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Q6P5G0

- MK04_MOUSE

UniProt

Q6P5G0 - MK04_MOUSE

Protein

Mitogen-activated protein kinase 4

Gene

Mapk4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by phosphorylation at Ser-186.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei49 – 491ATPPROSITE-ProRule annotation
    Active sitei149 – 1491Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi26 – 349ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB-EC
    3. protein heterodimerization activity Source: UniProtKB
    4. protein homodimerization activity Source: UniProtKB
    5. protein kinase binding Source: UniProtKB

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 4 (EC:2.7.11.24)
    Short name:
    MAP kinase 4
    Short name:
    MAPK 4
    Alternative name(s):
    Extracellular signal-regulated kinase 4
    Short name:
    ERK-4
    Gene namesi
    Name:Mapk4
    Synonyms:Erk4, Prkm4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:2444559. Mapk4.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication
    Note: Translocates to the cytoplasm following interaction with MAPKAPK5.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi49 – 502KK → AA: ATP-binding site mutant; unable to activate MAPKAPK5. 1 Publication
    Mutagenesisi168 – 1681D → A: Kinase defective mutant, abolishes activity. 2 Publications
    Mutagenesisi186 – 1861S → A or D: Unable to activate MAPKAPK5 promote MAPKAPK5 localization to the cytoplasm. 3 Publications
    Mutagenesisi328 – 3281F → A or Y: Impairs binding to MAPKAPK5. 2 Publications
    Mutagenesisi330 – 3301I → K: Abolishes binding to MAPKAPK5. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 583583Mitogen-activated protein kinase 4PRO_0000186255Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei186 – 1861Phosphoserine; by PAK1, PAK2 and PAK32 Publications

    Post-translational modificationi

    Phosphorylated at Ser-186 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites.2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ6P5G0.
    PRIDEiQ6P5G0.

    PTM databases

    PhosphoSiteiQ6P5G0.

    Expressioni

    Gene expression databases

    BgeeiQ6P5G0.
    CleanExiMM_MAPK4.
    GenevestigatoriQ6P5G0.

    Interactioni

    Subunit structurei

    Homodimer. Heterodimer with ERK3/MAPK6. Interacts with (via FRIEDE motif) MAPKAPK5.4 Publications

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000089462.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6P5G0.
    SMRiQ6P5G0. Positions 13-421.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini20 – 312293Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi186 – 1883SEG motif
    Motifi328 – 3336FRIEDE motif

    Domaini

    The FRIEDE motif is required for docking MAPKAPK5.1 Publication
    In contrast to classical MAPKs, the TXY motif within the activation loop is replaced by the SEG motif, whose phosphorylation activates the MAP kinases.1 Publication

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00550000074298.
    HOGENOMiHOG000233020.
    HOVERGENiHBG104376.
    InParanoidiQ6P5G0.
    KOiK06855.
    OMAiRCQDASE.
    OrthoDBiEOG780RKS.
    PhylomeDBiQ6P5G0.
    TreeFamiTF105098.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR008350. MAPK_ERK3/4.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR01771. ERK3ERK4MAPK.
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6P5G0-1 [UniParc]FASTAAdd to Basket

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    MAEKGDCIAS VYGYDLGGRF IDFQPLGFGV NGLVLSATDS RACRKVAVKK    50
    IVLSDARSMK HALREIKIIR RLDHDNIVKV YEVLGPKGSD LQGELFKFSV 100
    AYIVQEYMET DLACLLEQGT LTEDHAKLFM YQLLRGLKYI HSANVLHRDL 150
    KPANIFISTE DLVLKIGDFG LARIVDQHYS HKGYLSEGLV TKWYRSPRLL 200
    LSPNNYTKAI DMWAAGCILA EMLTGKMLFA GAHELEQMQL ILDTIPVVRE 250
    EDKEELLRVM PSFVSSTWEV KRPLRKLLPD VNSEAIDFLE KILTFNPMDR 300
    LTAEMGLQHP YMSPYSCPED EPTSQHPFRI EDEIDDIVLM AASQSQLSNW 350
    DRYPVSLSSD LEWRPDRCQD ASEVQRDPRA GSTPLAEDVQ VDPRKDSQSS 400
    SERFLEQSHS SMERAFEADY GRSCDYKVGS PSYLDKLLWR DNKPHHYSEP 450
    KLILDLSHWK QAASAPPRAA VAADPVSRED EPASLFLEIA QWVKSTQSGS 500
    ERASPPPDAP EPRLSASPPG HPTPIDGGAS PQFDLDVFIS RALKLCTKPE 550
    DLPENKLGDL NGACISEHPG DLVQTEAFSK ERW 583
    Length:583
    Mass (Da):65,574
    Last modified:July 5, 2004 - v1
    Checksum:i7CE7B2D5359CB52A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC058942 mRNA. Translation: AAH58942.1.
    BC062911 mRNA. Translation: AAH62911.1.
    CCDSiCCDS37856.1.
    RefSeqiNP_766220.2. NM_172632.2.
    XP_006525963.1. XM_006525900.1.
    XP_006525964.1. XM_006525901.1.
    UniGeneiMm.254517.

    Genome annotation databases

    EnsembliENSMUST00000091851; ENSMUSP00000089462; ENSMUSG00000024558.
    GeneIDi225724.
    KEGGimmu:225724.
    UCSCiuc008fpa.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC058942 mRNA. Translation: AAH58942.1 .
    BC062911 mRNA. Translation: AAH62911.1 .
    CCDSi CCDS37856.1.
    RefSeqi NP_766220.2. NM_172632.2.
    XP_006525963.1. XM_006525900.1.
    XP_006525964.1. XM_006525901.1.
    UniGenei Mm.254517.

    3D structure databases

    ProteinModelPortali Q6P5G0.
    SMRi Q6P5G0. Positions 13-421.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000089462.

    PTM databases

    PhosphoSitei Q6P5G0.

    Proteomic databases

    PaxDbi Q6P5G0.
    PRIDEi Q6P5G0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000091851 ; ENSMUSP00000089462 ; ENSMUSG00000024558 .
    GeneIDi 225724.
    KEGGi mmu:225724.
    UCSCi uc008fpa.2. mouse.

    Organism-specific databases

    CTDi 5596.
    MGIi MGI:2444559. Mapk4.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00550000074298.
    HOGENOMi HOG000233020.
    HOVERGENi HBG104376.
    InParanoidi Q6P5G0.
    KOi K06855.
    OMAi RCQDASE.
    OrthoDBi EOG780RKS.
    PhylomeDBi Q6P5G0.
    TreeFami TF105098.

    Miscellaneous databases

    ChiTaRSi MAPK4. mouse.
    NextBioi 377772.
    PROi Q6P5G0.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q6P5G0.
    CleanExi MM_MAPK4.
    Genevestigatori Q6P5G0.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR008350. MAPK_ERK3/4.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR01771. ERK3ERK4MAPK.
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    2. "Characterization of the atypical MAPK ERK4 and its activation of the MAPK-activated protein kinase MK5."
      Kant S., Schumacher S., Singh M.K., Kispert A., Kotlyarov A., Gaestel M.
      J. Biol. Chem. 281:35511-35519(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN PHOSPHORYLATION OF MAPKAPK5, SUBCELLULAR LOCATION, INTERACTION WITH MAPKAPK5 AND MAPK6, SUBUNIT, MUTAGENESIS OF 49-LYS-LYS-50.
    3. "The Ser(186) phospho-acceptor site within ERK4 is essential for its ability to interact with and activate PRAK/MK5."
      Perander M., Aberg E., Johansen B., Dreyer B., Guldvik I.J., Outzen H., Keyse S.M., Seternes O.M.
      Biochem. J. 411:613-622(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MAPKAPK5, PHOSPHORYLATION AT SER-186, MUTAGENESIS OF ASP-168 AND SER-186.
    4. "Activation loop phosphorylation of the atypical MAP kinases ERK3 and ERK4 is required for binding, activation and cytoplasmic relocalization of MK5."
      Deleris P., Rousseau J., Coulombe P., Rodier G., Tanguay P.L., Meloche S.
      J. Cell. Physiol. 217:778-788(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MAPKAPK5, PHOSPHORYLATION AT SER-186, MUTAGENESIS OF SER-186.
    5. "Docking of PRAK/MK5 to the atypical MAPKs ERK3 and ERK4 defines a novel MAPK interaction motif."
      Aberg E., Torgersen K.M., Johansen B., Keyse S.M., Perander M., Seternes O.M.
      J. Biol. Chem. 284:19392-19401(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MAPKAPK5, DOMAIN FRIEDE MOTIF, MUTAGENESIS OF PHE-328 AND ILE-330.

    Entry informationi

    Entry nameiMK04_MOUSE
    AccessioniPrimary (citable) accession number: Q6P5G0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2004
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3