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Q6P2H3 (CEP85_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Centrosomal protein of 85 kDa

Short name=Cep85
Alternative name(s):
Coiled-coil domain-containing protein 21
Gene names
Name:CEP85
Synonyms:CCDC21
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length762 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Cytoplasmcytoskeletonspindle pole. Nucleusnucleolus. Note: Localizes to centrosomes and nucleolus in interphase. Upon entry into mitosis, relocates from nucleolus and accumulates at spindle poles. Ref.8

Sequence similarities

Belongs to the CEP85 family.

Sequence caution

The sequence BAB15203.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAH71541.2 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAI15849.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentCytoplasm
Cytoskeleton
Nucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainCoiled coil
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcentrosome

Inferred from direct assay Ref.8. Source: UniProtKB

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-KW

nucleolus

Inferred from direct assay Ref.8. Source: UniProtKB

spindle pole

Inferred from direct assay Ref.8. Source: UniProtKB

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 18985028. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Q9WMX22EBI-2808308,EBI-6863741From a different organism.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6P2H3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6P2H3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     710-711: SA → S
Isoform 3 (identifier: Q6P2H3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-568: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 762762Centrosomal protein of 85 kDa
PRO_0000233660

Regions

Coiled coil334 – 657324 Potential
Coiled coil723 – 75028 Potential

Amino acid modifications

Modified residue171Phosphoserine Ref.6
Modified residue6231Phosphoserine Ref.7

Natural variations

Alternative sequence1 – 568568Missing in isoform 3.
VSP_026435
Alternative sequence710 – 7112SA → S in isoform 2.
VSP_018147
Natural variant481R → H.
Corresponds to variant rs35831900 [ dbSNP | Ensembl ].
VAR_053938
Natural variant2131S → N.
Corresponds to variant rs3795686 [ dbSNP | Ensembl ].
VAR_053939
Natural variant5421A → T.
Corresponds to variant rs7550997 [ dbSNP | Ensembl ].
VAR_033665
Natural variant6681Q → H.
Corresponds to variant rs36013141 [ dbSNP | Ensembl ].
VAR_053940

Experimental info

Sequence conflict258 – 2603LSK → HSSQ in BAB14794. Ref.1
Sequence conflict5611S → G in BAB15203. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: E6003FD530D8ACA8

FASTA76285,639
        10         20         30         40         50         60 
MAMQEKYPTE GISHVTSPSS DVIQKGSSLG TEWQTPVISE PFRSRFSRCS SVADSGDTAI 

        70         80         90        100        110        120 
GTSCSDIAED FCSSSGSPPF QPIKSHVTIP TAHVMPSTLG TSPAKPNSTP VGPSSSKLPL 

       130        140        150        160        170        180 
SGLAESVGMT RNGDLGAMKH SPGLSRDLMY FSGATGENGI EQSWFPAVGH ERQEEARKFD 

       190        200        210        220        230        240 
IPSMESTLNQ SAMMETLYSD PHHRVRFHNP RTSTSKELYR VLPEAKKAPG SGAVFERNGP 

       250        260        270        280        290        300 
HSNSSGVLPL GLQPAPGLSK PLPSQVWQPS PDTWHPREQS CELSTCRQQL ELIRLQMEQM 

       310        320        330        340        350        360 
QLQNGAICHH PAAFGPSLPI LEPAQWISIL NSNEHLLKEK ELLIDKQRKH ISQLEQKVRE 

       370        380        390        400        410        420 
SELQVHSALL GRPAPFGDVC LLRLQELQRE NTFLRAQFAQ KTEALSREKI DLEKKLSASE 

       430        440        450        460        470        480 
VEVQLIRESL KVALQKHSEE VKKQEERVKG RDKHINNLKK KCQKESEQNR EKQQRIETLE 

       490        500        510        520        530        540 
RYLADLPTLE DHQKQSQQLK DSELKSTELQ EKVTELESLL EETQAICREK EIQLESLRQR 

       550        560        570        580        590        600 
EAEFSSAGHS LQDKQSVEET SGEGPEVEME SWQKRYDSLQ KIVEKQQQKM DQLRSQVQSL 

       610        620        630        640        650        660 
EQEVAQEEGT SQALREEAQR RDSALQQLRT AVKELSVQNQ DLIEKNLTLQ EHLRQAQPGS 

       670        680        690        700        710        720 
PPSPDTAQLA LELHQELASC LQDLQAVCSI VTQRAQGHDP NLSLLLGIHS AQHPETQLDL 

       730        740        750        760 
QKPDVIKRKL EEVQQLRRDI EDLRTTMSDR YAQDMGENCV TQ 

« Hide

Isoform 2 [UniParc].

Checksum: 0B8AC50D8C8EFC4E
Show »

FASTA76185,568
Isoform 3 [UniParc].

Checksum: 7835587B0D08A905
Show »

FASTA19422,242

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 408-762 (ISOFORM 1).
Tissue: Hepatoma and Retinoblastoma.
[2]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Placenta and Testis.
[5]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 328-762 (ISOFORM 2).
Tissue: Testis.
[6]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-623, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods."
Jakobsen L., Vanselow K., Skogs M., Toyoda Y., Lundberg E., Poser I., Falkenby L.G., Bennetzen M., Westendorf J., Nigg E.A., Uhlen M., Hyman A.A., Andersen J.S.
EMBO J. 30:1520-1535(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK024038 mRNA. Translation: BAB14794.1.
AK025653 mRNA. Translation: BAB15203.1. Different initiation.
AL355877, AL451139 Genomic DNA. Translation: CAH71540.2.
AL355877, AL451139 Genomic DNA. Translation: CAH71541.2. Sequence problems.
AL451139, AL355877 Genomic DNA. Translation: CAI15848.2.
AL451139, AL355877 Genomic DNA. Translation: CAI15849.2. Sequence problems.
CH471059 Genomic DNA. Translation: EAX07834.1.
CH471059 Genomic DNA. Translation: EAX07835.1.
BC019902 mRNA. Translation: AAH19902.1.
BC064528 mRNA. Translation: AAH64528.1.
AL133609 mRNA. Translation: CAB63740.1.
CCDSCCDS277.1. [Q6P2H3-1]
PIRT43448.
RefSeqNP_001268446.1. NM_001281517.1.
NP_001268447.1. NM_001281518.1. [Q6P2H3-3]
NP_073615.2. NM_022778.3. [Q6P2H3-1]
XP_005246026.1. XM_005245969.1. [Q6P2H3-2]
XP_006710885.1. XM_006710822.1. [Q6P2H3-1]
XP_006710886.1. XM_006710823.1. [Q6P2H3-1]
UniGeneHs.109051.
Hs.63795.

3D structure databases

ProteinModelPortalQ6P2H3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122301. 6 interactions.
IntActQ6P2H3. 14 interactions.
MINTMINT-7034519.
STRING9606.ENSP00000252992.

PTM databases

PhosphoSiteQ6P2H3.

Polymorphism databases

DMDM74737212.

Proteomic databases

MaxQBQ6P2H3.
PaxDbQ6P2H3.
PRIDEQ6P2H3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000252992; ENSP00000252992; ENSG00000130695. [Q6P2H3-1]
ENST00000476272; ENSP00000432354; ENSG00000130695. [Q6P2H3-2]
GeneID64793.
KEGGhsa:64793.
UCSCuc001blr.3. human. [Q6P2H3-2]
uc001bls.1. human. [Q6P2H3-1]
uc001blt.1. human. [Q6P2H3-3]

Organism-specific databases

CTD64793.
GeneCardsGC01P026561.
HGNCHGNC:25309. CEP85.
HPAHPA028252.
neXtProtNX_Q6P2H3.
PharmGKBPA142672180.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG150793.
HOVERGENHBG058148.
InParanoidQ6P2H3.
KOK16766.
OMAEQSWFPA.
OrthoDBEOG75TMBD.
PhylomeDBQ6P2H3.
TreeFamTF331041.

Gene expression databases

ArrayExpressQ6P2H3.
BgeeQ6P2H3.
CleanExHS_CCDC21.
GenevestigatorQ6P2H3.

Family and domain databases

ProtoNetSearch...

Other

GenomeRNAi64793.
NextBio66858.
PROQ6P2H3.

Entry information

Entry nameCEP85_HUMAN
AccessionPrimary (citable) accession number: Q6P2H3
Secondary accession number(s): D3DPK4 expand/collapse secondary AC list , Q5VY68, Q5VY70, Q9H6Q1, Q9H828, Q9UF52
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM