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Q6P4S6

- SIK3_MOUSE

UniProt

Q6P4S6 - SIK3_MOUSE

Protein

Serine/threonine-protein kinase SIK3

Gene

Sik3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 3 (03 Oct 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by phosphorylation on Thr-163.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei37 – 371ATPPROSITE-ProRule annotation
    Active sitei130 – 1301Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi14 – 229ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. magnesium ion binding Source: UniProtKB
    3. protein binding Source: MGI
    4. protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    1. cartilage development involved in endochondral bone morphogenesis Source: MGI
    2. endochondral ossification Source: MGI
    3. limb morphogenesis Source: MGI
    4. multicellular organism growth Source: MGI
    5. protein phosphorylation Source: UniProtKB
    6. regulation of protein localization Source: MGI
    7. skeletal system morphogenesis Source: MGI

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase SIK3 (EC:2.7.11.1)
    Alternative name(s):
    Salt-inducible kinase 3
    Short name:
    SIK-3
    Serine/threonine-protein kinase QSK
    Gene namesi
    Name:Sik3
    Synonyms:Kiaa0999, Qsk
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:2446296. Sik3.

    Subcellular locationi

    Cytoplasm
    Note: Locates to punctate structures within the cytoplasm on binding to YWHAZ.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: MGI

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13111311Serine/threonine-protein kinase SIK3PRO_0000252258Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei13 – 131PhosphothreonineBy similarity
    Modified residuei163 – 1631PhosphothreonineBy similarity
    Modified residuei493 – 4931Phosphoserine1 Publication
    Modified residuei533 – 5331Phosphoserine1 Publication
    Modified residuei534 – 5341Phosphoserine1 Publication
    Modified residuei616 – 6161PhosphoserineBy similarity
    Modified residuei856 – 8561PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated at Thr-163 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ6P4S6.
    PaxDbiQ6P4S6.
    PRIDEiQ6P4S6.

    PTM databases

    PhosphoSiteiQ6P4S6.

    Expressioni

    Gene expression databases

    CleanExiMM_BC033915.
    GenevestigatoriQ6P4S6.

    Interactioni

    Subunit structurei

    Binds to and is activated by YWHAZ when phosphorylated on Thr-163.By similarity

    Protein-protein interaction databases

    BioGridi214193. 3 interactions.
    IntActiQ6P4S6. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6P4S6.
    SMRiQ6P4S6. Positions 2-330.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini8 – 259252Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini286 – 32641UBAPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi640 – 1003364Gln-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 UBA domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000154056.
    HOVERGENiHBG079682.
    InParanoidiQ6P4S6.
    KOiK16311.
    PhylomeDBiQ6P4S6.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 2 hits.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50030. UBA. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6P4S6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAARIGYYEI DRTIGKGNFA VVKRATHLVT KAKVAIKIID KSQLDEENLK     50
    KIFREVQIMK MLCHPHIIRL YQVMETERMI YLVTEYASGG EIFDHLVAHG 100
    RMAEKEARRK FKQIVTAVYF CHCRNIVHRD LKAENLLLDA NLNIKIADFG 150
    FSNLFTPGQL LKTWCGSPPY AAPELFEGKE YDGPKVDIWS LGVVLYVLVC 200
    GALPFDGSTL QNLRARVLSG KFRIPFFMST ECEHLIRHML VLDPNKRLSM 250
    EQICRHKWMK LGDADPNFDR LIAECQQLKE ERQSDPLNDD VLLAMEDMGL 300
    DKERTLQSLR SDAYDHYSAI YSLLCDRHKK HKTLRPGALP SMPQAMTFQA 350
    PVNLQAEQTG TAMNLSVPQV QLINPENQII EPDGAVNLDS DEGEEPSPEA 400
    LVRYLSMRRH TVGVADPRTE VMEDLQKLLP GFPGVNPQGP FLQVAPNMNF 450
    THNLLPMQSL QPTGQLEYKE QSLLQPPTLQ LLNGMGPLGR RASDGGANIQ 500
    LHAQQLLKRP RGPSPLVTMT PAVPAVTPVD EESSDGEPDQ EAVQRYLANR 550
    SKRHTLAMTS PTAEIPPDLQ RQLGQQSFRS RVWPPHLVPD QHRSTYKDSN 600
    TLHLPTERFS PVRRFSDGAA SIQAFKAHLE KMGNSSSIKQ LQQECEQLQK 650
    MYGGQVDERT LEKTQQQHML YQQEQHHQIL QQQIQDSICP PQPSPPLQVA 700
    CENQPALLTH QLQRLRIQPS SPPPNHPSNH LFRQPSNSPP PVSSAMITSH 750
    GATSPSQFQG LPSHGAIFQQ QPENCSPPPS VALTCLGLQQ ASQSQPVTIQ 800
    LQEPVDMLSN MAGTAAGSAG RSIPISPSAS QIQIQHRASL MAPFSYGHRP 850
    LSKQLSADSA EAHSLNMNRF SPANYDQAHL HPHLFSDQSR GSPSSYSPST 900
    GVGFPPTQAL KVPPLDQFPT FPPSAQQQPP HYTTSALQQA LLSPTPPDYP 950
    RHQQVPHILQ GLLSPRHSLT GHSDIRLPPA EFAQLIKRQQ QHRQQQQQQQ 1000
    QQQEYHELFR HMNQGDAVSL APSLGGQNMT EHQALSYQNA DSYHRHHTSP 1050
    QHILQIRAQD CISQGPSPTP THGYAHQPPL MHSESMEEDC LCEGLKEGFP 1100
    DKSSSTLTKG CHNSPLLLCT SGPGDPEPLL GTVSQARELG IHPYGHQPTA 1150
    TTFSRNKVPS RESVLGNCLE RSSPGQAMEL PDHNGLGYPV RPLVSEHLRS 1200
    RTLQRHHTIQ NSDDAYVQLD TLPGMSLVAG KALSSARMSD AVLSQSSLMG 1250
    SQQFQDEEDE ECGVSLGHEH PGLGDGSQHL NSSRYPATCV TDIMLSHKHP 1300
    EVSFSMEQAG V 1311
    Length:1,311
    Mass (Da):145,784
    Last modified:October 3, 2006 - v3
    Checksum:iCF110B679B4C3F2B
    GO
    Isoform 2 (identifier: Q6P4S6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-58: Missing.
         546-1311: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:487
    Mass (Da):54,776
    Checksum:i5FB269344262D4F2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1032 – 10321H → Q in AAH82313. (PubMed:15489334)Curated
    Sequence conflicti1032 – 10321H → Q in BAC98067. (PubMed:14621295)Curated
    Sequence conflicti1277 – 12771S → N in AAH63268. (PubMed:15489334)Curated
    Sequence conflicti1277 – 12771S → N in AAH80688. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 5858Missing in isoform 2. 1 PublicationVSP_020894Add
    BLAST
    Alternative sequencei546 – 1311766Missing in isoform 2. 1 PublicationVSP_020895Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK017789 mRNA. Translation: BAB30934.1.
    BC033915 mRNA. Translation: AAH33915.1.
    BC063268 mRNA. Translation: AAH63268.2.
    BC080688 mRNA. Translation: AAH80688.1.
    BC082313 mRNA. Translation: AAH82313.1.
    AK129257 mRNA. Translation: BAC98067.1.
    RefSeqiNP_081774.3. NM_027498.3.
    UniGeneiMm.219459.

    Genome annotation databases

    GeneIDi70661.
    KEGGimmu:70661.
    UCSCiuc009pha.1. mouse. [Q6P4S6-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK017789 mRNA. Translation: BAB30934.1 .
    BC033915 mRNA. Translation: AAH33915.1 .
    BC063268 mRNA. Translation: AAH63268.2 .
    BC080688 mRNA. Translation: AAH80688.1 .
    BC082313 mRNA. Translation: AAH82313.1 .
    AK129257 mRNA. Translation: BAC98067.1 .
    RefSeqi NP_081774.3. NM_027498.3.
    UniGenei Mm.219459.

    3D structure databases

    ProteinModelPortali Q6P4S6.
    SMRi Q6P4S6. Positions 2-330.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 214193. 3 interactions.
    IntActi Q6P4S6. 3 interactions.

    PTM databases

    PhosphoSitei Q6P4S6.

    Proteomic databases

    MaxQBi Q6P4S6.
    PaxDbi Q6P4S6.
    PRIDEi Q6P4S6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 70661.
    KEGGi mmu:70661.
    UCSCi uc009pha.1. mouse. [Q6P4S6-2 ]

    Organism-specific databases

    CTDi 23387.
    MGIi MGI:2446296. Sik3.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000154056.
    HOVERGENi HBG079682.
    InParanoidi Q6P4S6.
    KOi K16311.
    PhylomeDBi Q6P4S6.

    Miscellaneous databases

    ChiTaRSi SIK3. mouse.
    NextBioi 332051.
    PROi Q6P4S6.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_BC033915.
    Genevestigatori Q6P4S6.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 2 hits.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50030. UBA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6, C57BL/6J and FVB/N.
      Tissue: Brain, Limb, Salivary gland and Thymus.
    3. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
      DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 260-1311 (ISOFORM 1).
      Tissue: Fetal brain.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-493; SER-533 AND SER-534, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiSIK3_MOUSE
    AccessioniPrimary (citable) accession number: Q6P4S6
    Secondary accession number(s): Q641L5
    , Q66JZ5, Q6ZQ09, Q8K075, Q9CYD5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2006
    Last sequence update: October 3, 2006
    Last modified: October 1, 2014
    This is version 94 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3