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Protein

Mediator of RNA polymerase II transcription subunit 27

Gene

MED27

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1989781. PPARA activates gene expression.
R-HSA-212436. Generic Transcription Pathway.
R-HSA-381340. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 27
Alternative name(s):
Cofactor required for Sp1 transcriptional activation subunit 8
Short name:
CRSP complex subunit 8
Mediator complex subunit 27
P37 TRAP/SMCC/PC2 subunit
Transcriptional coactivator CRSP34
Gene namesi
Name:MED27
Synonyms:CRSP34Imported, CRSP8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:2377. MED27.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • mediator complex Source: Ensembl
  • nucleolus Source: HPA
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
  • transcription factor complex Source: MGI
  • ubiquitin ligase complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000160563.
PharmGKBiPA162395634.

Polymorphism and mutation databases

BioMutaiMED27.
DMDMi74737195.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000793611 – 311Mediator of RNA polymerase II transcription subunit 27Add BLAST311

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei132PhosphoserineCombined sources1
Modified residuei134N6-methyllysineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ6P2C8.
MaxQBiQ6P2C8.
PaxDbiQ6P2C8.
PeptideAtlasiQ6P2C8.
PRIDEiQ6P2C8.

PTM databases

iPTMnetiQ6P2C8.
PhosphoSitePlusiQ6P2C8.

Expressioni

Gene expression databases

BgeeiENSG00000160563.
CleanExiHS_MED27.
ExpressionAtlasiQ6P2C8. baseline and differential.
GenevisibleiQ6P2C8. HS.

Organism-specific databases

HPAiHPA007002.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MED17Q9NVC62EBI-394603,EBI-394562
Med20Q9R0X02EBI-394603,EBI-398698From a different organism.
MED22Q155283EBI-394603,EBI-394687
Med30Q9CQI92EBI-394603,EBI-309220From a different organism.
MED6O755862EBI-394603,EBI-394624
Med8Q9D7W52EBI-394603,EBI-7990252From a different organism.

Protein-protein interaction databases

BioGridi114832. 43 interactors.
DIPiDIP-31465N.
IntActiQ6P2C8. 25 interactors.
STRINGi9606.ENSP00000292035.

Structurei

3D structure databases

ProteinModelPortaliQ6P2C8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 27 family.Curated

Phylogenomic databases

eggNOGiENOG410IFPB. Eukaryota.
ENOG410ZHQY. LUCA.
GeneTreeiENSGT00390000012207.
HOVERGENiHBG104472.
InParanoidiQ6P2C8.
KOiK15170.
OMAiCSQKRQR.
OrthoDBiEOG091G0BWJ.
PhylomeDBiQ6P2C8.
TreeFamiTF323728.

Family and domain databases

InterProiIPR021627. Mediator_Med27.
[Graphical view]
PfamiPF11571. Med27. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 PublicationCurated2 Publications (identifier: Q6P2C8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADVINVSVN LEAFSQAISA IQALRSSVSR VFDCLKDGMR NKETLEGREK
60 70 80 90 100
AFIAHFQDNL HSVNRDLNEL ERLSNLVGKP SENHPLHNSG LLSLDPVQDK
110 120 130 140 150
TPLYSQLLQA YKWSNKLQYH AGLASGLLNQ QSLKRSANQM GVSAKRRPKA
160 170 180 190 200
QPTTLVLPPQ YVDDVISRID RMFPEMSIHL SRPNGTSAML LVTLGKVLKV
210 220 230 240 250
IVVMRSLFID RTIVKGYNEN VYTEDGKLDI WSKSNYQVFQ KVTDHATTAL
260 270 280 290 300
LHYQLPQMPD VVVRSFMTWL RSYIKLFQAP CQRCGKFLQD GLPPTWRDFR
310
TLEAFHDTCR Q
Length:311
Mass (Da):35,432
Last modified:July 5, 2004 - v1
Checksum:i996D675993E393CB
GO
Isoform 21 Publication (identifier: Q6P2C8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     192-227: Missing.

Show »
Length:275
Mass (Da):31,351
Checksum:i375CDE48668D429F
GO
Isoform 3 (identifier: Q6P2C8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     117-130: LQYHAGLASGLLNQ → KEQLSIPRIFHWKV
     131-311: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:130
Mass (Da):14,826
Checksum:iA6882C84B5148C53
GO

Sequence cautioni

The sequence AAH02878 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAH02878 differs from that shown. Reason: Erroneous termination at position 109. Translated as Gln.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti64N → D in AAZ13763 (Ref. 6) Curated1
Sequence conflicti79K → E in AAZ13763 (Ref. 6) Curated1
Sequence conflicti94L → S in AAD12721 (PubMed:9989412).Curated1
Sequence conflicti207L → C in AAD12721 (PubMed:9989412).Curated1
Sequence conflicti235N → D in AAZ13763 (Ref. 6) Curated1
Sequence conflicti279A → G in AAD12721 (PubMed:9989412).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_055411117 – 130LQYHA…GLLNQ → KEQLSIPRIFHWKV in isoform 3. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_055412131 – 311Missing in isoform 3. 1 PublicationAdd BLAST181
Alternative sequenceiVSP_051869192 – 227Missing in isoform 2. 3 PublicationsAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BQ189678 Genomic DNA. No translation available.
AL603649 Genomic DNA. No translation available.
AL513102 Genomic DNA. No translation available.
AL160271 Genomic DNA. No translation available.
AL713892 Genomic DNA. No translation available.
BC002878 mRNA. Translation: AAH02878.1. Sequence problems.
BC064608 mRNA. Translation: AAH64608.1.
AF104252 mRNA. Translation: AAD12721.1.
AF230382 mRNA. Translation: AAF37290.1.
DQ099387 mRNA. Translation: AAZ13763.1.
CCDSiCCDS59153.1. [Q6P2C8-2]
CCDS6945.1. [Q6P2C8-1]
CCDS69689.1. [Q6P2C8-4]
RefSeqiNP_001240810.1. NM_001253881.1. [Q6P2C8-2]
NP_001240811.1. NM_001253882.1. [Q6P2C8-4]
NP_004260.2. NM_004269.3. [Q6P2C8-1]
UniGeneiHs.374262.

Genome annotation databases

EnsembliENST00000292035; ENSP00000292035; ENSG00000160563. [Q6P2C8-1]
ENST00000357028; ENSP00000349530; ENSG00000160563. [Q6P2C8-2]
ENST00000474263; ENSP00000477136; ENSG00000160563. [Q6P2C8-4]
GeneIDi9442.
KEGGihsa:9442.
UCSCiuc004cbe.3. human. [Q6P2C8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BQ189678 Genomic DNA. No translation available.
AL603649 Genomic DNA. No translation available.
AL513102 Genomic DNA. No translation available.
AL160271 Genomic DNA. No translation available.
AL713892 Genomic DNA. No translation available.
BC002878 mRNA. Translation: AAH02878.1. Sequence problems.
BC064608 mRNA. Translation: AAH64608.1.
AF104252 mRNA. Translation: AAD12721.1.
AF230382 mRNA. Translation: AAF37290.1.
DQ099387 mRNA. Translation: AAZ13763.1.
CCDSiCCDS59153.1. [Q6P2C8-2]
CCDS6945.1. [Q6P2C8-1]
CCDS69689.1. [Q6P2C8-4]
RefSeqiNP_001240810.1. NM_001253881.1. [Q6P2C8-2]
NP_001240811.1. NM_001253882.1. [Q6P2C8-4]
NP_004260.2. NM_004269.3. [Q6P2C8-1]
UniGeneiHs.374262.

3D structure databases

ProteinModelPortaliQ6P2C8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114832. 43 interactors.
DIPiDIP-31465N.
IntActiQ6P2C8. 25 interactors.
STRINGi9606.ENSP00000292035.

PTM databases

iPTMnetiQ6P2C8.
PhosphoSitePlusiQ6P2C8.

Polymorphism and mutation databases

BioMutaiMED27.
DMDMi74737195.

Proteomic databases

EPDiQ6P2C8.
MaxQBiQ6P2C8.
PaxDbiQ6P2C8.
PeptideAtlasiQ6P2C8.
PRIDEiQ6P2C8.

Protocols and materials databases

DNASUi9442.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000292035; ENSP00000292035; ENSG00000160563. [Q6P2C8-1]
ENST00000357028; ENSP00000349530; ENSG00000160563. [Q6P2C8-2]
ENST00000474263; ENSP00000477136; ENSG00000160563. [Q6P2C8-4]
GeneIDi9442.
KEGGihsa:9442.
UCSCiuc004cbe.3. human. [Q6P2C8-1]

Organism-specific databases

CTDi9442.
GeneCardsiMED27.
HGNCiHGNC:2377. MED27.
HPAiHPA007002.
MIMi605044. gene.
neXtProtiNX_Q6P2C8.
OpenTargetsiENSG00000160563.
PharmGKBiPA162395634.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFPB. Eukaryota.
ENOG410ZHQY. LUCA.
GeneTreeiENSGT00390000012207.
HOVERGENiHBG104472.
InParanoidiQ6P2C8.
KOiK15170.
OMAiCSQKRQR.
OrthoDBiEOG091G0BWJ.
PhylomeDBiQ6P2C8.
TreeFamiTF323728.

Enzyme and pathway databases

ReactomeiR-HSA-1989781. PPARA activates gene expression.
R-HSA-212436. Generic Transcription Pathway.
R-HSA-381340. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

ChiTaRSiMED27. human.
GeneWikiiMED27.
GenomeRNAii9442.
PROiQ6P2C8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000160563.
CleanExiHS_MED27.
ExpressionAtlasiQ6P2C8. baseline and differential.
GenevisibleiQ6P2C8. HS.

Family and domain databases

InterProiIPR021627. Mediator_Med27.
[Graphical view]
PfamiPF11571. Med27. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMED27_HUMAN
AccessioniPrimary (citable) accession number: Q6P2C8
Secondary accession number(s): O95401
, Q4F964, Q5VTA4, Q5VTA5, Q9BU57, Q9NYR4, V9GYV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.