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Q6P5E8

- DGKQ_MOUSE

UniProt

Q6P5E8 - DGKQ_MOUSE

Protein

Diacylglycerol kinase theta

Gene

Dgkq

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor By similarity. May be involved in cAMP-induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity By similarity.By similarity

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

    Enzyme regulationi

    Inactivated by binding to RHOA. Not inhibited by phosphatidylserine By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri54 – 10249Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri115 – 16248Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri177 – 22852Phorbol-ester/DAG-type 3PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. diacylglycerol kinase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. NAD+ kinase activity Source: InterPro

    GO - Biological processi

    1. cAMP-mediated signaling Source: Ensembl
    2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
    3. protein kinase C signaling Source: Ensembl
    4. response to ATP Source: Ensembl
    5. thrombin receptor signaling pathway Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_219232. Effects of PIP2 hydrolysis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase theta (EC:2.7.1.107)
    Short name:
    DAG kinase theta
    Alternative name(s):
    Diglyceride kinase theta
    Short name:
    DGK-theta
    Gene namesi
    Name:Dgkq
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:102918. Dgkq.

    Subcellular locationi

    Cytoplasm By similarity. Cell membrane By similarity. Cytoplasmcytoskeleton By similarity. Nucleus By similarity. Nucleus speckle By similarity
    Note: Translocates to the nucleus in response to thrombin stimulation. Translocates to the plasma membrane in response to steroid hormone receptor stimulation. Translocation to the plasma membrane is dependent on G-protein coupled receptor stimulation and subsequent activation of PRKCE and probably PRKCH.By similarity

    GO - Cellular componenti

    1. cytoskeleton Source: UniProtKB-SubCell
    2. cytosol Source: Ensembl
    3. nuclear speck Source: UniProtKB-SubCell
    4. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 934934Diacylglycerol kinase thetaPRO_0000381763Add
    BLAST

    Post-translational modificationi

    Phosphorylated by PRKCE and PRKCH in vitro.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ6P5E8.
    PRIDEiQ6P5E8.

    PTM databases

    PhosphoSiteiQ6P5E8.

    Expressioni

    Gene expression databases

    BgeeiQ6P5E8.
    GenevestigatoriQ6P5E8.

    Interactioni

    Subunit structurei

    Interacts with RHOA (constitutively activated, GTP-bound); the interaction inhibits DGKQ. Interacts with PRKCE. Interacts with PRKCH. Interacts with PLCB1. Interacts with NR5A1 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000057859.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6P5E8.
    SMRiQ6P5E8. Positions 50-102, 178-229.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini387 – 486100Ras-associatingPROSITE-ProRule annotationAdd
    BLAST
    Domaini576 – 713138DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DAGKc domain.PROSITE-ProRule annotation
    Contains 3 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation
    Contains 1 Ras-associating domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri54 – 10249Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri115 – 16248Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri177 – 22852Phorbol-ester/DAG-type 3PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG47311.
    GeneTreeiENSGT00750000117493.
    HOGENOMiHOG000007900.
    InParanoidiQ6P5E8.
    KOiK00901.
    OMAiSDFIWGL.
    OrthoDBiEOG71VSS6.
    PhylomeDBiQ6P5E8.
    TreeFamiTF312817.

    Family and domain databases

    InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR020454. DAG/PE-bd.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000159. Ras-assoc.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PfamiPF00130. C1_1. 2 hits.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    PF00788. RA. 2 hits.
    [Graphical view]
    PRINTSiPR00008. DAGPEDOMAIN.
    SMARTiSM00109. C1. 3 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00314. RA. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.
    SSF54236. SSF54236. 2 hits.
    PROSITEiPS50146. DAGK. 1 hit.
    PS50200. RA. 1 hit.
    PS00479. ZF_DAG_PE_1. 3 hits.
    PS50081. ZF_DAG_PE_2. 3 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6P5E8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAAAEPGAR TWPGSGSPRL GSPAGSPVLG ISGRTRPGSG PERTSRAIGS    50
    AAPGHSFRKV TLTKPTFCHL CSDFIWGLAG FLCDVCNFMS HEKCLKQVKT 100
    PCTSIAPSLV RVPVAHCFGS LGLYKRKFCV VCRKSLEVPA FRCEVCELHV 150
    HPDCVPFACS DCRQCHQDGQ QDYDTYHHHW REGNLPSGAR CEVCRKTCGS 200
    SDVLAGVRCE WCGVQAHSVC STALAPECTF GRLRSMVLPP SCVRLLSRNF 250
    SKMHCFRIPE TMVLELGDGD DGVDGSAAIG TGREVLTATE STKQTLKIFD 300
    GNDSMRKNQF RLVTVSRLAR NEEVMEAALR AYYISEDPKD FQLQALPLSG 350
    NAQALGKAGT TEEEASKGSC PRDSVPEAWV IRSLPRTQEI LKIYPGWLKV 400
    GVAYVSIRVN SQSTARSVVQ EVLPLFGQQV EDKERFQLIE VLMSSRQVQR 450
    TVLADEEPLL DRLWDIRQTS VRQVSQTRFY VAETRATAPR VSLFVGGLPP 500
    GLSPQDYSNL LHEAMATKAA VVSVSHVYSL QGAVILDVTC FAEAERLYML 550
    ARDTAVHGRP LTALVLPDVL HTKLPPDCCP LLVFVNPKSG GLKGRELLCS 600
    FRKLLNPHQV FELTNGGPLP GFHLFSQVPS FRVLVCGGDG TVGWVLAALE 650
    ETRRHLACPE PSVAILPLGT GNDLGRVLRW GAGYSGEDPF SVLVSVDEAD 700
    AVLMDRWTIL LDAHEIDSTE NNVVETEPPK IVQMNNYCGI GIDAELSLDF 750
    HQAREEEPGK FTSRFHNKGV YVRVGLQKIS HSRSLHKEIR LQVEQQEVEL 800
    PSIEGLIFIN IPSWGSGADL WGSDNDSRFE KPRIDDGLLE VVGVTGVVHM 850
    GQVQGGLRSG IRIAQGSYFR VTLLKATPVQ VDGEPWVQAP GHMIISATAP 900
    KVHMLRKAKQ KPRKAGANRD TRVDTLPAPE GNPL 934
    Length:934
    Mass (Da):102,254
    Last modified:July 5, 2004 - v1
    Checksum:iC7FA90936443818E
    GO
    Isoform 2 (identifier: Q6P5E8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-548: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:386
    Mass (Da):42,209
    Checksum:i8430D1C19674B841
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 548548Missing in isoform 2. 1 PublicationVSP_037832Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK134745 mRNA. Translation: BAE22264.1.
    BC062929 mRNA. Translation: AAH62929.1.
    CCDSiCCDS19515.1. [Q6P5E8-1]
    RefSeqiNP_950176.1. NM_199011.1. [Q6P5E8-1]
    UniGeneiMm.260921.

    Genome annotation databases

    EnsembliENSMUST00000053913; ENSMUSP00000057859; ENSMUSG00000004815. [Q6P5E8-1]
    GeneIDi110524.
    KEGGimmu:110524.
    UCSCiuc008yot.1. mouse. [Q6P5E8-1]
    uc012eau.1. mouse. [Q6P5E8-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK134745 mRNA. Translation: BAE22264.1 .
    BC062929 mRNA. Translation: AAH62929.1 .
    CCDSi CCDS19515.1. [Q6P5E8-1 ]
    RefSeqi NP_950176.1. NM_199011.1. [Q6P5E8-1 ]
    UniGenei Mm.260921.

    3D structure databases

    ProteinModelPortali Q6P5E8.
    SMRi Q6P5E8. Positions 50-102, 178-229.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000057859.

    PTM databases

    PhosphoSitei Q6P5E8.

    Proteomic databases

    PaxDbi Q6P5E8.
    PRIDEi Q6P5E8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000053913 ; ENSMUSP00000057859 ; ENSMUSG00000004815 . [Q6P5E8-1 ]
    GeneIDi 110524.
    KEGGi mmu:110524.
    UCSCi uc008yot.1. mouse. [Q6P5E8-1 ]
    uc012eau.1. mouse. [Q6P5E8-2 ]

    Organism-specific databases

    CTDi 1609.
    MGIi MGI:102918. Dgkq.

    Phylogenomic databases

    eggNOGi NOG47311.
    GeneTreei ENSGT00750000117493.
    HOGENOMi HOG000007900.
    InParanoidi Q6P5E8.
    KOi K00901.
    OMAi SDFIWGL.
    OrthoDBi EOG71VSS6.
    PhylomeDBi Q6P5E8.
    TreeFami TF312817.

    Enzyme and pathway databases

    Reactomei REACT_219232. Effects of PIP2 hydrolysis.

    Miscellaneous databases

    NextBioi 364149.
    PROi Q6P5E8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q6P5E8.
    Genevestigatori Q6P5E8.

    Family and domain databases

    InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR020454. DAG/PE-bd.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000159. Ras-assoc.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    Pfami PF00130. C1_1. 2 hits.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    PF00788. RA. 2 hits.
    [Graphical view ]
    PRINTSi PR00008. DAGPEDOMAIN.
    SMARTi SM00109. C1. 3 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00314. RA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 1 hit.
    SSF54236. SSF54236. 2 hits.
    PROSITEi PS50146. DAGK. 1 hit.
    PS50200. RA. 1 hit.
    PS00479. ZF_DAG_PE_1. 3 hits.
    PS50081. ZF_DAG_PE_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Medulla oblongata.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiDGKQ_MOUSE
    AccessioniPrimary (citable) accession number: Q6P5E8
    Secondary accession number(s): Q3UYE8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 1, 2009
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3