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Protein

SCY1-like protein 2

Gene

SCYL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of AP2-containing clathrin coated structures at the plasma membrane or of endocytic coated vesicles. According to PubMed:15809293, probable serine/threonine-protein kinase that phosphorylates, in vitro, the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L-lysine. According to PubMed:16914521, has no detectable kinase activity in vitro. May regulate clathrin-dependent trafficking between the TGN and/or the endosomal system.2 Publications

GO - Molecular functioni

  • ATP binding Source: InterPro
  • protein kinase activity Source: InterPro
  • receptor binding Source: BHF-UCL

GO - Biological processi

  • endosome to lysosome transport Source: BHF-UCL
  • negative regulation of canonical Wnt signaling pathway Source: BHF-UCL
  • positive regulation of clathrin-mediated endocytosis Source: BHF-UCL
  • positive regulation of receptor internalization Source: BHF-UCL
  • receptor internalization involved in canonical Wnt signaling pathway Source: BHF-UCL
Complete GO annotation...

Enzyme and pathway databases

SignaLinkiQ6P3W7.

Names & Taxonomyi

Protein namesi
Recommended name:
SCY1-like protein 2
Alternative name(s):
Coated vesicle-associated kinase of 104 kDa
Gene namesi
Name:SCYL2
Synonyms:CVAK104, KIAA1360
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:19286. SCYL2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134887807.

Polymorphism and mutation databases

BioMutaiSCYL2.
DMDMi74762350.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 929929SCY1-like protein 2PRO_0000252446Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei677 – 6771PhosphoserineCombined sources
Modified residuei708 – 7081PhosphothreonineCombined sources

Post-translational modificationi

According to PubMed:15809293, autophosphorylated in presence of poly-L-lysine.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6P3W7.
MaxQBiQ6P3W7.
PaxDbiQ6P3W7.
PeptideAtlasiQ6P3W7.
PRIDEiQ6P3W7.

PTM databases

iPTMnetiQ6P3W7.
PhosphoSiteiQ6P3W7.

Expressioni

Gene expression databases

BgeeiQ6P3W7.
CleanExiHS_SCYL2.
ExpressionAtlasiQ6P3W7. baseline and differential.
GenevisibleiQ6P3W7. HS.

Organism-specific databases

HPAiHPA021050.

Interactioni

Subunit structurei

Interacts with clathrin and AP2B1; the interaction mediates the association with the AP-2 complex.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DVL1O146404EBI-1046810,EBI-723489
FZD5Q134674EBI-1046810,EBI-3913027

GO - Molecular functioni

  • receptor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi120810. 31 interactions.
IntActiQ6P3W7. 17 interactions.
MINTiMINT-1181355.
STRINGi9606.ENSP00000354061.

Structurei

3D structure databases

ProteinModelPortaliQ6P3W7.
SMRiQ6P3W7. Positions 31-344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 327296Protein kinasePROSITE-ProRule annotationAdd
BLAST
Repeati443 – 47937HEATAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni699 – 929231Necessary for interaction with AP2 complex and clathrin, interaction with clathrin is necessary for its targeting to the TGN and endosomal membranesAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili661 – 70141Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily.Curated
Contains 1 HEAT repeat.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2137. Eukaryota.
ENOG410XQ9T. LUCA.
GeneTreeiENSGT00500000044907.
HOVERGENiHBG055627.
InParanoidiQ6P3W7.
KOiK17541.
OMAiWFVIDEI.
PhylomeDBiQ6P3W7.
TreeFamiTF314178.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6P3W7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESMLNKLKS TVTKVTADVT SAVMGNPVTR EFDVGRHIAS GGNGLAWKIF
60 70 80 90 100
NGTKKSTKQE VAVFVFDKKL IDKYQKFEKD QIIDSLKRGV QQLTRLRHPR
110 120 130 140 150
LLTVQHPLEE SRDCLAFCTE PVFASLANVL GNWENLPSPI SPDIKDYKLY
160 170 180 190 200
DVETKYGLLQ VSEGLSFLHS SVKMVHGNIT PENIILNKSG AWKIMGFDFC
210 220 230 240 250
VSSTNPSEQE PKFPCKEWDP NLPSLCLPNP EYLAPEYILS VSCETASDMY
260 270 280 290 300
SLGTVMYAVF NKGKPIFEVN KQDIYKSFSR QLDQLSRLGS SSLTNIPEEV
310 320 330 340 350
REHVKLLLNV TPTVRPDADQ MTKIPFFDDV GAVTLQYFDT LFQRDNLQKS
360 370 380 390 400
QFFKGLPKVL PKLPKRVIVQ RILPCLTSEF VNPDMVPFVL PNVLLIAEEC
410 420 430 440 450
TKEEYVKLIL PELGPVFKQQ EPIQILLIFL QKMDLLLTKT PPDEIKNSVL
460 470 480 490 500
PMVYRALEAP SIQIQELCLN IIPTFANLID YPSMKNALIP RIKNACLQTS
510 520 530 540 550
SLAVRVNSLV CLGKILEYLD KWFVLDDILP FLQQIPSKEP AVLMGILGIY
560 570 580 590 600
KCTFTHKKLG ITKEQLAGKV LPHLIPLSIE NNLNLNQFNS FISVIKEMLN
610 620 630 640 650
RLESEHKTKL EQLHIMQEQQ KSLDIGNQMN VSEEMKVTNI GNQQIDKVFN
660 670 680 690 700
NIGADLLTGS ESENKEDGLQ NKHKRASLTL EEKQKLAKEQ EQAQKLKSQQ
710 720 730 740 750
PLKPQVHTPV ATVKQTKDLT DTLMDNMSSL TSLSVSTPKS SASSTFTSVP
760 770 780 790 800
SMGIGMMFST PTDNTKRNLT NGLNANMGFQ TSGFNMPVNT NQNFYSSPST
810 820 830 840 850
VGVTKMTLGT PPTLPNFNAL SVPPAGAKQT QQRPTDMSAL NNLFGPQKPK
860 870 880 890 900
VSMNQLSQQK PNQWLNQFVP PQGSPTMGSS VMGTQMNVIG QSAFGMQGNP
910 920
FFNPQNFAQP PTTMTNSSSA SNDLKDLFG
Length:929
Mass (Da):103,709
Last modified:July 5, 2004 - v1
Checksum:i980B481BF7044628
GO

Sequence cautioni

The sequence BAA91778.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAB14869.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAB55194.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti139 – 1457PISPDIK → LMSGDIG in BAA92598 (PubMed:10718198).Curated
Sequence conflicti186 – 1861L → S in BAB14869 (PubMed:14702039).Curated
Sequence conflicti424 – 4241Q → QASNM in BAA92598 (PubMed:10718198).Curated
Sequence conflicti439 – 4391K → R in BAB14869 (PubMed:14702039).Curated
Sequence conflicti554 – 5541F → L in AAH12387 (PubMed:15489334).Curated
Sequence conflicti628 – 6281Q → R in BAB14869 (PubMed:14702039).Curated
Sequence conflicti638 – 6381T → I in AAH12387 (PubMed:15489334).Curated
Sequence conflicti747 – 7471T → A in BAA91778 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti357 – 3571P → L.2 Publications
Corresponds to variant rs33968174 [ dbSNP | Ensembl ].
VAR_041368
Natural varianti720 – 7201T → S.1 Publication
VAR_041369
Natural varianti863 – 8631Q → H in a lung adenocarcinoma sample; somatic mutation. 1 Publication
VAR_041370

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001597 mRNA. Translation: BAA91778.1. Different initiation.
AK024274 mRNA. Translation: BAB14869.1. Different initiation.
AK027551 mRNA. Translation: BAB55194.1. Different initiation.
AK292980 mRNA. Translation: BAF85669.1.
CH471054 Genomic DNA. Translation: EAW97633.1.
BC012387 mRNA. Translation: AAH12387.3.
BC063798 mRNA. Translation: AAH63798.1.
AB037781 mRNA. Translation: BAA92598.1.
CCDSiCCDS9076.1.
RefSeqiNP_001304713.1. NM_001317784.1.
NP_060458.3. NM_017988.5.
UniGeneiHs.506481.

Genome annotation databases

EnsembliENST00000360820; ENSP00000354061; ENSG00000136021.
GeneIDi55681.
KEGGihsa:55681.
UCSCiuc001thn.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001597 mRNA. Translation: BAA91778.1. Different initiation.
AK024274 mRNA. Translation: BAB14869.1. Different initiation.
AK027551 mRNA. Translation: BAB55194.1. Different initiation.
AK292980 mRNA. Translation: BAF85669.1.
CH471054 Genomic DNA. Translation: EAW97633.1.
BC012387 mRNA. Translation: AAH12387.3.
BC063798 mRNA. Translation: AAH63798.1.
AB037781 mRNA. Translation: BAA92598.1.
CCDSiCCDS9076.1.
RefSeqiNP_001304713.1. NM_001317784.1.
NP_060458.3. NM_017988.5.
UniGeneiHs.506481.

3D structure databases

ProteinModelPortaliQ6P3W7.
SMRiQ6P3W7. Positions 31-344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120810. 31 interactions.
IntActiQ6P3W7. 17 interactions.
MINTiMINT-1181355.
STRINGi9606.ENSP00000354061.

PTM databases

iPTMnetiQ6P3W7.
PhosphoSiteiQ6P3W7.

Polymorphism and mutation databases

BioMutaiSCYL2.
DMDMi74762350.

Proteomic databases

EPDiQ6P3W7.
MaxQBiQ6P3W7.
PaxDbiQ6P3W7.
PeptideAtlasiQ6P3W7.
PRIDEiQ6P3W7.

Protocols and materials databases

DNASUi55681.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000360820; ENSP00000354061; ENSG00000136021.
GeneIDi55681.
KEGGihsa:55681.
UCSCiuc001thn.4. human.

Organism-specific databases

CTDi55681.
GeneCardsiSCYL2.
HGNCiHGNC:19286. SCYL2.
HPAiHPA021050.
MIMi616365. gene.
neXtProtiNX_Q6P3W7.
PharmGKBiPA134887807.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2137. Eukaryota.
ENOG410XQ9T. LUCA.
GeneTreeiENSGT00500000044907.
HOVERGENiHBG055627.
InParanoidiQ6P3W7.
KOiK17541.
OMAiWFVIDEI.
PhylomeDBiQ6P3W7.
TreeFamiTF314178.

Enzyme and pathway databases

SignaLinkiQ6P3W7.

Miscellaneous databases

ChiTaRSiSCYL2. human.
GeneWikiiSCYL2.
GenomeRNAii55681.
PROiQ6P3W7.
SOURCEiSearch...

Gene expression databases

BgeeiQ6P3W7.
CleanExiHS_SCYL2.
ExpressionAtlasiQ6P3W7. baseline and differential.
GenevisibleiQ6P3W7. HS.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-357.
    Tissue: Teratocarcinoma and Trachea.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Carcinoma and Leiomyosarcoma.
  4. "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
    DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 138-929.
    Tissue: Brain.
  5. "CVAK104 is a novel poly-L-lysine-stimulated kinase that targets the beta2-subunit of AP2."
    Conner S.D., Schmid S.L.
    J. Biol. Chem. 280:21539-21544(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CLATHRIN AND AP2 COMPLEX, AUTOPHOSPHORYLATION, SUBCELLULAR LOCATION.
  6. "Clathrin-dependent association of CVAK104 with endosomes and the trans-Golgi network."
    Duewel M., Ungewickell E.J.
    Mol. Biol. Cell 17:4513-4525(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CLATHRIN AND AP2 COMPLEX, SUBCELLULAR LOCATION.
  7. "Role of the AP2 beta-appendage hub in recruiting partners for clathrin-coated vesicle assembly."
    Schmid E.M., Ford M.G.J., Burtey A., Praefcke G.J.K., Peak-Chew S.-Y., Mills I.G., Benmerah A., McMahon H.T.
    PLoS Biol. 4:E262-E262(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AP2B1.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-677, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Toward a comprehensive characterization of a human cancer cell phosphoproteome."
    Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., Mohammed S.
    J. Proteome Res. 12:260-271(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-677 AND THR-708, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma and Erythroleukemia.
  11. "Patterns of somatic mutation in human cancer genomes."
    Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
    , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
    Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS [LARGE SCALE ANALYSIS] LEU-357; SER-720 AND HIS-863.

Entry informationi

Entry nameiSCYL2_HUMAN
AccessioniPrimary (citable) accession number: Q6P3W7
Secondary accession number(s): A8KAB5
, Q96EF4, Q96ST4, Q9H7V5, Q9NVH3, Q9P2I7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: July 5, 2004
Last modified: July 6, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.