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Q6P5E8

- DGKQ_MOUSE

UniProt

Q6P5E8 - DGKQ_MOUSE

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Protein

Diacylglycerol kinase theta

Gene

Dgkq

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor (By similarity). May be involved in cAMP-induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity (By similarity).By similarity

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Enzyme regulationi

Inactivated by binding to RHOA. Not inhibited by phosphatidylserine (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri54 – 10249Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri115 – 16248Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri177 – 22852Phorbol-ester/DAG-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. diacylglycerol kinase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. NAD+ kinase activity Source: InterPro

GO - Biological processi

  1. cAMP-mediated signaling Source: Ensembl
  2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
  3. protein kinase C signaling Source: Ensembl
  4. regulation of transcription from RNA polymerase II promoter Source: Ensembl
  5. response to ATP Source: Ensembl
  6. thrombin receptor signaling pathway Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_219232. Effects of PIP2 hydrolysis.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase theta (EC:2.7.1.107)
Short name:
DAG kinase theta
Alternative name(s):
Diglyceride kinase theta
Short name:
DGK-theta
Gene namesi
Name:Dgkq
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:102918. Dgkq.

Subcellular locationi

Cytoplasm By similarity. Cell membrane By similarity. Cytoplasmcytoskeleton By similarity. Nucleus By similarity. Nucleus speckle By similarity
Note: Translocates to the nucleus in response to thrombin stimulation. Translocates to the plasma membrane in response to steroid hormone receptor stimulation. Translocation to the plasma membrane is dependent on G-protein coupled receptor stimulation and subsequent activation of PRKCE and probably PRKCH.By similarity

GO - Cellular componenti

  1. cytoskeleton Source: UniProtKB-KW
  2. cytosol Source: Ensembl
  3. nuclear speck Source: Ensembl
  4. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 934934Diacylglycerol kinase thetaPRO_0000381763Add
BLAST

Post-translational modificationi

Phosphorylated by PRKCE and PRKCH in vitro.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6P5E8.
PRIDEiQ6P5E8.

PTM databases

PhosphoSiteiQ6P5E8.

Expressioni

Gene expression databases

BgeeiQ6P5E8.
ExpressionAtlasiQ6P5E8. baseline.
GenevestigatoriQ6P5E8.

Interactioni

Subunit structurei

Interacts with RHOA (constitutively activated, GTP-bound); the interaction inhibits DGKQ. Interacts with PRKCE. Interacts with PRKCH. Interacts with PLCB1. Interacts with NR5A1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000057859.

Structurei

3D structure databases

ProteinModelPortaliQ6P5E8.
SMRiQ6P5E8. Positions 50-102, 178-229.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini387 – 486100Ras-associatingPROSITE-ProRule annotationAdd
BLAST
Domaini576 – 713138DAGKcPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation
Contains 3 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation
Contains 1 Ras-associating domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri54 – 10249Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri115 – 16248Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri177 – 22852Phorbol-ester/DAG-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG47311.
GeneTreeiENSGT00760000119050.
HOGENOMiHOG000007900.
InParanoidiQ6P5E8.
KOiK00901.
OMAiSDFIWGL.
OrthoDBiEOG71VSS6.
PhylomeDBiQ6P5E8.
TreeFamiTF312817.

Family and domain databases

InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR020454. DAG/PE-bd.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR002219. PE/DAG-bd.
IPR000159. Ras-assoc.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF00788. RA. 2 hits.
[Graphical view]
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 3 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00314. RA. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
SSF54236. SSF54236. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
PS50200. RA. 1 hit.
PS00479. ZF_DAG_PE_1. 3 hits.
PS50081. ZF_DAG_PE_2. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6P5E8-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAAEPGAR TWPGSGSPRL GSPAGSPVLG ISGRTRPGSG PERTSRAIGS
60 70 80 90 100
AAPGHSFRKV TLTKPTFCHL CSDFIWGLAG FLCDVCNFMS HEKCLKQVKT
110 120 130 140 150
PCTSIAPSLV RVPVAHCFGS LGLYKRKFCV VCRKSLEVPA FRCEVCELHV
160 170 180 190 200
HPDCVPFACS DCRQCHQDGQ QDYDTYHHHW REGNLPSGAR CEVCRKTCGS
210 220 230 240 250
SDVLAGVRCE WCGVQAHSVC STALAPECTF GRLRSMVLPP SCVRLLSRNF
260 270 280 290 300
SKMHCFRIPE TMVLELGDGD DGVDGSAAIG TGREVLTATE STKQTLKIFD
310 320 330 340 350
GNDSMRKNQF RLVTVSRLAR NEEVMEAALR AYYISEDPKD FQLQALPLSG
360 370 380 390 400
NAQALGKAGT TEEEASKGSC PRDSVPEAWV IRSLPRTQEI LKIYPGWLKV
410 420 430 440 450
GVAYVSIRVN SQSTARSVVQ EVLPLFGQQV EDKERFQLIE VLMSSRQVQR
460 470 480 490 500
TVLADEEPLL DRLWDIRQTS VRQVSQTRFY VAETRATAPR VSLFVGGLPP
510 520 530 540 550
GLSPQDYSNL LHEAMATKAA VVSVSHVYSL QGAVILDVTC FAEAERLYML
560 570 580 590 600
ARDTAVHGRP LTALVLPDVL HTKLPPDCCP LLVFVNPKSG GLKGRELLCS
610 620 630 640 650
FRKLLNPHQV FELTNGGPLP GFHLFSQVPS FRVLVCGGDG TVGWVLAALE
660 670 680 690 700
ETRRHLACPE PSVAILPLGT GNDLGRVLRW GAGYSGEDPF SVLVSVDEAD
710 720 730 740 750
AVLMDRWTIL LDAHEIDSTE NNVVETEPPK IVQMNNYCGI GIDAELSLDF
760 770 780 790 800
HQAREEEPGK FTSRFHNKGV YVRVGLQKIS HSRSLHKEIR LQVEQQEVEL
810 820 830 840 850
PSIEGLIFIN IPSWGSGADL WGSDNDSRFE KPRIDDGLLE VVGVTGVVHM
860 870 880 890 900
GQVQGGLRSG IRIAQGSYFR VTLLKATPVQ VDGEPWVQAP GHMIISATAP
910 920 930
KVHMLRKAKQ KPRKAGANRD TRVDTLPAPE GNPL
Length:934
Mass (Da):102,254
Last modified:July 5, 2004 - v1
Checksum:iC7FA90936443818E
GO
Isoform 2 (identifier: Q6P5E8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-548: Missing.

Note: No experimental confirmation available.

Show »
Length:386
Mass (Da):42,209
Checksum:i8430D1C19674B841
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 548548Missing in isoform 2. 1 PublicationVSP_037832Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK134745 mRNA. Translation: BAE22264.1.
BC062929 mRNA. Translation: AAH62929.1.
CCDSiCCDS19515.1. [Q6P5E8-1]
RefSeqiNP_950176.1. NM_199011.1. [Q6P5E8-1]
UniGeneiMm.260921.

Genome annotation databases

EnsembliENSMUST00000053913; ENSMUSP00000057859; ENSMUSG00000004815. [Q6P5E8-1]
GeneIDi110524.
KEGGimmu:110524.
UCSCiuc008yot.1. mouse. [Q6P5E8-1]
uc012eau.1. mouse. [Q6P5E8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK134745 mRNA. Translation: BAE22264.1 .
BC062929 mRNA. Translation: AAH62929.1 .
CCDSi CCDS19515.1. [Q6P5E8-1 ]
RefSeqi NP_950176.1. NM_199011.1. [Q6P5E8-1 ]
UniGenei Mm.260921.

3D structure databases

ProteinModelPortali Q6P5E8.
SMRi Q6P5E8. Positions 50-102, 178-229.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000057859.

PTM databases

PhosphoSitei Q6P5E8.

Proteomic databases

PaxDbi Q6P5E8.
PRIDEi Q6P5E8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000053913 ; ENSMUSP00000057859 ; ENSMUSG00000004815 . [Q6P5E8-1 ]
GeneIDi 110524.
KEGGi mmu:110524.
UCSCi uc008yot.1. mouse. [Q6P5E8-1 ]
uc012eau.1. mouse. [Q6P5E8-2 ]

Organism-specific databases

CTDi 1609.
MGIi MGI:102918. Dgkq.

Phylogenomic databases

eggNOGi NOG47311.
GeneTreei ENSGT00760000119050.
HOGENOMi HOG000007900.
InParanoidi Q6P5E8.
KOi K00901.
OMAi SDFIWGL.
OrthoDBi EOG71VSS6.
PhylomeDBi Q6P5E8.
TreeFami TF312817.

Enzyme and pathway databases

Reactomei REACT_219232. Effects of PIP2 hydrolysis.

Miscellaneous databases

NextBioi 364149.
PROi Q6P5E8.
SOURCEi Search...

Gene expression databases

Bgeei Q6P5E8.
ExpressionAtlasi Q6P5E8. baseline.
Genevestigatori Q6P5E8.

Family and domain databases

InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR020454. DAG/PE-bd.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR002219. PE/DAG-bd.
IPR000159. Ras-assoc.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
Pfami PF00130. C1_1. 2 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF00788. RA. 2 hits.
[Graphical view ]
PRINTSi PR00008. DAGPEDOMAIN.
SMARTi SM00109. C1. 3 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00314. RA. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 1 hit.
SSF54236. SSF54236. 2 hits.
PROSITEi PS50146. DAGK. 1 hit.
PS50200. RA. 1 hit.
PS00479. ZF_DAG_PE_1. 3 hits.
PS50081. ZF_DAG_PE_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Medulla oblongata.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiDGKQ_MOUSE
AccessioniPrimary (citable) accession number: Q6P5E8
Secondary accession number(s): Q3UYE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3