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Q6P5Z2

- PKN3_HUMAN

UniProt

Q6P5Z2 - PKN3_HUMAN

Protein

Serine/threonine-protein kinase N3

Gene

PKN3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Contributes to invasiveness in malignant prostate cancer.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Two specific sites, Thr-718 (activation loop of the kinase domain) and Thr-860 (turn motif), need to be phosphorylated for its full activation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei588 – 5881ATPPROSITE-ProRule annotation
    Active sitei684 – 6841Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi565 – 5739ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein kinase activity Source: ProtInc
    4. protein kinase C activity Source: UniProtKB-EC

    GO - Biological processi

    1. epithelial cell migration Source: UniProtKB
    2. protein phosphorylation Source: ProtInc
    3. signal transduction Source: ProtInc

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    SignaLinkiQ6P5Z2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase N3 (EC:2.7.11.13)
    Alternative name(s):
    Protein kinase PKN-beta
    Protein-kinase C-related kinase 3
    Gene namesi
    Name:PKN3
    Synonyms:PKNBETA
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 9

    Organism-specific databases

    HGNCiHGNC:17999. PKN3.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasmperinuclear region 1 Publication
    Note: Nuclear and perinuclear Golgi region.

    GO - Cellular componenti

    1. Golgi apparatus Source: ProtInc
    2. nucleus Source: ProtInc
    3. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi588 – 5881K → E: Abolishes autophosphorylation and catalytic activity. 2 Publications
    Mutagenesisi588 – 5881K → R: Abolishes catalytic activity. 2 Publications
    Mutagenesisi718 – 7181T → A: Abolishes phosphorylation. 1 Publication

    Organism-specific databases

    PharmGKBiPA134919098.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 889889Serine/threonine-protein kinase N3PRO_0000055725Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei544 – 5441Phosphoserine3 Publications
    Modified residuei548 – 5481Phosphoserine1 Publication
    Modified residuei718 – 7181Phosphothreonine1 Publication
    Modified residuei722 – 7221Phosphothreonine1 Publication
    Modified residuei860 – 8601Phosphothreonine2 Publications

    Post-translational modificationi

    Autophosphorylated.4 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ6P5Z2.
    PaxDbiQ6P5Z2.
    PRIDEiQ6P5Z2.

    PTM databases

    PhosphoSiteiQ6P5Z2.

    Expressioni

    Tissue specificityi

    Expressed in prostate tumors and various cancer cell lines. Not expressed in adult tissues.1 Publication

    Gene expression databases

    ArrayExpressiQ6P5Z2.
    BgeeiQ6P5Z2.
    CleanExiHS_PKN3.
    GenevestigatoriQ6P5Z2.

    Organism-specific databases

    HPAiHPA045390.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ARHGAP10A1A4S64EBI-1384335,EBI-1390944
    ARHGAP26Q9UNA14EBI-1384335,EBI-1390913

    Protein-protein interaction databases

    BioGridi118978. 5 interactions.
    IntActiQ6P5Z2. 3 interactions.
    MINTiMINT-192027.
    STRINGi9606.ENSP00000291906.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6P5Z2.
    SMRiQ6P5Z2. Positions 27-78, 109-163, 183-241, 525-884.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati18 – 9073REM 1Add
    BLAST
    Repeati105 – 18177REM 2Add
    BLAST
    Repeati182 – 25574REM 3Add
    BLAST
    Domaini559 – 818260Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini819 – 88971AGC-kinase C-terminalAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi463 – 55492Pro-richAdd
    BLAST

    Domaini

    The C1 domain does not bind the diacylglycerol (DAG).

    Sequence similaritiesi

    Contains 1 AGC-kinase C-terminal domain.Curated
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 3 REM (Hr1) repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233032.
    HOVERGENiHBG108317.
    InParanoidiQ6P5Z2.
    KOiK06071.
    OMAiQPFFRTT.
    OrthoDBiEOG7X9G6Q.
    PhylomeDBiQ6P5Z2.
    TreeFamiTF102005.

    Family and domain databases

    Gene3Di1.10.287.160. 3 hits.
    2.60.40.150. 1 hit.
    InterProiIPR000961. AGC-kinase_C.
    IPR000008. C2_dom.
    IPR011072. HR1_rho-bd.
    IPR011009. Kinase-like_dom.
    IPR017892. Pkinase_C.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF02185. HR1. 3 hits.
    PF00069. Pkinase. 1 hit.
    PF00433. Pkinase_C. 1 hit.
    [Graphical view]
    SMARTiSM00239. C2. 1 hit.
    SM00742. Hr1. 3 hits.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF46585. SSF46585. 3 hits.
    SSF49562. SSF49562. 2 hits.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6P5Z2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEEGAPRQPG PSQWPPEDEK EVIRRAIQKE LKIKEGVENL RRVATDRRHL    50
    GHVQQLLRSS NRRLEQLHGE LRELHARILL PGPGPGPAEP VASGPRPWAE 100
    QLRARHLEAL RRQLHVELKV KQGAENMTHT CASGTPKERK LLAAAQQMLR 150
    DSQLKVALLR MKISSLEASG SPEPGPELLA EELQHRLHVE AAVAEGAKNV 200
    VKLLSSRRTQ DRKALAEAQA QLQESSQKLD LLRLALEQLL EQLPPAHPLR 250
    SRVTRELRAA VPGYPQPSGT PVKPTALTGT LQVRLLGCEQ LLTAVPGRSP 300
    AAALASSPSE GWLRTKAKHQ RGRGELASEV LAVLKVDNRV VGQTGWGQVA 350
    EQSWDQTFVI PLERARELEI GVHWRDWRQL CGVAFLRLED FLDNACHQLS 400
    LSLVPQGLLF AQVTFCDPVI ERRPRLQRQE RIFSKRRGQD FLRASQMNLG 450
    MAAWGRLVMN LLPPCSSPST ISPPKGCPRT PTTLREASDP ATPSNFLPKK 500
    TPLGEEMTPP PKPPRLYLPQ EPTSEETPRT KRPHMEPRTR RGPSPPASPT 550
    RKPPRLQDFR CLAVLGRGHF GKVLLVQFKG TGKYYAIKAL KKQEVLSRDE 600
    IESLYCEKRI LEAVGCTGHP FLLSLLACFQ TSSHACFVTE FVPGGDLMMQ 650
    IHEDVFPEPQ ARFYVACVVL GLQFLHEKKI IYRDLKLDNL LLDAQGFLKI 700
    ADFGLCKEGI GFGDRTSTFC GTPEFLAPEV LTQEAYTRAV DWWGLGVLLY 750
    EMLVGECPFP GDTEEEVFDC IVNMDAPYPG FLSVQGLEFI QKLLQKCPEK 800
    RLGAGEQDAE EIKVQPFFRT TNWQALLART IQPPFVPTLC GPADLRYFEG 850
    EFTGLPPALT PPAPHSLLTA RQQAAFRDFD FVSERFLEP 889
    Length:889
    Mass (Da):99,421
    Last modified:July 5, 2004 - v1
    Checksum:iF3891DA1420DF54E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti444 – 4441A → R in BAA85625. (PubMed:10441506)Curated
    Sequence conflicti627 – 6271A → V in BAA85625. (PubMed:10441506)Curated
    Sequence conflicti636 – 6361C → R in BAA85625. (PubMed:10441506)Curated
    Sequence conflicti738 – 7381R → Q in BAA85625. (PubMed:10441506)Curated
    Sequence conflicti744 – 7441G → A in BAA85625. (PubMed:10441506)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti180 – 1801A → E.1 Publication
    Corresponds to variant rs56251280 [ dbSNP | Ensembl ].
    VAR_042346
    Natural varianti404 – 4041V → L.
    Corresponds to variant rs12932 [ dbSNP | Ensembl ].
    VAR_050565

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB019692 mRNA. Translation: BAA85625.1.
    AL441992 Genomic DNA. Translation: CAI15401.1.
    BC062558 mRNA. Translation: AAH62558.1.
    CCDSiCCDS6908.1.
    PIRiJC7083.
    RefSeqiNP_037487.2. NM_013355.3.
    UniGeneiHs.300485.

    Genome annotation databases

    EnsembliENST00000291906; ENSP00000291906; ENSG00000160447.
    GeneIDi29941.
    KEGGihsa:29941.
    UCSCiuc004bvw.3. human.

    Polymorphism databases

    DMDMi74749130.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB019692 mRNA. Translation: BAA85625.1 .
    AL441992 Genomic DNA. Translation: CAI15401.1 .
    BC062558 mRNA. Translation: AAH62558.1 .
    CCDSi CCDS6908.1.
    PIRi JC7083.
    RefSeqi NP_037487.2. NM_013355.3.
    UniGenei Hs.300485.

    3D structure databases

    ProteinModelPortali Q6P5Z2.
    SMRi Q6P5Z2. Positions 27-78, 109-163, 183-241, 525-884.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 118978. 5 interactions.
    IntActi Q6P5Z2. 3 interactions.
    MINTi MINT-192027.
    STRINGi 9606.ENSP00000291906.

    PTM databases

    PhosphoSitei Q6P5Z2.

    Polymorphism databases

    DMDMi 74749130.

    Proteomic databases

    MaxQBi Q6P5Z2.
    PaxDbi Q6P5Z2.
    PRIDEi Q6P5Z2.

    Protocols and materials databases

    DNASUi 29941.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000291906 ; ENSP00000291906 ; ENSG00000160447 .
    GeneIDi 29941.
    KEGGi hsa:29941.
    UCSCi uc004bvw.3. human.

    Organism-specific databases

    CTDi 29941.
    GeneCardsi GC09P131464.
    HGNCi HGNC:17999. PKN3.
    HPAi HPA045390.
    MIMi 610714. gene.
    neXtProti NX_Q6P5Z2.
    PharmGKBi PA134919098.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233032.
    HOVERGENi HBG108317.
    InParanoidi Q6P5Z2.
    KOi K06071.
    OMAi QPFFRTT.
    OrthoDBi EOG7X9G6Q.
    PhylomeDBi Q6P5Z2.
    TreeFami TF102005.

    Enzyme and pathway databases

    SignaLinki Q6P5Z2.

    Miscellaneous databases

    GeneWikii PKN3_(gene).
    GenomeRNAii 29941.
    NextBioi 52593.
    PROi Q6P5Z2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q6P5Z2.
    Bgeei Q6P5Z2.
    CleanExi HS_PKN3.
    Genevestigatori Q6P5Z2.

    Family and domain databases

    Gene3Di 1.10.287.160. 3 hits.
    2.60.40.150. 1 hit.
    InterProi IPR000961. AGC-kinase_C.
    IPR000008. C2_dom.
    IPR011072. HR1_rho-bd.
    IPR011009. Kinase-like_dom.
    IPR017892. Pkinase_C.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF02185. HR1. 3 hits.
    PF00069. Pkinase. 1 hit.
    PF00433. Pkinase_C. 1 hit.
    [Graphical view ]
    SMARTi SM00239. C2. 1 hit.
    SM00742. Hr1. 3 hits.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46585. SSF46585. 3 hits.
    SSF49562. SSF49562. 2 hits.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of PKNbeta, a novel isoform of protein kinase PKN: expression and arachidonic acid dependency are different from those of PKNalpha."
      Oishi K., Mukai H., Shibata H., Takahashi M., Ono Y.
      Biochem. Biophys. Res. Commun. 261:808-814(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], AUTOPHOSPHORYLATION, MUTAGENESIS OF LYS-588, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Cervix carcinoma.
    2. "DNA sequence and analysis of human chromosome 9."
      Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
      , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
      Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Ovary.
    4. Cited for: FUNCTION IN MALIGNANT CELL GROWTH, PHOSPHORYLATION AT THR-718, MUTAGENESIS OF LYS-588 AND THR-718.
    5. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-544; THR-722 AND THR-860, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-544 AND SER-548, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-544 AND THR-860, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Patterns of somatic mutation in human cancer genomes."
      Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
      , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
      Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT [LARGE SCALE ANALYSIS] GLU-180.

    Entry informationi

    Entry nameiPKN3_HUMAN
    AccessioniPrimary (citable) accession number: Q6P5Z2
    Secondary accession number(s): Q9UM03
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 9
      Human chromosome 9: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3