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Q6P542 (ABCF1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-binding cassette sub-family F member 1
Gene names
Name:Abcf1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length837 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for efficient Cap- and IRES-mediated mRNA translation initiation. Not involved in the ribosome biogenesis By similarity.

Subunit structure

Interacts (via N-terminus) with EIF2S1; the interaction is independent of its phosphorylated status. Associates (via both ABC transporter domains) with the ribosomes By similarity.

Subcellular location

Cytoplasm By similarity. Nucleusnucleoplasm By similarity. Nucleus envelope By similarity.

Post-translational modification

Phosphorylated at phosphoserine and phosphothreonine. Phosphorylation on Ser-107 and Ser-138 by CK2; inhibits association of EIF2 with ribosomes By similarity.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.

Contains 2 ABC transporter domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 837837ATP-binding cassette sub-family F member 1
PRO_0000093319

Regions

Domain296 – 540245ABC transporter 1
Domain617 – 832216ABC transporter 2
Nucleotide binding328 – 3358ATP 1 By similarity
Nucleotide binding650 – 6578ATP 2 By similarity
Compositional bias139 – 23395Glu-rich

Amino acid modifications

Modified residue221Phosphoserine By similarity
Modified residue901Phosphoserine Ref.3
Modified residue1031Phosphoserine Ref.2 Ref.3
Modified residue1071Phosphoserine; by CK2 Ref.2 Ref.3 Ref.4 Ref.6
Modified residue1381Phosphoserine; by CK2 Ref.2 Ref.3 Ref.5
Modified residue1941Phosphoserine Ref.3 Ref.4
Modified residue5871Phosphoserine By similarity
Cross-link565Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-link574Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6P542 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 9CCD1753D5357944

FASTA83794,945
        10         20         30         40         50         60 
MPKGPKQQPP EPEWIGDGEG TSPADKVVKK GKKDKKTKKT FFEELAVEDK QAGEEEKLQK 

        70         80         90        100        110        120 
EKEQQQQQQQ QKKKRDTRKG RRKKDVDDDS DERVLMERLK QLSVPASDEE DEVPAPIPRG 

       130        140        150        160        170        180 
RKKAKGGNVF EALIQDDSEE EEEEEENRVL KPAKPEKNRI NKAVAEEPPG LRSKKGKEEK 

       190        200        210        220        230        240 
SKGKAKSKPA AADSEGEEEE EDTAKEKEPP QQGKDRDKKE AEQGSGEEKE EKEGDLKAND 

       250        260        270        280        290        300 
PYANLSKKEK KKLKKQMDYE RQVESLKAAN AAENDFSVSQ AEVSSRQAML ENASDIKLEK 

       310        320        330        340        350        360 
FSISAHGKEL FVNADLYIVA GRRYGLVGPN GKGKTTLLKH IANRALSIPP NIDVLLCEQE 

       370        380        390        400        410        420 
VVADETPAVQ AVLRADTKRL RLLEEERRLQ GQLEQGDDTA AEKLEKVYEE LRATGAAAAE 

       430        440        450        460        470        480 
AKARRILAGL GFDPEMQNRP TQKFSGGWRM RVSLARALFM EPTLLMLDEP TNHLDLNAVI 

       490        500        510        520        530        540 
WLNNYLQGWR KTLLIVSHDQ GFLDDVCTDI IHLDTQRLHY YRGNYMTFKK MYQQKQKELL 

       550        560        570        580        590        600 
KQYEKQEKKL KELKAGGKST KQAEKQTKEV LTRKQQKCRR KNQDEESQEP PELLKRPKEY 

       610        620        630        640        650        660 
TVRFTFPDPP PLSPPVLGLH GVTFGYEGQK PLFKNLDFGI DMDSRICIVG PNGVGKSTLL 

       670        680        690        700        710        720 
LLLTGKLTPT NGEMRKNHRL KIGFFNQQYA EQLHMEETPT EYLQRSFNLP YQDARKCLGR 

       730        740        750        760        770        780 
FGLESHAHTI QICKLSGGQK ARVVFAELAC REPDVLILDE PTNNLDIESI DALGEAINDY 

       790        800        810        820        830 
KGAVIVVSHD ARLITETNCQ LWVVEEQGVS QIDGDFDDYK REVLEALGEV MVNRPRD 

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: ICR.
Tissue: Limb and Trophoblast stem cell.
[2]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-103; SER-107 AND SER-138, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.
[3]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-90; SER-103; SER-107; SER-138 AND SER-194, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[4]"Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-107 AND SER-194, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[5]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-138, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-107, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC063094 mRNA. Translation: AAH63094.1.
BC068282 mRNA. Translation: AAH68282.1.
CCDSCCDS28713.1.
RefSeqNP_038882.1. NM_013854.1.
UniGeneMm.329022.

3D structure databases

ProteinModelPortalQ6P542.
SMRQ6P542. Positions 293-828.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid230310. 1 interaction.
MINTMINT-1859549.

PTM databases

PhosphoSiteQ6P542.

Proteomic databases

MaxQBQ6P542.
PaxDbQ6P542.
PRIDEQ6P542.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000043757; ENSMUSP00000036881; ENSMUSG00000038762.
GeneID224742.
KEGGmmu:224742.
UCSCuc008cjk.1. mouse.

Organism-specific databases

CTD23.
MGIMGI:1351658. Abcf1.

Phylogenomic databases

eggNOGCOG0488.
GeneTreeENSGT00630000089843.
HOGENOMHOG000271637.
HOVERGENHBG050440.
InParanoidQ6P542.
KOK06184.
OMAVPYEEYL.
OrthoDBEOG7WDN23.
PhylomeDBQ6P542.
TreeFamTF105207.

Gene expression databases

ArrayExpressQ6P542.
BgeeQ6P542.
GenevestigatorQ6P542.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMSSF52540. SSF52540. 3 hits.
PROSITEPS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio377328.
PROQ6P542.
SOURCESearch...

Entry information

Entry nameABCF1_MOUSE
AccessionPrimary (citable) accession number: Q6P542
Secondary accession number(s): Q6NV71
Entry history
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot