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Protein

Kinesin light chain 3

Gene

KLC3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Enzyme and pathway databases

ReactomeiREACT_121399. MHC class II antigen presentation.
REACT_25201. Kinesins.
REACT_355321. RHO GTPases activate KTN1.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin light chain 3
Alternative name(s):
KLC2-like
kinesin light chain 2
Gene namesi
Name:KLC3
Synonyms:KLC2, KLC2L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:20717. KLC3.

Subcellular locationi

  • Cytoplasmcytoskeleton Curated

  • Note: Elongating spermatid tail midpiece, localized in outer dense fibers (ODFs) and associates with mitochondria.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671588.

Polymorphism and mutation databases

BioMutaiKLC3.
DMDMi91207086.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 504504Kinesin light chain 3PRO_0000230785Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei466 – 4661Phosphoserine3 Publications
Modified residuei498 – 4981Phosphothreonine1 Publication
Modified residuei502 – 5021Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6P597.
PaxDbiQ6P597.
PRIDEiQ6P597.

PTM databases

PhosphoSiteiQ6P597.

Expressioni

Gene expression databases

BgeeiQ6P597.
CleanExiHS_KLC2.
HS_KLC3.
ExpressionAtlasiQ6P597. baseline and differential.
GenevestigatoriQ6P597.

Organism-specific databases

HPAiHPA052797.
HPA056508.

Interactioni

Subunit structurei

Oligomer composed of two heavy chains and two light chains. Associates with microtubulin in an ATP-dependent manner. Interacts with KIF5C and ODF1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ABI3Q9P2A43EBI-1643885,EBI-742038
BBS5Q8N3I73EBI-1643885,EBI-2892592
CCDC36Q8IYA83EBI-1643885,EBI-8638439
CCDC64BA1A5D93EBI-1643885,EBI-10171799
CCNHP519463EBI-1643885,EBI-741406
CENPPQ6IPU03EBI-1643885,EBI-10250303
CEP57L1Q8IYX8-23EBI-1643885,EBI-10181988
DTX2Q4ZH493EBI-1643885,EBI-10192429
DTX2Q86UW93EBI-1643885,EBI-740376
IMP3Q9NV313EBI-1643885,EBI-747481
KRT13A1A4E93EBI-1643885,EBI-10171552
KRT15P190123EBI-1643885,EBI-739566
MBIPQ9NS73-53EBI-1643885,EBI-10182361
NDC80O147773EBI-1643885,EBI-715849
NECAB2H3BTW23EBI-1643885,EBI-10172876
PDE4DIPQ5VU433EBI-1643885,EBI-1105124
QARSP478973EBI-1643885,EBI-347462
QARSP47897-23EBI-1643885,EBI-10209725
SNAP47Q5SQN13EBI-1643885,EBI-10244848
SPERTQ8NA613EBI-1643885,EBI-741724
STX11O755583EBI-1643885,EBI-714135
TCF4P158843EBI-1643885,EBI-533224
TSNAXQ995985EBI-1643885,EBI-742638
VPS52Q8N1B43EBI-1643885,EBI-2799833
WACQ9BTA93EBI-1643885,EBI-749118
ZNF417Q8TAU33EBI-1643885,EBI-740727
ZNF572Q7Z3I73EBI-1643885,EBI-10257016

Protein-protein interaction databases

BioGridi127078. 35 interactions.
IntActiQ6P597. 29 interactions.
MINTiMINT-3318740.
STRINGi9606.ENSP00000375810.

Structurei

3D structure databases

ProteinModelPortaliQ6P597.
SMRiQ6P597. Positions 169-442.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati207 – 24034TPR 1Add
BLAST
Repeati249 – 28234TPR 2Add
BLAST
Repeati291 – 32434TPR 3Add
BLAST
Repeati333 – 36634TPR 4Add
BLAST
Repeati375 – 40834TPR 5Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili90 – 15061Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi181 – 1844Poly-Glu
Compositional biasi190 – 1967Poly-Ala

Domaini

The heptad repeat (HR) motif is sufficient for interaction with kinesin heavy (KHL) chains and ODF1. The TPR region is involved in mitochondrial binding (By similarity).By similarity

Sequence similaritiesi

Belongs to the kinesin light chain family.Curated
Contains 5 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, TPR repeat

Phylogenomic databases

eggNOGiCOG0457.
GeneTreeiENSGT00390000006393.
HOGENOMiHOG000261663.
HOVERGENiHBG006217.
InParanoidiQ6P597.
KOiK10407.
OMAiCHPDVAT.
PhylomeDBiQ6P597.
TreeFamiTF314010.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR002151. Kinesin_light.
IPR015390. Rabaptin_Rab5-bd_dom.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF09311. Rab5-bind. 1 hit.
PF00515. TPR_1. 1 hit.
[Graphical view]
PRINTSiPR00381. KINESINLIGHT.
SMARTiSM00028. TPR. 4 hits.
[Graphical view]
PROSITEiPS50005. TPR. 4 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6P597-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVQVAAPGS AGLGPERLSP EELVRQTRQV VQGLEALRAE HHGLAGHLAE
60 70 80 90 100
ALAGQGPAAG LEMLEEKQQV VSHSLEAIEL GLGEAQVLLA LSAHVGALEA
110 120 130 140 150
EKQRLRSQAR RLAQENVWLR EELEETQRRL RASEESVAQL EEEKRHLEFL
160 170 180 190 200
GQLRQYDPPA ESQQSESPPR RDSLASLFPS EEEERKGPEA AGAAAAQQGG
210 220 230 240 250
YEIPARLRTL HNLVIQYAGQ GRYEVAVPLC RQALEDLERS SGHCHPDVAT
260 270 280 290 300
MLNILALVYR DQNKYKEATD LLHDALQIRE QTLGPEHPAV AATLNNLAVL
310 320 330 340 350
YGKRGRYREA EPLCQRALEI REKVLGADHP DVAKQLNNLA LLCQNQGKFE
360 370 380 390 400
DVERHYARAL SIYEALGGPH DPNVAKTKNN LASAYLKQNK YQQAEELYKE
410 420 430 440 450
ILHKEDLPAP LGAPNTGTAG DAEQALRRSS SLSKIRESIR RGSEKLVSRL
460 470 480 490 500
RGEAAAGAAG MKRAMSLNTL NVDAPRAPGT QFPSWHLDKA PRTLSASTQD

LSPH
Length:504
Mass (Da):55,364
Last modified:April 4, 2006 - v2
Checksum:i3767D66C79EA3D49
GO
Isoform 2 (identifier: Q6P597-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     163-163: Missing.

Note: No experimental confirmation available.

Show »
Length:503
Mass (Da):55,236
Checksum:iD8F19BD4CAE6E3AB
GO
Isoform 3 (identifier: Q6P597-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MIPQTPHHCSPGAAM

Show »
Length:518
Mass (Da):56,793
Checksum:iC49FD1AE860CB810
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti352 – 3521V → A in BAC03901 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MIPQTPHHCSPGAAM in isoform 3. 1 PublicationVSP_017831
Alternative sequencei163 – 1631Missing in isoform 2. 1 PublicationVSP_017832

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L47234 Genomic DNA. Translation: AAL48324.1.
AK092481 mRNA. Translation: BAC03901.1.
BC062998 mRNA. Translation: AAH62998.2.
BC073841 mRNA. Translation: AAH73841.1.
BC126418 mRNA. Translation: AAI26419.1.
BC133037 mRNA. Translation: AAI33038.1.
CCDSiCCDS12660.2. [Q6P597-1]
RefSeqiNP_803136.2. NM_177417.2. [Q6P597-1]
UniGeneiHs.298079.

Genome annotation databases

EnsembliENST00000391946; ENSP00000375810; ENSG00000104892. [Q6P597-1]
ENST00000470402; ENSP00000436019; ENSG00000104892. [Q6P597-3]
ENST00000585434; ENSP00000466067; ENSG00000104892. [Q6P597-2]
GeneIDi147700.
KEGGihsa:147700.
UCSCiuc002pbf.1. human. [Q6P597-1]
uc002pbg.1. human. [Q6P597-3]
uc010ejy.1. human. [Q6P597-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L47234 Genomic DNA. Translation: AAL48324.1.
AK092481 mRNA. Translation: BAC03901.1.
BC062998 mRNA. Translation: AAH62998.2.
BC073841 mRNA. Translation: AAH73841.1.
BC126418 mRNA. Translation: AAI26419.1.
BC133037 mRNA. Translation: AAI33038.1.
CCDSiCCDS12660.2. [Q6P597-1]
RefSeqiNP_803136.2. NM_177417.2. [Q6P597-1]
UniGeneiHs.298079.

3D structure databases

ProteinModelPortaliQ6P597.
SMRiQ6P597. Positions 169-442.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127078. 35 interactions.
IntActiQ6P597. 29 interactions.
MINTiMINT-3318740.
STRINGi9606.ENSP00000375810.

PTM databases

PhosphoSiteiQ6P597.

Polymorphism and mutation databases

BioMutaiKLC3.
DMDMi91207086.

Proteomic databases

MaxQBiQ6P597.
PaxDbiQ6P597.
PRIDEiQ6P597.

Protocols and materials databases

DNASUi147700.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000391946; ENSP00000375810; ENSG00000104892. [Q6P597-1]
ENST00000470402; ENSP00000436019; ENSG00000104892. [Q6P597-3]
ENST00000585434; ENSP00000466067; ENSG00000104892. [Q6P597-2]
GeneIDi147700.
KEGGihsa:147700.
UCSCiuc002pbf.1. human. [Q6P597-1]
uc002pbg.1. human. [Q6P597-3]
uc010ejy.1. human. [Q6P597-2]

Organism-specific databases

CTDi147700.
GeneCardsiGC19P045851.
HGNCiHGNC:20717. KLC3.
HPAiHPA052797.
HPA056508.
MIMi601334. gene.
neXtProtiNX_Q6P597.
PharmGKBiPA142671588.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0457.
GeneTreeiENSGT00390000006393.
HOGENOMiHOG000261663.
HOVERGENiHBG006217.
InParanoidiQ6P597.
KOiK10407.
OMAiCHPDVAT.
PhylomeDBiQ6P597.
TreeFamiTF314010.

Enzyme and pathway databases

ReactomeiREACT_121399. MHC class II antigen presentation.
REACT_25201. Kinesins.
REACT_355321. RHO GTPases activate KTN1.

Miscellaneous databases

GeneWikiiKLC3.
GenomeRNAii147700.
NextBioi85698.
PROiQ6P597.
SOURCEiSearch...

Gene expression databases

BgeeiQ6P597.
CleanExiHS_KLC2.
HS_KLC3.
ExpressionAtlasiQ6P597. baseline and differential.
GenevestigatoriQ6P597.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR002151. Kinesin_light.
IPR015390. Rabaptin_Rab5-bd_dom.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF09311. Rab5-bind. 1 hit.
PF00515. TPR_1. 1 hit.
[Graphical view]
PRINTSiPR00381. KINESINLIGHT.
SMARTiSM00028. TPR. 4 hits.
[Graphical view]
PROSITEiPS50005. TPR. 4 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the ERCC2 gene regions in human, mouse, and hamster reveals three linked genes."
    Lamerdin J.E., Stilwagen S.A., Ramirez M.H., Stubbs L., Carrano A.V.
    Genomics 34:399-409(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Fibroblast.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Placenta.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain and Colon.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-466 AND SER-502, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-466, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  7. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-466; THR-498 AND SER-502, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKLC3_HUMAN
AccessioniPrimary (citable) accession number: Q6P597
Secondary accession number(s): A0AVM3
, A2RUT6, Q6GMU2, Q8NAL1, Q8WWJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: April 4, 2006
Last modified: May 27, 2015
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.