Q6P371 (HOT_XENTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacid-oxoacid transhydrogenase, mitochondrial
Short name=HOT
EC=1.1.99.24

Alternative name(s):
Alcohol dehydrogenase iron-containing protein 1
Short name=ADHFe1

Gene names

Name:

adhfe1

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG By similarity.
Catalytic activity	(S)-3-hydroxybutanoate + 2-oxoglutarate = acetoacetate + (R)-2-hydroxyglutarate. 4-hydroxybutanoate + 2-oxoglutarate = succinic semialdehyde + (R)-2-hydroxyglutarate.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	molecular hydrogen transport Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	hydroxyacid-oxoacid transhydrogenase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 463		Hydroxyacid-oxoacid transhydrogenase, mitochondrial		PRO_0000323001

Sequences

	Sequence		Length	Mass (Da)
	Q6P371 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: F6CD5F2B90C513C1	FASTA	463	50,408
	10 20 30 40 50 60 MAARRDQVIH LLRQLQRASC KCPAHSHTYS QAPVTGRKTE YAFEMAVSNI RYGENVTQEI 70 80 90 100 110 120 GMDLQNLGAR NVCVMTDRNL VELSPVKAVL NSLVKNNVSF KLYDRVRVEP TDKSFMDAIE 130 140 150 160 170 180 FAKKGQFDAY VGVGGGSVID TCKAANLYSS SPGADFLDYV NPPIGKGKAV TVPLKPLIAV 190 200 210 220 230 240 PTTSGTGSET TGIAIFDYEE LKAKTGIASR AIKPTLGLID PVHTLSMPER VVANSGFDVL 250 260 270 280 290 300 CHSLESYTAL PYNMRSPCPT NPINRPAYQG SNPISDVWAK HALRIVAKYL KRAVRNPDDR 310 320 330 340 350 360 EARFAMHLAS SFAGVGFGNA GVHLCHGMSY PIAGHVKTYR AKDYEVDHPL VPHGLSVVLT 370 380 390 400 410 420 SPAVFSFTAL MCPERHLEAA EILGADIRTA KIKEAGLILA DTLRKFLYDL NVDDGLAAVG 430 440 450 460 YTTEDIPALV KGTLPQERVT KLSPRAHSEE ELAGLFEASM KLY

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC064162 mRNA. Translation: AAH64162.1.
RefSeq	NP_989277.1. NM_203946.1.
UniGene	Str.5492.
3D structure databases
ProteinModelPortal	Q6P371.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6P371.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	394891.
KEGG	xtr:394891.
Organism-specific databases
CTD	137872.
Xenbase	XB-GENE-966429. adhfe1.
Phylogenomic databases
eggNOG	veNOG09006.
GeneTree	ENSGT00390000003849.
HOGENOM	HBG742415.
HOVERGEN	HBG057032.
InParanoid	Q6P371.
OrthoDB	EOG4HMJ98.
Gene expression databases
Bgee	Q6P371.
Family and domain databases
InterPro	IPR001670. ADH_Fe. [Graphical view]
KO	K11173.
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. False negative. PS00060. ADH_IRON_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOT_XENTR

Accession

Primary (citable) accession number: Q6P371

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program