Reviewed, UniProtKB/Swiss-Prot Q6P3N6 (CDK8_XENTR)

Names and origin

Protein names

Recommended name:
    Cell division protein kinase 8
    EC=2.7.11.22
    EC=2.7.11.23
Alternative name(s):
    Cyclin-dependent kinase 8
    Mediator of RNA polymerase II transcription subunit cdk8
    Mediator complex subunit cdk8

Gene names

Name:

cdk8

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Cofactor	Magnesium By similarity.
Subunit structure	Component of the Mediator complex By similarity. Interacts with ccnc By similarity.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Repressor Serine/threonine-protein kinase Transferase
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Cell division protein kinase 8		PRO_0000312928
Regions
	Domain	21 – 335	315	Protein kinase
	Nucleotide binding	27 – 35	9	ATP By similarity
	Compositional bias	373 – 377	5	Poly-Gln
Sites
	Active site	151	1	Proton acceptor By similarity
	Binding site	52	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6P3N6-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: A4B94F3B25AA207C	FASTA	464	53,350
	10 20 30 40 50 60 MDYDFKVKLT GERERVEDLF EYEGCKVGRG TYGHVYKAKR KDGRDDRDYA LKQIEGTGIS 70 80 90 100 110 120 MSACREIALL RELKHPNVIS LQKVFLSHAD RKVWLLFDFA EHDLWHIIKF HRASKANKKP 130 140 150 160 170 180 VQLPRGMVKS LLYQILDGIH YLHANWVLHR DLKPANILVM GEGPERGRVK IADMGFARLF 190 200 210 220 230 240 NSPLKPLADL DPVVVTFWYR APELLLGARH YTKAIDIWAI GCIFAELLTS EPIFHCRQED 250 260 270 280 290 300 IKTSNPYHHD QLDRIFNVMG FPADKDWEDI KKMPEHSTLI KDFRRNTYTN CSLIKYMEKH 310 320 330 340 350 360 KVKPDSKTFH LLQKLLTMDP IKRISSEQAM QDPYFLEDPL PTSDVFAGCQ IPYPKREFLT 370 380 390 400 410 420 EEEPDDKGDK KNQQQQQGNN HTNGTGHPGN QDNSHAQGPP LKKVRVVPPT TTSGGLIMTS 430 440 450 460 DYQRSNPHAA YQNPGPSTSQ PQSSMGYSST SQQPPQYSHQ THRY

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC063921 mRNA. Translation: AAH63921.1.
RefSeq	NP_989246.1.
UniGene	Str.6945
3D structure databases
HSSP	HSSP built from PDB template 1OIT based on UniProtKB P24941.
ModBase	Search...
Genome annotation databases
Ensembl	ENSXETT00000021559; ENSXETP00000021559; ENSXETG00000009787; Xenopus tropicalis. [Genome view]
GeneID	394856.
KEGG	xtr:394856.
Organism-specific databases
CTD	394856.
Xenbase	XB-FEAT-962266. cdk8.
Phylogenomic databases
eggNOG	veNOG05624.
HOGENOM	HBG755340.
HOVERGEN	Q6P3N6.
InParanoid	Q6P3N6.
Enzyme and pathway databases
BRENDA	2.7.11.22. 279072. 2.7.11.23. 279072.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDK8_XENTR

Accession

Primary (citable) accession number: Q6P3N6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 5, 2004
Last modified:	January 19, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project