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Protein

Serine/threonine-protein kinase N3

Gene

PKN3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Contributes to invasiveness in malignant prostate cancer.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Two specific sites, Thr-718 (activation loop of the kinase domain) and Thr-860 (turn motif), need to be phosphorylated for its full activation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei588ATPPROSITE-ProRule annotation1
Active sitei684Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi565 – 573ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • epithelial cell migration Source: UniProtKB
  • intracellular signal transduction Source: GO_Central
  • peptidyl-serine phosphorylation Source: GO_Central
  • protein phosphorylation Source: ProtInc
  • signal transduction Source: ProtInc

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-5625740 RHO GTPases activate PKNs
SignaLinkiQ6P5Z2

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase N3 (EC:2.7.11.13)
Alternative name(s):
Protein kinase PKN-beta
Protein-kinase C-related kinase 3
Gene namesi
Name:PKN3
Synonyms:PKNBETA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000160447.6
HGNCiHGNC:17999 PKN3
MIMi610714 gene
neXtProtiNX_Q6P5Z2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi588K → E: Abolishes autophosphorylation and catalytic activity. 2 Publications1
Mutagenesisi588K → R: Abolishes catalytic activity. 2 Publications1
Mutagenesisi718T → A: Abolishes phosphorylation. 1 Publication1

Organism-specific databases

DisGeNETi29941
OpenTargetsiENSG00000160447
PharmGKBiPA134919098

Chemistry databases

ChEMBLiCHEMBL3627581

Polymorphism and mutation databases

BioMutaiPKN3
DMDMi74749130

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000557251 – 889Serine/threonine-protein kinase N3Add BLAST889

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei171PhosphoserineCombined sources1
Modified residuei544PhosphoserineCombined sources1
Modified residuei548PhosphoserineCombined sources1
Modified residuei718Phosphothreonine1 Publication1
Modified residuei722PhosphothreonineCombined sources1
Modified residuei860PhosphothreonineCombined sources1

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6P5Z2
MaxQBiQ6P5Z2
PaxDbiQ6P5Z2
PeptideAtlasiQ6P5Z2
PRIDEiQ6P5Z2

PTM databases

iPTMnetiQ6P5Z2
PhosphoSitePlusiQ6P5Z2

Expressioni

Tissue specificityi

Expressed in prostate tumors and various cancer cell lines. Not expressed in adult tissues.1 Publication

Gene expression databases

BgeeiENSG00000160447
CleanExiHS_PKN3
ExpressionAtlasiQ6P5Z2 baseline and differential
GenevisibleiQ6P5Z2 HS

Organism-specific databases

HPAiHPA045390
HPA058305

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi118978, 94 interactors
IntActiQ6P5Z2, 38 interactors
MINTiQ6P5Z2
STRINGi9606.ENSP00000291906

Chemistry databases

BindingDBiQ6P5Z2

Structurei

3D structure databases

ProteinModelPortaliQ6P5Z2
SMRiQ6P5Z2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 80REM-1 1PROSITE-ProRule annotationAdd BLAST76
Domaini93 – 163REM-1 2PROSITE-ProRule annotationAdd BLAST71
Domaini165 – 245REM-1 3PROSITE-ProRule annotationAdd BLAST81
Domaini559 – 818Protein kinasePROSITE-ProRule annotationAdd BLAST260
Domaini819 – 889AGC-kinase C-terminalAdd BLAST71

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi463 – 554Pro-richAdd BLAST92

Domaini

The C1 domain does not bind the diacylglycerol (DAG).

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0694 Eukaryota
ENOG410XNPH LUCA
GeneTreeiENSGT00820000126964
HOGENOMiHOG000233032
HOVERGENiHBG108317
InParanoidiQ6P5Z2
KOiK06071
OMAiCPEKRLG
OrthoDBiEOG091G00YT
PhylomeDBiQ6P5Z2
TreeFamiTF102005

Family and domain databases

CDDicd11622 HR1_PKN_1, 1 hit
InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR011072 HR1_rho-bd
IPR036274 HR1_rpt_sf
IPR011009 Kinase-like_dom_sf
IPR017892 Pkinase_C
IPR037313 PKN_HR1_1
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF02185 HR1, 3 hits
PF00069 Pkinase, 1 hit
PF00433 Pkinase_C, 1 hit
SMARTiView protein in SMART
SM00742 Hr1, 3 hits
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF46585 SSF46585, 3 hits
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS51860 REM_1, 3 hits

Sequencei

Sequence statusi: Complete.

Q6P5Z2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEGAPRQPG PSQWPPEDEK EVIRRAIQKE LKIKEGVENL RRVATDRRHL
60 70 80 90 100
GHVQQLLRSS NRRLEQLHGE LRELHARILL PGPGPGPAEP VASGPRPWAE
110 120 130 140 150
QLRARHLEAL RRQLHVELKV KQGAENMTHT CASGTPKERK LLAAAQQMLR
160 170 180 190 200
DSQLKVALLR MKISSLEASG SPEPGPELLA EELQHRLHVE AAVAEGAKNV
210 220 230 240 250
VKLLSSRRTQ DRKALAEAQA QLQESSQKLD LLRLALEQLL EQLPPAHPLR
260 270 280 290 300
SRVTRELRAA VPGYPQPSGT PVKPTALTGT LQVRLLGCEQ LLTAVPGRSP
310 320 330 340 350
AAALASSPSE GWLRTKAKHQ RGRGELASEV LAVLKVDNRV VGQTGWGQVA
360 370 380 390 400
EQSWDQTFVI PLERARELEI GVHWRDWRQL CGVAFLRLED FLDNACHQLS
410 420 430 440 450
LSLVPQGLLF AQVTFCDPVI ERRPRLQRQE RIFSKRRGQD FLRASQMNLG
460 470 480 490 500
MAAWGRLVMN LLPPCSSPST ISPPKGCPRT PTTLREASDP ATPSNFLPKK
510 520 530 540 550
TPLGEEMTPP PKPPRLYLPQ EPTSEETPRT KRPHMEPRTR RGPSPPASPT
560 570 580 590 600
RKPPRLQDFR CLAVLGRGHF GKVLLVQFKG TGKYYAIKAL KKQEVLSRDE
610 620 630 640 650
IESLYCEKRI LEAVGCTGHP FLLSLLACFQ TSSHACFVTE FVPGGDLMMQ
660 670 680 690 700
IHEDVFPEPQ ARFYVACVVL GLQFLHEKKI IYRDLKLDNL LLDAQGFLKI
710 720 730 740 750
ADFGLCKEGI GFGDRTSTFC GTPEFLAPEV LTQEAYTRAV DWWGLGVLLY
760 770 780 790 800
EMLVGECPFP GDTEEEVFDC IVNMDAPYPG FLSVQGLEFI QKLLQKCPEK
810 820 830 840 850
RLGAGEQDAE EIKVQPFFRT TNWQALLART IQPPFVPTLC GPADLRYFEG
860 870 880
EFTGLPPALT PPAPHSLLTA RQQAAFRDFD FVSERFLEP
Length:889
Mass (Da):99,421
Last modified:July 5, 2004 - v1
Checksum:iF3891DA1420DF54E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti444A → R in BAA85625 (PubMed:10441506).Curated1
Sequence conflicti627A → V in BAA85625 (PubMed:10441506).Curated1
Sequence conflicti636C → R in BAA85625 (PubMed:10441506).Curated1
Sequence conflicti738R → Q in BAA85625 (PubMed:10441506).Curated1
Sequence conflicti744G → A in BAA85625 (PubMed:10441506).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_042346180A → E1 PublicationCorresponds to variant dbSNP:rs56251280Ensembl.1
Natural variantiVAR_050565404V → L. Corresponds to variant dbSNP:rs12932Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019692 mRNA Translation: BAA85625.1
AL441992 Genomic DNA No translation available.
BC062558 mRNA Translation: AAH62558.1
CCDSiCCDS6908.1
PIRiJC7083
RefSeqiNP_037487.2, NM_013355.4
UniGeneiHs.300485
Hs.674749

Genome annotation databases

EnsembliENST00000291906; ENSP00000291906; ENSG00000160447
GeneIDi29941
KEGGihsa:29941
UCSCiuc004bvw.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPKN3_HUMAN
AccessioniPrimary (citable) accession number: Q6P5Z2
Secondary accession number(s): Q9UM03
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families
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