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Q6P4T2 (U520_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
U5 small nuclear ribonucleoprotein 200 kDa helicase

EC=3.6.4.13
Alternative name(s):
BRR2 homolog
U5 snRNP-specific 200 kDa protein
Short name=U5-200KD
Gene names
Name:Snrnp200
Synonyms:Kiaa0788
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length2136 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RNA helicase that plays an essential role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes. Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome. Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Component of a core complex containing at least PRPF8, SNRNP200, EFTUD2 and SNRNP40. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, NHP2L1, EFTUD2, SART1 and USP39. Identified in the spliceosome C complex By similarity.

Subcellular location

Nucleus By similarity.

Domain

Contains two helicase domains By similarity. The N-terminal helicase domain has catalytic activity by itself, contrary to C-terminal helicase domain that may have a regulatory role and enhance the activity of the first helicase domain By similarity.

Sequence similarities

Belongs to the helicase family. SKI2 subfamily.

Contains 2 helicase ATP-binding domains.

Contains 2 helicase C-terminal domains.

Contains 2 SEC63 domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 21362136U5 small nuclear ribonucleoprotein 200 kDa helicase
PRO_0000422049

Regions

Domain490 – 673184Helicase ATP-binding 1
Domain684 – 921238Helicase C-terminal 1
Domain982 – 1286305SEC63 1
Domain1337 – 1512176Helicase ATP-binding 2
Domain1545 – 1753209Helicase C-terminal 2
Domain1812 – 2124313SEC63 2
Nucleotide binding503 – 5108ATP By similarity
Nucleotide binding1350 – 13578ATP By similarity
Coiled coil54 – 8431 Potential
Motif615 – 6184DEAH box
Motif1454 – 14574DEAH box

Amino acid modifications

Modified residue261Phosphoserine By similarity
Modified residue2251Phosphoserine Ref.4 Ref.7
Modified residue7091Phosphotyrosine By similarity
Modified residue9711N6-acetyllysine; alternate By similarity
Modified residue17651Phosphothreonine Ref.5
Modified residue21311Phosphothreonine By similarity
Modified residue21331Phosphoserine By similarity
Modified residue21351Phosphoserine By similarity
Cross-link944Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Cross-link971Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate By similarity
Cross-link1071Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Cross-link1199Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Cross-link2091Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6P4T2 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: BBDD6058CA635519

FASTA2,136244,547
        10         20         30         40         50         60 
MADVTARSLQ YEYKANSNLV LQADRSLIDR TRRDEPTGEV LSLVGKLEGT RMGDKAQRTK 

        70         80         90        100        110        120 
PQMQEERRAK RRKRDEDRHD MNKMKGYTLL SEGIDEMVGI IYKPKTKETR ETYEVLLSFI 

       130        140        150        160        170        180 
QAALGDQPRD ILCGAADEVL AVLKNEKLRD KERRREIDLL LGQTDDTRYH VLVNLGKKIT 

       190        200        210        220        230        240 
DYGGDKEIQN MDDNIDETYG VNVQFESDEE EGDEDVYGEV REEASDDDME GDEAVVRCTL 

       250        260        270        280        290        300 
SANLVASGEL MSSKKKDLHP RDIDAFWLQR QLSRFYDDAI VSQKKADEVL EILKTASDDR 

       310        320        330        340        350        360 
ECENQLVLLL GFNTFDFIKV LRQHRMMILY CTLLASAQSE PEKERIVGKM EADPELSKFL 

       370        380        390        400        410        420 
YQLHETEKED LIREERSRRE RVRQSRMDTD LETMDLDQGG EALAPRQVLD LEDLVFTQGS 

       430        440        450        460        470        480 
HFMANKRCQL PDGSFRRQRK GYEEVHVPAL KPKPFGSEEQ LLPVEKLPKY AQAGFEGFKT 

       490        500        510        520        530        540 
LNRIQSKLYR AALETDENLL LCAPTGAGKT NVALMCMLRE IGKHINMDGT INVDDFKIIY 

       550        560        570        580        590        600 
IAPMRSLVQE MVGSFGKRLA TYGITVAELT GDHQLCKEEI SATQIIVCTP EKWDIITRKG 

       610        620        630        640        650        660 
GERTYTQLVR LIVLDEIHLL HDDRGPVLEA LVARAIRNIE MTQEDVRLIG LSATLPNYED 

       670        680        690        700        710        720 
VATFLRVDPA KGLFYFDNSF RPVPLEQTYV GITEKKAIKR FQIMNEIVYE KIMEHAGKNQ 

       730        740        750        760        770        780 
VLVFVHSRKE TGKTARAIRD MCLEKDTLGL FLREGSASTE VLRTEAEQCK NLELKDLLPY 

       790        800        810        820        830        840 
GFAIHHAGMT RVDRTLVEDL FADKHIQVLV STATLAWGVN LPAHTVIIKG TQVYSPEKGR 

       850        860        870        880        890        900 
WTELGALDIL QMLGRAGRPQ YDTKGEGILI TSHGELQYYL SLLNQQLPIE SQMVSKLPDM 

       910        920        930        940        950        960 
LNAEIVLGNV QNAKDAVNWL GYAYLYIRML RSPTLYGISH DDLKGDPLLD QRRLDLVHTA 

       970        980        990       1000       1010       1020 
ALMLDKNNLV KYDKKTGNFQ VTELGRIASH YYITNDTVQT YNQLLKPTLS EIELFRVFSL 

      1030       1040       1050       1060       1070       1080 
SSEFKNITVR EEEKLELQKL LERVPIPVKE SIEEPSAKIN VLLQAFISQL KLEGFALMAD 

      1090       1100       1110       1120       1130       1140 
MVYVTQSAGR LMRAIFEIVL NRGWAQLTDK TLNLCKMIDK RMWQSMCPLR QFRKLPEEVV 

      1150       1160       1170       1180       1190       1200 
KKIEKKNFPF ERLYDLNHNE IGELIRMPKM GKTIHKYVHL FPKLELSVHL QPITRSTLKV 

      1210       1220       1230       1240       1250       1260 
ELTITPDFQW DEKVHGSSEA FWILVEDVDS EVILHHEYFL LKAKYAQDEH LITFFVPVFE 

      1270       1280       1290       1300       1310       1320 
PLPPQYFIRV VSDRWLSCET QLPVSFRHLI LPEKYPPPTE LLDLQPLPVS ALRNSAFESL 

      1330       1340       1350       1360       1370       1380 
YQDKFPFFNP IQTQVFNTVY NSDDNVFVGA PTGSGKTICA EFAILRMLLQ NSEGRCVYIT 

      1390       1400       1410       1420       1430       1440 
PMEALAEQVY MDWYEKFQDR LNKKVVLLTG ETSTDLKLLG KGNIIISTPE KWDILSRRWK 

      1450       1460       1470       1480       1490       1500 
QRKNVQNINL FVVDEVHLIG GENGPVLEVI CSRMRYISSQ IERPIRIVAL SSSLSNAKDV 

      1510       1520       1530       1540       1550       1560 
AHWLGCSATS TFNFHPNVRP VPLELHIQGF NISHTQTRLL SMAKPVYHAI TKHSPKKPVI 

      1570       1580       1590       1600       1610       1620 
VFVPSRKQTR LTAIDILTTC AADIQRQRFL HCTEKDLIPY LEKLSDSTLK ETLLNGVGYL 

      1630       1640       1650       1660       1670       1680 
HEGLSPMERR LVEQLFSSGA IQVVVASRSL CWGMNVAAHL VIIMDTQYYN GKIHAYVDYP 

      1690       1700       1710       1720       1730       1740 
IYDVLQMVGH ANRPLQDDEG RCVIMCQGSK KDFFKKFLYE PLPVESHLDH CMHDHFNAEI 

      1750       1760       1770       1780       1790       1800 
VTKTIENKQD AVDYLTWTFL YRRMTQNPNY YNLQGISHRH LSDHLSELVE QTLSDLEQSK 

      1810       1820       1830       1840       1850       1860 
CISIEDEMDV APLNLGMIAA YYYINYTTIE LFSMSLNAKT KVRGLIEIIS NAAEYENIPI 

      1870       1880       1890       1900       1910       1920 
RHHEDNLLRQ LAQKVPHKLN NPKFNDPHVK TNLLLQAHLS RMQLSAELQS DTEEILSKAI 

      1930       1940       1950       1960       1970       1980 
RLIQACVDVL SSNGWLSPAL AAMELAQMVT QAMWSKDSYL KQLPHFTSEH IKRCTDKGVE 

      1990       2000       2010       2020       2030       2040 
SVFDIMEMED EERNALLQLT DSQIADVARF CNRYPNIELS YEVVDKDSIR SGGPVVVLVQ 

      2050       2060       2070       2080       2090       2100 
LEREEEVTGP VIAPLFPQKR EEGWWVVIGD AKSNSLISIK RLTLQQKAKV KLDFVAPATG 

      2110       2120       2130 
GHNYTLYFMS DAYMGCDQEY KFSVDVKEAE TDSDSD 

« Hide

References

[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[3]"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 228-2136.
Tissue: Embryonic tail.
[4]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[5]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1765, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
[6]"Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[7]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL845368 Genomic DNA. No translation available.
BC063261 mRNA. Translation: AAH63261.1.
AK173025 mRNA. Translation: BAD32303.1.
CCDSCCDS16695.1.
RefSeqNP_796188.2. NM_177214.4.
UniGeneMm.215860.

3D structure databases

ProteinModelPortalQ6P4T2.
SMRQ6P4T2. Positions 1840-2129.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ6P4T2. 2 interactions.
MINTMINT-8174184.

Proteomic databases

MaxQBQ6P4T2.
PRIDEQ6P4T2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000103220; ENSMUSP00000099509; ENSMUSG00000003660.
GeneID320632.
KEGGmmu:320632.
UCSCuc008mez.1. mouse.

Organism-specific databases

CTD23020.
MGIMGI:2444401. Snrnp200.
RougeSearch...

Phylogenomic databases

GeneTreeENSGT00640000091272.
HOGENOMHOG000157749.
HOVERGENHBG051896.
InParanoidQ6P4T2.
KOK12854.
OMAKKMENWW.
OrthoDBEOG7PGDPQ.
PhylomeDBQ6P4T2.
TreeFamTF300056.

Gene expression databases

GenevestigatorQ6P4T2.

Family and domain databases

Gene3D2.60.40.150. 1 hit.
3.40.50.300. 4 hits.
InterProIPR003593. AAA+_ATPase.
IPR000008. C2_dom.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR014756. Ig_E-set.
IPR027417. P-loop_NTPase.
IPR004179. Sec63-dom.
[Graphical view]
PfamPF00270. DEAD. 2 hits.
PF00271. Helicase_C. 2 hits.
PF02889. Sec63. 2 hits.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
SM00487. DEXDc. 2 hits.
SM00490. HELICc. 2 hits.
SM00611. SEC63. 2 hits.
SM00973. Sec63. 2 hits.
[Graphical view]
SUPFAMSSF52540. SSF52540. 4 hits.
SSF81296. SSF81296. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 2 hits.
PS51194. HELICASE_CTER. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSNRNP200. mouse.
NextBio397101.
PROQ6P4T2.
SOURCESearch...

Entry information

Entry nameU520_MOUSE
AccessionPrimary (citable) accession number: Q6P4T2
Secondary accession number(s): Q69ZZ3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot