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Q6P4S6 (SIK3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase SIK3

EC=2.7.11.1
Alternative name(s):
Salt-inducible kinase 3
Short name=SIK-3
Serine/threonine-protein kinase QSK
Gene names
Name:Sik3
Synonyms:Kiaa0999, Qsk
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1311 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Enzyme regulation

Activated by phosphorylation on Thr-163 By similarity.

Subunit structure

Binds to and is activated by YWHAZ when phosphorylated on Thr-163 By similarity.

Subcellular location

Cytoplasm. Note: Locates to punctate structures within the cytoplasm on binding to YWHAZ By similarity.

Post-translational modification

Phosphorylated at Thr-163 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

Contains 1 protein kinase domain.

Contains 1 UBA domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6P4S6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6P4S6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-58: Missing.
     546-1311: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13111311Serine/threonine-protein kinase SIK3
PRO_0000252258

Regions

Domain8 – 259252Protein kinase
Domain286 – 32641UBA
Nucleotide binding14 – 229ATP By similarity
Compositional bias640 – 1003364Gln-rich

Sites

Active site1301Proton acceptor By similarity
Binding site371ATP By similarity

Amino acid modifications

Modified residue131Phosphothreonine By similarity
Modified residue1631Phosphothreonine By similarity
Modified residue4931Phosphoserine Ref.4
Modified residue5331Phosphoserine Ref.4
Modified residue5341Phosphoserine Ref.4
Modified residue6161Phosphoserine By similarity
Modified residue8561Phosphoserine By similarity

Natural variations

Alternative sequence1 – 5858Missing in isoform 2.
VSP_020894
Alternative sequence546 – 1311766Missing in isoform 2.
VSP_020895

Experimental info

Sequence conflict10321H → Q in AAH82313. Ref.2
Sequence conflict10321H → Q in BAC98067. Ref.3
Sequence conflict12771S → N in AAH63268. Ref.2
Sequence conflict12771S → N in AAH80688. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 3, 2006. Version 3.
Checksum: CF110B679B4C3F2B

FASTA1,311145,784
        10         20         30         40         50         60 
MAARIGYYEI DRTIGKGNFA VVKRATHLVT KAKVAIKIID KSQLDEENLK KIFREVQIMK 

        70         80         90        100        110        120 
MLCHPHIIRL YQVMETERMI YLVTEYASGG EIFDHLVAHG RMAEKEARRK FKQIVTAVYF 

       130        140        150        160        170        180 
CHCRNIVHRD LKAENLLLDA NLNIKIADFG FSNLFTPGQL LKTWCGSPPY AAPELFEGKE 

       190        200        210        220        230        240 
YDGPKVDIWS LGVVLYVLVC GALPFDGSTL QNLRARVLSG KFRIPFFMST ECEHLIRHML 

       250        260        270        280        290        300 
VLDPNKRLSM EQICRHKWMK LGDADPNFDR LIAECQQLKE ERQSDPLNDD VLLAMEDMGL 

       310        320        330        340        350        360 
DKERTLQSLR SDAYDHYSAI YSLLCDRHKK HKTLRPGALP SMPQAMTFQA PVNLQAEQTG 

       370        380        390        400        410        420 
TAMNLSVPQV QLINPENQII EPDGAVNLDS DEGEEPSPEA LVRYLSMRRH TVGVADPRTE 

       430        440        450        460        470        480 
VMEDLQKLLP GFPGVNPQGP FLQVAPNMNF THNLLPMQSL QPTGQLEYKE QSLLQPPTLQ 

       490        500        510        520        530        540 
LLNGMGPLGR RASDGGANIQ LHAQQLLKRP RGPSPLVTMT PAVPAVTPVD EESSDGEPDQ 

       550        560        570        580        590        600 
EAVQRYLANR SKRHTLAMTS PTAEIPPDLQ RQLGQQSFRS RVWPPHLVPD QHRSTYKDSN 

       610        620        630        640        650        660 
TLHLPTERFS PVRRFSDGAA SIQAFKAHLE KMGNSSSIKQ LQQECEQLQK MYGGQVDERT 

       670        680        690        700        710        720 
LEKTQQQHML YQQEQHHQIL QQQIQDSICP PQPSPPLQVA CENQPALLTH QLQRLRIQPS 

       730        740        750        760        770        780 
SPPPNHPSNH LFRQPSNSPP PVSSAMITSH GATSPSQFQG LPSHGAIFQQ QPENCSPPPS 

       790        800        810        820        830        840 
VALTCLGLQQ ASQSQPVTIQ LQEPVDMLSN MAGTAAGSAG RSIPISPSAS QIQIQHRASL 

       850        860        870        880        890        900 
MAPFSYGHRP LSKQLSADSA EAHSLNMNRF SPANYDQAHL HPHLFSDQSR GSPSSYSPST 

       910        920        930        940        950        960 
GVGFPPTQAL KVPPLDQFPT FPPSAQQQPP HYTTSALQQA LLSPTPPDYP RHQQVPHILQ 

       970        980        990       1000       1010       1020 
GLLSPRHSLT GHSDIRLPPA EFAQLIKRQQ QHRQQQQQQQ QQQEYHELFR HMNQGDAVSL 

      1030       1040       1050       1060       1070       1080 
APSLGGQNMT EHQALSYQNA DSYHRHHTSP QHILQIRAQD CISQGPSPTP THGYAHQPPL 

      1090       1100       1110       1120       1130       1140 
MHSESMEEDC LCEGLKEGFP DKSSSTLTKG CHNSPLLLCT SGPGDPEPLL GTVSQARELG 

      1150       1160       1170       1180       1190       1200 
IHPYGHQPTA TTFSRNKVPS RESVLGNCLE RSSPGQAMEL PDHNGLGYPV RPLVSEHLRS 

      1210       1220       1230       1240       1250       1260 
RTLQRHHTIQ NSDDAYVQLD TLPGMSLVAG KALSSARMSD AVLSQSSLMG SQQFQDEEDE 

      1270       1280       1290       1300       1310 
ECGVSLGHEH PGLGDGSQHL NSSRYPATCV TDIMLSHKHP EVSFSMEQAG V 

« Hide

Isoform 2 [UniParc].

Checksum: 5FB269344262D4F2
Show »

FASTA48754,776

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6, C57BL/6J and FVB/N.
Tissue: Brain, Limb, Salivary gland and Thymus.
[3]"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 260-1311 (ISOFORM 1).
Tissue: Fetal brain.
[4]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-493; SER-533 AND SER-534, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK017789 mRNA. Translation: BAB30934.1.
BC033915 mRNA. Translation: AAH33915.1.
BC063268 mRNA. Translation: AAH63268.2.
BC080688 mRNA. Translation: AAH80688.1.
BC082313 mRNA. Translation: AAH82313.1.
AK129257 mRNA. Translation: BAC98067.1.
RefSeqNP_081774.3. NM_027498.3.
UniGeneMm.219459.

3D structure databases

ProteinModelPortalQ6P4S6.
SMRQ6P4S6. Positions 2-330.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid214193. 3 interactions.
IntActQ6P4S6. 3 interactions.

PTM databases

PhosphoSiteQ6P4S6.

Proteomic databases

MaxQBQ6P4S6.
PaxDbQ6P4S6.
PRIDEQ6P4S6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID70661.
KEGGmmu:70661.
UCSCuc009pha.1. mouse. [Q6P4S6-2]

Organism-specific databases

CTD23387.
MGIMGI:2446296. Sik3.
RougeSearch...

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000154056.
HOVERGENHBG079682.
InParanoidQ6P4S6.
KOK16311.
PhylomeDBQ6P4S6.

Gene expression databases

CleanExMM_BC033915.
GenevestigatorQ6P4S6.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR015940. UBA/transl_elong_EF1B_N_euk.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 2 hits.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSIK3. mouse.
NextBio332051.
PROQ6P4S6.
SOURCESearch...

Entry information

Entry nameSIK3_MOUSE
AccessionPrimary (citable) accession number: Q6P4S6
Secondary accession number(s): Q641L5 expand/collapse secondary AC list , Q66JZ5, Q6ZQ09, Q8K075, Q9CYD5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 3, 2006
Last modified: June 11, 2014
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot