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Protein

Serine/threonine-protein kinase SIK3

Gene

Sik3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by phosphorylation on Thr-163.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei37 – 371ATPPROSITE-ProRule annotation
Active sitei130 – 1301Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 229ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. cartilage development involved in endochondral bone morphogenesis Source: MGI
  2. endochondral ossification Source: MGI
  3. limb morphogenesis Source: MGI
  4. multicellular organism growth Source: MGI
  5. protein phosphorylation Source: UniProtKB
  6. regulation of protein localization Source: MGI
  7. skeletal system morphogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase SIK3 (EC:2.7.11.1)
Alternative name(s):
Salt-inducible kinase 3
Short name:
SIK-3
Serine/threonine-protein kinase QSK
Gene namesi
Name:Sik3
Synonyms:Kiaa0999, Qsk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:2446296. Sik3.

Subcellular locationi

Cytoplasm
Note: Locates to punctate structures within the cytoplasm on binding to YWHAZ.By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13111311Serine/threonine-protein kinase SIK3PRO_0000252258Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei13 – 131PhosphothreonineBy similarity
Modified residuei163 – 1631PhosphothreonineBy similarity
Modified residuei411 – 4111PhosphothreonineBy similarity
Modified residuei493 – 4931Phosphoserine1 Publication
Modified residuei533 – 5331Phosphoserine1 Publication
Modified residuei534 – 5341Phosphoserine1 Publication
Modified residuei616 – 6161PhosphoserineBy similarity
Modified residuei856 – 8561PhosphoserineBy similarity
Modified residuei968 – 9681PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated at Thr-163 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6P4S6.
PaxDbiQ6P4S6.
PRIDEiQ6P4S6.

PTM databases

PhosphoSiteiQ6P4S6.

Expressioni

Gene expression databases

CleanExiMM_BC033915.
GenevestigatoriQ6P4S6.

Interactioni

Subunit structurei

Binds to and is activated by YWHAZ when phosphorylated on Thr-163.By similarity

Protein-protein interaction databases

BioGridi214193. 3 interactions.
IntActiQ6P4S6. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ6P4S6.
SMRiQ6P4S6. Positions 5-330.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 259252Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini286 – 32641UBAPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi640 – 1003364Gln-richAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 UBA domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000154056.
HOVERGENiHBG079682.
InParanoidiQ6P4S6.
KOiK16311.
PhylomeDBiQ6P4S6.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
IPR015940. UBA/transl_elong_EF1B_N_euk.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6P4S6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAARIGYYEI DRTIGKGNFA VVKRATHLVT KAKVAIKIID KSQLDEENLK
60 70 80 90 100
KIFREVQIMK MLCHPHIIRL YQVMETERMI YLVTEYASGG EIFDHLVAHG
110 120 130 140 150
RMAEKEARRK FKQIVTAVYF CHCRNIVHRD LKAENLLLDA NLNIKIADFG
160 170 180 190 200
FSNLFTPGQL LKTWCGSPPY AAPELFEGKE YDGPKVDIWS LGVVLYVLVC
210 220 230 240 250
GALPFDGSTL QNLRARVLSG KFRIPFFMST ECEHLIRHML VLDPNKRLSM
260 270 280 290 300
EQICRHKWMK LGDADPNFDR LIAECQQLKE ERQSDPLNDD VLLAMEDMGL
310 320 330 340 350
DKERTLQSLR SDAYDHYSAI YSLLCDRHKK HKTLRPGALP SMPQAMTFQA
360 370 380 390 400
PVNLQAEQTG TAMNLSVPQV QLINPENQII EPDGAVNLDS DEGEEPSPEA
410 420 430 440 450
LVRYLSMRRH TVGVADPRTE VMEDLQKLLP GFPGVNPQGP FLQVAPNMNF
460 470 480 490 500
THNLLPMQSL QPTGQLEYKE QSLLQPPTLQ LLNGMGPLGR RASDGGANIQ
510 520 530 540 550
LHAQQLLKRP RGPSPLVTMT PAVPAVTPVD EESSDGEPDQ EAVQRYLANR
560 570 580 590 600
SKRHTLAMTS PTAEIPPDLQ RQLGQQSFRS RVWPPHLVPD QHRSTYKDSN
610 620 630 640 650
TLHLPTERFS PVRRFSDGAA SIQAFKAHLE KMGNSSSIKQ LQQECEQLQK
660 670 680 690 700
MYGGQVDERT LEKTQQQHML YQQEQHHQIL QQQIQDSICP PQPSPPLQVA
710 720 730 740 750
CENQPALLTH QLQRLRIQPS SPPPNHPSNH LFRQPSNSPP PVSSAMITSH
760 770 780 790 800
GATSPSQFQG LPSHGAIFQQ QPENCSPPPS VALTCLGLQQ ASQSQPVTIQ
810 820 830 840 850
LQEPVDMLSN MAGTAAGSAG RSIPISPSAS QIQIQHRASL MAPFSYGHRP
860 870 880 890 900
LSKQLSADSA EAHSLNMNRF SPANYDQAHL HPHLFSDQSR GSPSSYSPST
910 920 930 940 950
GVGFPPTQAL KVPPLDQFPT FPPSAQQQPP HYTTSALQQA LLSPTPPDYP
960 970 980 990 1000
RHQQVPHILQ GLLSPRHSLT GHSDIRLPPA EFAQLIKRQQ QHRQQQQQQQ
1010 1020 1030 1040 1050
QQQEYHELFR HMNQGDAVSL APSLGGQNMT EHQALSYQNA DSYHRHHTSP
1060 1070 1080 1090 1100
QHILQIRAQD CISQGPSPTP THGYAHQPPL MHSESMEEDC LCEGLKEGFP
1110 1120 1130 1140 1150
DKSSSTLTKG CHNSPLLLCT SGPGDPEPLL GTVSQARELG IHPYGHQPTA
1160 1170 1180 1190 1200
TTFSRNKVPS RESVLGNCLE RSSPGQAMEL PDHNGLGYPV RPLVSEHLRS
1210 1220 1230 1240 1250
RTLQRHHTIQ NSDDAYVQLD TLPGMSLVAG KALSSARMSD AVLSQSSLMG
1260 1270 1280 1290 1300
SQQFQDEEDE ECGVSLGHEH PGLGDGSQHL NSSRYPATCV TDIMLSHKHP
1310
EVSFSMEQAG V
Length:1,311
Mass (Da):145,784
Last modified:October 3, 2006 - v3
Checksum:iCF110B679B4C3F2B
GO
Isoform 2 (identifier: Q6P4S6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-58: Missing.
     546-1311: Missing.

Note: No experimental confirmation available.

Show »
Length:487
Mass (Da):54,776
Checksum:i5FB269344262D4F2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1032 – 10321H → Q in AAH82313 (PubMed:15489334).Curated
Sequence conflicti1032 – 10321H → Q in BAC98067 (PubMed:14621295).Curated
Sequence conflicti1277 – 12771S → N in AAH63268 (PubMed:15489334).Curated
Sequence conflicti1277 – 12771S → N in AAH80688 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5858Missing in isoform 2. 1 PublicationVSP_020894Add
BLAST
Alternative sequencei546 – 1311766Missing in isoform 2. 1 PublicationVSP_020895Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017789 mRNA. Translation: BAB30934.1.
BC033915 mRNA. Translation: AAH33915.1.
BC063268 mRNA. Translation: AAH63268.2.
BC080688 mRNA. Translation: AAH80688.1.
BC082313 mRNA. Translation: AAH82313.1.
AK129257 mRNA. Translation: BAC98067.1.
RefSeqiNP_081774.3. NM_027498.3.
UniGeneiMm.219459.

Genome annotation databases

GeneIDi70661.
KEGGimmu:70661.
UCSCiuc009pha.1. mouse. [Q6P4S6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017789 mRNA. Translation: BAB30934.1.
BC033915 mRNA. Translation: AAH33915.1.
BC063268 mRNA. Translation: AAH63268.2.
BC080688 mRNA. Translation: AAH80688.1.
BC082313 mRNA. Translation: AAH82313.1.
AK129257 mRNA. Translation: BAC98067.1.
RefSeqiNP_081774.3. NM_027498.3.
UniGeneiMm.219459.

3D structure databases

ProteinModelPortaliQ6P4S6.
SMRiQ6P4S6. Positions 5-330.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214193. 3 interactions.
IntActiQ6P4S6. 3 interactions.

PTM databases

PhosphoSiteiQ6P4S6.

Proteomic databases

MaxQBiQ6P4S6.
PaxDbiQ6P4S6.
PRIDEiQ6P4S6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi70661.
KEGGimmu:70661.
UCSCiuc009pha.1. mouse. [Q6P4S6-2]

Organism-specific databases

CTDi23387.
MGIiMGI:2446296. Sik3.
RougeiSearch...

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000154056.
HOVERGENiHBG079682.
InParanoidiQ6P4S6.
KOiK16311.
PhylomeDBiQ6P4S6.

Miscellaneous databases

ChiTaRSiSik3. mouse.
NextBioi332051.
PROiQ6P4S6.
SOURCEiSearch...

Gene expression databases

CleanExiMM_BC033915.
GenevestigatoriQ6P4S6.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
IPR015940. UBA/transl_elong_EF1B_N_euk.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6, C57BL/6J and FVB/N.
    Tissue: Brain, Limb, Salivary gland and Thymus.
  3. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 260-1311 (ISOFORM 1).
    Tissue: Fetal brain.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-493; SER-533 AND SER-534, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiSIK3_MOUSE
AccessioniPrimary (citable) accession number: Q6P4S6
Secondary accession number(s): Q641L5
, Q66JZ5, Q6ZQ09, Q8K075, Q9CYD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 3, 2006
Last modified: March 4, 2015
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.