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Protein

Threonylcarbamoyladenosine tRNA methylthiotransferase

Gene

cdkal1

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t6A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms2t6A) at position 37 in tRNAs that read codons beginning with adenine.By similarity

Catalytic activityi

N(6)-L-threonylcarbamoyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N(6)-L-threonylcarbamoyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.By similarity

Cofactori

[4Fe-4S] clusterPROSITE-ProRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi69 – 691Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi105 – 1051Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi134 – 1341Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi210 – 2101Iron-sulfur (4Fe-4S-S-AdoMet)PROSITE-ProRule annotation
Metal bindingi214 – 2141Iron-sulfur (4Fe-4S-S-AdoMet)PROSITE-ProRule annotation
Metal bindingi217 – 2171Iron-sulfur (4Fe-4S-S-AdoMet)PROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Threonylcarbamoyladenosine tRNA methylthiotransferase (EC:2.8.4.5)
Alternative name(s):
CDK5 regulatory subunit-associated protein 1-like 1
tRNA-t(6)A37 methylthiotransferase
Gene namesi
Name:cdkal1
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
Proteomesi
  • UP000008143 Componenti: Unassembled WGS sequence

Organism-specific databases

XenbaseiXB-GENE-5895778. cdkal1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei530 – 55021HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 553553Threonylcarbamoyladenosine tRNA methylthiotransferasePRO_0000298674Add
BLAST

Proteomic databases

PaxDbiQ6P4Y0.

Interactioni

Protein-protein interaction databases

STRINGi8364.ENSXETP00000000030.

Structurei

3D structure databases

ProteinModelPortaliQ6P4Y0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini60 – 168109MTTase N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini427 – 48963TRAMPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 MTTase N-terminal domain.PROSITE-ProRule annotation
Contains 1 TRAM domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4355. Eukaryota.
COG0621. LUCA.
HOGENOMiHOG000224768.
HOVERGENiHBG097712.
InParanoidiQ6P4Y0.
KOiK15865.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006466. MiaB-like_B.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01578. MiaB-like-B. 1 hit.
TIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6P4Y0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAACESLLD DIEDIVSATD PKPHDRQNAR QNIVPRARKR NKNKIQEEEP
60 70 80 90 100
PADSTIPGTQ KIWIRTWGCS HNNSDGEYMA GQLAAYGYSI TEQPEQADLW
110 120 130 140 150
LLNSCTVKSP AEDHFRNSIK KAQEANKKVV LSGCVPQAQP RQEYMKGLSI
160 170 180 190 200
IGVQQIDRVV EVVEETIKGH SVRLLGQKKD NGKRLGGARL DLPKIRKNPL
210 220 230 240 250
IEIISINTGC LNACTYCKTK HARGELASYP VEELVDRAAQ SFQEGVCEIW
260 270 280 290 300
LTSEDTGAYG RDIGTDLPTL LWKLVEVIPE GAMLRLGMTN PPYILEHLEE
310 320 330 340 350
MAKILNHPRV YAFLHIPVQS ASDSVLMDMK REYCIADFKR VVDFLKERVP
360 370 380 390 400
GITIATDIIC GFPGETDEDF KETLKLVEEY KFPSLFINQF YPRPGTPAAK
410 420 430 440 450
MEQVPAHVKK QRTKELSQLF HSYSPYDHKI GEEQHVLVTE ESFDSQYYVS
460 470 480 490 500
HNRFYEQVLV PKDPAFVGKM VEVKIFEAGK HFMKGQPVQD SYIYTPSITK
510 520 530 540 550
PLAKGEVSGL TEESKPPNNP ESLLQTSREG LQTFFFVTAL LAAVIAFVGI

KLL
Length:553
Mass (Da):62,250
Last modified:July 5, 2004 - v1
Checksum:iA60BF0F555697835
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC063205 mRNA. Translation: AAH63205.1.
RefSeqiNP_989194.1. NM_203863.1.
XP_012819892.1. XM_012964438.1.
UniGeneiStr.1797.
Str.53242.

Genome annotation databases

GeneIDi394802.
KEGGixtr:394802.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC063205 mRNA. Translation: AAH63205.1.
RefSeqiNP_989194.1. NM_203863.1.
XP_012819892.1. XM_012964438.1.
UniGeneiStr.1797.
Str.53242.

3D structure databases

ProteinModelPortaliQ6P4Y0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi8364.ENSXETP00000000030.

Proteomic databases

PaxDbiQ6P4Y0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi394802.
KEGGixtr:394802.

Organism-specific databases

CTDi54901.
XenbaseiXB-GENE-5895778. cdkal1.

Phylogenomic databases

eggNOGiKOG4355. Eukaryota.
COG0621. LUCA.
HOGENOMiHOG000224768.
HOVERGENiHBG097712.
InParanoidiQ6P4Y0.
KOiK15865.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006466. MiaB-like_B.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01578. MiaB-like-B. 1 hit.
TIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.

Entry informationi

Entry nameiCDKAL_XENTR
AccessioniPrimary (citable) accession number: Q6P4Y0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: July 5, 2004
Last modified: July 6, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.