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Protein

Single-strand selective monofunctional uracil DNA glycosylase

Gene

Smug1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei86 – 861Substrate; via amide nitrogenBy similarity
Binding sitei100 – 1001Substrate; via amide nitrogenBy similarity
Binding sitei165 – 1651SubstrateBy similarity
Binding sitei241 – 2411SubstrateBy similarity

GO - Molecular functioni

  • DNA N-glycosylase activity Source: HGNC
  • oxidized base lesion DNA N-glycosylase activity Source: MGI
  • oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity Source: Ensembl
  • single-stranded DNA binding Source: MGI
  • single-strand selective uracil DNA N-glycosylase activity Source: HGNC
  • uracil DNA N-glycosylase activity Source: HGNC

GO - Biological processi

  • base-excision repair Source: HGNC
  • DNA repair Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BRENDAi3.2.2.27. 3474.
ReactomeiR-MMU-110329. Cleavage of the damaged pyrimidine.
R-MMU-110357. Displacement of DNA glycosylase by APEX1.

Names & Taxonomyi

Protein namesi
Recommended name:
Single-strand selective monofunctional uracil DNA glycosylase (EC:3.2.2.-)
Gene namesi
Name:Smug1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1918976. Smug1.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

  • nucleolus Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 279279Single-strand selective monofunctional uracil DNA glycosylasePRO_0000071993Add
BLAST

Proteomic databases

EPDiQ6P5C5.
MaxQBiQ6P5C5.
PaxDbiQ6P5C5.
PRIDEiQ6P5C5.

PTM databases

iPTMnetiQ6P5C5.
PhosphoSiteiQ6P5C5.

Expressioni

Gene expression databases

BgeeiQ6P5C5.
GenevisibleiQ6P5C5. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000065835.

Structurei

3D structure databases

ProteinModelPortaliQ6P5C5.
SMRiQ6P5C5. Positions 28-258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni175 – 18915DNA bindingBy similarityAdd
BLAST

Phylogenomic databases

eggNOGiENOG410IFBA. Eukaryota.
ENOG410XQUR. LUCA.
GeneTreeiENSGT00390000004897.
HOGENOMiHOG000220288.
HOVERGENiHBG084399.
InParanoidiQ6P5C5.
KOiK10800.
OMAiIMHPSPR.
OrthoDBiEOG7W41CR.
PhylomeDBiQ6P5C5.
TreeFamiTF324356.

Family and domain databases

Gene3Di3.40.470.10. 1 hit.
InterProiIPR005122. Uracil-DNA_glycosylase-like.
[Graphical view]
PfamiPF03167. UDG. 1 hit.
[Graphical view]
SUPFAMiSSF52141. SSF52141. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6P5C5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASQTFPLG PTHEPASALM EPLPCTRSLA EGFLEEELRL NAELSQLQFP
60 70 80 90 100
EPVGVIYNPV DYAWEPHRNY VTRYCQGPKE VLFLGMNPGP FGMAQTGVPF
110 120 130 140 150
GEVNVVRDWL GVGGPVLTPP QEHPKRPVLG LECPQSEVSG ARFWGFFRTL
160 170 180 190 200
CGQPQVFFRH CFVHNLCPLL FLAPSGRNLT PAELPAKQRE QLLSICDAAL
210 220 230 240 250
CRQVQLLGVR LVVGVGRLAE QRARRALAGL TPEVQVEGLL HPSPRSAQAN
260 270
KGWEAAARER LQELGLLPLL TDEGSARPT
Length:279
Mass (Da):30,654
Last modified:July 5, 2004 - v1
Checksum:iB7B70857B8351AED
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti239 – 2391L → H in BAB23517 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004735 mRNA. Translation: BAB23517.1.
BC050253 mRNA. Translation: AAH50253.1.
BC062960 mRNA. Translation: AAH62960.1.
CCDSiCCDS27898.1.
RefSeqiNP_082161.2. NM_027885.3.
XP_006521478.1. XM_006521415.2.
XP_011244032.1. XM_011245730.1.
XP_011244033.1. XM_011245731.1.
XP_011244034.1. XM_011245732.1.
XP_011244035.1. XM_011245733.1.
XP_011244036.1. XM_011245734.1.
UniGeneiMm.254820.
Mm.463445.

Genome annotation databases

EnsembliENSMUST00000064067; ENSMUSP00000065835; ENSMUSG00000036061.
GeneIDi71726.
KEGGimmu:71726.
UCSCiuc007xxh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004735 mRNA. Translation: BAB23517.1.
BC050253 mRNA. Translation: AAH50253.1.
BC062960 mRNA. Translation: AAH62960.1.
CCDSiCCDS27898.1.
RefSeqiNP_082161.2. NM_027885.3.
XP_006521478.1. XM_006521415.2.
XP_011244032.1. XM_011245730.1.
XP_011244033.1. XM_011245731.1.
XP_011244034.1. XM_011245732.1.
XP_011244035.1. XM_011245733.1.
XP_011244036.1. XM_011245734.1.
UniGeneiMm.254820.
Mm.463445.

3D structure databases

ProteinModelPortaliQ6P5C5.
SMRiQ6P5C5. Positions 28-258.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000065835.

PTM databases

iPTMnetiQ6P5C5.
PhosphoSiteiQ6P5C5.

Proteomic databases

EPDiQ6P5C5.
MaxQBiQ6P5C5.
PaxDbiQ6P5C5.
PRIDEiQ6P5C5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064067; ENSMUSP00000065835; ENSMUSG00000036061.
GeneIDi71726.
KEGGimmu:71726.
UCSCiuc007xxh.1. mouse.

Organism-specific databases

CTDi23583.
MGIiMGI:1918976. Smug1.

Phylogenomic databases

eggNOGiENOG410IFBA. Eukaryota.
ENOG410XQUR. LUCA.
GeneTreeiENSGT00390000004897.
HOGENOMiHOG000220288.
HOVERGENiHBG084399.
InParanoidiQ6P5C5.
KOiK10800.
OMAiIMHPSPR.
OrthoDBiEOG7W41CR.
PhylomeDBiQ6P5C5.
TreeFamiTF324356.

Enzyme and pathway databases

BRENDAi3.2.2.27. 3474.
ReactomeiR-MMU-110329. Cleavage of the damaged pyrimidine.
R-MMU-110357. Displacement of DNA glycosylase by APEX1.

Miscellaneous databases

PROiQ6P5C5.
SOURCEiSearch...

Gene expression databases

BgeeiQ6P5C5.
GenevisibleiQ6P5C5. MM.

Family and domain databases

Gene3Di3.40.470.10. 1 hit.
InterProiIPR005122. Uracil-DNA_glycosylase-like.
[Graphical view]
PfamiPF03167. UDG. 1 hit.
[Graphical view]
SUPFAMiSSF52141. SSF52141. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Lung.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Eye.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver, Lung, Spleen and Testis.

Entry informationi

Entry nameiSMUG1_MOUSE
AccessioniPrimary (citable) accession number: Q6P5C5
Secondary accession number(s): Q9DBV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: July 5, 2004
Last modified: June 8, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.