Reviewed, UniProtKB/Swiss-Prot Q6P3L6 (DH12A_DANRE)

Names and origin

Protein names

Recommended name:
    Estradiol 17-beta-dehydrogenase 12-A
    EC=1.1.1.62
Alternative name(s):
    17-beta-hydroxysteroid dehydrogenase 12-A
      Short name=17-beta-HSD 12-A
      Short name=zfHSD17B12A
      Short name=zf3.1

Gene names

Name:	hsd17b12a
ORF Names:	zgc:55589

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation By similarity.
Catalytic activity	Estradiol-17-beta + NAD(P)+ = estrone + NAD(P)H.
Pathway	Steroid biosynthesis; estrogen biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Developmental stage	Expressed throughout development. Weakly expressed during early developmental stages from shield to tailbud. Ref.1
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro estradiol 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Estradiol 17-beta-dehydrogenase 12-A		PRO_0000248371
Regions
	Transmembrane	17 – 37	21	Potential
	Transmembrane	188 – 208	21	Potential
	Transmembrane	282 – 302	21	Potential
	Nucleotide binding	56 – 85	30	NADP By similarity
Sites
	Active site	208	1	Proton acceptor By similarity
	Binding site	195	1	Substrate By similarity
Experimental info
	Sequence conflict	187	1	K → E in AAH48053. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q6P3L6-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: BA0892BF0B7A82C8	FASTA	319	35,551
	10 20 30 40 50 60 MESFNVVETL QPAERALFWV GALITASLAL YVVYKTITGF RIWVLGNGDL LSPKLGKWAV 70 80 90 100 110 120 VTGATDGIGK SYAEELARRG FSMMLISRSQ EKLDDVAKSL ESTYKVETKT IAVDFSQIDV 130 140 150 160 170 180 YPKIEKGLAG LEIGILVNNV GISYSYPEFF LHIPDLENFI TTMINVNITS VCQMTRLVLP 190 200 210 220 230 240 RMEARAKGVI LNISSASGMF PVPLLTIYSS TKAFVDFFSR GLQTEYKCKG IIIQSVLPFF 250 260 270 280 290 300 VATKMTKIRK PTLDKPTPER YVAAELNTVG LQDQTNGYFP HAVMGWVTTI LAPIDLVLNL 310 GLRMNKAQRG GYLRRRKLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of 17 beta-hydroxysteroid dehydrogenases in the zebrafish, Danio rerio." Mindnich R., Deluca D., Adamski J. Mol. Cell. Endocrinol. 215:19-30(2004) [PubMed: 15026171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: AB.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY551082 mRNA. Translation: AAS58452.1. BC048053 mRNA. Translation: AAH48053.1. BC063943 mRNA. Translation: AAH63943.1.
IPI	IPI00508221.
RefSeq	NP_957175.1.
UniGene	Dr.29406
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6P3L6.
Genome annotation databases
Ensembl	ENSDART00000005299; ENSDARP00000014003; ENSDARG00000015709; Danio rerio. [Genome view] ENSDART00000109423; ENSDARP00000097900; ENSDARG00000015709; Danio rerio. [Genome view]
GeneID	327417.
KEGG	dre:327417.
Organism-specific databases
CTD	327417.
ZFIN	ZDB-GENE-030131-5628. hsd17b12a.
Phylogenomic databases
HOVERGEN	Q6P3L6.
OMA	QTITPLM.
Enzyme and pathway databases
BRENDA	1.1.1.62. 96826.
Gene expression databases
ArrayExpress	Q6P3L6.
Bgee	Q6P3L6.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DH12A_DANRE

Accession

Primary (citable) accession number: Q6P3L6
Secondary accession number(s): Q7ZUN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project