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Q6P5H2 (NEST_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nestin
Gene names
Name:Nes
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1864 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division By similarity. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. Ref.8

Subunit structure

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3 By similarity.

Post-translational modification

Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized By similarity.

Disruption phenotype

Embryonic lethality with the neuroepithelium of developing neural tube exhibiting low numbers of neural stem cells and high levels of apoptosis. No effect on cytoskeletal integrity. Ref.8

Sequence similarities

Belongs to the intermediate filament family.

Ontologies

Keywords
   Biological processNeurogenesis
   Cellular componentIntermediate filament
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   Molecular functionDevelopmental protein
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG2/M transition of mitotic cell cycle

Inferred from electronic annotation. Source: Ensembl

brain development

Inferred from sequence or structural similarity. Source: UniProtKB

cell projection morphogenesis

Inferred from mutant phenotype PubMed 16571784. Source: MGI

embryonic camera-type eye development

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of catalytic activity

Inferred from electronic annotation. Source: Ensembl

negative regulation of neuron apoptotic process

Inferred from mutant phenotype Ref.8. Source: MGI

negative regulation of protein binding

Inferred from electronic annotation. Source: Ensembl

nervous system development

Inferred from direct assay PubMed 12687705. Source: MGI

positive regulation of intermediate filament depolymerization

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of neural precursor cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

stem cell proliferation

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 14706696PubMed 17901127PubMed 18614158PubMed 19036983PubMed 19409199. Source: MGI

intermediate filament

Inferred from direct assay PubMed 9364068. Source: MGI

intracellular

Inferred from direct assay PubMed 11744388PubMed 16892058. Source: MGI

   Molecular_functionintermediate filament binding

Inferred from sequence or structural similarity. Source: UniProtKB

structural molecule activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6P5H2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6P5H2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     750-793: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 18641864Nestin
PRO_0000285856

Regions

Region1 – 77Head
Region8 – 314307Rod
Region8 – 4336Coil 1A
Region44 – 5512Linker 1
Region56 – 15196Coil 1B
Region152 – 17423Linker 12
Region175 – 19319Coil 2A
Region194 – 1963Linker 2
Region197 – 314118Coil 2B
Region315 – 18641550Tail

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue3121Phosphoserine By similarity
Modified residue3161Phosphothreonine By similarity
Modified residue3561Phosphoserine By similarity
Modified residue3591Phosphoserine By similarity
Modified residue3891Phosphothreonine By similarity
Modified residue5651Phosphoserine By similarity
Modified residue5751Phosphoserine By similarity
Modified residue6881Phosphoserine By similarity
Modified residue8411Phosphoserine By similarity
Modified residue11061Phosphoserine By similarity
Modified residue11771Phosphoserine By similarity
Modified residue11881Phosphoserine By similarity
Modified residue11951Phosphoserine By similarity
Modified residue12161Phosphoserine By similarity
Modified residue15411Phosphoserine Ref.6
Modified residue16561Phosphoserine By similarity
Modified residue16651Phosphoserine By similarity
Modified residue17451Phosphoserine By similarity
Modified residue17471Phosphoserine By similarity
Modified residue18371Phosphoserine Ref.7
Modified residue18601Phosphoserine By similarity
Modified residue18611Phosphoserine By similarity

Natural variations

Alternative sequence750 – 79344Missing in isoform 2.
VSP_024923

Experimental info

Sequence conflict1841G → C in AAF04456. Ref.1
Sequence conflict2571A → P in AAF04456. Ref.1
Sequence conflict2651V → L in AAF04456. Ref.1
Sequence conflict3191R → G in AAF04456. Ref.1
Sequence conflict4581T → S in AAF04456. Ref.1
Sequence conflict5351M → K in AAF04456. Ref.1
Sequence conflict5441E → K in AAF04456. Ref.1
Sequence conflict5581G → R in AAF04456. Ref.1
Sequence conflict5701S → L in AAF04456. Ref.1
Sequence conflict5871L → S in AAF04456. Ref.1
Sequence conflict6731M → I in AAF04456. Ref.1
Sequence conflict7041Q → L in AAF04456. Ref.1
Sequence conflict7341E → D in AAF04456. Ref.1
Sequence conflict8511L → P in AAF04456. Ref.1
Sequence conflict8551K → R in AAF04456. Ref.1
Sequence conflict9001E → D in AAF04456. Ref.1
Sequence conflict1008 – 10114RKSL → GKFF in AAF04456. Ref.1
Sequence conflict10211S → F in AAF04456. Ref.1
Sequence conflict11571C → S in AAF04456. Ref.1
Sequence conflict12411E → EV in AAF04456. Ref.1
Sequence conflict13821G → A in ABK96808. Ref.5
Sequence conflict14041A → T in AAF04456. Ref.1
Sequence conflict14101G → S in AAF04456. Ref.1
Sequence conflict15771F → S in AAF04456. Ref.1
Sequence conflict1586 – 15916GWSPAA → DWGPAV in AAF04456. Ref.1
Sequence conflict16841T → A in AAF04456. Ref.1
Sequence conflict18211G → D in AAF04456. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: B9DF21005D977983

FASTA1,864207,124
        10         20         30         40         50         60 
MEGCVGEESF QMWELNRRLE AYLTRVKTLE EQNQLLSAEL GGLRAQSGDA SWRARADDEL 

        70         80         90        100        110        120 
AALRVLVDQR WREKHEAEVQ RDNLAEELES VAGRCQQVRL ARERTIEEAA CSRRALEAEK 

       130        140        150        160        170        180 
NARGWLSTQA AELERELEAL RASHEEERAH LNAQAACTPR RPPAPAHASP IRAPEVEELA 

       190        200        210        220        230        240 
RRLGEVWRGA VRDYQERVAH MESSLGQARE RLGQAVRGAR ESRLEVQQLQ ADRDSLQERR 

       250        260        270        280        290        300 
EALEQRLEGR WQDRLQATEK FQLAVEALEQ EKQGLQSQIA QILEGGQQLA HLKMSLSLEV 

       310        320        330        340        350        360 
ATYRTLLEAE NSRLQTPGRS SQASLGFPDP KLKLHFLGIP EDQHLGSVLP VLSPTSFSSP 

       370        380        390        400        410        420 
LPNTLETPVT AFLKTQEFLK ARTPTLASTP IPPMSEAPYP KNAEVRAQDV PHSLLQGGRQ 

       430        440        450        460        470        480 
QAPEPLWAEA TVPSSTGVLP ELEEPGGEQP DHFPDDPTSL APPLNPHHSI LEAKDRESSE 

       490        500        510        520        530        540 
SRVSSIFQEE EGQIWELVKK EAATEVKVEN SLAQEIQESG LDTEEIQDSQ GPLQMETLEA 

       550        560        570        580        590        600 
LGDEPLMSLK TQNHETPGKE NCNSSIEENS GTVKSPEKEK QTPLKSLEEK NVEAEKTLEN 

       610        620        630        640        650        660 
GVLELSKPLG EEEPRMEDQE LMSPEHTLET VSFLGKENQE VVRSSEEQNL ESLITFKEES 

       670        680        690        700        710        720 
QYPLGGPEAE DQMLERLVEK EDQRFPRSPE EDQQAFRPLE KENQEPLRFE EAEDQVLERL 

       730        740        750        760        770        780 
IEKERQESLK SPEEEDQQAF RLLEKENQEP LRFEDAEDQV LERLIEKERQ ESLKSPEEED 

       790        800        810        820        830        840 
QQAFRLLEKE NQEPLRFEEA EDQVLERLVE KESQESLKSP EEEDQRTGKP LEKENQESLR 

       850        860        870        880        890        900 
SLDENQETIV LLESKNQRPL RSLEVEEEEQ RIVKPLEKVS QVSLESLEKE NVQSPRYLEE 

       910        920        930        940        950        960 
DDHMIKSLLE DKTHEILGSL EDRNGENFIP PENETQGSLR PPEEEDQRIV NHLEKESQEF 

       970        980        990       1000       1010       1020 
LRSPEAEEEE EQVMVRSLEG ENHDPLSSVV KEEQMAESKL ENESQDSRKS LEDESQETFG 

      1030       1040       1050       1060       1070       1080 
SLEKENLESL RSLAGQDQEE QKLEQETQQP LRAVEDEQMT VNPPEKVDPE LPKPLRNDQE 

      1090       1100       1110       1120       1130       1140 
VVRSLDKENQ ESLVSLNEGG METVKSSETE NIESLETVGE CLGRRKSVDT QEPLWSTEVT 

      1150       1160       1170       1180       1190       1200 
SETIEPLEDE TQEPLGCVDE NQEVLTPLER ESQELRSLGK WNPETVESPG GVEDSQQCLE 

      1210       1220       1230       1240       1250       1260 
VEEGPEREQH QESLRSLGEV EWELPGSGSQ QRWEDVVEDG EGQEASLGAT GVETEDKAEL 

      1270       1280       1290       1300       1310       1320 
HLRGQGGEEK AVEEGELLQD AVGEAWSLGS SEPKEQRVPA EPLDDLEGQP EQTGTLEVPV 

      1330       1340       1350       1360       1370       1380 
AQGMPEATEQ DEDRAQAGEQ DSVEVTLGLE AARAGLELEQ EVVGLEDPRH FAREEAIHPS 

      1390       1400       1410       1420       1430       1440 
LGEESVKAKI DQGLEEPGKE PKEAGALDSG IPELPKTSSE TLECKGWEES GEGWGEEEAS 

      1450       1460       1470       1480       1490       1500 
LETSDHEGSH APQPRPPKTE EDEGLQAALT VPGPKLLEPC SPIPILTDAH ELQPQAEGIQ 

      1510       1520       1530       1540       1550       1560 
EAGWQPEAGT EALGRVEDEP EFGRGEIPEG LQDWEEGRED SEADELGETL PDSTPLGLYL 

      1570       1580       1590       1600       1610       1620 
KSPASPKWEQ AGEQRLFPQG EARKEGWSPA ALAAQGLSDP PEEEQQGHDS DLSSEEFEDL 

      1630       1640       1650       1660       1670       1680 
GTEASLLPGV PKEVSDHLGQ EPPVLQPACW DQGGESDGFA DEEESGEEGE EEDADEEEGA 

      1690       1700       1710       1720       1730       1740 
ESGTQWWGPG PSGGGVKVQD VTQRGDLEHE SVGDSGLWDD GLSGAAANVL VTALETVSQD 

      1750       1760       1770       1780       1790       1800 
SAEPSGSEGS ESASLEGEEG QAIDHLDAPQ EVTSVVPGAG DTFDISGQGP NLESEQVNGR 

      1810       1820       1830       1840       1850       1860 
MENGLEQAEG QVVLHGDEDQ GIPLQEQGTL KAPLVGSPVH LGPSQPLKFT LSGVDGDSWS 


SGED 

« Hide

Isoform 2 [UniParc].

Checksum: 270C501DACA10100
Show »

FASTA1,820201,770

References

« Hide 'large scale' references
[1]"Nestin expression during mouse eye and lens development."
Yang J., Bian W., Gao X., Chen L., Jing N.
Mech. Dev. 94:287-291(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: BALB/c.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6.
Tissue: Brain.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-764 AND 1510-1864.
Strain: C57BL/6J.
Tissue: Tongue.
[4]"Intermediate filaments in cardiac myogenesis: nestin in the developing mouse heart."
Kachinsky A.M., Dominov J.A., Miller J.B.
J. Histochem. Cytochem. 43:843-847(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-57.
Tissue: Heart.
[5]"Novel metastatic mouse tumor cells express multiple properties of macrophages."
Huysentruyt L.C., Banerjee D., Seyfried T.N.
Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1358-1514.
Strain: VM.
[6]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1541, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.
[7]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1837, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[8]"Nestin is required for the proper self-renewal of neural stem cells."
Park D., Xiang A.P., Mao F.F., Zhang L., Di C.G., Liu X.M., Shao Y., Ma B.F., Lee J.H., Ha K.S., Walton N., Lahn B.T.
Stem Cells 28:2162-2171(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF076623 mRNA. Translation: AAF04456.2.
BC060693 mRNA. Translation: AAH60693.1.
BC062893 mRNA. Translation: AAH62893.1.
AK009706 mRNA. Translation: BAB26451.1.
AK075690 mRNA. Translation: BAC35892.1.
S78708 mRNA. Translation: AAP32014.1.
EF101559 mRNA. Translation: ABK96808.1.
RefSeqNP_057910.3. NM_016701.3.
UniGeneMm.331129.

3D structure databases

ProteinModelPortalQ6P5H2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ6P5H2. 4 interactions.

PTM databases

PhosphoSiteQ6P5H2.

Proteomic databases

PaxDbQ6P5H2.
PRIDEQ6P5H2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000090973; ENSMUSP00000088493; ENSMUSG00000004891. [Q6P5H2-1]
ENSMUST00000160694; ENSMUSP00000125571; ENSMUSG00000004891. [Q6P5H2-2]
GeneID18008.
KEGGmmu:18008.
UCSCuc008ptm.1. mouse. [Q6P5H2-2]
uc008ptn.1. mouse. [Q6P5H2-1]

Organism-specific databases

CTD10763.
MGIMGI:101784. Nes.

Phylogenomic databases

eggNOGNOG12793.
GeneTreeENSGT00730000110953.
HOGENOMHOG000113766.
HOVERGENHBG006463.
InParanoidQ6P5H2.
KOK07609.
OMADHASLAP.
OrthoDBEOG773XKP.
PhylomeDBQ6P5H2.
TreeFamTF336633.

Gene expression databases

BgeeQ6P5H2.
CleanExMM_NES.
GenevestigatorQ6P5H2.

Family and domain databases

InterProIPR001664. IF.
IPR018039. Intermediate_filament_CS.
[Graphical view]
PANTHERPTHR23239. PTHR23239. 1 hit.
PfamPF00038. Filament. 1 hit.
[Graphical view]
PROSITEPS00226. IF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNES. mouse.
NextBio293025.
PROQ6P5H2.
SOURCESearch...

Entry information

Entry nameNEST_MOUSE
AccessionPrimary (citable) accession number: Q6P5H2
Secondary accession number(s): A1E2I2 expand/collapse secondary AC list , Q80X00, Q8BPH7, Q9CV43, Q9R0C4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot