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Protein

Plastin-2

Gene

lcp1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Actin-binding protein. Plays a role in the activation of T-cells (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi22 – 33121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi62 – 73122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Plastin-2
Alternative name(s):
L-plastin
Lymphocyte cytosolic plastin 1
Gene namesi
Name:lcp1Imported
Synonyms:pls2
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-991213-5. lcp1.

Subcellular locationi

  • Cytoplasmcytoskeleton By similarity
  • Cell junction By similarity
  • Cell projection By similarity
  • Cell projectionruffle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity

GO - Cellular componenti

  • cell junction Source: UniProtKB-SubCell
  • cytoplasm Source: ZFIN
  • cytoskeleton Source: UniProtKB-SubCell
  • ruffle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 624624Plastin-2PRO_0000073745Add
BLAST

Proteomic databases

PaxDbiQ6P698.
PRIDEiQ6P698.

Expressioni

Developmental stagei

Expressed in macrophage precursors as they migrate from the midline to the yolksac during somatogenesis. Prior to blood circulation, continues to be expressed in maturing macrophage precursors in the yolksac and in those that have migrated to the mesenchyme of the head. At 28 hours post-fertilization (hpf), also expressed in the caudal part of the axial vein and the surrounding mesenchyme.1 Publication

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000108316.

Structurei

3D structure databases

ProteinModelPortaliQ6P698.
SMRiQ6P698. Positions 116-370, 516-624.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 4436EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini49 – 8436EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini118 – 373256Actin-binding 1Sequence analysisAdd
BLAST
Domaini118 – 234117CH 1PROSITE-ProRule annotationAdd
BLAST
Domaini262 – 373112CH 2PROSITE-ProRule annotationAdd
BLAST
Domaini392 – 621230Actin-binding 2Sequence analysisAdd
BLAST
Domaini392 – 501110CH 3PROSITE-ProRule annotationAdd
BLAST
Domaini513 – 621109CH 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 actin-binding domains.Sequence analysis
Contains 4 CH (calponin-homology) domains.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0046. Eukaryota.
COG5069. LUCA.
HOGENOMiHOG000213447.
HOVERGENiHBG003082.
InParanoidiQ6P698.
KOiK17276.
PhylomeDBiQ6P698.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.418.10. 4 hits.
InterProiIPR001589. Actinin_actin-bd_CS.
IPR001715. CH-domain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF00307. CH. 4 hits.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00033. CH. 4 hits.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS00019. ACTININ_1. 2 hits.
PS00020. ACTININ_2. 2 hits.
PS50021. CH. 4 hits.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6P698-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAQISAEE MEELREAFTK VDVDGNGHIS TDELNALFKA ANLPLPGYRV
60 70 80 90 100
REIIQEISRT MDLNQDGKIT FDEFAKVVHD LKSSEVAKTF RKAINKKEGI
110 120 130 140 150
CSVAGTSEQS GTQHSYSEEE KVAFVNWVNK ALEKDPDCQH VLPMDPSTDD
160 170 180 190 200
LFTAVGDGIV LCKMINLSVP DTIDERTINK KKLTPFTIQE NLNLALNSAS
210 220 230 240 250
AIGCHVVNIG AEDLKEGRQH LVLGLLWQVI KIGLFADIEI SRNEALIALL
260 270 280 290 300
RDGESLEDLV KLSPEELLLR WANYHLEEAG CPKINNFSSD IKDSKAYYNI
310 320 330 340 350
LNQVAPKRDE EGIPAIPIDI SGIREKDDLK RAECMLEQAD RLGCRQFVTA
360 370 380 390 400
TDVVRGNPKL NLAYVANLFN KYPALKKPEN QDIDWSSIEG ETREERTFRN
410 420 430 440 450
WMNSLGVNPR VNHLYVDLAD ALVIFQLYEK IKVPVDWDKV NKPPYPKLGS
460 470 480 490 500
NMKKLENCNY AVELGKKEAK FSLVGIAGQD LNEGNRTLTL ALLWQLMRRY
510 520 530 540 550
TLNILEDLGD GQKIIDETIV QWVNETLTQA GKGTISGFKD GSISSSMPVL
560 570 580 590 600
DLIDAIQPGS IRYDLLKAED LTDEKKLNNA KYAISMARKI GARVYALPED
610 620
LVEVKPKMVM TVFACLMARG MRRI
Length:624
Mass (Da):69,900
Last modified:July 5, 2004 - v1
Checksum:iF78D17B8BF1D4D6F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti423 – 4242VI → HE in AAD40680 (PubMed:10433904).Curated
Sequence conflicti438 – 4381D → G in AAD40680 (PubMed:10433904).Curated
Sequence conflicti575 – 5751K → E in AAD40680 (PubMed:10433904).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC062381 mRNA. Translation: AAH62381.1.
AF157110 mRNA. Translation: AAD40680.1.
RefSeqiNP_571395.2. NM_131320.2.
UniGeneiDr.25823.

Genome annotation databases

GeneIDi30583.
KEGGidre:30583.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC062381 mRNA. Translation: AAH62381.1.
AF157110 mRNA. Translation: AAD40680.1.
RefSeqiNP_571395.2. NM_131320.2.
UniGeneiDr.25823.

3D structure databases

ProteinModelPortaliQ6P698.
SMRiQ6P698. Positions 116-370, 516-624.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000108316.

Proteomic databases

PaxDbiQ6P698.
PRIDEiQ6P698.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi30583.
KEGGidre:30583.

Organism-specific databases

CTDi3936.
ZFINiZDB-GENE-991213-5. lcp1.

Phylogenomic databases

eggNOGiKOG0046. Eukaryota.
COG5069. LUCA.
HOGENOMiHOG000213447.
HOVERGENiHBG003082.
InParanoidiQ6P698.
KOiK17276.
PhylomeDBiQ6P698.

Miscellaneous databases

NextBioi20806953.
PROiQ6P698.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.418.10. 4 hits.
InterProiIPR001589. Actinin_actin-bd_CS.
IPR001715. CH-domain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF00307. CH. 4 hits.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00033. CH. 4 hits.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS00019. ACTININ_1. 2 hits.
PS00020. ACTININ_2. 2 hits.
PS50021. CH. 4 hits.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: KidneyImported.
  2. "Ontogeny and behaviour of early macrophages in the zebrafish embryo."
    Herbomel P., Thisse B., Thisse C.
    Development 126:3735-3745(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 423-624, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiPLSL_DANRE
AccessioniPrimary (citable) accession number: Q6P698
Secondary accession number(s): Q9W746
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: July 5, 2004
Last modified: March 16, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.