Q6P286 (DHTK1_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial
EC=1.2.4.2

Alternative name(s):
Dehydrogenase E1 and transketolase domain-containing protein 1

Gene names

Name:

dhtkd1

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	927 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity.
Catalytic activity	2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2.
Cofactor	Thiamine pyrophosphate By similarity.
Subcellular location	Mitochondrion Potential.
Sequence similarities	Belongs to the alpha-ketoglutarate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Thiamine pyrophosphate
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Cellular_component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	oxoglutarate dehydrogenase (succinyl-transferring) activity Inferred from electronic annotation. Source: EC thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 927		Probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial		PRO_0000307941

Sequences

	Sequence		Length	Mass (Da)
	Q6P286 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 43AEFD67A73A0EF3	FASTA	927	104,104
	10 20 30 40 50 60 MNVFAHRCSS CRRAVVLWRP GLHRLYRTER GVYGYRPKIS AGSQLQESST RGKQAASPTV 70 80 90 100 110 120 DHGLARLVTA YREHGHKAAK INPLFTGQAV MDMVPEIQEI MEILHGPFST TGLLNMGKSE 130 140 150 160 170 180 ATIEEVLAYL DHTYCGQISI ETSQLQNYKE REWFSRRFEE LKQESFSTEE RKHLARLMLE 190 200 210 220 230 240 CQEFDHFLAT KFSTVKRYGG EGAESMMGFF HEMLKMCSFG GVTDVIIGMP HRGRLNLLTG 250 260 270 280 290 300 LLQFPPELMF RKMRGLSEFP ENSPSIGDVL SHLTSSVDLD FGSHRPLHVT MLPNPSHLEA 310 320 330 340 350 360 INPVAVGKTR ARQQSLSDGD YSTESSAQPG DKVVCLQVHG DASISGQGIV TETFTLSNLP 370 380 390 400 410 420 HYRIGGSIHL IVNNQLGYTT PAERGRSSLY SSDVGKIVGC AVIHVNGDDP EEVLRATRLA 430 440 450 460 470 480 VEYQRCFRKD VIIDLLCYRQ WGHNELDEPF FTNPSMYKII RSRKSIPDVY SERLIAEGLM 490 500 510 520 530 540 TEEEATEIRT TYYSKFNDHL SNMTLYSPPS TNLQAHWREM IEPSARTTTW DTGLPADLLK 550 560 570 580 590 600 FIGAKSVEVP EEFKMHSHLL KMHAQSRVQK LQEATKLDWA TAEALAFGSL LCQGFNIRIS 610 620 630 640 650 660 GQDVGRGTFS QRHAMLVCQE TNDTYIPLNH MTPDQKGFLE VSNSALSEEA VLGFEYGMSI 670 680 690 700 710 720 ESPKLLPIWE AQFGDFFNGA QIIFDTFISG GEAKWLLQSG IVILLPHGYD GAGPEHSSCR 730 740 750 760 770 780 IERFLQMCDS TEEGVDGDTV NMFVVHPTTP AQYFHLLRRQ MVRSFRKPLI VASPKMLLRY 790 800 810 820 830 840 PAAVSSLEDI APGKTFRSVI GDSSADPKSV SKVILCSGKH YYALHKQREA LGEQGRSSAI 850 860 870 880 890 900 IRVEELCPFP LEALQQEIHR YPKAKDFIWS QEEPQNMGAW TFVAPRFEKQ LACKLRLVSR 910 920 PALPAPAVGI GTLHQQQQEE ITVKTLS

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC064683 mRNA. Translation: AAH64683.1.
RefSeq	NP_001084395.1. NM_001090926.1.
UniGene	Xl.4843.
3D structure databases
ProteinModelPortal	Q6P286.
ModBase	Search...
Proteomic databases
PRIDE	Q6P286.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	403360.
KEGG	xla:403360.
Organism-specific databases
CTD	55526.
Xenbase	XB-GENE-980614. dhtkd1.
Phylogenomic databases
HOVERGEN	HBG001892.
KO	K15791.
Family and domain databases
InterPro	IPR011603. 2oxoglutarate_DH_E1. IPR001017. DH_E1. IPR005475. Transketolase-like_Pyr-bd. [Graphical view]
PANTHER	PTHR23152. PTHR23152. 1 hit.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. [Graphical view]
PIRSF	PIRSF000157. Oxoglu_dh_E1. 1 hit.
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
TIGRFAMs	TIGR00239. 2oxo_dh_E1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DHTK1_XENLA

Accession

Primary (citable) accession number: Q6P286

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	July 5, 2004
Last modified:	October 3, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program