Q6P2M8 (KCC1B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calcium/calmodulin-dependent protein kinase type 1B
EC=2.7.11.17

Alternative name(s):
CaM kinase I beta
Short name=CaM kinase IB
Short name=CaM-KI beta
Short name=CaMKI-beta
Pregnancy up-regulated non-ubiquitously-expressed CaM kinase

Gene names

Name:

PNCK

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates CREB1 and SYN1/synapsin I. Phosphorylates and activates CAMK1 By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Activated by Ca2+/calmodulin By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Post-translational modification	Phosphorylated by CAMKK1 Probable.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Calcium Calmodulin-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6P2M8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Gene prediction based on similarity to mouse ortholog.

Isoform 2 (identifier: Q6P2M8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     179-179: V → AEPVTLSGMAACVPVRASVCLWPP

Isoform 3 (identifier: Q6P2M8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-102: Missing.

Isoform 4 (identifier: Q6P2M8-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MEAAFGQVAG...RIPAIALQDM

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Calcium/calmodulin-dependent protein kinase type 1B		PRO_0000086079
Regions
	Domain	15 – 270	256	Protein kinase
	Nucleotide binding	21 – 29	9	ATP By similarity
	Region	290 – 311	22	Calmodulin-binding By similarity
Sites
	Active site	136	1	Proton acceptor By similarity
	Binding site	44	1	ATP By similarity
Natural variations
	Alternative sequence	1 – 102	102	Missing in isoform 3.		VSP_012634
	Alternative sequence	1	1	M → MEAAFGQVAGSACPRRGGEG RDWKAESLADLWPKSSPGDS HRWCKGPGAGPAGPQLREAA RASSGLGGGGRHPSRIPAIA LQDM in isoform 4.		VSP_039091
	Alternative sequence	179	1	V → AEPVTLSGMAACVPVRASVC LWPP in isoform 2.		VSP_012635
	Natural variant	262	1	Q → H. Ref.6		VAR_040598
Experimental info
	Sequence conflict	127	1	L → P in BAG58930. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 2005. Version 2. Checksum: E954AEE89DE608C9	FASTA	343	38,500
	10 20 30 40 50 60 MLLLKKHTED ISSVYEIRER LGSGAFSEVV LAQERGSAHL VALKCIPKKA LRGKEALVEN 70 80 90 100 110 120 EIAVLRRISH PNIVALEDVH ESPSHLYLAM ELVTGGELFD RIMERGSYTE KDASHLVGQV 130 140 150 160 170 180 LGAVSYLHSL GIVHRDLKPE NLLYATPFED SKIMVSDFGL SKIQAGNMLG TACGTPGYVA 190 200 210 220 230 240 PELLEQKPYG KAVDVWALGV ISYILLCGYP PFYDESDPEL FSQILRASYE FDSPFWDDIS 250 260 270 280 290 300 ESAKDFIRHL LERDPQKRFT CQQALRHLWI SGDTAFDRDI LGSVSEQIRK NFARTHWKRA 310 320 330 340 FNATSFLRHI RKLGQIPEGE GASEQGMARH SHSGLRAGQP PKW
	Isoform 2 [UniParc]. Checksum: F9F61FA92579FDE9	FASTA	366	40,838
	10 20 30 40 50 60 MLLLKKHTED ISSVYEIRER LGSGAFSEVV LAQERGSAHL VALKCIPKKA LRGKEALVEN 70 80 90 100 110 120 EIAVLRRISH PNIVALEDVH ESPSHLYLAM ELVTGGELFD RIMERGSYTE KDASHLVGQV 130 140 150 160 170 180 LGAVSYLHSL GIVHRDLKPE NLLYATPFED SKIMVSDFGL SKIQAGNMLG TACGTPGYAE 190 200 210 220 230 240 PVTLSGMAAC VPVRASVCLW PPAPELLEQK PYGKAVDVWA LGVISYILLC GYPPFYDESD 250 260 270 280 290 300 PELFSQILRA SYEFDSPFWD DISESAKDFI RHLLERDPQK RFTCQQALRH LWISGDTAFD 310 320 330 340 350 360 RDILGSVSEQ IRKNFARTHW KRAFNATSFL RHIRKLGQIP EGEGASEQGM ARHSHSGLRA GQPPKW
	Isoform 3 [UniParc]. Checksum: 6AA7E6AFE0CEB409	FASTA	241	27,161
	10 20 30 40 50 60 MERGSYTEKD ASHLVGQVLG AVSYLHSLGI VHRDLKPENL LYATPFEDSK IMVSDFGLSK 70 80 90 100 110 120 IQAGNMLGTA CGTPGYVAPE LLEQKPYGKA VDVWALGVIS YILLCGYPPF YDESDPELFS 130 140 150 160 170 180 QILRASYEFD SPFWDDISES AKDFIRHLLE RDPQKRFTCQ QALRHLWISG DTAFDRDILG 190 200 210 220 230 240 SVSEQIRKNF ARTHWKRAFN ATSFLRHIRK LGQIPEGEGA SEQGMARHSH SGLRAGQPPK W
	Isoform 4 [UniParc]. Checksum: A2958437908C9C67	FASTA	426	46,957
	10 20 30 40 50 60 MEAAFGQVAG SACPRRGGEG RDWKAESLAD LWPKSSPGDS HRWCKGPGAG PAGPQLREAA 70 80 90 100 110 120 RASSGLGGGG RHPSRIPAIA LQDMLLLKKH TEDISSVYEI RERLGSGAFS EVVLAQERGS 130 140 150 160 170 180 AHLVALKCIP KKALRGKEAL VENEIAVLRR ISHPNIVALE DVHESPSHLY LAMELVTGGE 190 200 210 220 230 240 LFDRIMERGS YTEKDASHLV GQVLGAVSYL HSLGIVHRDL KPENLLYATP FEDSKIMVSD 250 260 270 280 290 300 FGLSKIQAGN MLGTACGTPG YVAPELLEQK PYGKAVDVWA LGVISYILLC GYPPFYDESD 310 320 330 340 350 360 PELFSQILRA SYEFDSPFWD DISESAKDFI RHLLERDPQK RFTCQQALRH LWISGDTAFD 370 380 390 400 410 420 RDILGSVSEQ IRKNFARTHW KRAFNATSFL RHIRKLGQIP EGEGASEQGM ARHSHSGLRA GQPPKW

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Thalamus.
[2]	"Full-length cDNA libraries and normalization." Li W.B., Gruber C., Jessee J., Polayes D. Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Placenta.
[3]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed: 15772651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain.
[6]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] HIS-262.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK296203 mRNA. Translation: BAG58930.1. CR611192 mRNA. No translation available. U52111 Genomic DNA. No translation available. CH471172 Genomic DNA. Translation: EAW72838.1. CH471172 Genomic DNA. Translation: EAW72839.1. CH471172 Genomic DNA. Translation: EAW72841.1. CH471172 Genomic DNA. Translation: EAW72842.1. CH471172 Genomic DNA. Translation: EAW72843.1. CH471172 Genomic DNA. Translation: EAW72845.1. BC064422 mRNA. Translation: AAH64422.1.
IPI	IPI00413306. IPI00550276. IPI00639968. IPI00913860.
RefSeq	NP_001034671.3. NM_001039582.3.
UniGene	Hs.436667.
3D structure databases
ProteinModelPortal	Q6P2M8.
SMR	Q6P2M8. Positions 5-304.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6P2M8.
PTM databases
PhosphoSite	Q6P2M8.
Polymorphism databases
DMDM	67466915.
Proteomic databases
PRIDE	Q6P2M8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000340888; ENSP00000340586; ENSG00000130822. ENST00000370142; ENSP00000359161; ENSG00000130822. ENST00000370145; ENSP00000359164; ENSG00000130822. ENST00000370150; ENSP00000359169; ENSG00000130822. ENST00000393831; ENSP00000377417; ENSG00000130822. ENST00000447676; ENSP00000405950; ENSG00000130822.
GeneID	139728.
KEGG	hsa:139728.
UCSC	uc004fhz.2. human. uc004fia.1. human.
Organism-specific databases
CTD	139728.
GeneCards	GC0XM152935.
H-InvDB	HIX0017133.
HGNC	HGNC:13415. PNCK.
HPA	HPA007458.
MIM	300680. gene.
neXtProt	NX_Q6P2M8.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG14938.
GeneTree	ENSGT00600000084207.
HOVERGEN	HBG108055.
OMA	ALDKNIL.
OrthoDB	EOG4T4CVW.
PhylomeDB	Q6P2M8.
Gene expression databases
ArrayExpress	Q6P2M8.
Bgee	Q6P2M8.
CleanEx	HS_PNCK.
Genevestigator	Q6P2M8.
GermOnline	ENSG00000130822. Homo sapiens.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K08795.
PANTHER	PTHR24347. PTHR24347. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	84022.
SOURCE	Search...

Entry information

Entry name

KCC1B_HUMAN

Accession

Primary (citable) accession number: Q6P2M8
Secondary accession number(s): B4DJR8 , B7WPG0, D3DWU7, Q8N4R0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	February 1, 2005
Last modified:	January 25, 2012
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.