Q6P499 (NPAL3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NIPA-like protein 3

Gene names

Name:	NIPAL3
Synonyms:	NPAL3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the NIPA family.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	magnesium ion transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6P499-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6P499-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-82: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q6P499-3)

The sequence of this isoform differs from the canonical sequence as follows:
     213-226: GSMTVVTVKAVAGM → VLLCHPGWSAVVQS
     227-406: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	NIPA-like protein 3		PRO_0000242150
Regions
	Transmembrane	33 – 53	21	Helical; Potential
	Transmembrane	76 – 96	21	Helical; Potential
	Transmembrane	101 – 121	21	Helical; Potential
	Transmembrane	135 – 155	21	Helical; Potential
	Transmembrane	171 – 191	21	Helical; Potential
	Transmembrane	202 – 222	21	Helical; Potential
	Transmembrane	240 – 260	21	Helical; Potential
	Transmembrane	271 – 291	21	Helical; Potential
	Transmembrane	300 – 320	21	Helical; Potential
	Compositional bias	18 – 21	4	Poly-Ser
Amino acid modifications
	Modified residue	372	1	Phosphoserine Ref.6
	Glycosylation	166	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 82	82	Missing in isoform 2.		VSP_019445
	Alternative sequence	213 – 226	14	GSMTV…AVAGM → VLLCHPGWSAVVQS in isoform 3.		VSP_019446
	Alternative sequence	227 – 406	180	Missing in isoform 3.		VSP_019447

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 42E87B84B01E7C88	FASTA	406	44,742
	10 20 30 40 50 60 MDGSHSAALK LQQLPPTSSS SAVSEASFSY KENLIGALLA IFGHLVVSIA LNLQKYCHIR 70 80 90 100 110 120 LAGSKDPRAY FKTKTWWLGL FLMLLGELGV FASYAFAPLS LIVPLSAVSV IASAIIGIIF 130 140 150 160 170 180 IKEKWKPKDF LRRYVLSFVG CGLAVVGTYL LVTFAPNSHE KMTGENVTRH LVSWPFLLYM 190 200 210 220 230 240 LVEIILFCLL LYFYKEKNAN NIVVILLLVA LLGSMTVVTV KAVAGMLVLS IQGNLQLDYP 250 260 270 280 290 300 IFYVMFVCMV ATAVYQAAFL SQASQMYDSS LIASVGYILS TTIAITAGAI FYLDFIGEDV 310 320 330 340 350 360 LHICMFALGC LIAFLGVFLI TRNRKKPIPF EPYISMDAMP GMQNMHDKGM TVQPELKASF 370 380 390 400 SYGALENNDN ISEIYAPATL PVMQEEHGSR SASGVPYRVL EHTKKE
	Isoform 2 [UniParc]. Checksum: D6F51AF9CA50C347	FASTA	324	35,764
	10 20 30 40 50 60 MLLGELGVFA SYAFAPLSLI VPLSAVSVIA SAIIGIIFIK EKWKPKDFLR RYVLSFVGCG 70 80 90 100 110 120 LAVVGTYLLV TFAPNSHEKM TGENVTRHLV SWPFLLYMLV EIILFCLLLY FYKEKNANNI 130 140 150 160 170 180 VVILLLVALL GSMTVVTVKA VAGMLVLSIQ GNLQLDYPIF YVMFVCMVAT AVYQAAFLSQ 190 200 210 220 230 240 ASQMYDSSLI ASVGYILSTT IAITAGAIFY LDFIGEDVLH ICMFALGCLI AFLGVFLITR 250 260 270 280 290 300 NRKKPIPFEP YISMDAMPGM QNMHDKGMTV QPELKASFSY GALENNDNIS EIYAPATLPV 310 320 MQEEHGSRSA SGVPYRVLEH TKKE
	Isoform 3 [UniParc]. Checksum: DE2A2780573E9AE0	FASTA	226	24,994
	10 20 30 40 50 60 MDGSHSAALK LQQLPPTSSS SAVSEASFSY KENLIGALLA IFGHLVVSIA LNLQKYCHIR 70 80 90 100 110 120 LAGSKDPRAY FKTKTWWLGL FLMLLGELGV FASYAFAPLS LIVPLSAVSV IASAIIGIIF 130 140 150 160 170 180 IKEKWKPKDF LRRYVLSFVG CGLAVVGTYL LVTFAPNSHE KMTGENVTRH LVSWPFLLYM 190 200 210 220 LVEIILFCLL LYFYKEKNAN NIVVILLLVA LLVLLCHPGW SAVVQS

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Endometrium.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Lymph and Placenta.
[6]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-372, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK312433 mRNA. Translation: BAG35342.1. BX640883 mRNA. Translation: CAE45938.1. AL031431 Genomic DNA. Translation: CAB48885.1. AL031431 Genomic DNA. Translation: CAI21413.1. AL031431 Genomic DNA. Translation: CAI21412.1. CH471134 Genomic DNA. Translation: EAW95130.1. BC001265 mRNA. Translation: AAH01265.1. BC063583 mRNA. Translation: AAH63583.1.
IPI	IPI00411353. IPI00761050. IPI00783575.
RefSeq	NP_065181.1. NM_020448.4.
UniGene	Hs.523442.
3D structure databases
ProteinModelPortal	Q6P499.
ModBase	Search...
Protein family/group databases
TCDB	2.A.7.25.3. drug/metabolite transporter (DMT) superfamily.
PTM databases
PhosphoSite	Q6P499.
Polymorphism databases
DMDM	74737314.
Proteomic databases
PRIDE	Q6P499.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000003912; ENSP00000003912; ENSG00000001461. ENST00000358028; ENSP00000350722; ENSG00000001461. ENST00000374399; ENSP00000363520; ENSG00000001461. ENST00000428131; ENSP00000406509; ENSG00000001461.
GeneID	57185.
KEGG	hsa:57185.
UCSC	uc001bjh.3. human. uc010oek.2. human.
Organism-specific databases
CTD	57185.
GeneCards	GC01P024742.
H-InvDB	HIX0000263.
HGNC	HGNC:25233. NIPAL3.
HPA	HPA039978.
neXtProt	NX_Q6P499.
PharmGKB	PA164723927.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG238068.
HOGENOM	HOG000230865.
HOVERGEN	HBG069791.
InParanoid	Q6P499.
OMA	WWLGLFL.
OrthoDB	EOG4R5030.
Gene expression databases
ArrayExpress	Q6P499.
Bgee	Q6P499.
CleanEx	HS_NPAL3.
Genevestigator	Q6P499.
GermOnline	ENSG00000001461. Homo sapiens.
Family and domain databases
InterPro	IPR008521. Mg_trans_NIPA. [Graphical view]
PANTHER	PTHR12570. PTHR12570. 1 hit.
Pfam	PF05653. Mg_trans_NIPA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	NIPAL3. human.
GenomeRNAi	57185.
NextBio	63240.

Entry information

Entry name

NPAL3_HUMAN

Accession

Primary (citable) accession number: Q6P499
Secondary accession number(s): A2A298, Q6MZT9, Q9BVE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	July 5, 2004
Last modified:	March 6, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.