Q6P597 (KLC3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kinesin light chain 3

Alternative name(s):
KLC2-like
kinesin light chain 2

Gene names

Name:	KLC3
Synonyms:	KLC2, KLC2L

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport.
Subunit structure	Oligomer composed of two heavy chains and two light chains. Associates with microtubulin in an ATP-dependent manner. Interacts with KIF5C and ODF1 By similarity.
Subcellular location	Cytoplasm › cytoskeleton Probable. Note: Elongating spermatid tail midpiece, localized in outer dense fibers (ODFs) and associates with mitochondria By similarity.
Domain	The heptad repeat (HR) motif is sufficient for interaction with kinesin heavy (KHL) chains and ODF1. The TPR region is involved in mitochondrial binding By similarity.
Sequence similarities	Belongs to the kinesin light chain family. Contains 5 TPR repeats.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Repeat TPR repeat
Molecular function	Motor protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	ciliary rootlet Inferred from electronic annotation. Source: Compara cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW kinesin complex Inferred from electronic annotation. Source: InterPro microtubule Inferred from electronic annotation. Source: UniProtKB-KW neuron projection Inferred from electronic annotation. Source: Compara
Molecular_function	cardiolipin synthase activity Inferred from electronic annotation. Source: Compara microtubule motor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6P597-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6P597-2)

The sequence of this isoform differs from the canonical sequence as follows:
     163-163: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q6P597-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MIPQTPHHCSPGAAM

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	Kinesin light chain 3		PRO_0000230785
Regions
	Repeat	207 – 240	34	TPR 1
	Repeat	249 – 282	34	TPR 2
	Repeat	291 – 324	34	TPR 3
	Repeat	333 – 366	34	TPR 4
	Repeat	375 – 408	34	TPR 5
	Coiled coil	90 – 150	61	Potential
	Compositional bias	181 – 184	4	Poly-Glu
	Compositional bias	190 – 196	7	Poly-Ala
Amino acid modifications
	Modified residue	173	1	Phosphoserine By similarity
	Modified residue	466	1	Phosphoserine Ref.4 Ref.5 Ref.6
	Modified residue	498	1	Phosphothreonine Ref.6
	Modified residue	502	1	Phosphoserine Ref.4 Ref.6
Natural variations
	Alternative sequence	1	1	M → MIPQTPHHCSPGAAM in isoform 3.		VSP_017831
	Alternative sequence	163	1	Missing in isoform 2.		VSP_017832
Experimental info
	Sequence conflict	352	1	V → A in BAC03901. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 4, 2006. Version 2. Checksum: 3767D66C79EA3D49	FASTA	504	55,364
	10 20 30 40 50 60 MSVQVAAPGS AGLGPERLSP EELVRQTRQV VQGLEALRAE HHGLAGHLAE ALAGQGPAAG 70 80 90 100 110 120 LEMLEEKQQV VSHSLEAIEL GLGEAQVLLA LSAHVGALEA EKQRLRSQAR RLAQENVWLR 130 140 150 160 170 180 EELEETQRRL RASEESVAQL EEEKRHLEFL GQLRQYDPPA ESQQSESPPR RDSLASLFPS 190 200 210 220 230 240 EEEERKGPEA AGAAAAQQGG YEIPARLRTL HNLVIQYAGQ GRYEVAVPLC RQALEDLERS 250 260 270 280 290 300 SGHCHPDVAT MLNILALVYR DQNKYKEATD LLHDALQIRE QTLGPEHPAV AATLNNLAVL 310 320 330 340 350 360 YGKRGRYREA EPLCQRALEI REKVLGADHP DVAKQLNNLA LLCQNQGKFE DVERHYARAL 370 380 390 400 410 420 SIYEALGGPH DPNVAKTKNN LASAYLKQNK YQQAEELYKE ILHKEDLPAP LGAPNTGTAG 430 440 450 460 470 480 DAEQALRRSS SLSKIRESIR RGSEKLVSRL RGEAAAGAAG MKRAMSLNTL NVDAPRAPGT 490 500 QFPSWHLDKA PRTLSASTQD LSPH
	Isoform 2 [UniParc]. Checksum: D8F19BD4CAE6E3AB	FASTA	503	55,236
	10 20 30 40 50 60 MSVQVAAPGS AGLGPERLSP EELVRQTRQV VQGLEALRAE HHGLAGHLAE ALAGQGPAAG 70 80 90 100 110 120 LEMLEEKQQV VSHSLEAIEL GLGEAQVLLA LSAHVGALEA EKQRLRSQAR RLAQENVWLR 130 140 150 160 170 180 EELEETQRRL RASEESVAQL EEEKRHLEFL GQLRQYDPPA ESQSESPPRR DSLASLFPSE 190 200 210 220 230 240 EEERKGPEAA GAAAAQQGGY EIPARLRTLH NLVIQYAGQG RYEVAVPLCR QALEDLERSS 250 260 270 280 290 300 GHCHPDVATM LNILALVYRD QNKYKEATDL LHDALQIREQ TLGPEHPAVA ATLNNLAVLY 310 320 330 340 350 360 GKRGRYREAE PLCQRALEIR EKVLGADHPD VAKQLNNLAL LCQNQGKFED VERHYARALS 370 380 390 400 410 420 IYEALGGPHD PNVAKTKNNL ASAYLKQNKY QQAEELYKEI LHKEDLPAPL GAPNTGTAGD 430 440 450 460 470 480 AEQALRRSSS LSKIRESIRR GSEKLVSRLR GEAAAGAAGM KRAMSLNTLN VDAPRAPGTQ 490 500 FPSWHLDKAP RTLSASTQDL SPH
	Isoform 3 [UniParc]. Checksum: C49FD1AE860CB810	FASTA	518	56,793
	10 20 30 40 50 60 MIPQTPHHCS PGAAMSVQVA APGSAGLGPE RLSPEELVRQ TRQVVQGLEA LRAEHHGLAG 70 80 90 100 110 120 HLAEALAGQG PAAGLEMLEE KQQVVSHSLE AIELGLGEAQ VLLALSAHVG ALEAEKQRLR 130 140 150 160 170 180 SQARRLAQEN VWLREELEET QRRLRASEES VAQLEEEKRH LEFLGQLRQY DPPAESQQSE 190 200 210 220 230 240 SPPRRDSLAS LFPSEEEERK GPEAAGAAAA QQGGYEIPAR LRTLHNLVIQ YAGQGRYEVA 250 260 270 280 290 300 VPLCRQALED LERSSGHCHP DVATMLNILA LVYRDQNKYK EATDLLHDAL QIREQTLGPE 310 320 330 340 350 360 HPAVAATLNN LAVLYGKRGR YREAEPLCQR ALEIREKVLG ADHPDVAKQL NNLALLCQNQ 370 380 390 400 410 420 GKFEDVERHY ARALSIYEAL GGPHDPNVAK TKNNLASAYL KQNKYQQAEE LYKEILHKED 430 440 450 460 470 480 LPAPLGAPNT GTAGDAEQAL RRSSSLSKIR ESIRRGSEKL VSRLRGEAAA GAAGMKRAMS 490 500 510 LNTLNVDAPR APGTQFPSWH LDKAPRTLSA STQDLSPH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the ERCC2 gene regions in human, mouse, and hamster reveals three linked genes." Lamerdin J.E., Stilwagen S.A., Ramirez M.H., Stubbs L., Carrano A.V. Genomics 34:399-409(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Fibroblast.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Placenta.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Colon.
[4]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-466 AND SER-502, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[5]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-466, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[6]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-466; THR-498 AND SER-502, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L47234 Genomic DNA. Translation: AAL48324.1. AK092481 mRNA. Translation: BAC03901.1. BC062998 mRNA. Translation: AAH62998.2. BC073841 mRNA. Translation: AAH73841.1. BC126418 mRNA. Translation: AAI26419.1. BC133037 mRNA. Translation: AAI33038.1.
IPI	IPI00465074. IPI00478456. IPI00736469.
RefSeq	NP_803136.2. NM_177417.2.
UniGene	Hs.298079.
3D structure databases
ProteinModelPortal	Q6P597.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6P597. 1 interaction.
MINT	MINT-3318740.
STRING	9606.ENSP00000375810.
PTM databases
PhosphoSite	Q6P597.
Polymorphism databases
DMDM	91207086.
Proteomic databases
PaxDb	Q6P597.
PRIDE	Q6P597.
Protocols and materials databases
DNASU	147700.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000391946; ENSP00000375810; ENSG00000104892. ENST00000470402; ENSP00000436019; ENSG00000104892. ENST00000585434; ENSP00000466067; ENSG00000104892.
GeneID	147700.
KEGG	hsa:147700.
UCSC	uc002pbf.1. human. uc002pbg.1. human. uc010ejy.1. human.
Organism-specific databases
CTD	147700.
GeneCards	GC19P045851.
HGNC	HGNC:20717. KLC3.
HPA	HPA052797.
MIM	601334. gene.
neXtProt	NX_Q6P597.
PharmGKB	PA142671588.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0457.
HOGENOM	HOG000261663.
HOVERGEN	HBG006217.
InParanoid	Q6P597.
KO	K10407.
OMA	YGKRGRY.
OrthoDB	EOG4D26PZ.
Enzyme and pathway databases
Reactome	REACT_604. Hemostasis. REACT_6900. Immune System.
Gene expression databases
Bgee	Q6P597.
CleanEx	HS_KLC2. HS_KLC3.
Genevestigator	Q6P597.
GermOnline	ENSG00000104892. Homo sapiens.
Family and domain databases
Gene3D	1.25.40.10. 1 hit.
InterPro	IPR002151. Kinesin_light. IPR015390. Rabaptin_Rab5-bd_dom. IPR001440. TPR-1. IPR013026. TPR-contain_dom. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
Pfam	PF09311. Rab5-bind. 1 hit. PF00515. TPR_1. 1 hit. [Graphical view]
PRINTS	PR00381. KINESINLIGHT.
SMART	SM00028. TPR. 4 hits. [Graphical view]
PROSITE	PS50005. TPR. 4 hits. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	147700.
NextBio	85698.
SOURCE	Search...

Entry information

Entry name

KLC3_HUMAN

Accession

Primary (citable) accession number: Q6P597
Secondary accession number(s): A0AVM3 , A2RUT6, Q6GMU2, Q8NAL1, Q8WWJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	April 4, 2006
Last modified:	May 1, 2013
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.