ID   NEST_MOUSE              Reviewed;        1864 AA.
AC   Q6P5H2; A1E2I2; Q80X00; Q8BPH7; Q9CV43; Q9R0C4;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   27-MAR-2024, entry version 143.
DE   RecName: Full=Nestin;
GN   Name=Nes;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC   STRAIN=BALB/cJ;
RX   PubMed=10842089; DOI=10.1016/s0925-4773(00)00301-4;
RA   Yang J., Bian W., Gao X., Chen L., Jing N.;
RT   "Nestin expression during mouse eye and lens development.";
RL   Mech. Dev. 94:287-291(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=C57BL/6J; TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-764 AND 1510-1864.
RC   STRAIN=C57BL/6J; TISSUE=Tongue;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 33-57.
RC   TISSUE=Heart;
RX   PubMed=7542682; DOI=10.1177/43.8.7542682;
RA   Kachinsky A.M., Dominov J.A., Miller J.B.;
RT   "Intermediate filaments in cardiac myogenesis: nestin in the developing
RT   mouse heart.";
RL   J. Histochem. Cytochem. 43:843-847(1995).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1358-1514.
RC   STRAIN=VM;
RA   Huysentruyt L.C., Banerjee D., Seyfried T.N.;
RT   "Novel metastatic mouse tumor cells express multiple properties of
RT   macrophages.";
RL   Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1541, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Embryonic brain;
RX   PubMed=15345747; DOI=10.1074/mcp.m400085-mcp200;
RA   Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.;
RT   "Phosphoproteomic analysis of the developing mouse brain.";
RL   Mol. Cell. Proteomics 3:1093-1101(2004).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1837, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA   Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of mouse liver.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-623; SER-731; SER-775;
RP   SER-862; SER-894; SER-963; SER-1021; SER-1216; SER-1541 AND SER-1565, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, and
RC   Pancreas;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
RN   [9]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=20963821; DOI=10.1002/stem.541;
RA   Park D., Xiang A.P., Mao F.F., Zhang L., Di C.G., Liu X.M., Shao Y.,
RA   Ma B.F., Lee J.H., Ha K.S., Walton N., Lahn B.T.;
RT   "Nestin is required for the proper self-renewal of neural stem cells.";
RL   Stem Cells 28:2162-2171(2010).
CC   -!- FUNCTION: Required for brain and eye development. Promotes the
CC       disassembly of phosphorylated vimentin intermediate filaments (IF)
CC       during mitosis and may play a role in the trafficking and distribution
CC       of IF proteins and other cellular factors to daughter cells during
CC       progenitor cell division (By similarity). Required for survival,
CC       renewal and mitogen-stimulated proliferation of neural progenitor
CC       cells. {ECO:0000250, ECO:0000269|PubMed:20963821}.
CC   -!- SUBUNIT: Forms homodimers and homotetramers in vitro. In mixtures with
CC       other intermediate filament proteins such as vimentin and alpha-
CC       internexin, this protein preferentially forms heterodimers which can
CC       assemble to form intermediate filaments if nestin does not exceed 25%.
CC       Interacts with FHOD3 (By similarity). {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q6P5H2-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q6P5H2-2; Sequence=VSP_024923;
CC   -!- PTM: Constitutively phosphorylated. This increases during mitosis when
CC       the cytoplasmic intermediate filament network is reorganized (By
CC       similarity). {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Embryonic lethality with the neuroepithelium of
CC       developing neural tube exhibiting low numbers of neural stem cells and
CC       high levels of apoptosis. No effect on cytoskeletal integrity.
CC       {ECO:0000269|PubMed:20963821}.
CC   -!- SIMILARITY: Belongs to the intermediate filament family.
CC       {ECO:0000255|PROSITE-ProRule:PRU01188}.
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DR   EMBL; AF076623; AAF04456.2; -; mRNA.
DR   EMBL; BC060693; AAH60693.1; -; mRNA.
DR   EMBL; BC062893; AAH62893.1; -; mRNA.
DR   EMBL; AK009706; BAB26451.1; -; mRNA.
DR   EMBL; AK075690; BAC35892.1; -; mRNA.
DR   EMBL; S78708; AAP32014.1; -; mRNA.
DR   EMBL; EF101559; ABK96808.1; -; mRNA.
DR   CCDS; CCDS17461.1; -. [Q6P5H2-1]
DR   RefSeq; NP_057910.3; NM_016701.3. [Q6P5H2-1]
DR   AlphaFoldDB; Q6P5H2; -.
DR   SMR; Q6P5H2; -.
DR   BioGRID; 201730; 18.
DR   IntAct; Q6P5H2; 11.
DR   MINT; Q6P5H2; -.
DR   STRING; 10090.ENSMUSP00000088493; -.
DR   GlyGen; Q6P5H2; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; Q6P5H2; -.
DR   PhosphoSitePlus; Q6P5H2; -.
DR   EPD; Q6P5H2; -.
DR   jPOST; Q6P5H2; -.
DR   MaxQB; Q6P5H2; -.
DR   PaxDb; 10090-ENSMUSP00000088493; -.
DR   PeptideAtlas; Q6P5H2; -.
DR   ProteomicsDB; 252809; -. [Q6P5H2-1]
DR   ProteomicsDB; 252810; -. [Q6P5H2-2]
DR   Pumba; Q6P5H2; -.
DR   Antibodypedia; 1658; 1218 antibodies from 49 providers.
DR   Ensembl; ENSMUST00000090973.12; ENSMUSP00000088493.6; ENSMUSG00000004891.17. [Q6P5H2-1]
DR   Ensembl; ENSMUST00000160694.2; ENSMUSP00000125571.2; ENSMUSG00000004891.17. [Q6P5H2-2]
DR   GeneID; 18008; -.
DR   KEGG; mmu:18008; -.
DR   UCSC; uc008ptm.1; mouse. [Q6P5H2-2]
DR   UCSC; uc008ptn.1; mouse. [Q6P5H2-1]
DR   AGR; MGI:101784; -.
DR   MGI; MGI:101784; Nes.
DR   VEuPathDB; HostDB:ENSMUSG00000004891; -.
DR   eggNOG; ENOG502RYFK; Eukaryota.
DR   GeneTree; ENSGT00940000162240; -.
DR   HOGENOM; CLU_003317_0_0_1; -.
DR   InParanoid; Q6P5H2; -.
DR   OMA; RKEGWDP; -.
DR   OrthoDB; 4204342at2759; -.
DR   PhylomeDB; Q6P5H2; -.
DR   TreeFam; TF336633; -.
DR   BioGRID-ORCS; 18008; 4 hits in 77 CRISPR screens.
DR   ChiTaRS; Nes; mouse.
DR   PRO; PR:Q6P5H2; -.
DR   Proteomes; UP000000589; Chromosome 3.
DR   RNAct; Q6P5H2; Protein.
DR   Bgee; ENSMUSG00000004891; Expressed in floor plate of midbrain and 252 other cell types or tissues.
DR   GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR   GO; GO:0005882; C:intermediate filament; IDA:MGI.
DR   GO; GO:0045111; C:intermediate filament cytoskeleton; ISO:MGI.
DR   GO; GO:0031730; F:CCR5 chemokine receptor binding; ISO:MGI.
DR   GO; GO:0019215; F:intermediate filament binding; ISS:UniProtKB.
DR   GO; GO:0007420; P:brain development; ISS:UniProtKB.
DR   GO; GO:0048858; P:cell projection morphogenesis; IMP:MGI.
DR   GO; GO:0031076; P:embryonic camera-type eye development; ISS:UniProtKB.
DR   GO; GO:0000086; P:G2/M transition of mitotic cell cycle; ISO:MGI.
DR   GO; GO:0043524; P:negative regulation of neuron apoptotic process; IMP:MGI.
DR   GO; GO:0007399; P:nervous system development; IDA:MGI.
DR   GO; GO:0051402; P:neuron apoptotic process; IMP:MGI.
DR   GO; GO:0030844; P:positive regulation of intermediate filament depolymerization; ISS:UniProtKB.
DR   GO; GO:2000179; P:positive regulation of neural precursor cell proliferation; ISS:UniProtKB.
DR   GO; GO:0072089; P:stem cell proliferation; ISO:MGI.
DR   Gene3D; 1.20.5.170; -; 1.
DR   Gene3D; 1.20.5.1160; Vasodilator-stimulated phosphoprotein; 1.
DR   InterPro; IPR018039; IF_conserved.
DR   InterPro; IPR039008; IF_rod_dom.
DR   InterPro; IPR031211; Nestin.
DR   PANTHER; PTHR47051; NESTIN; 1.
DR   PANTHER; PTHR47051:SF1; NESTIN; 1.
DR   Pfam; PF00038; Filament; 1.
DR   SMART; SM01391; Filament; 1.
DR   SUPFAM; SSF64593; Intermediate filament protein, coiled coil region; 2.
DR   PROSITE; PS00226; IF_ROD_1; 1.
DR   PROSITE; PS51842; IF_ROD_2; 1.
DR   Genevisible; Q6P5H2; MM.
PE   1: Evidence at protein level;
KW   Acetylation; Alternative splicing; Coiled coil; Developmental protein;
KW   Intermediate filament; Neurogenesis; Phosphoprotein; Reference proteome.
FT   CHAIN           1..1864
FT                   /note="Nestin"
FT                   /id="PRO_0000285856"
FT   DOMAIN          8..314
FT                   /note="IF rod"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01188"
FT   REGION          1..7
FT                   /note="Head"
FT   REGION          8..43
FT                   /note="Coil 1A"
FT   REGION          44..55
FT                   /note="Linker 1"
FT   REGION          56..151
FT                   /note="Coil 1B"
FT   REGION          152..174
FT                   /note="Linker 12"
FT   REGION          175..193
FT                   /note="Coil 2A"
FT   REGION          194..196
FT                   /note="Linker 2"
FT   REGION          197..314
FT                   /note="Coil 2B"
FT   REGION          315..1864
FT                   /note="Tail"
FT   REGION          437..492
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          515..625
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          680..845
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          916..1113
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1129..1158
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1175..1344
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1375..1722
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1735..1807
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1841..1864
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        469..484
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        515..534
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        549..571
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        572..597
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        606..624
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        680..841
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        938..978
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        985..1021
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1035..1051
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1059..1087
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1088..1113
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1200..1217
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1254..1277
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1375..1403
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1439..1462
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1512..1534
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1656..1680
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1735..1754
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1787..1801
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000250|UniProtKB:P48681"
FT   MOD_RES         312
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P48681"
FT   MOD_RES         316
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:P48681"
FT   MOD_RES         356
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P48681"
FT   MOD_RES         359
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P48681"
FT   MOD_RES         389
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:P48681"
FT   MOD_RES         565
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P48681"
FT   MOD_RES         575
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P48681"
FT   MOD_RES         623
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         688
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P48681"
FT   MOD_RES         731
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         775
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         841
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P48681"
FT   MOD_RES         862
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         894
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         963
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         1010
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P21263"
FT   MOD_RES         1021
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         1106
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P48681"
FT   MOD_RES         1127
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P21263"
FT   MOD_RES         1177
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P48681"
FT   MOD_RES         1188
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P48681"
FT   MOD_RES         1195
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P48681"
FT   MOD_RES         1216
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         1290
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P48681"
FT   MOD_RES         1541
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:15345747,
FT                   ECO:0007744|PubMed:21183079"
FT   MOD_RES         1565
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         1656
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P48681"
FT   MOD_RES         1665
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P48681"
FT   MOD_RES         1745
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P48681"
FT   MOD_RES         1747
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P48681"
FT   MOD_RES         1837
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17242355"
FT   MOD_RES         1860
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P48681"
FT   MOD_RES         1861
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P48681"
FT   VAR_SEQ         750..793
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:10842089"
FT                   /id="VSP_024923"
FT   CONFLICT        184
FT                   /note="G -> C (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        257
FT                   /note="A -> P (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        265
FT                   /note="V -> L (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        319
FT                   /note="R -> G (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        458
FT                   /note="T -> S (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        535
FT                   /note="M -> K (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        544
FT                   /note="E -> K (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        558
FT                   /note="G -> R (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        570
FT                   /note="S -> L (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        587
FT                   /note="L -> S (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        673
FT                   /note="M -> I (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        704
FT                   /note="Q -> L (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        734
FT                   /note="E -> D (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        851
FT                   /note="L -> P (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        855
FT                   /note="K -> R (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        900
FT                   /note="E -> D (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1008..1011
FT                   /note="RKSL -> GKFF (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1021
FT                   /note="S -> F (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1157
FT                   /note="C -> S (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1241
FT                   /note="E -> EV (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1382
FT                   /note="G -> A (in Ref. 5; ABK96808)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1404
FT                   /note="A -> T (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1410
FT                   /note="G -> S (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1577
FT                   /note="F -> S (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1586..1591
FT                   /note="GWSPAA -> DWGPAV (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1684
FT                   /note="T -> A (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1821
FT                   /note="G -> D (in Ref. 1; AAF04456)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1864 AA;  207124 MW;  B9DF21005D977983 CRC64;
     MEGCVGEESF QMWELNRRLE AYLTRVKTLE EQNQLLSAEL GGLRAQSGDA SWRARADDEL
     AALRVLVDQR WREKHEAEVQ RDNLAEELES VAGRCQQVRL ARERTIEEAA CSRRALEAEK
     NARGWLSTQA AELERELEAL RASHEEERAH LNAQAACTPR RPPAPAHASP IRAPEVEELA
     RRLGEVWRGA VRDYQERVAH MESSLGQARE RLGQAVRGAR ESRLEVQQLQ ADRDSLQERR
     EALEQRLEGR WQDRLQATEK FQLAVEALEQ EKQGLQSQIA QILEGGQQLA HLKMSLSLEV
     ATYRTLLEAE NSRLQTPGRS SQASLGFPDP KLKLHFLGIP EDQHLGSVLP VLSPTSFSSP
     LPNTLETPVT AFLKTQEFLK ARTPTLASTP IPPMSEAPYP KNAEVRAQDV PHSLLQGGRQ
     QAPEPLWAEA TVPSSTGVLP ELEEPGGEQP DHFPDDPTSL APPLNPHHSI LEAKDRESSE
     SRVSSIFQEE EGQIWELVKK EAATEVKVEN SLAQEIQESG LDTEEIQDSQ GPLQMETLEA
     LGDEPLMSLK TQNHETPGKE NCNSSIEENS GTVKSPEKEK QTPLKSLEEK NVEAEKTLEN
     GVLELSKPLG EEEPRMEDQE LMSPEHTLET VSFLGKENQE VVRSSEEQNL ESLITFKEES
     QYPLGGPEAE DQMLERLVEK EDQRFPRSPE EDQQAFRPLE KENQEPLRFE EAEDQVLERL
     IEKERQESLK SPEEEDQQAF RLLEKENQEP LRFEDAEDQV LERLIEKERQ ESLKSPEEED
     QQAFRLLEKE NQEPLRFEEA EDQVLERLVE KESQESLKSP EEEDQRTGKP LEKENQESLR
     SLDENQETIV LLESKNQRPL RSLEVEEEEQ RIVKPLEKVS QVSLESLEKE NVQSPRYLEE
     DDHMIKSLLE DKTHEILGSL EDRNGENFIP PENETQGSLR PPEEEDQRIV NHLEKESQEF
     LRSPEAEEEE EQVMVRSLEG ENHDPLSSVV KEEQMAESKL ENESQDSRKS LEDESQETFG
     SLEKENLESL RSLAGQDQEE QKLEQETQQP LRAVEDEQMT VNPPEKVDPE LPKPLRNDQE
     VVRSLDKENQ ESLVSLNEGG METVKSSETE NIESLETVGE CLGRRKSVDT QEPLWSTEVT
     SETIEPLEDE TQEPLGCVDE NQEVLTPLER ESQELRSLGK WNPETVESPG GVEDSQQCLE
     VEEGPEREQH QESLRSLGEV EWELPGSGSQ QRWEDVVEDG EGQEASLGAT GVETEDKAEL
     HLRGQGGEEK AVEEGELLQD AVGEAWSLGS SEPKEQRVPA EPLDDLEGQP EQTGTLEVPV
     AQGMPEATEQ DEDRAQAGEQ DSVEVTLGLE AARAGLELEQ EVVGLEDPRH FAREEAIHPS
     LGEESVKAKI DQGLEEPGKE PKEAGALDSG IPELPKTSSE TLECKGWEES GEGWGEEEAS
     LETSDHEGSH APQPRPPKTE EDEGLQAALT VPGPKLLEPC SPIPILTDAH ELQPQAEGIQ
     EAGWQPEAGT EALGRVEDEP EFGRGEIPEG LQDWEEGRED SEADELGETL PDSTPLGLYL
     KSPASPKWEQ AGEQRLFPQG EARKEGWSPA ALAAQGLSDP PEEEQQGHDS DLSSEEFEDL
     GTEASLLPGV PKEVSDHLGQ EPPVLQPACW DQGGESDGFA DEEESGEEGE EEDADEEEGA
     ESGTQWWGPG PSGGGVKVQD VTQRGDLEHE SVGDSGLWDD GLSGAAANVL VTALETVSQD
     SAEPSGSEGS ESASLEGEEG QAIDHLDAPQ EVTSVVPGAG DTFDISGQGP NLESEQVNGR
     MENGLEQAEG QVVLHGDEDQ GIPLQEQGTL KAPLVGSPVH LGPSQPLKFT LSGVDGDSWS
     SGED
//