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Q6P2L6 (NSD3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Histone-lysine N-methyltransferase NSD3

EC=2.1.1.43
Alternative name(s):
Nuclear SET domain-containing protein 3
Wolf-Hirschhorn syndrome candidate 1-like protein 1 homolog
Short name=WHSC1-like protein 1
Gene names
Name:Whsc1l1
Synonyms:Nsd3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1439 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Histone methyltransferase. Preferentially methylates 'Lys-4' and 'Lys-27' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression By similarity.

Catalytic activity

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].

Subunit structure

Interacts with BRD4 By similarity.

Subcellular location

Nucleus By similarity. Chromosome By similarity.

Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily.

Contains 1 AWS domain.

Contains 4 PHD-type zinc fingers.

Contains 1 post-SET domain.

Contains 2 PWWP domains.

Contains 1 SET domain.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6P2L6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6P2L6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     135-135: P → PLPPPPPPPPP
     620-645: ASEISDSCKPLKKRSRASTDVETASC → SADRGAQGSVRFSDSSVSAAKEETVD
     646-1439: Missing.
Isoform 3 (identifier: Q6P2L6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-941: Missing.
     973-975: Missing.
     1199-1209: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q6P2L6-4)

The sequence of this isoform differs from the canonical sequence as follows:
     135-135: P → PLPPPPPPPPP
     603-611: QVETAPQAS → QVGFLHVES
     612-1439: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14391439Histone-lysine N-methyltransferase NSD3
PRO_0000259522

Regions

Domain270 – 33364PWWP 1
Domain960 – 102566PWWP 2
Domain1096 – 114651AWS
Domain1148 – 1265118SET
Domain1272 – 128817Post-SET
Zinc finger701 – 74848PHD-type 1
Zinc finger749 – 80557PHD-type 2
Zinc finger862 – 95594PHD-type 3
Zinc finger1323 – 137048PHD-type 4; atypical
Coiled coil1036 – 106530 Potential

Amino acid modifications

Modified residue1501Phosphoserine By similarity
Modified residue7901N6-acetyllysine Ref.7

Natural variations

Alternative sequence1 – 941941Missing in isoform 3.
VSP_021431
Alternative sequence1351P → PLPPPPPPPPP in isoform 2 and isoform 4.
VSP_021432
Alternative sequence603 – 6119QVETAPQAS → QVGFLHVES in isoform 4.
VSP_021433
Alternative sequence612 – 1439828Missing in isoform 4.
VSP_021434
Alternative sequence620 – 64526ASEIS…ETASC → SADRGAQGSVRFSDSSVSAA KEETVD in isoform 2.
VSP_021435
Alternative sequence646 – 1439794Missing in isoform 2.
VSP_021436
Alternative sequence973 – 9753Missing in isoform 3.
VSP_021437
Alternative sequence1199 – 120911Missing in isoform 3.
VSP_021438

Experimental info

Sequence conflict1221H → R in BAE33834. Ref.1
Sequence conflict9501N → K in BAE21322. Ref.1
Sequence conflict11491D → N in BAE21322. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 31, 2006. Version 2.
Checksum: 917C7ADCD1248525

FASTA1,439161,002
        10         20         30         40         50         60 
MDFSFSFMQG IMGNTIQQPP QLIDSANIRQ EDAFDNHSDI VEDGGPTPFE ATLQQGFQYP 

        70         80         90        100        110        120 
PTTEDLPPLT NGYPPSISLY ETQTKYPPYN QYPNGSANGF GAVRNFSPTD YYHSEIPNTR 

       130        140        150        160        170        180 
PHEILEKPSP PQPPPPPSVP QTVIPKKTGS PEIKLKITKT IQNGRELFES SLCGDLLNEV 

       190        200        210        220        230        240 
QASEHTKSKH ESRKEKRKKS NRHESSRSEE RRSHKIPKLE PEGQNRPNER VDTAPEKPRE 

       250        260        270        280        290        300 
EPVLKEAIPV QPILSSVPTT ETSTGVKFQV GDLVWSKVGT YPWWPCMVSS DPQLEVHSKI 

       310        320        330        340        350        360 
NTRGAREYHV QFFSNQPERA WVHEKRVREY KGHEQYEELL AEAAKQASNH SEKQKIRKPR 

       370        380        390        400        410        420 
PQRERAQWDI GIAHAEKALK MTREERVEQY TFIYIDKQPE EASSQAKKNV TSKTEVKKPR 

       430        440        450        460        470        480 
RPRSVLNSQP EQTNAGEVAS SQSSTDLRRQ SQRRHTSLEE EEPPPVKIAW KTAAARKSLP 

       490        500        510        520        530        540 
ASITMHKGSL DLQKCNMSPV VKIEQVFALQ NATGDGKFID QFVYSTKGIG NKTEISVRGQ 

       550        560        570        580        590        600 
DRLIISSPSQ RSEKPAQSAS SPEATSGSAG PVEKKQQRRS IRTRSESEKS AEVVPKKKIK 

       610        620        630        640        650        660 
KEQVETAPQA SLKTGLQKGA SEISDSCKPL KKRSRASTDV ETASCTYRDT SDSDSRGLSD 

       670        680        690        700        710        720 
GQVGFGKQVD SPSATADADA SDAQSVDSSL SRRGVGTSKK DTVCQVCEKA GDCLVACEGE 

       730        740        750        760        770        780 
CCRHFHVECL GLTAVPEGHF TCEECETGQH PCFSCKVSGK DVKRCSVSVC GKFYHEACVR 

       790        800        810        820        830        840 
KFPTAIFESK GFRCPQHCCS SCSMEKDIHK ASKGRMMRCL RCPVAYHVGD ACVAAGSVSV 

       850        860        870        880        890        900 
SSHILICSNH SKRSSQSAAI NVGFCFVCAR GLIVQDHSDP MFSSYAYKSH YLLSESNRAE 

       910        920        930        940        950        960 
LMKLPMIPSS SASKKRCEKG GRLLCCESCP ASFHPECLSI DMPEGCWNCN DCKAGKKLHY 

       970        980        990       1000       1010       1020 
KQIVWVKLGN YRQVLWWPAE ICSPRSVPLN IQGLKHDLGD FPVFFFGSHD YYWVHQGRVF 

      1030       1040       1050       1060       1070       1080 
PYVEGDKHFA EGQTSINKTF KKALEEAAKR FQELKAQRES KEALEMERTS RKPPPYKHIK 

      1090       1100       1110       1120       1130       1140 
ANKVIGKVQV QVADLSEIPR CNCKPGDENP CGLESQCLNR MSQYECHPQV CPAGDRCQNQ 

      1150       1160       1170       1180       1190       1200 
CFTKRLYPDA EVIKTERRGW GLRTKRSIKK GEFVNEYVGE LIDEEECRLR IKRAHENSVT 

      1210       1220       1230       1240       1250       1260 
NFYMLTVTKD RIIDAGPKGN YSRFMNHSCN PNCETQKWTV NGDVRVGLFA LCDIPAGMEL 

      1270       1280       1290       1300       1310       1320 
TFNYNLDCLG NGRTVCHCGA DNCSGFLGVR PKSACTSAVD EKTKNAKLKK RRKVKAEAKP 

      1330       1340       1350       1360       1370       1380 
IHEDYCFQCG DGGELVMCDK KDCPKAYHLL CLNLTQPPHG KWECPWHRCD ECGSVAVSFC 

      1390       1400       1410       1420       1430 
EFCPHSFCKA HGKGALVPSA LEGRLCCSSH DPASPVSPEY WSKIRCKWES QDSGEEVKE 

« Hide

Isoform 2 [UniParc].

Checksum: 358CBD840A5A47C6
Show »

FASTA65573,380
Isoform 3 [UniParc].

Checksum: 562572C7B3100FD4
Show »

FASTA48454,977
Isoform 4 [UniParc].

Checksum: 5553A83F48BF9B16
Show »

FASTA62170,000

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 4).
Strain: C57BL/6J and NOD.
Tissue: Aorta, Spleen, Testis and Vein.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6.
Tissue: Brain.
[4]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 899-914, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[6]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-790, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK079952 mRNA. Translation: BAC37792.1.
AK132725 mRNA. Translation: BAE21322.1.
AK156746 mRNA. Translation: BAE33834.1.
AK170040 mRNA. Translation: BAE41526.1.
AC156990 Genomic DNA. No translation available.
AC162367 Genomic DNA. No translation available.
BC064447 mRNA. Translation: AAH64447.1.
CCDSCCDS40305.1. [Q6P2L6-2]
RefSeqNP_001001735.1. NM_001001735.1. [Q6P2L6-2]
XP_006509168.1. XM_006509105.1. [Q6P2L6-2]
UniGeneMm.217337.

3D structure databases

ProteinModelPortalQ6P2L6.
SMRQ6P2L6. Positions 262-332, 700-748, 750-834, 919-1288, 1321-1415.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid231497. 1 interaction.
IntActQ6P2L6. 2 interactions.
MINTMINT-4110979.

PTM databases

PhosphoSiteQ6P2L6.

Proteomic databases

PaxDbQ6P2L6.
PRIDEQ6P2L6.

Protocols and materials databases

DNASU234135.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000146919; ENSMUSP00000115470; ENSMUSG00000054823. [Q6P2L6-2]
ENSMUST00000155861; ENSMUSP00000117596; ENSMUSG00000054823. [Q6P2L6-2]
GeneID234135.
KEGGmmu:234135.
UCSCuc009lgk.1. mouse. [Q6P2L6-4]
uc009lgm.1. mouse. [Q6P2L6-2]
uc009lgp.1. mouse. [Q6P2L6-3]

Organism-specific databases

CTD54904.
MGIMGI:2142581. Whsc1l1.

Phylogenomic databases

eggNOGCOG2940.
GeneTreeENSGT00740000114921.
HOGENOMHOG000230893.
HOVERGENHBG079979.
InParanoidQ6P2L6.
KOK11425.
OrthoDBEOG7Z69BG.

Gene expression databases

ArrayExpressQ6P2L6.
BgeeQ6P2L6.
GenevestigatorQ6P2L6.

Family and domain databases

Gene3D3.30.40.10. 3 hits.
InterProIPR006560. AWS.
IPR003616. Post-SET_dom.
IPR000313. PWWP_dom.
IPR001214. SET_dom.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF00855. PWWP. 2 hits.
PF00856. SET. 1 hit.
[Graphical view]
SMARTSM00570. AWS. 1 hit.
SM00249. PHD. 5 hits.
SM00508. PostSET. 1 hit.
SM00293. PWWP. 2 hits.
SM00317. SET. 1 hit.
[Graphical view]
SUPFAMSSF57903. SSF57903. 3 hits.
PROSITEPS51215. AWS. 1 hit.
PS50868. POST_SET. 1 hit.
PS50812. PWWP. 2 hits.
PS50280. SET. 1 hit.
PS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSWHSC1L1. mouse.
NextBio382034.
PROQ6P2L6.
SOURCESearch...

Entry information

Entry nameNSD3_MOUSE
AccessionPrimary (citable) accession number: Q6P2L6
Secondary accession number(s): Q3TDS4 expand/collapse secondary AC list , Q3U0L8, Q3V131, Q8BJT3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 31, 2006
Last modified: July 9, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot