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Protein

Phospholipase B-like 1

Gene

PLBD1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In view of the small size of the putative binding pocket, it has been proposed that it may act as an amidase or a peptidase (By similarity). Exhibits a weak phospholipase activity, acting on various phospholipids, including phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine and lysophospholipids.By similarity1 Publication

pH dependencei

Optimum pH is 7.4.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.1.5. 2681.
ReactomeiR-HSA-1482788. Acyl chain remodelling of PC.
R-HSA-1482839. Acyl chain remodelling of PE.
R-HSA-1482922. Acyl chain remodelling of PI.
R-HSA-1483115. Hydrolysis of LPC.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase B-like 1 (EC:3.1.1.-)
Alternative name(s):
LAMA-like protein 1
Lamina ancestor homolog 1
Phospholipase B domain-containing protein 1
Cleaved into the following 3 chains:
Gene namesi
Name:PLBD1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:26215. PLBD1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • extracellular space Source: UniProtKB
  • lysosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi79887.
OpenTargetsiENSG00000121316.
PharmGKBiPA164724597.

Polymorphism and mutation databases

BioMutaiPLBD1.
DMDMi269849630.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 38Sequence analysisAdd BLAST38
ChainiPRO_000028610639 – 553Phospholipase B-like 1Add BLAST515
ChainiPRO_000042542139 – 208Phospholipase B-like 1 chain AAdd BLAST170
ChainiPRO_000042542293 – 208Phospholipase B-like 1 chain CAdd BLAST116
PropeptideiPRO_0000425423209 – 227Removed in mature formBy similarityAdd BLAST19
ChainiPRO_0000425424228 – 553Phospholipase B-like 1 chain BAdd BLAST326

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi71N-linked (GlcNAc...) (high mannose); alternateBy similarity1
Glycosylationi71N-linked (GlcNAc...) (hybrid); alternateBy similarity1
Glycosylationi308N-linked (GlcNAc...) (high mannose); alternateBy similarity1
Glycosylationi308N-linked (GlcNAc...) (hybrid); alternateBy similarity1
Glycosylationi366N-linked (GlcNAc...) (high mannose); alternateBy similarity1
Glycosylationi366N-linked (GlcNAc...) (hybrid); alternateBy similarity1
Glycosylationi411N-linked (GlcNAc...)1 Publication1
Disulfide bondi470 ↔ 475By similarity
Disulfide bondi474 ↔ 489By similarity
Glycosylationi526N-linked (GlcNAc...) (high mannose); alternateBy similarity1
Glycosylationi526N-linked (GlcNAc...) (hybrid); alternateBy similarity1

Post-translational modificationi

The maturation cleavages that produces chains A and B are required to open the putative substrate binding pocket. Both chains A and B remain associated in the mature protein.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ6P4A8.
MaxQBiQ6P4A8.
PaxDbiQ6P4A8.
PeptideAtlasiQ6P4A8.
PRIDEiQ6P4A8.

PTM databases

iPTMnetiQ6P4A8.
PhosphoSitePlusiQ6P4A8.

Expressioni

Tissue specificityi

Expressed in neutrophils and monocytes.1 Publication

Gene expression databases

BgeeiENSG00000121316.
ExpressionAtlasiQ6P4A8. baseline and differential.
GenevisibleiQ6P4A8. HS.

Organism-specific databases

HPAiHPA040303.

Interactioni

Subunit structurei

May form a homodimer, each monomer is composed of a chain A and a chain B.1 Publication

Protein-protein interaction databases

BioGridi122972. 13 interactors.
IntActiQ6P4A8. 2 interactors.
STRINGi9606.ENSP00000240617.

Structurei

3D structure databases

ProteinModelPortaliQ6P4A8.
SMRiQ6P4A8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phospholipase B-like family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3774. Eukaryota.
ENOG410XQRV. LUCA.
GeneTreeiENSGT00530000063509.
HOGENOMiHOG000006903.
HOVERGENiHBG108264.
InParanoidiQ6P4A8.
OMAiCCREDLN.
OrthoDBiEOG091G0G9H.
PhylomeDBiQ6P4A8.
TreeFamiTF315042.

Family and domain databases

InterProiIPR007000. PLipase_B-like.
[Graphical view]
PANTHERiPTHR12370. PTHR12370. 1 hit.
PfamiPF04916. Phospholip_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6P4A8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRGGPGGRP GLPQPPPLLL LLLLLPLLLV TAEPPKPAGV YYATAYWMPA
60 70 80 90 100
EKTVQVKNVM DKNGDAYGFY NNSVKTTGWG ILEIRAGYGS QTLSNEIIMF
110 120 130 140 150
VAGFLEGYLT APHMNDHYTN LYPQLITKPS IMDKVQDFME KQDKWTRKNI
160 170 180 190 200
KEYKTDSFWR HTGYVMAQID GLYVGAKKRA ILEGTKPMTL FQIQFLNSVG
210 220 230 240 250
DLLDLIPSLS PTKNGSLKVF KRWDMGHCSA LIKVLPGFEN ILFAHSSWYT
260 270 280 290 300
YAAMLRIYKH WDFNVIDKDT SSSRLSFSSY PGFLESLDDF YILSSGLILL
310 320 330 340 350
QTTNSVFNKT LLKQVIPETL LSWQRVRVAN MMADSGKRWA DIFSKYNSGT
360 370 380 390 400
YNNQYMVLDL KKVKLNHSLD KGTLYIVEQI PTYVEYSEQT DVLRKGYWPS
410 420 430 440 450
YNVPFHEKIY NWSGYPLLVQ KLGLDYSYDL APRAKIFRRD QGKVTDTASM
460 470 480 490 500
KYIMRYNNYK KDPYSRGDPC NTICCREDLN SPNPSPGGCY DTKVADIYLA
510 520 530 540 550
SQYTSYAISG PTVQGGLPVF RWDRFNKTLH QGMPEVYNFD FITMKPILKL

DIK
Length:553
Mass (Da):63,255
Last modified:November 24, 2009 - v2
Checksum:iB4258DD2E1A16FE0
GO

Sequence cautioni

The sequence AAH00909 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB15442 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18Missing in BAF83603 (PubMed:14702039).Curated1
Sequence conflicti18Missing in AAH63561 (PubMed:15489334).Curated1
Sequence conflicti241I → V in BAB15442 (PubMed:15489334).Curated1
Sequence conflicti302T → A in BAF83603 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_032072265V → I.2 PublicationsCorresponds to variant rs7957558dbSNPEnsembl.1
Natural variantiVAR_032073377V → A.Corresponds to variant rs2287541dbSNPEnsembl.1
Natural variantiVAR_032074534P → A.1 PublicationCorresponds to variant rs1600dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK026315 mRNA. Translation: BAB15442.1. Different initiation.
AK290914 mRNA. Translation: BAF83603.1.
AC008114 Genomic DNA. No translation available.
BC000909 mRNA. Translation: AAH00909.2. Different initiation.
BC063561 mRNA. Translation: AAH63561.1.
CCDSiCCDS31751.1.
RefSeqiNP_079105.4. NM_024829.5.
UniGeneiHs.131933.

Genome annotation databases

EnsembliENST00000240617; ENSP00000240617; ENSG00000121316.
GeneIDi79887.
KEGGihsa:79887.
UCSCiuc001rcc.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK026315 mRNA. Translation: BAB15442.1. Different initiation.
AK290914 mRNA. Translation: BAF83603.1.
AC008114 Genomic DNA. No translation available.
BC000909 mRNA. Translation: AAH00909.2. Different initiation.
BC063561 mRNA. Translation: AAH63561.1.
CCDSiCCDS31751.1.
RefSeqiNP_079105.4. NM_024829.5.
UniGeneiHs.131933.

3D structure databases

ProteinModelPortaliQ6P4A8.
SMRiQ6P4A8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122972. 13 interactors.
IntActiQ6P4A8. 2 interactors.
STRINGi9606.ENSP00000240617.

PTM databases

iPTMnetiQ6P4A8.
PhosphoSitePlusiQ6P4A8.

Polymorphism and mutation databases

BioMutaiPLBD1.
DMDMi269849630.

Proteomic databases

EPDiQ6P4A8.
MaxQBiQ6P4A8.
PaxDbiQ6P4A8.
PeptideAtlasiQ6P4A8.
PRIDEiQ6P4A8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000240617; ENSP00000240617; ENSG00000121316.
GeneIDi79887.
KEGGihsa:79887.
UCSCiuc001rcc.2. human.

Organism-specific databases

CTDi79887.
DisGeNETi79887.
GeneCardsiPLBD1.
H-InvDBHIX0010454.
HGNCiHGNC:26215. PLBD1.
HPAiHPA040303.
neXtProtiNX_Q6P4A8.
OpenTargetsiENSG00000121316.
PharmGKBiPA164724597.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3774. Eukaryota.
ENOG410XQRV. LUCA.
GeneTreeiENSGT00530000063509.
HOGENOMiHOG000006903.
HOVERGENiHBG108264.
InParanoidiQ6P4A8.
OMAiCCREDLN.
OrthoDBiEOG091G0G9H.
PhylomeDBiQ6P4A8.
TreeFamiTF315042.

Enzyme and pathway databases

BRENDAi3.1.1.5. 2681.
ReactomeiR-HSA-1482788. Acyl chain remodelling of PC.
R-HSA-1482839. Acyl chain remodelling of PE.
R-HSA-1482922. Acyl chain remodelling of PI.
R-HSA-1483115. Hydrolysis of LPC.

Miscellaneous databases

GenomeRNAii79887.
PROiQ6P4A8.

Gene expression databases

BgeeiENSG00000121316.
ExpressionAtlasiQ6P4A8. baseline and differential.
GenevisibleiQ6P4A8. HS.

Family and domain databases

InterProiIPR007000. PLipase_B-like.
[Graphical view]
PANTHERiPTHR12370. PTHR12370. 1 hit.
PfamiPF04916. Phospholip_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLBL1_HUMAN
AccessioniPrimary (citable) accession number: Q6P4A8
Secondary accession number(s): A8K4E9, Q9BVV3, Q9H625
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: November 24, 2009
Last modified: November 30, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.