Q6P2H3 (CEP85_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Centrosomal protein of 85 kDa

Alternative name(s):
Coiled-coil domain-containing protein 21

Gene names

Name:	CEP85
Synonyms:	CCDC21

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	762 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cytoplasm › cytoskeleton › centrosome. Cytoplasm › cytoskeleton › spindle pole. Nucleus › nucleolus. Note: Localizes to centrosomes and nucleolus in interphase. Upon entry into mitosis, relocates from nucleolus and accumulates at spindle poles. Ref.8
Sequence similarities	Belongs to the CEP85 family.
Sequence caution	The sequence BAB15203.1 differs from that shown. Reason: Erroneous initiation. The sequence CAH71541.2 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI15849.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	centrosome Inferred from direct assay Ref.8. Source: UniProtKB cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW nucleolus Inferred from direct assay Ref.8. Source: UniProtKB spindle pole Inferred from direct assay Ref.8. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6P2H3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6P2H3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     710-711: SA → S

Isoform 3 (identifier: Q6P2H3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-568: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 762	762	Centrosomal protein of 85 kDa		PRO_0000233660
Regions
	Coiled coil	334 – 657	324	Potential
	Coiled coil	723 – 750	28	Potential
Amino acid modifications
	Modified residue	17	1	Phosphoserine Ref.6
	Modified residue	623	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	1 – 568	568	Missing in isoform 3.		VSP_026435
	Alternative sequence	710 – 711	2	SA → S in isoform 2.		VSP_018147
	Natural variant	48	1	R → H. Corresponds to variant rs35831900 [ dbSNP | Ensembl ].		VAR_053938
	Natural variant	213	1	S → N. Corresponds to variant rs3795686 [ dbSNP | Ensembl ].		VAR_053939
	Natural variant	542	1	A → T. Corresponds to variant rs7550997 [ dbSNP | Ensembl ].		VAR_033665
	Natural variant	668	1	Q → H. Corresponds to variant rs36013141 [ dbSNP | Ensembl ].		VAR_053940
Experimental info
	Sequence conflict	258 – 260	3	LSK → HSSQ in BAB14794. Ref.1
	Sequence conflict	561	1	S → G in BAB15203. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: E6003FD530D8ACA8	FASTA	762	85,639
	10 20 30 40 50 60 MAMQEKYPTE GISHVTSPSS DVIQKGSSLG TEWQTPVISE PFRSRFSRCS SVADSGDTAI 70 80 90 100 110 120 GTSCSDIAED FCSSSGSPPF QPIKSHVTIP TAHVMPSTLG TSPAKPNSTP VGPSSSKLPL 130 140 150 160 170 180 SGLAESVGMT RNGDLGAMKH SPGLSRDLMY FSGATGENGI EQSWFPAVGH ERQEEARKFD 190 200 210 220 230 240 IPSMESTLNQ SAMMETLYSD PHHRVRFHNP RTSTSKELYR VLPEAKKAPG SGAVFERNGP 250 260 270 280 290 300 HSNSSGVLPL GLQPAPGLSK PLPSQVWQPS PDTWHPREQS CELSTCRQQL ELIRLQMEQM 310 320 330 340 350 360 QLQNGAICHH PAAFGPSLPI LEPAQWISIL NSNEHLLKEK ELLIDKQRKH ISQLEQKVRE 370 380 390 400 410 420 SELQVHSALL GRPAPFGDVC LLRLQELQRE NTFLRAQFAQ KTEALSREKI DLEKKLSASE 430 440 450 460 470 480 VEVQLIRESL KVALQKHSEE VKKQEERVKG RDKHINNLKK KCQKESEQNR EKQQRIETLE 490 500 510 520 530 540 RYLADLPTLE DHQKQSQQLK DSELKSTELQ EKVTELESLL EETQAICREK EIQLESLRQR 550 560 570 580 590 600 EAEFSSAGHS LQDKQSVEET SGEGPEVEME SWQKRYDSLQ KIVEKQQQKM DQLRSQVQSL 610 620 630 640 650 660 EQEVAQEEGT SQALREEAQR RDSALQQLRT AVKELSVQNQ DLIEKNLTLQ EHLRQAQPGS 670 680 690 700 710 720 PPSPDTAQLA LELHQELASC LQDLQAVCSI VTQRAQGHDP NLSLLLGIHS AQHPETQLDL 730 740 750 760 QKPDVIKRKL EEVQQLRRDI EDLRTTMSDR YAQDMGENCV TQ
	Isoform 2 [UniParc]. Checksum: 0B8AC50D8C8EFC4E	FASTA	761	85,568
	10 20 30 40 50 60 MAMQEKYPTE GISHVTSPSS DVIQKGSSLG TEWQTPVISE PFRSRFSRCS SVADSGDTAI 70 80 90 100 110 120 GTSCSDIAED FCSSSGSPPF QPIKSHVTIP TAHVMPSTLG TSPAKPNSTP VGPSSSKLPL 130 140 150 160 170 180 SGLAESVGMT RNGDLGAMKH SPGLSRDLMY FSGATGENGI EQSWFPAVGH ERQEEARKFD 190 200 210 220 230 240 IPSMESTLNQ SAMMETLYSD PHHRVRFHNP RTSTSKELYR VLPEAKKAPG SGAVFERNGP 250 260 270 280 290 300 HSNSSGVLPL GLQPAPGLSK PLPSQVWQPS PDTWHPREQS CELSTCRQQL ELIRLQMEQM 310 320 330 340 350 360 QLQNGAICHH PAAFGPSLPI LEPAQWISIL NSNEHLLKEK ELLIDKQRKH ISQLEQKVRE 370 380 390 400 410 420 SELQVHSALL GRPAPFGDVC LLRLQELQRE NTFLRAQFAQ KTEALSREKI DLEKKLSASE 430 440 450 460 470 480 VEVQLIRESL KVALQKHSEE VKKQEERVKG RDKHINNLKK KCQKESEQNR EKQQRIETLE 490 500 510 520 530 540 RYLADLPTLE DHQKQSQQLK DSELKSTELQ EKVTELESLL EETQAICREK EIQLESLRQR 550 560 570 580 590 600 EAEFSSAGHS LQDKQSVEET SGEGPEVEME SWQKRYDSLQ KIVEKQQQKM DQLRSQVQSL 610 620 630 640 650 660 EQEVAQEEGT SQALREEAQR RDSALQQLRT AVKELSVQNQ DLIEKNLTLQ EHLRQAQPGS 670 680 690 700 710 720 PPSPDTAQLA LELHQELASC LQDLQAVCSI VTQRAQGHDP NLSLLLGIHS QHPETQLDLQ 730 740 750 760 KPDVIKRKLE EVQQLRRDIE DLRTTMSDRY AQDMGENCVT Q
	Isoform 3 [UniParc]. Checksum: 7835587B0D08A905	FASTA	194	22,242
	10 20 30 40 50 60 MESWQKRYDS LQKIVEKQQQ KMDQLRSQVQ SLEQEVAQEE GTSQALREEA QRRDSALQQL 70 80 90 100 110 120 RTAVKELSVQ NQDLIEKNLT LQEHLRQAQP GSPPSPDTAQ LALELHQELA SCLQDLQAVC 130 140 150 160 170 180 SIVTQRAQGH DPNLSLLLGI HSAQHPETQL DLQKPDVIKR KLEEVQQLRR DIEDLRTTMS 190 DRYAQDMGEN CVTQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 408-762 (ISOFORM 1). Tissue: Hepatoma and Retinoblastoma.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Placenta and Testis.
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 328-762 (ISOFORM 2). Tissue: Testis.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-623, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods." Jakobsen L., Vanselow K., Skogs M., Toyoda Y., Lundberg E., Poser I., Falkenby L.G., Bennetzen M., Westendorf J., Nigg E.A., Uhlen M., Hyman A.A., Andersen J.S. EMBO J. 30:1520-1535(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK024038 mRNA. Translation: BAB14794.1. AK025653 mRNA. Translation: BAB15203.1. Different initiation. AL355877, AL451139 Genomic DNA. Translation: CAH71540.2. AL355877, AL451139 Genomic DNA. Translation: CAH71541.2. Sequence problems. AL451139, AL355877 Genomic DNA. Translation: CAI15848.2. AL451139, AL355877 Genomic DNA. Translation: CAI15849.2. Sequence problems. CH471059 Genomic DNA. Translation: EAX07834.1. CH471059 Genomic DNA. Translation: EAX07835.1. BC019902 mRNA. Translation: AAH19902.1. BC064528 mRNA. Translation: AAH64528.1. AL133609 mRNA. Translation: CAB63740.1.
IPI	IPI00009702. IPI00550977. IPI00847495.
PIR	T43448.
RefSeq	NP_073615.2. NM_022778.2.
UniGene	Hs.63795.
3D structure databases
ProteinModelPortal	Q6P2H3.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6P2H3. 11 interactions.
MINT	MINT-7034519.
STRING	9606.ENSP00000252992.
PTM databases
PhosphoSite	Q6P2H3.
Polymorphism databases
DMDM	74737212.
Proteomic databases
PaxDb	Q6P2H3.
PRIDE	Q6P2H3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000252992; ENSP00000252992; ENSG00000130695. ENST00000476272; ENSP00000432354; ENSG00000130695.
GeneID	64793.
KEGG	hsa:64793.
UCSC	uc001blr.3. human. uc001bls.1. human. uc001blt.1. human.
Organism-specific databases
CTD	64793.
GeneCards	GC01P026561.
HGNC	HGNC:25309. CEP85.
HPA	HPA028252.
neXtProt	NX_Q6P2H3.
PharmGKB	PA142672180.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG150793.
HOVERGEN	HBG058148.
InParanoid	Q6P2H3.
KO	K16766.
OMA	KGRDKHI.
OrthoDB	EOG4PNXGB.
PhylomeDB	Q6P2H3.
Gene expression databases
ArrayExpress	Q6P2H3.
Bgee	Q6P2H3.
CleanEx	HS_CCDC21.
Genevestigator	Q6P2H3.
GermOnline	ENSG00000130695. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other
GenomeRNAi	64793.
NextBio	66858.

Entry information

Entry name

CEP85_HUMAN

Accession

Primary (citable) accession number: Q6P2H3
Secondary accession number(s): D3DPK4 , Q5VY68, Q5VY70, Q9H6Q1, Q9H828, Q9UF52

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.