMFVYLST 350
    QLKKLAEAHN ERLLQTPHNP ISLAMTLKTI DGHHDKAVTQ LGSMLFTRQV 400
    SGARAVPLGN VQTVSGHTFR GFMSHADNYP CAYLNAAAAI GMKMQDVDLF 450
    IKRLDKCLNI VRKEQTRASV VSGADRNKAE DADIEEMALK LDDVLGDVGQ 500
    GPAL 504
    Length:504
    Mass (Da):55,326
    Last modified:March 29, 2005 - v2
    Checksum:iE106674A0C80D98B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti300 – 3001E → D in AAH62133. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK033654 mRNA. Translation: BAC28410.1.
    BC062133 mRNA. Translation: AAH62133.1.
    CCDSiCCDS19286.1.
    RefSeqiNP_766078.1. NM_172490.3.
    UniGeneiMm.244719.

    Genome annotation databases

    EnsembliENSMUST00000031069; ENSMUSP00000031069; ENSMUSG00000029173.
    GeneIDi211006.
    KEGGimmu:211006.
    UCSCiuc008xko.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK033654 mRNA. Translation: BAC28410.1 .
    BC062133 mRNA. Translation: AAH62133.1 .
    CCDSi CCDS19286.1.
    RefSeqi NP_766078.1. NM_172490.3.
    UniGenei Mm.244719.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3BC8 X-ray 1.65 A 19-468 [» ]
    3BCA X-ray 2.25 A 19-468 [» ]
    3BCB X-ray 1.85 A 19-468 [» ]
    ProteinModelPortali Q6P6M7.
    SMRi Q6P6M7. Positions 23-467.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-1857229.
    STRINGi 10090.ENSMUSP00000031069.

    PTM databases

    PhosphoSitei Q6P6M7.

    Proteomic databases

    MaxQBi Q6P6M7.
    PaxDbi Q6P6M7.
    PRIDEi Q6P6M7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000031069 ; ENSMUSP00000031069 ; ENSMUSG00000029173 .
    GeneIDi 211006.
    KEGGi mmu:211006.
    UCSCi uc008xko.1. mouse.

    Organism-specific databases

    CTDi 51091.
    MGIi MGI:1098791. Sepsecs.

    Phylogenomic databases

    eggNOGi COG0076.
    GeneTreei ENSGT00390000007332.
    HOGENOMi HOG000254245.
    HOVERGENi HBG061363.
    InParanoidi Q6P6M7.
    KOi K03341.
    OMAi NDYPCAY.
    OrthoDBi EOG71G9TX.
    PhylomeDBi Q6P6M7.
    TreeFami TF314381.

    Enzyme and pathway databases

    UniPathwayi UPA00906 ; UER00898 .
    BioCyci MetaCyc:MONOMER-14964.

    Miscellaneous databases

    EvolutionaryTracei Q6P6M7.
    NextBioi 373121.
    PROi Q6P6M7.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q6P6M7.
    Bgeei Q6P6M7.
    Genevestigatori Q6P6M7.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    InterProi IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR019872. Sec-tRNA_Se_transferase.
    IPR008829. SLA/LP_auto_ag.
    [Graphical view ]
    PANTHERi PTHR12944. PTHR12944. 1 hit.
    Pfami PF05889. SLA_LP_auto_ag. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017689. SepSecS. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR03531. selenium_SpcS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Cecum.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Limb.
    3. "Structure and catalytic mechanism of eukaryotic selenocysteine synthase."
      Ganichkin O.M., Xu X.M., Carlson B.A., Mix H., Hatfield D.L., Gladyshev V.N., Wahl M.C.
      J. Biol. Chem. 283:5849-5865(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 19-468 IN COMPLEX WITH PYRIDOXAL PHOSPHATE, SUBUNIT, SUBSTRATE-BINDING SITES, COFACTOR, MUTAGENESIS OF GLN-105 AND ARG-313.

    Entry informationi

    Entry nameiSPCS_MOUSE
    AccessioniPrimary (citable) accession number: Q6P6M7
    Secondary accession number(s): Q8BZS6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: March 29, 2005
    Last modified: October 1, 2014
    This is version 87 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3