Q6P5E8 (DGKQ_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacylglycerol kinase theta
Short name=DAG kinase theta
EC=2.7.1.107

Alternative name(s):
Diglyceride kinase theta
Short name=DGK-theta

Gene names

Name:

Dgkq

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	934 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor By similarity. May be involved in cAMP-induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity By similarity.
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Enzyme regulation	Inactivated by binding to RHOA. Not inhibited by phosphatidylserine By similarity.
Subunit structure	Interacts with RHOA (constitutively activated, GTP-bound); the interaction inhibits DGKQ. Interacts with PRKCE. Interacts with PRKCH. Interacts with PLCB1. Interacts with NR5A1 By similarity.
Subcellular location	Cytoplasm By similarity. Cell membrane By similarity. Cytoplasm › cytoskeleton By similarity. Nucleus By similarity. Nucleus speckle By similarity. Note: Translocates to the nucleus in response to thrombin stimulation By similarity. Translocates to the plasma membrane in response to steroid hormone receptor stimulation By similarity. Translocation to the plasma membrane is dependent on G-protein coupled receptor stimulation and subsequent activation of PRKCE and probably PRKCH By similarity.
Post-translational modification	Phosphorylated by PRKCE and PRKCH in vitro By similarity.
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 3 phorbol-ester/DAG-type zinc fingers. Contains 1 Ras-associating domain.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Cytoskeleton Membrane Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cAMP-mediated signaling Inferred from electronic annotation. Source: Compara protein kinase C signaling cascade Inferred from electronic annotation. Source: Compara protein kinase C-activating G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: InterPro response to ATP Inferred from electronic annotation. Source: Compara thrombin receptor signaling pathway Inferred from electronic annotation. Source: Compara
Cellular_component	cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Inferred from electronic annotation. Source: Compara nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6P5E8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6P5E8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-548: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 934	934	Diacylglycerol kinase theta		PRO_0000381763
Regions
	Domain	387 – 486	100	Ras-associating
	Domain	576 – 713	138	DAGKc
	Zinc finger	54 – 102	49	Phorbol-ester/DAG-type 1
	Zinc finger	115 – 162	48	Phorbol-ester/DAG-type 2
	Zinc finger	177 – 228	52	Phorbol-ester/DAG-type 3
Amino acid modifications
	Modified residue	22	1	Phosphoserine Ref.3
	Modified residue	26	1	Phosphoserine Ref.3
Natural variations
	Alternative sequence	1 – 548	548	Missing in isoform 2.		VSP_037832

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C7FA90936443818E	FASTA	934	102,254
	10 20 30 40 50 60 MAAAAEPGAR TWPGSGSPRL GSPAGSPVLG ISGRTRPGSG PERTSRAIGS AAPGHSFRKV 70 80 90 100 110 120 TLTKPTFCHL CSDFIWGLAG FLCDVCNFMS HEKCLKQVKT PCTSIAPSLV RVPVAHCFGS 130 140 150 160 170 180 LGLYKRKFCV VCRKSLEVPA FRCEVCELHV HPDCVPFACS DCRQCHQDGQ QDYDTYHHHW 190 200 210 220 230 240 REGNLPSGAR CEVCRKTCGS SDVLAGVRCE WCGVQAHSVC STALAPECTF GRLRSMVLPP 250 260 270 280 290 300 SCVRLLSRNF SKMHCFRIPE TMVLELGDGD DGVDGSAAIG TGREVLTATE STKQTLKIFD 310 320 330 340 350 360 GNDSMRKNQF RLVTVSRLAR NEEVMEAALR AYYISEDPKD FQLQALPLSG NAQALGKAGT 370 380 390 400 410 420 TEEEASKGSC PRDSVPEAWV IRSLPRTQEI LKIYPGWLKV GVAYVSIRVN SQSTARSVVQ 430 440 450 460 470 480 EVLPLFGQQV EDKERFQLIE VLMSSRQVQR TVLADEEPLL DRLWDIRQTS VRQVSQTRFY 490 500 510 520 530 540 VAETRATAPR VSLFVGGLPP GLSPQDYSNL LHEAMATKAA VVSVSHVYSL QGAVILDVTC 550 560 570 580 590 600 FAEAERLYML ARDTAVHGRP LTALVLPDVL HTKLPPDCCP LLVFVNPKSG GLKGRELLCS 610 620 630 640 650 660 FRKLLNPHQV FELTNGGPLP GFHLFSQVPS FRVLVCGGDG TVGWVLAALE ETRRHLACPE 670 680 690 700 710 720 PSVAILPLGT GNDLGRVLRW GAGYSGEDPF SVLVSVDEAD AVLMDRWTIL LDAHEIDSTE 730 740 750 760 770 780 NNVVETEPPK IVQMNNYCGI GIDAELSLDF HQAREEEPGK FTSRFHNKGV YVRVGLQKIS 790 800 810 820 830 840 HSRSLHKEIR LQVEQQEVEL PSIEGLIFIN IPSWGSGADL WGSDNDSRFE KPRIDDGLLE 850 860 870 880 890 900 VVGVTGVVHM GQVQGGLRSG IRIAQGSYFR VTLLKATPVQ VDGEPWVQAP GHMIISATAP 910 920 930 KVHMLRKAKQ KPRKAGANRD TRVDTLPAPE GNPL
	Isoform 2 [UniParc]. Checksum: 8430D1C19674B841	FASTA	386	42,209
	10 20 30 40 50 60 MLARDTAVHG RPLTALVLPD VLHTKLPPDC CPLLVFVNPK SGGLKGRELL CSFRKLLNPH 70 80 90 100 110 120 QVFELTNGGP LPGFHLFSQV PSFRVLVCGG DGTVGWVLAA LEETRRHLAC PEPSVAILPL 130 140 150 160 170 180 GTGNDLGRVL RWGAGYSGED PFSVLVSVDE ADAVLMDRWT ILLDAHEIDS TENNVVETEP 190 200 210 220 230 240 PKIVQMNNYC GIGIDAELSL DFHQAREEEP GKFTSRFHNK GVYVRVGLQK ISHSRSLHKE 250 260 270 280 290 300 IRLQVEQQEV ELPSIEGLIF INIPSWGSGA DLWGSDNDSR FEKPRIDDGL LEVVGVTGVV 310 320 330 340 350 360 HMGQVQGGLR SGIRIAQGSY FRVTLLKATP VQVDGEPWVQ APGHMIISAT APKVHMLRKA 370 380 KQKPRKAGAN RDTRVDTLPA PEGNPL

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Medulla oblongata.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
[3]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22 AND SER-26, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK134745 mRNA. Translation: BAE22264.1. BC062929 mRNA. Translation: AAH62929.1.
IPI	IPI00396685. IPI00944172.
RefSeq	NP_950176.1. NM_199011.1.
UniGene	Mm.260921.
3D structure databases
HSSP	HSSP built from PDB template 1R79 based on UniProtKB Q16760.
ProteinModelPortal	Q6P5E8.
SMR	Q6P5E8. Positions 50-102, 116-162, 178-229.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000057859.
PTM databases
PhosphoSite	Q6P5E8.
Proteomic databases
PaxDb	Q6P5E8.
PRIDE	Q6P5E8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000053913; ENSMUSP00000057859; ENSMUSG00000004815.
GeneID	110524.
KEGG	mmu:110524.
UCSC	uc008yot.1. mouse. uc012eau.1. mouse.
Organism-specific databases
CTD	1609.
MGI	MGI:102918. Dgkq.
Phylogenomic databases
eggNOG	NOG47311.
GeneTree	ENSGT00660000095381.
HOGENOM	HOG000007900.
InParanoid	Q6P5E8.
KO	K00901.
OMA	TKPTFCH.
OrthoDB	EOG49KFPV.
Gene expression databases
ArrayExpress	Q6P5E8.
Bgee	Q6P5E8.
Genevestigator	Q6P5E8.
Family and domain databases
InterPro	IPR020454. DAG/PE-bd. IPR000756. Diacylglycerol_kin_accessory. IPR001206. Diacylglycerol_kinase_cat_dom. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. IPR000159. Ras-assoc. [Graphical view]
Pfam	PF00130. C1_1. 2 hits. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. PF00788. RA. 1 hit. [Graphical view]
PRINTS	PR00008. DAGPEDOMAIN.
SMART	SM00109. C1. 3 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. SM00314. RA. 1 hit. [Graphical view]
PROSITE	PS50146. DAGK. 1 hit. PS50200. RA. 1 hit. PS00479. ZF_DAG_PE_1. 3 hits. PS50081. ZF_DAG_PE_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	364149.
SOURCE	Search...

Entry information

Entry name

DGKQ_MOUSE

Accession

Primary (citable) accession number: Q6P5E8
Secondary accession number(s): Q3UYE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 1, 2009
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program