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Protein

ADP-ribosylation factor-binding protein GGA2

Gene

Gga2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-binding protein GGA2
Alternative name(s):
Gamma-adaptin-related protein 2
Golgi-localized, gamma ear-containing, ARF-binding protein 2
Gene namesi
Name:Gga2
Synonyms:Kiaa1080
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1921355. Gga2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 603603ADP-ribosylation factor-binding protein GGA2PRO_0000212683Add
BLAST

Post-translational modificationi

Ubiquitinated.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

EPDiQ6P5E6.
MaxQBiQ6P5E6.
PaxDbiQ6P5E6.
PeptideAtlasiQ6P5E6.
PRIDEiQ6P5E6.

PTM databases

iPTMnetiQ6P5E6.
PhosphoSiteiQ6P5E6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030872.
GenevisibleiQ6P5E6. MM.

Interactioni

Subunit structurei

Monomer. Interacts with NECAP1, TSG101, UBC and AFTPH/aftiphilin. Interacts with GGA1 and GGA3. Binds to clathrin and activated ARFs. Binds RABEP1 and RABGEF1. Interacts with the type-I membrane proteins SORT1, SORL1, LRP3, M6PR/CD-MPR, IGF2R/CI-MPR and BACE1. Binds CCDC91, P200, SYNRG, EPN4 and NECAP2 (By similarity). Interacts with CNST.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CnstQ8CBC4-32EBI-2616239,EBI-2615407

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ6P5E6. 3 interactions.
MINTiMINT-1342264.
STRINGi10090.ENSMUSP00000033160.

Structurei

3D structure databases

ProteinModelPortaliQ6P5E6.
SMRiQ6P5E6. Positions 28-170, 190-318, 461-595.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 166131VHSPROSITE-ProRule annotationAdd
BLAST
Domaini190 – 317128GATPROSITE-ProRule annotationAdd
BLAST
Domaini474 – 595122GAEPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni318 – 473156Unstructured hingeAdd
BLAST

Domaini

The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).By similarity
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis (By similarity).By similarity
The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.By similarity
The GAE domain binds accessory proteins regulating GGAs function.By similarity

Sequence similaritiesi

Belongs to the GGA protein family.Curated
Contains 1 GAE domain.PROSITE-ProRule annotation
Contains 1 GAT domain.PROSITE-ProRule annotation
Contains 1 VHS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1087. Eukaryota.
ENOG410Y26G. LUCA.
GeneTreeiENSGT00700000104396.
HOGENOMiHOG000231169.
HOVERGENiHBG015945.
InParanoidiQ6P5E6.
KOiK12404.
OMAiKVAGQNC.
OrthoDBiEOG091G0BQK.
PhylomeDBiQ6P5E6.
TreeFamiTF318574.

Family and domain databases

Gene3Di1.20.58.160. 1 hit.
1.25.40.90. 1 hit.
2.60.40.1230. 1 hit.
InterProiIPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR008942. ENTH_VHS.
IPR004152. GAT_dom.
IPR002014. VHS_dom.
[Graphical view]
PfamiPF02883. Alpha_adaptinC2. 1 hit.
PF03127. GAT. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
SMARTiSM00809. Alpha_adaptinC2. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEiPS50180. GAE. 1 hit.
PS50909. GAT. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6P5E6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAATAVAAGT GSPAGTESAE GGPGAAAALE LWLNKATDPS MAEQDWSAIQ
60 70 80 90 100
KFCEQVNTDP SGPTHAPWLL AHKIQSPQEK EALYALTVLE ICMNHCGEKF
110 120 130 140 150
HSEVAKFRFL NELIKVLSPK YLGAWATEKV KGRVIEILFS WTVWFPEDIK
160 170 180 190 200
IRDAYQMLKK QGIIKQDPKL PMDKILPPPS PWPKSIFDAD EEKSKLLTRL
210 220 230 240 250
LKSNHPEDLQ AANRLIKNLV KEEQEKSEKV SRRVSAVEEV RSHVRVLREM
260 270 280 290 300
LSMYRRPGHA LPDQQALQVV YERCEKLRPT LFRLASDTTD DDDALAEILQ
310 320 330 340 350
ANDLLTQGVR LYKQVVEGRV SAGNAVPAAV GAIPAPRAFP NPEPCGLNCP
360 370 380 390 400
LIDLETPSLL HQDLAALGIN DVPTRNQVVI PSCCNDKKQP GAITLMGGGI
410 420 430 440 450
QSLSADRNLL DLFSPQPSPG LNYVPQKSIP KEVPPGTKAS PGWSWEAGPL
460 470 480 490 500
ASSTASQNTP LAHVFVPLES VKPSSLPPIV VYDRNGFRIL LHFSQTGAPG
510 520 530 540 550
HPDVKVLLLT MMSTATQPVW DVMFQVAVPK SMRVKLQPAS SSKLPAFSPL
560 570 580 590 600
MPPAVISQTL LLDNPHKEPI RLRYKLTFNQ GGQPFSEVGE VKDFPDLAVL

STA
Length:603
Mass (Da):66,049
Last modified:July 5, 2004 - v1
Checksum:i0EFCB3905074982C
GO
Isoform 2 (identifier: Q6P5E6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-249: Missing.

Show »
Length:354
Mass (Da):38,178
Checksum:iE54F3C332CD2C392
GO

Sequence cautioni

The sequence BAC28769 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAC98090 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti228 – 2281E → G in BAB23426 (PubMed:16141072).Curated
Sequence conflicti499 – 4991P → A in BAB23426 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 249249Missing in isoform 2. 1 PublicationVSP_013255Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004632 mRNA. Translation: BAB23426.1.
AK034612 mRNA. Translation: BAC28769.1. Different initiation.
AK129280 mRNA. Translation: BAC98090.1. Different initiation.
AK143691 mRNA. Translation: BAE25501.1.
AK154904 mRNA. Translation: BAE32915.1.
BC057377 mRNA. Translation: AAH57377.1.
BC062933 mRNA. Translation: AAH62933.1.
CCDSiCCDS40116.1. [Q6P5E6-1]
RefSeqiNP_083034.1. NM_028758.2. [Q6P5E6-1]
UniGeneiMm.29619.

Genome annotation databases

EnsembliENSMUST00000033160; ENSMUSP00000033160; ENSMUSG00000030872. [Q6P5E6-1]
ENSMUST00000124566; ENSMUSP00000115581; ENSMUSG00000030872. [Q6P5E6-1]
GeneIDi74105.
KEGGimmu:74105.
UCSCiuc009joa.1. mouse. [Q6P5E6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004632 mRNA. Translation: BAB23426.1.
AK034612 mRNA. Translation: BAC28769.1. Different initiation.
AK129280 mRNA. Translation: BAC98090.1. Different initiation.
AK143691 mRNA. Translation: BAE25501.1.
AK154904 mRNA. Translation: BAE32915.1.
BC057377 mRNA. Translation: AAH57377.1.
BC062933 mRNA. Translation: AAH62933.1.
CCDSiCCDS40116.1. [Q6P5E6-1]
RefSeqiNP_083034.1. NM_028758.2. [Q6P5E6-1]
UniGeneiMm.29619.

3D structure databases

ProteinModelPortaliQ6P5E6.
SMRiQ6P5E6. Positions 28-170, 190-318, 461-595.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6P5E6. 3 interactions.
MINTiMINT-1342264.
STRINGi10090.ENSMUSP00000033160.

PTM databases

iPTMnetiQ6P5E6.
PhosphoSiteiQ6P5E6.

Proteomic databases

EPDiQ6P5E6.
MaxQBiQ6P5E6.
PaxDbiQ6P5E6.
PeptideAtlasiQ6P5E6.
PRIDEiQ6P5E6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033160; ENSMUSP00000033160; ENSMUSG00000030872. [Q6P5E6-1]
ENSMUST00000124566; ENSMUSP00000115581; ENSMUSG00000030872. [Q6P5E6-1]
GeneIDi74105.
KEGGimmu:74105.
UCSCiuc009joa.1. mouse. [Q6P5E6-1]

Organism-specific databases

CTDi23062.
MGIiMGI:1921355. Gga2.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1087. Eukaryota.
ENOG410Y26G. LUCA.
GeneTreeiENSGT00700000104396.
HOGENOMiHOG000231169.
HOVERGENiHBG015945.
InParanoidiQ6P5E6.
KOiK12404.
OMAiKVAGQNC.
OrthoDBiEOG091G0BQK.
PhylomeDBiQ6P5E6.
TreeFamiTF318574.

Miscellaneous databases

PROiQ6P5E6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030872.
GenevisibleiQ6P5E6. MM.

Family and domain databases

Gene3Di1.20.58.160. 1 hit.
1.25.40.90. 1 hit.
2.60.40.1230. 1 hit.
InterProiIPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR008942. ENTH_VHS.
IPR004152. GAT_dom.
IPR002014. VHS_dom.
[Graphical view]
PfamiPF02883. Alpha_adaptinC2. 1 hit.
PF03127. GAT. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
SMARTiSM00809. Alpha_adaptinC2. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEiPS50180. GAE. 1 hit.
PS50909. GAT. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGGA2_MOUSE
AccessioniPrimary (citable) accession number: Q6P5E6
Secondary accession number(s): Q3U374
, Q6PFX3, Q6ZPY8, Q8BM76, Q9DC15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.