Q6P5H2 (NEST_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nestin

Gene names

Name:

Nes

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1864 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division By similarity. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. Ref.8
Subunit structure	Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3 By similarity.
Post-translational modification	Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized By similarity.
Disruption phenotype	Embryonic lethality with the neuroepithelium of developing neural tube exhibiting low numbers of neural stem cells and high levels of apoptosis. No effect on cytoskeletal integrity. Ref.8
Sequence similarities	Belongs to the intermediate filament family.

Ontologies

Keywords
Biological process	Neurogenesis
Cellular component	Intermediate filament
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G2/M transition of mitotic cell cycle Inferred from electronic annotation. Source: Compara brain development Inferred from sequence or structural similarity. Source: UniProtKB cell projection morphogenesis Inferred from mutant phenotype PubMed 16571784. Source: MGI embryonic camera-type eye development Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of apoptotic process Inferred from mutant phenotype Ref.8. Source: UniProtKB negative regulation of catalytic activity Inferred from electronic annotation. Source: Compara negative regulation of protein binding Inferred from electronic annotation. Source: Compara positive regulation of intermediate filament depolymerization Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of neural precursor cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB stem cell proliferation Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from direct assay PubMed 14706696PubMed 17901127PubMed 18614158PubMed 19036983PubMed 19409199. Source: MGI intermediate filament Inferred from direct assay PubMed 9364068. Source: MGI
Molecular_function	intermediate filament binding Inferred from sequence or structural similarity. Source: UniProtKB structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6P5H2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6P5H2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     750-793: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1864	1864	Nestin		PRO_0000285856
Regions
	Region	1 – 7	7	Head
	Region	8 – 314	307	Rod
	Region	8 – 43	36	Coil 1A
	Region	44 – 55	12	Linker 1
	Region	56 – 151	96	Coil 1B
	Region	152 – 174	23	Linker 12
	Region	175 – 193	19	Coil 2A
	Region	194 – 196	3	Linker 2
	Region	197 – 314	118	Coil 2B
	Region	315 – 1864	1550	Tail
Amino acid modifications
	Modified residue	312	1	Phosphoserine By similarity
	Modified residue	316	1	Phosphothreonine By similarity
	Modified residue	356	1	Phosphoserine By similarity
	Modified residue	359	1	Phosphoserine By similarity
	Modified residue	389	1	Phosphothreonine By similarity
	Modified residue	565	1	Phosphoserine By similarity
	Modified residue	575	1	Phosphoserine By similarity
	Modified residue	688	1	Phosphoserine Ref.7
	Modified residue	841	1	Phosphoserine By similarity
	Modified residue	1106	1	Phosphoserine By similarity
	Modified residue	1177	1	Phosphoserine By similarity
	Modified residue	1188	1	Phosphoserine By similarity
	Modified residue	1195	1	Phosphoserine By similarity
	Modified residue	1216	1	Phosphoserine By similarity
	Modified residue	1541	1	Phosphoserine Ref.6
	Modified residue	1656	1	Phosphoserine By similarity
	Modified residue	1665	1	Phosphoserine By similarity
	Modified residue	1745	1	Phosphoserine By similarity
	Modified residue	1747	1	Phosphoserine By similarity
	Modified residue	1860	1	Phosphoserine By similarity
	Modified residue	1861	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	750 – 793	44	Missing in isoform 2.		VSP_024923
Experimental info
	Sequence conflict	184	1	G → C in AAF04456. Ref.1
	Sequence conflict	257	1	A → P in AAF04456. Ref.1
	Sequence conflict	265	1	V → L in AAF04456. Ref.1
	Sequence conflict	319	1	R → G in AAF04456. Ref.1
	Sequence conflict	458	1	T → S in AAF04456. Ref.1
	Sequence conflict	535	1	M → K in AAF04456. Ref.1
	Sequence conflict	544	1	E → K in AAF04456. Ref.1
	Sequence conflict	558	1	G → R in AAF04456. Ref.1
	Sequence conflict	570	1	S → L in AAF04456. Ref.1
	Sequence conflict	587	1	L → S in AAF04456. Ref.1
	Sequence conflict	673	1	M → I in AAF04456. Ref.1
	Sequence conflict	704	1	Q → L in AAF04456. Ref.1
	Sequence conflict	734	1	E → D in AAF04456. Ref.1
	Sequence conflict	851	1	L → P in AAF04456. Ref.1
	Sequence conflict	855	1	K → R in AAF04456. Ref.1
	Sequence conflict	900	1	E → D in AAF04456. Ref.1
	Sequence conflict	1008 – 1011	4	RKSL → GKFF in AAF04456. Ref.1
	Sequence conflict	1021	1	S → F in AAF04456. Ref.1
	Sequence conflict	1157	1	C → S in AAF04456. Ref.1
	Sequence conflict	1241	1	E → EV in AAF04456. Ref.1
	Sequence conflict	1382	1	G → A in ABK96808. Ref.5
	Sequence conflict	1404	1	A → T in AAF04456. Ref.1
	Sequence conflict	1410	1	G → S in AAF04456. Ref.1
	Sequence conflict	1577	1	F → S in AAF04456. Ref.1
	Sequence conflict	1586 – 1591	6	GWSPAA → DWGPAV in AAF04456. Ref.1
	Sequence conflict	1684	1	T → A in AAF04456. Ref.1
	Sequence conflict	1821	1	G → D in AAF04456. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: B9DF21005D977983	FASTA	1,864	207,124
	10 20 30 40 50 60 MEGCVGEESF QMWELNRRLE AYLTRVKTLE EQNQLLSAEL GGLRAQSGDA SWRARADDEL 70 80 90 100 110 120 AALRVLVDQR WREKHEAEVQ RDNLAEELES VAGRCQQVRL ARERTIEEAA CSRRALEAEK 130 140 150 160 170 180 NARGWLSTQA AELERELEAL RASHEEERAH LNAQAACTPR RPPAPAHASP IRAPEVEELA 190 200 210 220 230 240 RRLGEVWRGA VRDYQERVAH MESSLGQARE RLGQAVRGAR ESRLEVQQLQ ADRDSLQERR 250 260 270 280 290 300 EALEQRLEGR WQDRLQATEK FQLAVEALEQ EKQGLQSQIA QILEGGQQLA HLKMSLSLEV 310 320 330 340 350 360 ATYRTLLEAE NSRLQTPGRS SQASLGFPDP KLKLHFLGIP EDQHLGSVLP VLSPTSFSSP 370 380 390 400 410 420 LPNTLETPVT AFLKTQEFLK ARTPTLASTP IPPMSEAPYP KNAEVRAQDV PHSLLQGGRQ 430 440 450 460 470 480 QAPEPLWAEA TVPSSTGVLP ELEEPGGEQP DHFPDDPTSL APPLNPHHSI LEAKDRESSE 490 500 510 520 530 540 SRVSSIFQEE EGQIWELVKK EAATEVKVEN SLAQEIQESG LDTEEIQDSQ GPLQMETLEA 550 560 570 580 590 600 LGDEPLMSLK TQNHETPGKE NCNSSIEENS GTVKSPEKEK QTPLKSLEEK NVEAEKTLEN 610 620 630 640 650 660 GVLELSKPLG EEEPRMEDQE LMSPEHTLET VSFLGKENQE VVRSSEEQNL ESLITFKEES 670 680 690 700 710 720 QYPLGGPEAE DQMLERLVEK EDQRFPRSPE EDQQAFRPLE KENQEPLRFE EAEDQVLERL 730 740 750 760 770 780 IEKERQESLK SPEEEDQQAF RLLEKENQEP LRFEDAEDQV LERLIEKERQ ESLKSPEEED 790 800 810 820 830 840 QQAFRLLEKE NQEPLRFEEA EDQVLERLVE KESQESLKSP EEEDQRTGKP LEKENQESLR 850 860 870 880 890 900 SLDENQETIV LLESKNQRPL RSLEVEEEEQ RIVKPLEKVS QVSLESLEKE NVQSPRYLEE 910 920 930 940 950 960 DDHMIKSLLE DKTHEILGSL EDRNGENFIP PENETQGSLR PPEEEDQRIV NHLEKESQEF 970 980 990 1000 1010 1020 LRSPEAEEEE EQVMVRSLEG ENHDPLSSVV KEEQMAESKL ENESQDSRKS LEDESQETFG 1030 1040 1050 1060 1070 1080 SLEKENLESL RSLAGQDQEE QKLEQETQQP LRAVEDEQMT VNPPEKVDPE LPKPLRNDQE 1090 1100 1110 1120 1130 1140 VVRSLDKENQ ESLVSLNEGG METVKSSETE NIESLETVGE CLGRRKSVDT QEPLWSTEVT 1150 1160 1170 1180 1190 1200 SETIEPLEDE TQEPLGCVDE NQEVLTPLER ESQELRSLGK WNPETVESPG GVEDSQQCLE 1210 1220 1230 1240 1250 1260 VEEGPEREQH QESLRSLGEV EWELPGSGSQ QRWEDVVEDG EGQEASLGAT GVETEDKAEL 1270 1280 1290 1300 1310 1320 HLRGQGGEEK AVEEGELLQD AVGEAWSLGS SEPKEQRVPA EPLDDLEGQP EQTGTLEVPV 1330 1340 1350 1360 1370 1380 AQGMPEATEQ DEDRAQAGEQ DSVEVTLGLE AARAGLELEQ EVVGLEDPRH FAREEAIHPS 1390 1400 1410 1420 1430 1440 LGEESVKAKI DQGLEEPGKE PKEAGALDSG IPELPKTSSE TLECKGWEES GEGWGEEEAS 1450 1460 1470 1480 1490 1500 LETSDHEGSH APQPRPPKTE EDEGLQAALT VPGPKLLEPC SPIPILTDAH ELQPQAEGIQ 1510 1520 1530 1540 1550 1560 EAGWQPEAGT EALGRVEDEP EFGRGEIPEG LQDWEEGRED SEADELGETL PDSTPLGLYL 1570 1580 1590 1600 1610 1620 KSPASPKWEQ AGEQRLFPQG EARKEGWSPA ALAAQGLSDP PEEEQQGHDS DLSSEEFEDL 1630 1640 1650 1660 1670 1680 GTEASLLPGV PKEVSDHLGQ EPPVLQPACW DQGGESDGFA DEEESGEEGE EEDADEEEGA 1690 1700 1710 1720 1730 1740 ESGTQWWGPG PSGGGVKVQD VTQRGDLEHE SVGDSGLWDD GLSGAAANVL VTALETVSQD 1750 1760 1770 1780 1790 1800 SAEPSGSEGS ESASLEGEEG QAIDHLDAPQ EVTSVVPGAG DTFDISGQGP NLESEQVNGR 1810 1820 1830 1840 1850 1860 MENGLEQAEG QVVLHGDEDQ GIPLQEQGTL KAPLVGSPVH LGPSQPLKFT LSGVDGDSWS SGED
	Isoform 2 [UniParc]. Checksum: 270C501DACA10100	FASTA	1,820	201,770
	10 20 30 40 50 60 MEGCVGEESF QMWELNRRLE AYLTRVKTLE EQNQLLSAEL GGLRAQSGDA SWRARADDEL 70 80 90 100 110 120 AALRVLVDQR WREKHEAEVQ RDNLAEELES VAGRCQQVRL ARERTIEEAA CSRRALEAEK 130 140 150 160 170 180 NARGWLSTQA AELERELEAL RASHEEERAH LNAQAACTPR RPPAPAHASP IRAPEVEELA 190 200 210 220 230 240 RRLGEVWRGA VRDYQERVAH MESSLGQARE RLGQAVRGAR ESRLEVQQLQ ADRDSLQERR 250 260 270 280 290 300 EALEQRLEGR WQDRLQATEK FQLAVEALEQ EKQGLQSQIA QILEGGQQLA HLKMSLSLEV 310 320 330 340 350 360 ATYRTLLEAE NSRLQTPGRS SQASLGFPDP KLKLHFLGIP EDQHLGSVLP VLSPTSFSSP 370 380 390 400 410 420 LPNTLETPVT AFLKTQEFLK ARTPTLASTP IPPMSEAPYP KNAEVRAQDV PHSLLQGGRQ 430 440 450 460 470 480 QAPEPLWAEA TVPSSTGVLP ELEEPGGEQP DHFPDDPTSL APPLNPHHSI LEAKDRESSE 490 500 510 520 530 540 SRVSSIFQEE EGQIWELVKK EAATEVKVEN SLAQEIQESG LDTEEIQDSQ GPLQMETLEA 550 560 570 580 590 600 LGDEPLMSLK TQNHETPGKE NCNSSIEENS GTVKSPEKEK QTPLKSLEEK NVEAEKTLEN 610 620 630 640 650 660 GVLELSKPLG EEEPRMEDQE LMSPEHTLET VSFLGKENQE VVRSSEEQNL ESLITFKEES 670 680 690 700 710 720 QYPLGGPEAE DQMLERLVEK EDQRFPRSPE EDQQAFRPLE KENQEPLRFE EAEDQVLERL 730 740 750 760 770 780 IEKERQESLK SPEEEDQQAF RLLEKENQEP LRFEEAEDQV LERLVEKESQ ESLKSPEEED 790 800 810 820 830 840 QRTGKPLEKE NQESLRSLDE NQETIVLLES KNQRPLRSLE VEEEEQRIVK PLEKVSQVSL 850 860 870 880 890 900 ESLEKENVQS PRYLEEDDHM IKSLLEDKTH EILGSLEDRN GENFIPPENE TQGSLRPPEE 910 920 930 940 950 960 EDQRIVNHLE KESQEFLRSP EAEEEEEQVM VRSLEGENHD PLSSVVKEEQ MAESKLENES 970 980 990 1000 1010 1020 QDSRKSLEDE SQETFGSLEK ENLESLRSLA GQDQEEQKLE QETQQPLRAV EDEQMTVNPP 1030 1040 1050 1060 1070 1080 EKVDPELPKP LRNDQEVVRS LDKENQESLV SLNEGGMETV KSSETENIES LETVGECLGR 1090 1100 1110 1120 1130 1140 RKSVDTQEPL WSTEVTSETI EPLEDETQEP LGCVDENQEV LTPLERESQE LRSLGKWNPE 1150 1160 1170 1180 1190 1200 TVESPGGVED SQQCLEVEEG PEREQHQESL RSLGEVEWEL PGSGSQQRWE DVVEDGEGQE 1210 1220 1230 1240 1250 1260 ASLGATGVET EDKAELHLRG QGGEEKAVEE GELLQDAVGE AWSLGSSEPK EQRVPAEPLD 1270 1280 1290 1300 1310 1320 DLEGQPEQTG TLEVPVAQGM PEATEQDEDR AQAGEQDSVE VTLGLEAARA GLELEQEVVG 1330 1340 1350 1360 1370 1380 LEDPRHFARE EAIHPSLGEE SVKAKIDQGL EEPGKEPKEA GALDSGIPEL PKTSSETLEC 1390 1400 1410 1420 1430 1440 KGWEESGEGW GEEEASLETS DHEGSHAPQP RPPKTEEDEG LQAALTVPGP KLLEPCSPIP 1450 1460 1470 1480 1490 1500 ILTDAHELQP QAEGIQEAGW QPEAGTEALG RVEDEPEFGR GEIPEGLQDW EEGREDSEAD 1510 1520 1530 1540 1550 1560 ELGETLPDST PLGLYLKSPA SPKWEQAGEQ RLFPQGEARK EGWSPAALAA QGLSDPPEEE 1570 1580 1590 1600 1610 1620 QQGHDSDLSS EEFEDLGTEA SLLPGVPKEV SDHLGQEPPV LQPACWDQGG ESDGFADEEE 1630 1640 1650 1660 1670 1680 SGEEGEEEDA DEEEGAESGT QWWGPGPSGG GVKVQDVTQR GDLEHESVGD SGLWDDGLSG 1690 1700 1710 1720 1730 1740 AAANVLVTAL ETVSQDSAEP SGSEGSESAS LEGEEGQAID HLDAPQEVTS VVPGAGDTFD 1750 1760 1770 1780 1790 1800 ISGQGPNLES EQVNGRMENG LEQAEGQVVL HGDEDQGIPL QEQGTLKAPL VGSPVHLGPS 1810 1820 QPLKFTLSGV DGDSWSSGED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nestin expression during mouse eye and lens development." Yang J., Bian W., Gao X., Chen L., Jing N. Mech. Dev. 94:287-291(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: BALB/c.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-764 AND 1510-1864. Strain: C57BL/6J. Tissue: Tongue.
[4]	"Intermediate filaments in cardiac myogenesis: nestin in the developing mouse heart." Kachinsky A.M., Dominov J.A., Miller J.B. J. Histochem. Cytochem. 43:843-847(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-57. Tissue: Heart.
[5]	"Novel metastatic mouse tumor cells express multiple properties of macrophages." Huysentruyt L.C., Banerjee D., Seyfried T.N. Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1358-1514. Strain: VM.
[6]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1541, MASS SPECTROMETRY. Tissue: Embryonic brain.
[7]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-688, MASS SPECTROMETRY. Tissue: Melanoma.
[8]	"Nestin is required for the proper self-renewal of neural stem cells." Park D., Xiang A.P., Mao F.F., Zhang L., Di C.G., Liu X.M., Shao Y., Ma B.F., Lee J.H., Ha K.S., Walton N., Lahn B.T. Stem Cells 28:2162-2171(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF076623 mRNA. Translation: AAF04456.2. BC060693 mRNA. Translation: AAH60693.1. BC062893 mRNA. Translation: AAH62893.1. AK009706 mRNA. Translation: BAB26451.1. AK075690 mRNA. Translation: BAC35892.1. S78708 mRNA. Translation: AAP32014.1. EF101559 mRNA. Translation: ABK96808.1.
IPI	IPI00453692. IPI00845608.
RefSeq	NP_057910.3. NM_016701.3.
UniGene	Mm.331129.
3D structure databases
ProteinModelPortal	Q6P5H2.
SMR	Q6P5H2. Positions 6-154, 221-311.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6P5H2. 4 interactions.
PTM databases
PhosphoSite	Q6P5H2.
Proteomic databases
PaxDb	Q6P5H2.
PRIDE	Q6P5H2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000090973; ENSMUSP00000088493; ENSMUSG00000004891. ENSMUST00000160694; ENSMUSP00000125571; ENSMUSG00000004891.
GeneID	18008.
KEGG	mmu:18008.
UCSC	uc008ptm.1. mouse. uc008ptn.1. mouse.
Organism-specific databases
CTD	10763.
MGI	MGI:101784. Nes.
Phylogenomic databases
eggNOG	NOG12793.
GeneTree	ENSGT00560000076873.
HOGENOM	HOG000113766.
HOVERGEN	HBG006463.
InParanoid	Q6P5H2.
KO	K07609.
OMA	PGKENQE.
OrthoDB	EOG49GKHK.
Gene expression databases
Bgee	Q6P5H2.
CleanEx	MM_NES.
Genevestigator	Q6P5H2.
Family and domain databases
InterPro	IPR016044. F. IPR001664. IF. IPR018039. Intermediate_filament_CS. [Graphical view]
PANTHER	PTHR23239. PTHR23239. 1 hit.
Pfam	PF00038. Filament. 1 hit. [Graphical view]
PROSITE	PS00226. IF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	NES. mouse.
NextBio	293025.
SOURCE	Search...

Entry information

Entry name

NEST_MOUSE

Accession

Primary (citable) accession number: Q6P5H2
Secondary accession number(s): A1E2I2 , Q80X00, Q8BPH7, Q9CV43, Q9R0C4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program