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Protein

Serine/threonine-protein kinase SIK3

Gene

Sik3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by phosphorylation on Thr-163.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37ATPPROSITE-ProRule annotation1
Active sitei130Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 22ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cartilage development involved in endochondral bone morphogenesis Source: MGI
  • endochondral ossification Source: MGI
  • limb morphogenesis Source: MGI
  • multicellular organism growth Source: MGI
  • protein phosphorylation Source: UniProtKB
  • regulation of glucose metabolic process Source: GO_Central
  • regulation of protein localization Source: MGI
  • skeletal system morphogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase SIK3 (EC:2.7.11.1)
Alternative name(s):
Salt-inducible kinase 3
Short name:
SIK-3
Serine/threonine-protein kinase QSK
Gene namesi
Name:Sik3
Synonyms:Kiaa0999, Qsk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2446296. Sik3.

Subcellular locationi

  • Cytoplasm

  • Note: Locates to punctate structures within the cytoplasm on binding to YWHAZ.By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002522581 – 1311Serine/threonine-protein kinase SIK3Add BLAST1311

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13PhosphothreonineBy similarity1
Modified residuei163PhosphothreonineCombined sources1
Modified residuei411PhosphothreonineBy similarity1
Modified residuei493PhosphoserineCombined sources1
Modified residuei533PhosphoserineCombined sources1
Modified residuei534PhosphoserineCombined sources1
Modified residuei616PhosphoserineCombined sources1
Modified residuei637PhosphoserineCombined sources1
Modified residuei856PhosphoserineBy similarity1
Modified residuei968PhosphoserineBy similarity1
Modified residuei976Omega-N-methylarginineCombined sources1

Post-translational modificationi

Phosphorylated at Thr-163 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ6P4S6.
PaxDbiQ6P4S6.
PeptideAtlasiQ6P4S6.
PRIDEiQ6P4S6.

PTM databases

iPTMnetiQ6P4S6.
PhosphoSitePlusiQ6P4S6.

Expressioni

Gene expression databases

CleanExiMM_BC033915.

Interactioni

Subunit structurei

Binds to and is activated by YWHAZ when phosphorylated on Thr-163.By similarity

Protein-protein interaction databases

BioGridi214193. 3 interactors.
IntActiQ6P4S6. 3 interactors.
STRINGi10090.ENSMUSP00000121032.

Structurei

3D structure databases

ProteinModelPortaliQ6P4S6.
SMRiQ6P4S6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 259Protein kinasePROSITE-ProRule annotationAdd BLAST252
Domaini286 – 326UBAPROSITE-ProRule annotationAdd BLAST41

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi640 – 1003Gln-richAdd BLAST364

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 UBA domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0586. Eukaryota.
ENOG410XNQ0. LUCA.
HOGENOMiHOG000154056.
HOVERGENiHBG079682.
InParanoidiQ6P4S6.
KOiK19009.
PhylomeDBiQ6P4S6.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR015940. UBA.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6P4S6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAARIGYYEI DRTIGKGNFA VVKRATHLVT KAKVAIKIID KSQLDEENLK
60 70 80 90 100
KIFREVQIMK MLCHPHIIRL YQVMETERMI YLVTEYASGG EIFDHLVAHG
110 120 130 140 150
RMAEKEARRK FKQIVTAVYF CHCRNIVHRD LKAENLLLDA NLNIKIADFG
160 170 180 190 200
FSNLFTPGQL LKTWCGSPPY AAPELFEGKE YDGPKVDIWS LGVVLYVLVC
210 220 230 240 250
GALPFDGSTL QNLRARVLSG KFRIPFFMST ECEHLIRHML VLDPNKRLSM
260 270 280 290 300
EQICRHKWMK LGDADPNFDR LIAECQQLKE ERQSDPLNDD VLLAMEDMGL
310 320 330 340 350
DKERTLQSLR SDAYDHYSAI YSLLCDRHKK HKTLRPGALP SMPQAMTFQA
360 370 380 390 400
PVNLQAEQTG TAMNLSVPQV QLINPENQII EPDGAVNLDS DEGEEPSPEA
410 420 430 440 450
LVRYLSMRRH TVGVADPRTE VMEDLQKLLP GFPGVNPQGP FLQVAPNMNF
460 470 480 490 500
THNLLPMQSL QPTGQLEYKE QSLLQPPTLQ LLNGMGPLGR RASDGGANIQ
510 520 530 540 550
LHAQQLLKRP RGPSPLVTMT PAVPAVTPVD EESSDGEPDQ EAVQRYLANR
560 570 580 590 600
SKRHTLAMTS PTAEIPPDLQ RQLGQQSFRS RVWPPHLVPD QHRSTYKDSN
610 620 630 640 650
TLHLPTERFS PVRRFSDGAA SIQAFKAHLE KMGNSSSIKQ LQQECEQLQK
660 670 680 690 700
MYGGQVDERT LEKTQQQHML YQQEQHHQIL QQQIQDSICP PQPSPPLQVA
710 720 730 740 750
CENQPALLTH QLQRLRIQPS SPPPNHPSNH LFRQPSNSPP PVSSAMITSH
760 770 780 790 800
GATSPSQFQG LPSHGAIFQQ QPENCSPPPS VALTCLGLQQ ASQSQPVTIQ
810 820 830 840 850
LQEPVDMLSN MAGTAAGSAG RSIPISPSAS QIQIQHRASL MAPFSYGHRP
860 870 880 890 900
LSKQLSADSA EAHSLNMNRF SPANYDQAHL HPHLFSDQSR GSPSSYSPST
910 920 930 940 950
GVGFPPTQAL KVPPLDQFPT FPPSAQQQPP HYTTSALQQA LLSPTPPDYP
960 970 980 990 1000
RHQQVPHILQ GLLSPRHSLT GHSDIRLPPA EFAQLIKRQQ QHRQQQQQQQ
1010 1020 1030 1040 1050
QQQEYHELFR HMNQGDAVSL APSLGGQNMT EHQALSYQNA DSYHRHHTSP
1060 1070 1080 1090 1100
QHILQIRAQD CISQGPSPTP THGYAHQPPL MHSESMEEDC LCEGLKEGFP
1110 1120 1130 1140 1150
DKSSSTLTKG CHNSPLLLCT SGPGDPEPLL GTVSQARELG IHPYGHQPTA
1160 1170 1180 1190 1200
TTFSRNKVPS RESVLGNCLE RSSPGQAMEL PDHNGLGYPV RPLVSEHLRS
1210 1220 1230 1240 1250
RTLQRHHTIQ NSDDAYVQLD TLPGMSLVAG KALSSARMSD AVLSQSSLMG
1260 1270 1280 1290 1300
SQQFQDEEDE ECGVSLGHEH PGLGDGSQHL NSSRYPATCV TDIMLSHKHP
1310
EVSFSMEQAG V
Length:1,311
Mass (Da):145,784
Last modified:October 3, 2006 - v3
Checksum:iCF110B679B4C3F2B
GO
Isoform 2 (identifier: Q6P4S6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-58: Missing.
     546-1311: Missing.

Note: No experimental confirmation available.
Show »
Length:487
Mass (Da):54,776
Checksum:i5FB269344262D4F2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1032H → Q in AAH82313 (PubMed:15489334).Curated1
Sequence conflicti1032H → Q in BAC98067 (PubMed:14621295).Curated1
Sequence conflicti1277S → N in AAH63268 (PubMed:15489334).Curated1
Sequence conflicti1277S → N in AAH80688 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0208941 – 58Missing in isoform 2. 1 PublicationAdd BLAST58
Alternative sequenceiVSP_020895546 – 1311Missing in isoform 2. 1 PublicationAdd BLAST766

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017789 mRNA. Translation: BAB30934.1.
BC033915 mRNA. Translation: AAH33915.1.
BC063268 mRNA. Translation: AAH63268.2.
BC080688 mRNA. Translation: AAH80688.1.
BC082313 mRNA. Translation: AAH82313.1.
AK129257 mRNA. Translation: BAC98067.1.
RefSeqiNP_081774.3. NM_027498.3.
UniGeneiMm.219459.

Genome annotation databases

GeneIDi70661.
KEGGimmu:70661.
UCSCiuc009pha.1. mouse. [Q6P4S6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017789 mRNA. Translation: BAB30934.1.
BC033915 mRNA. Translation: AAH33915.1.
BC063268 mRNA. Translation: AAH63268.2.
BC080688 mRNA. Translation: AAH80688.1.
BC082313 mRNA. Translation: AAH82313.1.
AK129257 mRNA. Translation: BAC98067.1.
RefSeqiNP_081774.3. NM_027498.3.
UniGeneiMm.219459.

3D structure databases

ProteinModelPortaliQ6P4S6.
SMRiQ6P4S6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214193. 3 interactors.
IntActiQ6P4S6. 3 interactors.
STRINGi10090.ENSMUSP00000121032.

PTM databases

iPTMnetiQ6P4S6.
PhosphoSitePlusiQ6P4S6.

Proteomic databases

EPDiQ6P4S6.
PaxDbiQ6P4S6.
PeptideAtlasiQ6P4S6.
PRIDEiQ6P4S6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi70661.
KEGGimmu:70661.
UCSCiuc009pha.1. mouse. [Q6P4S6-2]

Organism-specific databases

CTDi23387.
MGIiMGI:2446296. Sik3.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0586. Eukaryota.
ENOG410XNQ0. LUCA.
HOGENOMiHOG000154056.
HOVERGENiHBG079682.
InParanoidiQ6P4S6.
KOiK19009.
PhylomeDBiQ6P4S6.

Miscellaneous databases

ChiTaRSiSik3. mouse.
PROiQ6P4S6.
SOURCEiSearch...

Gene expression databases

CleanExiMM_BC033915.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR015940. UBA.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSIK3_MOUSE
AccessioniPrimary (citable) accession number: Q6P4S6
Secondary accession number(s): Q641L5
, Q66JZ5, Q6ZQ09, Q8K075, Q9CYD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 3, 2006
Last modified: November 2, 2016
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.