Q6P3P5 (B3GN5_XENTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase
EC=2.4.1.206

Alternative name(s):
Lactotriaosylceramide synthase
Short name=Lc(3)Cer synthase
Short name=Lc3 synthase
UDP-GlcNAc:beta-Gal beta-1,3-N-acetylglucosaminyltransferase 5
Short name=BGnT-5
Short name=Beta-1,3-Gn-T5
Short name=Beta-1,3-N-acetylglucosaminyltransferase 5
Short name=Beta3Gn-T5

Gene names

Name:	b3gnt5
ORF Names:	TEgg092f15.1

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 31 family.
Sequence caution	The sequence CAL49301.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	galactosyltransferase activity Inferred from electronic annotation. Source: InterPro lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase		PRO_0000289217
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 30	18	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	31 – 377	347	Lumenal Potential
Amino acid modifications
	Glycosylation	56	1	N-linked (GlcNAc...) Potential
	Glycosylation	167	1	N-linked (GlcNAc...) Potential
	Glycosylation	275	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6P3P5 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: EA39668B455412AF	FASTA	377	43,330
	10 20 30 40 50 60 MLISARRLRR CQFFQLLTSC FVLSLMALLV QEDNSLINHV KSYSYRYLIN SYDFVNDSLS 70 80 90 100 110 120 VPRDRPDGAP SYRYLINNRD KCQNEDVLLL LFVKTSPENR RRRNAIRKTW GNEDYIRSQY 130 140 150 160 170 180 AANIKVVFAL GIEADPVKSH QTQKDLVIEN KRFNDLIQQD FKDTFHNLTL KLLLQFGWVN 190 200 210 220 230 240 SYCPSAKFIM SADDDIFVHT PNLVSYLKSL PIETQDFWIG RVHRGSPPIR SKTSKYYVPY 250 260 270 280 290 300 EMYPWSSYPD YTAGAAYVVS KDVAAKVYEA SQTLNTSLYI DDVFMGICAN KMGVVPQYHV 310 320 330 340 350 360 YFAGEGKAPY HPCIYNKMIT SHGHLDDLDY LWRQATDPNV KSLSAGVLGG AYCKLVNIML 370 LCKLSYVDTY PCSAAWS

References

[1]	Sanger Xenopus tropicalis EST/cDNA project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Egg.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR848570 mRNA. Translation: CAL49301.1. Different initiation. BC063912 mRNA. Translation: AAH63912.1. BC080164 mRNA. Translation: AAH80164.1.
RefSeq	NP_989240.1. NM_203909.1.
UniGene	Str.42023.
3D structure databases
ProteinModelPortal	Q6P3P5.
ModBase	Search...
Protein-protein interaction databases
STRING	8364.ENSXETP00000055015.
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSXETT00000055015; ENSXETP00000055015; ENSXETG00000025974.
GeneID	394849.
KEGG	xtr:394849.
Organism-specific databases
CTD	84002.
Xenbase	XB-GENE-955708. b3gnt5.
Phylogenomic databases
eggNOG	NOG253164.
GeneTree	ENSGT00670000097716.
HOGENOM	HOG000064519.
HOVERGEN	HBG101684.
InParanoid	Q6P3P5.
KO	K03766.
OMA	SENFERR.
OrthoDB	EOG4PNXH6.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
Bgee	Q6P3P5.
Family and domain databases
InterPro	IPR002659. Glyco_trans_31. [Graphical view]
PANTHER	PTHR11214. PTHR11214. 1 hit.
Pfam	PF01762. Galactosyl_T. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3GN5_XENTR

Accession

Primary (citable) accession number: Q6P3P5
Secondary accession number(s): Q07G20

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program