Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Histone-lysine N-methyltransferase NSD3

Gene

Whsc1l1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Histone methyltransferase. Preferentially methylates 'Lys-4' and 'Lys-27' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri701 – 74848PHD-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri749 – 80557PHD-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri862 – 95594PHD-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1323 – 137048PHD-type 4; atypicalPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Methyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-3214841. PKMTs methylate histone lysines.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase NSD3 (EC:2.1.1.43)
Alternative name(s):
Nuclear SET domain-containing protein 3
Wolf-Hirschhorn syndrome candidate 1-like protein 1 homolog
Short name:
WHSC1-like protein 1
Gene namesi
Name:Whsc1l1
Synonyms:Nsd3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2142581. Whsc1l1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14391439Histone-lysine N-methyltransferase NSD3PRO_0000259522Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei150 – 1501PhosphoserineBy similarity
Cross-linki218 – 218Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki245 – 245Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei457 – 4571PhosphoserineBy similarity
Cross-linki532 – 532Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei585 – 5851PhosphoserineBy similarity
Modified residuei587 – 5871PhosphoserineBy similarity
Modified residuei590 – 5901PhosphoserineBy similarity
Modified residuei655 – 6551PhosphoserineBy similarity
Modified residuei790 – 7901N6-acetyllysineCombined sources
Cross-linki1154 – 1154Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ6P2L6.
PaxDbiQ6P2L6.
PeptideAtlasiQ6P2L6.
PRIDEiQ6P2L6.

PTM databases

iPTMnetiQ6P2L6.
PhosphoSiteiQ6P2L6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000054823.
ExpressionAtlasiQ6P2L6. baseline and differential.
GenevisibleiQ6P2L6. MM.

Interactioni

Subunit structurei

Interacts with BRD4.By similarity

Protein-protein interaction databases

BioGridi231497. 1 interaction.
IntActiQ6P2L6. 2 interactions.
MINTiMINT-4110979.
STRINGi10090.ENSMUSP00000081040.

Structurei

3D structure databases

ProteinModelPortaliQ6P2L6.
SMRiQ6P2L6. Positions 957-1067, 1073-1287, 1321-1415.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini270 – 33364PWWP 1PROSITE-ProRule annotationAdd
BLAST
Domaini960 – 102566PWWP 2PROSITE-ProRule annotationAdd
BLAST
Domaini1096 – 114651AWSPROSITE-ProRule annotationAdd
BLAST
Domaini1148 – 1265118SETPROSITE-ProRule annotationAdd
BLAST
Domaini1272 – 128817Post-SETPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1036 – 106530Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily.PROSITE-ProRule annotation
Contains 1 AWS domain.PROSITE-ProRule annotation
Contains 4 PHD-type zinc fingers.PROSITE-ProRule annotation
Contains 1 post-SET domain.PROSITE-ProRule annotation
Contains 2 PWWP domains.PROSITE-ProRule annotation
Contains 1 SET domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri701 – 74848PHD-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri749 – 80557PHD-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri862 – 95594PHD-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1323 – 137048PHD-type 4; atypicalPROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1081. Eukaryota.
COG2940. LUCA.
GeneTreeiENSGT00780000121845.
HOGENOMiHOG000230893.
HOVERGENiHBG079979.
InParanoidiQ6P2L6.
KOiK11425.

Family and domain databases

Gene3Di3.30.40.10. 3 hits.
InterProiIPR006560. AWS_dom.
IPR003616. Post-SET_dom.
IPR000313. PWWP_dom.
IPR001214. SET_dom.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00855. PWWP. 2 hits.
PF00856. SET. 1 hit.
[Graphical view]
SMARTiSM00570. AWS. 1 hit.
SM00249. PHD. 5 hits.
SM00508. PostSET. 1 hit.
SM00293. PWWP. 2 hits.
SM00317. SET. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 3 hits.
PROSITEiPS51215. AWS. 1 hit.
PS50868. POST_SET. 1 hit.
PS50812. PWWP. 2 hits.
PS50280. SET. 1 hit.
PS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 2 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6P2L6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDFSFSFMQG IMGNTIQQPP QLIDSANIRQ EDAFDNHSDI VEDGGPTPFE
60 70 80 90 100
ATLQQGFQYP PTTEDLPPLT NGYPPSISLY ETQTKYPPYN QYPNGSANGF
110 120 130 140 150
GAVRNFSPTD YYHSEIPNTR PHEILEKPSP PQPPPPPSVP QTVIPKKTGS
160 170 180 190 200
PEIKLKITKT IQNGRELFES SLCGDLLNEV QASEHTKSKH ESRKEKRKKS
210 220 230 240 250
NRHESSRSEE RRSHKIPKLE PEGQNRPNER VDTAPEKPRE EPVLKEAIPV
260 270 280 290 300
QPILSSVPTT ETSTGVKFQV GDLVWSKVGT YPWWPCMVSS DPQLEVHSKI
310 320 330 340 350
NTRGAREYHV QFFSNQPERA WVHEKRVREY KGHEQYEELL AEAAKQASNH
360 370 380 390 400
SEKQKIRKPR PQRERAQWDI GIAHAEKALK MTREERVEQY TFIYIDKQPE
410 420 430 440 450
EASSQAKKNV TSKTEVKKPR RPRSVLNSQP EQTNAGEVAS SQSSTDLRRQ
460 470 480 490 500
SQRRHTSLEE EEPPPVKIAW KTAAARKSLP ASITMHKGSL DLQKCNMSPV
510 520 530 540 550
VKIEQVFALQ NATGDGKFID QFVYSTKGIG NKTEISVRGQ DRLIISSPSQ
560 570 580 590 600
RSEKPAQSAS SPEATSGSAG PVEKKQQRRS IRTRSESEKS AEVVPKKKIK
610 620 630 640 650
KEQVETAPQA SLKTGLQKGA SEISDSCKPL KKRSRASTDV ETASCTYRDT
660 670 680 690 700
SDSDSRGLSD GQVGFGKQVD SPSATADADA SDAQSVDSSL SRRGVGTSKK
710 720 730 740 750
DTVCQVCEKA GDCLVACEGE CCRHFHVECL GLTAVPEGHF TCEECETGQH
760 770 780 790 800
PCFSCKVSGK DVKRCSVSVC GKFYHEACVR KFPTAIFESK GFRCPQHCCS
810 820 830 840 850
SCSMEKDIHK ASKGRMMRCL RCPVAYHVGD ACVAAGSVSV SSHILICSNH
860 870 880 890 900
SKRSSQSAAI NVGFCFVCAR GLIVQDHSDP MFSSYAYKSH YLLSESNRAE
910 920 930 940 950
LMKLPMIPSS SASKKRCEKG GRLLCCESCP ASFHPECLSI DMPEGCWNCN
960 970 980 990 1000
DCKAGKKLHY KQIVWVKLGN YRQVLWWPAE ICSPRSVPLN IQGLKHDLGD
1010 1020 1030 1040 1050
FPVFFFGSHD YYWVHQGRVF PYVEGDKHFA EGQTSINKTF KKALEEAAKR
1060 1070 1080 1090 1100
FQELKAQRES KEALEMERTS RKPPPYKHIK ANKVIGKVQV QVADLSEIPR
1110 1120 1130 1140 1150
CNCKPGDENP CGLESQCLNR MSQYECHPQV CPAGDRCQNQ CFTKRLYPDA
1160 1170 1180 1190 1200
EVIKTERRGW GLRTKRSIKK GEFVNEYVGE LIDEEECRLR IKRAHENSVT
1210 1220 1230 1240 1250
NFYMLTVTKD RIIDAGPKGN YSRFMNHSCN PNCETQKWTV NGDVRVGLFA
1260 1270 1280 1290 1300
LCDIPAGMEL TFNYNLDCLG NGRTVCHCGA DNCSGFLGVR PKSACTSAVD
1310 1320 1330 1340 1350
EKTKNAKLKK RRKVKAEAKP IHEDYCFQCG DGGELVMCDK KDCPKAYHLL
1360 1370 1380 1390 1400
CLNLTQPPHG KWECPWHRCD ECGSVAVSFC EFCPHSFCKA HGKGALVPSA
1410 1420 1430
LEGRLCCSSH DPASPVSPEY WSKIRCKWES QDSGEEVKE
Length:1,439
Mass (Da):161,002
Last modified:October 31, 2006 - v2
Checksum:i917C7ADCD1248525
GO
Isoform 2 (identifier: Q6P2L6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     135-135: P → PLPPPPPPPPP
     620-645: ASEISDSCKPLKKRSRASTDVETASC → SADRGAQGSVRFSDSSVSAAKEETVD
     646-1439: Missing.

Show »
Length:655
Mass (Da):73,380
Checksum:i358CBD840A5A47C6
GO
Isoform 3 (identifier: Q6P2L6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-941: Missing.
     973-975: Missing.
     1199-1209: Missing.

Note: No experimental confirmation available.
Show »
Length:484
Mass (Da):54,977
Checksum:i562572C7B3100FD4
GO
Isoform 4 (identifier: Q6P2L6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     135-135: P → PLPPPPPPPPP
     603-611: QVETAPQAS → QVGFLHVES
     612-1439: Missing.

Note: No experimental confirmation available.
Show »
Length:621
Mass (Da):70,000
Checksum:i5553A83F48BF9B16
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti122 – 1221H → R in BAE33834 (PubMed:16141072).Curated
Sequence conflicti950 – 9501N → K in BAE21322 (PubMed:16141072).Curated
Sequence conflicti1149 – 11491D → N in BAE21322 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 941941Missing in isoform 3. 1 PublicationVSP_021431Add
BLAST
Alternative sequencei135 – 1351P → PLPPPPPPPPP in isoform 2 and isoform 4. 2 PublicationsVSP_021432
Alternative sequencei603 – 6119QVETAPQAS → QVGFLHVES in isoform 4. 1 PublicationVSP_021433
Alternative sequencei612 – 1439828Missing in isoform 4. 1 PublicationVSP_021434Add
BLAST
Alternative sequencei620 – 64526ASEIS…ETASC → SADRGAQGSVRFSDSSVSAA KEETVD in isoform 2. 2 PublicationsVSP_021435Add
BLAST
Alternative sequencei646 – 1439794Missing in isoform 2. 2 PublicationsVSP_021436Add
BLAST
Alternative sequencei973 – 9753Missing in isoform 3. 1 PublicationVSP_021437
Alternative sequencei1199 – 120911Missing in isoform 3. 1 PublicationVSP_021438Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK079952 mRNA. Translation: BAC37792.1.
AK132725 mRNA. Translation: BAE21322.1.
AK156746 mRNA. Translation: BAE33834.1.
AK170040 mRNA. Translation: BAE41526.1.
AC156990 Genomic DNA. No translation available.
AC162367 Genomic DNA. No translation available.
BC064447 mRNA. Translation: AAH64447.1.
CCDSiCCDS40305.1. [Q6P2L6-2]
RefSeqiNP_001001735.1. NM_001001735.2. [Q6P2L6-2]
NP_001295410.1. NM_001308481.1. [Q6P2L6-2]
NP_001295411.1. NM_001308482.1. [Q6P2L6-4]
UniGeneiMm.217337.

Genome annotation databases

EnsembliENSMUST00000146919; ENSMUSP00000115470; ENSMUSG00000054823. [Q6P2L6-2]
ENSMUST00000155861; ENSMUSP00000117596; ENSMUSG00000054823. [Q6P2L6-2]
GeneIDi234135.
KEGGimmu:234135.
UCSCiuc009lgk.1. mouse. [Q6P2L6-4]
uc009lgm.1. mouse. [Q6P2L6-2]
uc009lgp.1. mouse. [Q6P2L6-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK079952 mRNA. Translation: BAC37792.1.
AK132725 mRNA. Translation: BAE21322.1.
AK156746 mRNA. Translation: BAE33834.1.
AK170040 mRNA. Translation: BAE41526.1.
AC156990 Genomic DNA. No translation available.
AC162367 Genomic DNA. No translation available.
BC064447 mRNA. Translation: AAH64447.1.
CCDSiCCDS40305.1. [Q6P2L6-2]
RefSeqiNP_001001735.1. NM_001001735.2. [Q6P2L6-2]
NP_001295410.1. NM_001308481.1. [Q6P2L6-2]
NP_001295411.1. NM_001308482.1. [Q6P2L6-4]
UniGeneiMm.217337.

3D structure databases

ProteinModelPortaliQ6P2L6.
SMRiQ6P2L6. Positions 957-1067, 1073-1287, 1321-1415.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231497. 1 interaction.
IntActiQ6P2L6. 2 interactions.
MINTiMINT-4110979.
STRINGi10090.ENSMUSP00000081040.

PTM databases

iPTMnetiQ6P2L6.
PhosphoSiteiQ6P2L6.

Proteomic databases

MaxQBiQ6P2L6.
PaxDbiQ6P2L6.
PeptideAtlasiQ6P2L6.
PRIDEiQ6P2L6.

Protocols and materials databases

DNASUi234135.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000146919; ENSMUSP00000115470; ENSMUSG00000054823. [Q6P2L6-2]
ENSMUST00000155861; ENSMUSP00000117596; ENSMUSG00000054823. [Q6P2L6-2]
GeneIDi234135.
KEGGimmu:234135.
UCSCiuc009lgk.1. mouse. [Q6P2L6-4]
uc009lgm.1. mouse. [Q6P2L6-2]
uc009lgp.1. mouse. [Q6P2L6-3]

Organism-specific databases

CTDi54904.
MGIiMGI:2142581. Whsc1l1.

Phylogenomic databases

eggNOGiKOG1081. Eukaryota.
COG2940. LUCA.
GeneTreeiENSGT00780000121845.
HOGENOMiHOG000230893.
HOVERGENiHBG079979.
InParanoidiQ6P2L6.
KOiK11425.

Enzyme and pathway databases

ReactomeiR-MMU-3214841. PKMTs methylate histone lysines.

Miscellaneous databases

ChiTaRSiWhsc1l1. mouse.
PROiQ6P2L6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000054823.
ExpressionAtlasiQ6P2L6. baseline and differential.
GenevisibleiQ6P2L6. MM.

Family and domain databases

Gene3Di3.30.40.10. 3 hits.
InterProiIPR006560. AWS_dom.
IPR003616. Post-SET_dom.
IPR000313. PWWP_dom.
IPR001214. SET_dom.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00855. PWWP. 2 hits.
PF00856. SET. 1 hit.
[Graphical view]
SMARTiSM00570. AWS. 1 hit.
SM00249. PHD. 5 hits.
SM00508. PostSET. 1 hit.
SM00293. PWWP. 2 hits.
SM00317. SET. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 3 hits.
PROSITEiPS51215. AWS. 1 hit.
PS50868. POST_SET. 1 hit.
PS50812. PWWP. 2 hits.
PS50280. SET. 1 hit.
PS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNSD3_MOUSE
AccessioniPrimary (citable) accession number: Q6P2L6
Secondary accession number(s): Q3TDS4
, Q3U0L8, Q3V131, Q8BJT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 31, 2006
Last modified: September 7, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.