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Q6P4T2

- U520_MOUSE

UniProt

Q6P4T2 - U520_MOUSE

Protein

U5 small nuclear ribonucleoprotein 200 kDa helicase

Gene

Snrnp200

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    RNA helicase that plays an essential role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes. Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome. Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome By similarity.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi503 – 5108ATPPROSITE-ProRule annotation
    Nucleotide bindingi1350 – 13578ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent RNA helicase activity Source: UniProtKB
    3. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. mRNA processing Source: UniProtKB-KW
    3. RNA splicing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Helicase, Hydrolase, Ribonucleoprotein

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    U5 small nuclear ribonucleoprotein 200 kDa helicase (EC:3.6.4.13)
    Alternative name(s):
    BRR2 homolog
    U5 snRNP-specific 200 kDa protein
    Short name:
    U5-200KD
    Gene namesi
    Name:Snrnp200
    Synonyms:Kiaa0788
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:2444401. Snrnp200.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. catalytic step 2 spliceosome Source: Ensembl
    2. U5 snRNP Source: Ensembl

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 21362136U5 small nuclear ribonucleoprotein 200 kDa helicasePRO_0000422049Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei26 – 261PhosphoserineBy similarity
    Modified residuei225 – 2251Phosphoserine2 Publications
    Modified residuei709 – 7091PhosphotyrosineBy similarity
    Cross-linki944 – 944Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Modified residuei971 – 9711N6-acetyllysine; alternateBy similarity
    Cross-linki971 – 971Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
    Cross-linki1071 – 1071Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Cross-linki1199 – 1199Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Modified residuei1765 – 17651Phosphothreonine1 Publication
    Cross-linki2091 – 2091Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Modified residuei2131 – 21311PhosphothreonineBy similarity
    Modified residuei2133 – 21331PhosphoserineBy similarity
    Modified residuei2135 – 21351PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ6P4T2.
    PRIDEiQ6P4T2.

    Expressioni

    Gene expression databases

    GenevestigatoriQ6P4T2.

    Interactioni

    Subunit structurei

    Component of a core complex containing at least PRPF8, SNRNP200, EFTUD2 and SNRNP40. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, NHP2L1, EFTUD2, SART1 and USP39. Identified in the spliceosome C complex By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ6P4T2. 2 interactions.
    MINTiMINT-8174184.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6P4T2.
    SMRiQ6P4T2. Positions 1840-2129.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini490 – 673184Helicase ATP-binding 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini684 – 921238Helicase C-terminal 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini982 – 1286305SEC63 1Add
    BLAST
    Domaini1337 – 1512176Helicase ATP-binding 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1545 – 1753209Helicase C-terminal 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1812 – 2124313SEC63 2Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili54 – 8431Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi615 – 6184DEAH box
    Motifi1454 – 14574DEAH box

    Domaini

    Contains two helicase domains. The N-terminal helicase domain has catalytic activity by itself, contrary to C-terminal helicase domain that may have a regulatory role and enhance the activity of the first helicase domain.By similarity

    Sequence similaritiesi

    Belongs to the helicase family. SKI2 subfamily.Curated
    Contains 2 helicase ATP-binding domains.PROSITE-ProRule annotation
    Contains 2 helicase C-terminal domains.PROSITE-ProRule annotation
    Contains 2 SEC63 domains.Curated

    Keywords - Domaini

    Coiled coil, Repeat

    Phylogenomic databases

    GeneTreeiENSGT00640000091272.
    HOGENOMiHOG000157749.
    HOVERGENiHBG051896.
    InParanoidiQ6P4T2.
    KOiK12854.
    OMAiKKMENWW.
    OrthoDBiEOG7PGDPQ.
    PhylomeDBiQ6P4T2.
    TreeFamiTF300056.

    Family and domain databases

    Gene3Di2.60.40.150. 1 hit.
    3.40.50.300. 4 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR000008. C2_dom.
    IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR014756. Ig_E-set.
    IPR027417. P-loop_NTPase.
    IPR004179. Sec63-dom.
    [Graphical view]
    PfamiPF00270. DEAD. 2 hits.
    PF00271. Helicase_C. 2 hits.
    PF02889. Sec63. 2 hits.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    SM00487. DEXDc. 2 hits.
    SM00490. HELICc. 2 hits.
    SM00611. SEC63. 2 hits.
    SM00973. Sec63. 2 hits.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 4 hits.
    SSF81296. SSF81296. 1 hit.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 2 hits.
    PS51194. HELICASE_CTER. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6P4T2-1 [UniParc]FASTAAdd to Basket

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    MADVTARSLQ YEYKANSNLV LQADRSLIDR TRRDEPTGEV LSLVGKLEGT     50
    RMGDKAQRTK PQMQEERRAK RRKRDEDRHD MNKMKGYTLL SEGIDEMVGI 100
    IYKPKTKETR ETYEVLLSFI QAALGDQPRD ILCGAADEVL AVLKNEKLRD 150
    KERRREIDLL LGQTDDTRYH VLVNLGKKIT DYGGDKEIQN MDDNIDETYG 200
    VNVQFESDEE EGDEDVYGEV REEASDDDME GDEAVVRCTL SANLVASGEL 250
    MSSKKKDLHP RDIDAFWLQR QLSRFYDDAI VSQKKADEVL EILKTASDDR 300
    ECENQLVLLL GFNTFDFIKV LRQHRMMILY CTLLASAQSE PEKERIVGKM 350
    EADPELSKFL YQLHETEKED LIREERSRRE RVRQSRMDTD LETMDLDQGG 400
    EALAPRQVLD LEDLVFTQGS HFMANKRCQL PDGSFRRQRK GYEEVHVPAL 450
    KPKPFGSEEQ LLPVEKLPKY AQAGFEGFKT LNRIQSKLYR AALETDENLL 500
    LCAPTGAGKT NVALMCMLRE IGKHINMDGT INVDDFKIIY IAPMRSLVQE 550
    MVGSFGKRLA TYGITVAELT GDHQLCKEEI SATQIIVCTP EKWDIITRKG 600
    GERTYTQLVR LIVLDEIHLL HDDRGPVLEA LVARAIRNIE MTQEDVRLIG 650
    LSATLPNYED VATFLRVDPA KGLFYFDNSF RPVPLEQTYV GITEKKAIKR 700
    FQIMNEIVYE KIMEHAGKNQ VLVFVHSRKE TGKTARAIRD MCLEKDTLGL 750
    FLREGSASTE VLRTEAEQCK NLELKDLLPY GFAIHHAGMT RVDRTLVEDL 800
    FADKHIQVLV STATLAWGVN LPAHTVIIKG TQVYSPEKGR WTELGALDIL 850
    QMLGRAGRPQ YDTKGEGILI TSHGELQYYL SLLNQQLPIE SQMVSKLPDM 900
    LNAEIVLGNV QNAKDAVNWL GYAYLYIRML RSPTLYGISH DDLKGDPLLD 950
    QRRLDLVHTA ALMLDKNNLV KYDKKTGNFQ VTELGRIASH YYITNDTVQT 1000
    YNQLLKPTLS EIELFRVFSL SSEFKNITVR EEEKLELQKL LERVPIPVKE 1050
    SIEEPSAKIN VLLQAFISQL KLEGFALMAD MVYVTQSAGR LMRAIFEIVL 1100
    NRGWAQLTDK TLNLCKMIDK RMWQSMCPLR QFRKLPEEVV KKIEKKNFPF 1150
    ERLYDLNHNE IGELIRMPKM GKTIHKYVHL FPKLELSVHL QPITRSTLKV 1200
    ELTITPDFQW DEKVHGSSEA FWILVEDVDS EVILHHEYFL LKAKYAQDEH 1250
    LITFFVPVFE PLPPQYFIRV VSDRWLSCET QLPVSFRHLI LPEKYPPPTE 1300
    LLDLQPLPVS ALRNSAFESL YQDKFPFFNP IQTQVFNTVY NSDDNVFVGA 1350
    PTGSGKTICA EFAILRMLLQ NSEGRCVYIT PMEALAEQVY MDWYEKFQDR 1400
    LNKKVVLLTG ETSTDLKLLG KGNIIISTPE KWDILSRRWK QRKNVQNINL 1450
    FVVDEVHLIG GENGPVLEVI CSRMRYISSQ IERPIRIVAL SSSLSNAKDV 1500
    AHWLGCSATS TFNFHPNVRP VPLELHIQGF NISHTQTRLL SMAKPVYHAI 1550
    TKHSPKKPVI VFVPSRKQTR LTAIDILTTC AADIQRQRFL HCTEKDLIPY 1600
    LEKLSDSTLK ETLLNGVGYL HEGLSPMERR LVEQLFSSGA IQVVVASRSL 1650
    CWGMNVAAHL VIIMDTQYYN GKIHAYVDYP IYDVLQMVGH ANRPLQDDEG 1700
    RCVIMCQGSK KDFFKKFLYE PLPVESHLDH CMHDHFNAEI VTKTIENKQD 1750
    AVDYLTWTFL YRRMTQNPNY YNLQGISHRH LSDHLSELVE QTLSDLEQSK 1800
    CISIEDEMDV APLNLGMIAA YYYINYTTIE LFSMSLNAKT KVRGLIEIIS 1850
    NAAEYENIPI RHHEDNLLRQ LAQKVPHKLN NPKFNDPHVK TNLLLQAHLS 1900
    RMQLSAELQS DTEEILSKAI RLIQACVDVL SSNGWLSPAL AAMELAQMVT 1950
    QAMWSKDSYL KQLPHFTSEH IKRCTDKGVE SVFDIMEMED EERNALLQLT 2000
    DSQIADVARF CNRYPNIELS YEVVDKDSIR SGGPVVVLVQ LEREEEVTGP 2050
    VIAPLFPQKR EEGWWVVIGD AKSNSLISIK RLTLQQKAKV KLDFVAPATG 2100
    GHNYTLYFMS DAYMGCDQEY KFSVDVKEAE TDSDSD 2136
    Length:2,136
    Mass (Da):244,547
    Last modified:July 5, 2004 - v1
    Checksum:iBBDD6058CA635519
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL845368 Genomic DNA. No translation available.
    BC063261 mRNA. Translation: AAH63261.1.
    AK173025 mRNA. Translation: BAD32303.1.
    CCDSiCCDS16695.1.
    RefSeqiNP_796188.2. NM_177214.4.
    UniGeneiMm.215860.

    Genome annotation databases

    EnsembliENSMUST00000103220; ENSMUSP00000099509; ENSMUSG00000003660.
    GeneIDi320632.
    KEGGimmu:320632.
    UCSCiuc008mez.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL845368 Genomic DNA. No translation available.
    BC063261 mRNA. Translation: AAH63261.1 .
    AK173025 mRNA. Translation: BAD32303.1 .
    CCDSi CCDS16695.1.
    RefSeqi NP_796188.2. NM_177214.4.
    UniGenei Mm.215860.

    3D structure databases

    ProteinModelPortali Q6P4T2.
    SMRi Q6P4T2. Positions 1840-2129.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q6P4T2. 2 interactions.
    MINTi MINT-8174184.

    Proteomic databases

    MaxQBi Q6P4T2.
    PRIDEi Q6P4T2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000103220 ; ENSMUSP00000099509 ; ENSMUSG00000003660 .
    GeneIDi 320632.
    KEGGi mmu:320632.
    UCSCi uc008mez.1. mouse.

    Organism-specific databases

    CTDi 23020.
    MGIi MGI:2444401. Snrnp200.
    Rougei Search...

    Phylogenomic databases

    GeneTreei ENSGT00640000091272.
    HOGENOMi HOG000157749.
    HOVERGENi HBG051896.
    InParanoidi Q6P4T2.
    KOi K12854.
    OMAi KKMENWW.
    OrthoDBi EOG7PGDPQ.
    PhylomeDBi Q6P4T2.
    TreeFami TF300056.

    Miscellaneous databases

    ChiTaRSi SNRNP200. mouse.
    NextBioi 397101.
    PROi Q6P4T2.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori Q6P4T2.

    Family and domain databases

    Gene3Di 2.60.40.150. 1 hit.
    3.40.50.300. 4 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR000008. C2_dom.
    IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR014756. Ig_E-set.
    IPR027417. P-loop_NTPase.
    IPR004179. Sec63-dom.
    [Graphical view ]
    Pfami PF00270. DEAD. 2 hits.
    PF00271. Helicase_C. 2 hits.
    PF02889. Sec63. 2 hits.
    [Graphical view ]
    SMARTi SM00382. AAA. 2 hits.
    SM00487. DEXDc. 2 hits.
    SM00490. HELICc. 2 hits.
    SM00611. SEC63. 2 hits.
    SM00973. Sec63. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 4 hits.
    SSF81296. SSF81296. 1 hit.
    PROSITEi PS51192. HELICASE_ATP_BIND_1. 2 hits.
    PS51194. HELICASE_CTER. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    3. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
      DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 228-2136.
      Tissue: Embryonic tail.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1765, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    6. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
      Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
      J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    7. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiU520_MOUSE
    AccessioniPrimary (citable) accession number: Q6P4T2
    Secondary accession number(s): Q69ZZ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2013
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3